Journals Applied Immunohistochemistry &...

Applied Immunohistochemistry & Molecular Morphology : AIMM
Hanaa M Ibrahim, Aziza E Abdelrahman, Amira Elwan, Adel Bakry, Moamna M Fahmy, Mohamed I Abdelhamid, Abdelfatah H Abdelwanis, Enas M Fouad
There is a cellular crosstalk between Wnt/β-catenin and Hippo/Yes-related protein 1 signaling paths in colon cancer (CC) which promotes EMT processes that mediate the metastatic progression of CC. We aimed to evaluate follistatin-like 3 (FSTL3), ADAM12, and FAT4 expressions in CC. A statistical analysis was done to establish how disease-free survival, overall survival (OS), and relapse all performed a prognostic role. High FSTL3 was detected in 68% of CC and significantly related to left-sided tumors (P = 0...
September 26, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Lin Xu, Guohua Yu, Lei Jiang, Xiao Song, Guimei Qu, Jie Luo, Li Cai
OBJECTIVE: To investigate the expression and clinical significance of HepPar1 and GATA-3 in neuroendocrine neoplasms (NENs). MATERIALS AND METHODS: The expression of HepPar1 and GATA-3 in 144 cases of NENs was detected using immunohistochemistry, and the relevant literature was reviewed. RESULTS: HepPar1 was localized in the cytoplasm, and the positive rate of HepPar1 was 6.25% (9/144) in 144 NENs, 9 of which were derived from gastrointestinal and pancreatic NENs, including 4 cases of neuroendocrine tumor, grade 1 (NET G1), 4 cases of NET G2, and 1 case of NET G3...
September 26, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Aysel Bayram, Sidar Bagbudar, Hamdullah Sozen, Semen Onder, Ekrem Yavuz
Hereditary leiomyomatosis and renal cell carcinoma is caused by germline mutations in the fumarate hydratase (FH) gene and is associated with an increased incidence of leiomyomas and a potentially aggressive variant of renal cell carcinoma. Pathologic evaluation of uterine leiomyoma can provide an opportunity for early recognition of the syndrome. We reviewed all archived slides of the cases to identify the characteristic morphologic features described for FH-deficient leiomyomas. We performed immunohistochemistry on whole sections of patients with uterine leiomyoma to evaluate for both FH and 2-succinocysteine (2SC) expression...
September 25, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Serenella Serinelli, Brent A Enniss, Timothy A Damron, Harlan Stock, Gustavo L de la Roza
Nonossifying fibroma (NOF) is a common benign bone neoplasm and is usually observed in the first 2 decades of life. Most NOFs occur in the metaphysis of long bones of the lower extremities and migrate toward the diaphysis during skeletal maturation. Epiphyseal involvement by NOF has been rarely reported, with only one case found in the English literature. The authors report the second case of NOF involving the epiphysis of a long bone, the proximal tibia of a 21-year-old woman. Clinicians and pathologists should be aware of the rare possibility of epiphyseal involvement of long bones by this condition...
September 25, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Ana M Colino Gallardo, Montserrat De la Torre Serrano, Lorenzo Alarcón García, Isabel Casado Fariñas, David De Pablo Velasco, Javier Martínez-Useros, Rodrigo Barderas, María J Fernández-Aceñero
Corneal dystrophies are hereditary diseases affecting the corneal tissue; they are bilateral, symmetrical and unrelated to environmental or systemic conditions. Congenital corneal stromal dystrophy is a very rare autosomal dominant dystrophy that is caused by a mutation in the DCN gene that encodes decorin (a proteoglycan of the extracellular matrix). We herein report 4 cases of congenital stromal corneal dystrophy in 2 families, highlighting the previously undescribed histopathologic features, the possible differential diagnosis of this entity and the key role played by decorin staining in its diagnosis...
September 20, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Gwyneth S T Soon, Saba Yasir, Dhanpat Jain, Sanjay Kakar, Tsung-Teh Wu, Matthew M Yeh, Michael S Torbenson, Zongming Eric Chen
Subtyping hepatic adenomas is important for patient management due to differing complication risks. Immunohistochemical staining with C-reactive protein (CRP) and serum amyloid-A (SAA) is widely accepted as a surrogate for molecular classification to identify inflammatory hepatocellular adenomas. Limited data, however, has been published on how these 2 stains compare for sensitivity. We conducted a large, multicenter, retrospective study to examine the sensitivity and staining characteristics of CRP and SAA in inflammatory hepatic adenomas, with focal nodular hyperplasia (FNHs) as a control group...
September 13, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Wiebke Solass, Giorgi Nadiradze, Marc A Reymond, Hans Bösmüller
INTRODUCTION: The Peritoneal Regression Grading Score (PRGS) is a 4-tied histologic regression grading score for determining the response of peritoneal metastasis to chemotherapy. Peritoneal biopsies in every abdominal quadrant are recommended. A positive therapy response is defined as a decreasing or stable mean PRGS between 2 therapy cycles. The added value of periodic acid satin (PAS) and Ber-EP4 staining over HE staining for diagnosing PRGS1 (the absence of vital tumor cells) is unclear...
September 13, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Heba A S Bazid, Hanaa G Sakr, Rania A Abdallah, Eman S Arafat, Ahmed Ragheb, Iman Seleit
BACKGROUND: Uremic pruritus is an irritating symptom for patients with end-stage kidney disease. Lipocalin-2 (LCN2) has relevant importance in several biological cellular processes and immunity. It is also a major player in the progression of many disorders, such as renal injury. AIM: To evaluate LCN2 expression in chronic kidney disease (CKD) pruritic patients in serum together with immunohistochemical expression in skin samples and further correlation of their results with the studied clinicopathologic parameters...
September 13, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Susanne K Jeffus, Nikhil Meena, Matthew Lindberg
Tumors of the lung with a spindle cell morphology require consideration of many entities in the differential diagnosis, including metastases. Ancillary immunohistochemical stains but also molecular studies are typically required to arrive at the proper diagnosis. We present a case of a 71-year-old woman with multiple lung nodules, mediastinal lymphadenopathy, and a history of uterine cancer who underwent endobronchial ultrasound-guided fine needle aspiration and biopsy of the lung and mediastinal lymph nodes...
September 6, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Angels Barberà, Juan González, Montserrat Martin, Jose L Mate, Albert Oriol, Fina Martínez-Soler, Tomas Santalucia, Pedro Luis Fernández
Antibodies targeting programmed death receptor 1 or programmed death ligand 1 (PD-L1) have become a standard of care to treat different cancers; for some of these tumors, there is a correlation between tissue expression of PD-L1 and response rates in patients. Although most of the analytical challenges in the evaluation of PD-L1 expression have been standardized, preanalytical issues have been less explored. The objective of this study was to evaluate the impact of time of ischemia on the performance of 2 commonly used antibodies against PD-L1...
September 5, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Aiat Shaban Hemida, Doha Maher Taie, Moshira Mohamed Abd El-Wahed, Mohammed Ibrahim Shabaan, Mona Saeed Tantawy, Nermine Ahmed Ehsan
Liver transplantation (LT) is a good therapeutic decision, cures hepatocellular carcinoma (HCC) and promotes survival of cases with unrespectable HCC based on the Milan criteria. HCC still recur after LT. Identifying high risk tissue markers that predict recurrence becomes important for LT decision-making. Little is known regarding use of tissue expression of epithelial cell adhesion molecule (EpCAM) to predict HCC recurrence. This study investigates the role of EpCAM, Ki67, and endothelial-cell-specific molecule-1 (ESM1) as immunohistochemical markers to predict HCC recurrence after LT...
September 4, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Dalia M Badary, Hisham A Abou-Taleb, Maha Ibrahim
BACKGROUND AND STUDY AIM: We aim to study the immunohistochemical expression of both hypoxia-inducible factor-1α (HIF-1α) and mammalian target of rapamycin (mTOR) in endometriosis to provide new evidence for a targeted endometriosis therapy. PATIENTS AND METHODS: This study comprised 106 endometriotic cases diagnosed clinically and histopathologically. The immunohistochemical method was done to determine the expression of HIF-1α and mTOR. RESULTS: Endometriotic glands showed significant cytoplasmic expression of both markers in patients with poor ovulation, severe endometriosis, and infertile for >2 years (P<0...
August 30, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Rana Aldrees, Gene P Siegal, Shi Wei
Compelling data has demonstrated the prognostic significance of tumor-infiltrating lymphocytes (TILs) in triple-negative breast cancer (TNBC), a subtype generally associated with a poor clinical outcome but highly heterogeneous in nature. There have been limited studies investigating the importance of subsets of T cells in TILs. Further, the significance of intratumoral versus peritumoral TILs remains controversial. We examined the prognostic value of tumor-associated CD8+ cytotoxic T cells and FOXP3+ regulatory T cells in 35 chemotherapy-naive TNBC cases with a tumor-host interface in the tissue sections...
August 25, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Sufia Husain
Primary focal segmental glomerulosclerosis (FSGS) is a type of chronic renal disease that commonly progresses to renal failure as the treatments are not particularly effective. Glomerular podocyte injury and loss are pivotal to the pathogenesis of FSGS. This study aims to explore the glomerular immunohistochemistry stain expression of Wilms tumor-1 (WT-1) (podocyte-specific protein), transforming growth factor beta (TGF-β) (cytokine protein), vascular endothelial growth factor (VEGF) (angiogenic protein), and endothelin-1 (ET-1) (profibrotic growth factor), in rats with adriamycin nephropathy, which represents the murine model of human FSGS...
August 3, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Pierre-Olivier Fiset, Gilbert Bigras
No abstract text is available yet for this article.
July 27, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Andrew M Bellizzi
This review is based on a webinar I presented for the International Society for Immunohistochemistry and Molecular Morphology (ISIMM) in February 2022. It is intended that all ISIMM webinars will ultimately be published in AIMM as review articles. This work is also dedicated to Clive Taylor, who has deeply impacted my career. It presents a molecularly informed, pattern-based approach to p53 immunohistochemistry interpretation, methodological considerations (ie, antibody selection, optimization, validation, controls, and external quality assessment), and pan-cancer diagnostic applications, including those drawn from gastrointestinal, genitourinary, gynecological, neuroendocrine, hematopathology, and neuropathology...
July 21, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Dheeraj Chinnam, Aastha Saraswati, Swathi Jogunoori, Aanchal Verma, Tanvi Kiran, Pravin Salunke, Nalini Gupta, Narendra Kumar, Renu Madan, Bishan Dass Radotra, Kirti Gupta
BACKGROUND: The WHO classification of central nervous system neoplasms (2016) recognized 4 histologic variants and genetically defined molecular subgroups within medulloblastoma (MB). Further, in the 2021 classification, new subtypes have been provisionally added within the existing subgroups reflecting the biological diversity. YAP1, GAB1, and β-catenin were conventionally accepted as surrogate markers to identify these genetic subgroups. OBJECTIVES: We aimed to stratify MB into molecular subgroups using 3 immunohistochemical markers...
July 21, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Ezra G Baraban, Yasser Ged, Nirmish Singla, Mohammad E Allaf, Michael A Gorin, Mark C Markowski, Steven P Rowe, Pedram Argani
Multiple studies have demonstrated prostate-specific membrane antigen (PSMA) expression in the neo-vasculature of non-prostate tumors including clear cell renal cell carcinoma (ccRCC). However, PSMA expression in rare renal tumors including MiTF family translocation renal cell carcinoma has not been previously characterized. We examined PSMA expression by immunohistochemistry in a series of MiTF family translocation renal cell carcinomas as well as in several genetically related tumors including alveolar soft part sarcoma and PEComas with TFE3 rearrangements...
July 20, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Reena Dhansukh Mohanlal, Nikki Bouwer, Pascale Willem
The American Society of Clinical Oncology and the College of American Pathologists (ASCO/CAP) guidelines are used for human epidermal growth factor receptor 2 (HER2) reporting in breast carcinoma. Cases that demonstrate weak to moderate complete membrane immunohistochemical staining in >10% of the tumor are scored as 2+ (equivocal). This study aimed to determine what proportion of HER2 immunohistochemistry (IHC) score = 2+ breast carcinomas were confirmed to be positive by HER2 fluorescent in situ hybridization (FISH)...
July 6, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
Lixin Wang, Gang Chen, Chen Zhou, Chao Wu, Jingui Jiang
The aim of this study was to investigate the expression and clinical significance of MTA2 and CPNE1 proteins in cervical squamous cell carcinoma. In this study, high-risk human papillomavirus (HPV) typing was performed on cervical cancer tissues. Reverse transcription polymerase chain reaction and immunochemical EliVision method were used to examine the expressions of MTA2 and CPNE1 in the cervix, and their relationship with clinicopathologic features. We found that it is mainly distributed in these types, namely HPV-16 (23...
July 3, 2023: Applied Immunohistochemistry & Molecular Morphology: AIMM
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