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Marine Biotechnology

Mariko Iijima, Ko Yasumoto, Jun Yasumoto, Mina Yasumoto-Hirose, Nami Kuniya, Ryota Takeuchi, Masashi Nozaki, Nobuyoshi Nanba, Takashi Nakamura, Mitsuru Jimbo, Shugo Watabe
Coral reef degradation due to various local stresses, such as nutrient enrichment and terrestrial run-off into coastal waters, is an increasing global concern. Inorganic phosphates have been considered to possibly inhibit skeleton formation in corals. Despite many studies available on the effects of nutrients on corals, a clear consensus on how nutrients exert deteriorative effects on corals has not been established satisfactorily. In this study, we examined the effects of phosphates and nitrates on in vitro aragonite CaCO3 formation by using biogenic polyamines and in vivo aragonite formation in the skeleton of juvenile Acropora digitifera corals...
February 12, 2019: Marine Biotechnology
Dan Li Jiang, Xiao Hui Gu, Bi Jun Li, Zong Xian Zhu, Hui Qin, Zi Ning Meng, Hao Ran Lin, Jun Hong Xia
Understanding the genetic mechanism of osmoregulation is important for the improvement of salt tolerance in tilapia. In our previous study, we have identified a major quantitative trait locus (QTL) region located at 23.0 Mb of chrLG18 in a Nile tilapia line by QTL-seq. However, the conservation of these QTLs in other tilapia populations or species is not clear. In this study, we successfully investigated the QTLs associated with salt tolerance in a mass cross population from the GIFT line of Nile tilapia (Oreochromis niloticus) using a ddRAD-seq-based genome-wide association study (GWAS) and in a full-sib family from the Malaysia red tilapia strain (Oreochromis spp) using QTL-seq...
February 8, 2019: Marine Biotechnology
Immacolata Serra, Claudia Capusoni, Francesco Molinari, Loana Musso, Luisa Pellegrino, Concetta Compagno
A screening among marine yeasts was carried out for nitrile hydrolyzing activity. Meyerozyma guilliermondii LM2 (UBOCC-A-214008) was able to efficiently grow on benzonitrile and cyclohexanecarbonitrile (CECN) as sole nitrogen sources. A two-step one-pot method for obtaining cells of M. guilliermondii LM2 (UBOCC-A-214008) endowed with high nitrilase activity was established; the resulting whole cells converted different nitriles with high molar conversions and showed interesting enantioselectivity toward racemic substrates...
January 25, 2019: Marine Biotechnology
Yangping Li, Lingling Zhang, Yajuan Li, Wanru Li, Zhenyi Guo, Ruojiao Li, Xiaoli Hu, Zhenmin Bao, Shi Wang
DNA methylation reprograms during gametogenesis and embryo development, which is essential for germ cell specification and genomic imprinting in mammals. Corresponding process remains poorly investigated in molluscs. Here, we examined global DNA methylation level in the gonads of scallop Patinopecten yessoensis during gametogenesis and in embryos/larvae at different stages. DNA methylation level fluctuates during gametogenesis and early development, peaking at proliferative stage of ovary, growing stage of testis, and in blastulae...
January 24, 2019: Marine Biotechnology
Ding Ye, Lin Zhu, Qifeng Zhang, Feng Xiong, Houpeng Wang, Xiaosi Wang, Mudan He, Zuoyan Zhu, Yonghua Sun
Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe the generation and characterization of a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)ihb327Tg , which specifically labels the whole lifetime of germ cells, i.e., from primordial germ cells (PGCs) at shield stage to the oogonia and early stage of oocytes in the ovary and to the early stage of spermatogonia, spermatocyte, and spermatid in the testis. By using this transgenic line, we carefully observed the numbers of PGCs from early embryonic stage to juvenile stage and the differentiation process of ovary and testis...
January 22, 2019: Marine Biotechnology
Can Jin, Jing-Ying Zhao, Xiao-Jun Liu, Jia-Le Li
Matrix proteins regulate crystal nucleation, morphology, and polymorphism during pearl biomineralization and have significant correlations with pearl quality traits in nucleated pearls. However, there is little information about the connection between pearl quality traits and matrix proteins in non-nucleated pearls. In this study, we analyzed CaCO3 deposition during the first month of non-nucleated pearl formation and examined the expression patterns of ten shell matrix protein genes (Hcperlucin, hic31, silkmapin, hic22, hic74, hic52, HcTyr, HcCA3, hic24 and Hc-upsalin) in the pearl sac of Hyriopsis cumingii...
January 18, 2019: Marine Biotechnology
Qinbo Qin, Liu Cao, Yude Wang, Li Ren, Qiwen Liu, Yuwei Zhou, Chongqing Wang, Huan Qin, Chun Zhao, Shaojun Liu
The article "Rapid Genomic and Genetic Changes in the First Generation of Autotetraploid Lineages Derived from Distant Hybridization of Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂)" was originally published electronically on the publisher's internet portal (currently SpringerLink) on 13 November 2018 with open access.
January 18, 2019: Marine Biotechnology
Ke-Yi Ma, Shu-Hui Yu, Yu-Xin Du, Shi-Qing Feng, Liang-Jie Qiu, Dai-Yi Ke, Mei-Zhong Luo, Gao-Feng Qiu
Knowledge on sex determination has proven valuable for commercial production of the prawn Macrobrachium rosenbergii due to sex dimorphism of the male and female individuals. Previous studies indicated that prawn sex is determined by a ZW-ZZ chromosomal system, but no genomic information is available for the sex chromosome. Herein, we constructed a genomic bacterial artificial chromosome (BAC) library and identified the ZW-derived BAC clones for initial analysis of the sex chromosomal DNA sequence. The arrayed BAC library contains 200,448 clones with average insert size of 115...
January 10, 2019: Marine Biotechnology
Wanwan Zhang, Peng Jia, Wei Liu, Kuntong Jia, Meisheng Yi
Nervous necrosis virus (NNV), one of the most prevalent fish pathogens, has caused significant losses in both yield and economy to the aquaculture. Host factors involved in NNV infection remain to be identified due to the lack of ideal model for the study of NNV and host interaction. Haploid stem cells have proven to be ideal materials in genetic screens. Here, we generated a cell line HX1G1 (simply named G1) with the activity against red-spotted grouper nervous necrosis virus (RGNNV) by N-ethyl-N-nitrosourea (ENU)-mediated whole genome random mutagenesis from the haploid embryonic stem cell HX1a, a cell clone from haploid cell line HX1 that we previously derived from the medaka fish...
January 8, 2019: Marine Biotechnology
Xiangcheng Yuan, Hui Huang, Weihua Zhou, Yajuan Guo, Tao Yuan, Sheng Liu
Recent studies have indicated that various corals might have different degrees of resistance to elevated CO2 levels. However, the underlying molecular mechanism accounting for these differences is still poorly understood. In this study, RNA-seq data were analyzed to identify differentially expressed genes in two coral species (Acropora austera and Acropora cerealis) in response to high CO2 levels. The calcification rates were higher in high CO2 treatment than the control in A. austera, but was not significantly different in A...
January 5, 2019: Marine Biotechnology
Qi Yin, Jinyou Liang, Weipeng Zhang, Lv Zhang, Zhang-Li Hu, Yu Zhang, Ying Xu
Bacterial biofilm can cause nosocomial recurrent infections and implanted device secondary infections in patients and strongly promotes development of pathogenic drug resistance in clinical treatments. Butenolide is an effective anti-macrofouling compound derived from a marine Streptomyces sp., but its antibiofilm efficacy remains largely unexplored. In the present study, the antibiofilm activities of butenolide were examined using biofilms formed by both Gram-positive and Gram-negative pathogenic model species...
January 5, 2019: Marine Biotechnology
Xiaofan Zhang, Shaochen Pang, Chengjie Liu, Houpeng Wang, Ding Ye, Zuoyan Zhu, Yonghua Sun
Omega-3 polyunsaturated fatty acids (n-3 PUFAs), such as eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), are essential for neural development and human health. The n-3 PUFAs are mainly obtained from marine fish by dietary intake. Freshwater fish species usually contain low level of n-3 PUFAs due to the lack of n-3 PUFAs in their food chain. In this study, we report on the substantial production of EPA and DHA in a globally important freshwater fish species, common carp (Cyprinus carpio)...
December 26, 2018: Marine Biotechnology
M Dhaneesha, O Hasin, K C Sivakumar, R Ravinesh, C Benjamin Naman, S Carmeli, T P Sajeevan
A sponge-associated actinomycete (strain MCCB267) was isolated from a marine sponge Mycale sp. collected in the Indian Ocean off the Southeast coast of India. Phylogenetic studies of this strain using 16S rRNA gene sequencing showed high sequence similarity to Streptomyces zhaozhouensis. However, strain MCCB267 showed distinct physiological and biochemical characteristic features and was thus designated as S. zhaozhouensis subsp. mycale. subsp. nov. A cytotoxicity-guided fractionation of the crude ethyl acetate extract of strain MCCB267 culture medium yielded four pure compounds belonging to the polycyclic tetramate macrolactam (PTM) family of natural products: ikarugamycin (IK) (1), clifednamide A (CF) (2), 30-oxo-28-N-methylikarugamycin (OI) (3), and 28-N-methylikarugamycin (MI) (4)...
December 13, 2018: Marine Biotechnology
Xuemei Chen, Yueru Chen, Xiaotong Shen, Jianwei Zuo, Huarong Guo
This study first reported the improvement and application of lentivirus-mediated gene transfer and expression system in shrimp cells. After modified by the inclusion of two envelope proteins (VP19 and VP28) of shrimp white spot syndrome virus (WSSV) into the envelope of the packaged lentivirus, and insertion of a truncated promoter of immediate-early gene 1 (Pie1-504 ) of shrimp WSSV virus into the lentiviral reporter plasmid, the second-generation lentiviral expression system (pLVX-PEF1α -IRES-mCherry, psPAX2, and PMD2...
December 13, 2018: Marine Biotechnology
Vijaya Raghavan Rangamaran, Venkat Kumar Shanmugam
Biocalcification or microbially induced carbonate precipitation (MICP) is gaining attention from the research fraternity, primarily ascribed to their eco-friendly applications. Bacterial strains have been isolated from various sources and their ability to precipitate carbonate has been studied extensively. In spite of the fact that the deep-sea environment is a potential source for bioprospecting, meager reports exist on the isolation of biocalcifying bacterial strains from deep-sea. In this study, a deep-sea sediment sample obtained from off-Barren Island coast in the Andaman Sea was investigated for biocalcifying strains...
December 10, 2018: Marine Biotechnology
Yufeng Si, Haishen Wen, Shaojun Du
Myoblast fusion is a vital step for skeletal muscle development, growth, and regeneration. Loss of Jamb, Jamc, or Myomaker (Mymk) function impaired myoblast fusion in zebrafish embryos. In addition, mymk mutation hampered fish muscle growth. However, the effect of Jamb and Jamc deficiency on fish muscle growth is not clear. Moreover, whether jamb;jamc and jamb;mymk double mutations have stronger effects on myoblast fusion and muscle growth remains to be investigated. Here, we characterized the muscle development and growth in jamb, jamc, and mymk single and double mutants in zebrafish...
November 22, 2018: Marine Biotechnology
Carolina Shene, Marcelo Garcés, Daniela Vergara, Jhonatan Peña, Stéphane Claverol, Mónica Rubilar, Allison Leyton
Total lipids and docosahexaenoic acid (DHA) production by a Chilean isolated thraustochytrid were evaluated under different growth conditions in shake flasks. The analyzed strain was identified as Thraustochytrium striatum according to an 18S rRNA gene sequence analysis. The strain (T. striatum AL16) showed negligible growth in media prepared with artificial seawater at concentrations lower than 50% v/v and pH lower than 5. Maltose and starch were better carbon sources for growth than glucose. DHA content of the biomass grown with maltose (60 g L-1 ) was doubled by increasing the agitation rate from 150 to 250 rpm...
November 19, 2018: Marine Biotechnology
Thu Aung, Hong Jiang, Cheng-Cheng Chen, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi, Zhe Chi
Aureobasidium melanogenum strain 11-1 with a high laccase activity was isolated from a mangrove ecosystem. Under the optimal conditions, the 11-1 strain yielded the highest laccase activity up to 3120.0 ± 170 mU/ml (1.2 U/mg protein) within 5 days. A laccase gene (LAC1) of the yeast strain 11-1 contained two introns and encoded a protein with 570 amino acids and four conserved copper-binding domains typical of the fungal laccase. Expression of the LAC1 gene in the yeast strain 11-1 made a recombinant yeast strain produce the laccase activity of 6005 ± 140 mU/ml...
November 19, 2018: Marine Biotechnology
Ping Wu, Wuying Chu, Xuanming Liu, Xinhong Guo, Jianshe Zhang
Circadian clock genes and myogenic factors are tightly integrated to influence muscle growth upon dietary deprivation in animals. In this study, we reported that upon short-term fasting of Nile tilapia juveniles for 7 and 15 days, the growth of the fish stagnated and the size of muscle fibers decreased. To reveal the molecular mechanisms of how starvation affects fish muscle growth, we analyzed the rhythmic expression of circadian clock genes and myogenic factors. After 7 and 15 days of fasting treatment, the muscle tissues were collected for 24 h (at zeitgeber times ZT0, ZT3, ZT6, ZT9, ZT12, ZT18, ZT21, and ZT24) from tilapia juveniles...
December 2018: Marine Biotechnology
Jisun Lee, Seul Lee, Andriy Synytsya, Peter Capek, Chang Won Lee, Ji Won Choi, Sarang Cho, Woo Jung Kim, Yong Il Park
Low molecular weight mannogalactofucans (LMMGFs) prepared by enzymatic degradation of high molecular weight Undaria galactofucan (MF) were evaluated for their anti-cancer effects against human prostate cancer. Correlation NMR and linkage analyses confirmed that LMMGFs consist mainly of α-fucose and β-galactose units: α-fucose units are 1,3-linked; β-galactose units are terminal, 1,3- and/or 1,6-linked; both sugars are partially sulphated, fucose at positions O-2 and/or O-4 and galactose at O-3. Mannose residue, as a minor sugar, presents as the 1,4-linked terminal units...
December 2018: Marine Biotechnology
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