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Applied Immunohistochemistry & Molecular Morphology: AIMM

Najat Mourra, Ismail Matalka, Lionel Arrive
No abstract text is available yet for this article.
February 11, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Naziheh Assarzadegan, David H Gonzalo, Marino E Leon, Jaya R Asirvatham
No abstract text is available yet for this article.
February 6, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Rachel Miller, Trish Thorne-Nuzzo, Isabell Loftin, Abigail McElhinny, Penny Towne, June Clements
Diagnostic assays for molecular alterations highly correlated with prognosis, predictive efficacy or safety of therapeutics are valuable clinical tools and in some cases approved as companion diagnostics (CDx) by the Federal Food and Drug Administration. For example, assays that determine echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) translocation status have been approved as CDx assay for therapies that target this molecular alteration. Characterizing the parameters that may compromise diagnostic accuracy for molecular biomarkers is critical for optimal patient care...
February 4, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Xingang Zhou, Peng Wang, Zhiyuan Ma, Man Li, Xiaoying Teng, Lei Sun, Gang Wan, Yang Li, Limei Guo, Honggang Liu
Nonalcoholic steatohepatitis (NASH) has the potential to progress to fibrosis, cirrhosis, and hepatocellular carcinoma. Upregulation of sonic hedgehog (Shh) has been documented in development of NASH through sustained cell stress. At the same time, transforming growth factor-β1 (TGF-β1), which is a central element in fibrogenic reactions in various diseases and sites, has been reported to be associated with hepatic inflammation and fibrotic reaction. To explore crosstalk between Shh and TGF-β1 in the development and progression of NASH, we investigated the expression of both these proteins in 135 human specimens of NASH, 35 fatty liver specimens, 35 specimens of alcoholic steatohepatitis with immunohistochemistry...
February 1, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Paul E Swanson
No abstract text is available yet for this article.
February 1, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Xiaoyan Wang, Shaoxiong Chen, Robert E Emerson, Howard H Wu, Harvey M Cramer, Kendra Curless, Hsim Yee Chang, Shaobo Zhang, Melissa L Randolph, Liang Cheng
OBJECTIVE: The aim of this study was to investigate epidermal growth factor receptor (EGFR) gene mutations and anaplastic lymphoma kinase (ALK) gene rearrangements using cytological specimens from the patients with a diagnosis of primary or metastatic lung non-small cell carcinoma. MATERIALS AND METHODS: A total 307 cases were submitted for EGFR mutational analysis and 265 cases for ALK analysis. The cytological specimen sources included lung, lymph node, liver, bone, adrenal gland, mesentery mass, and body fluids/bronchial brushing...
January 25, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Xiaoqi Lin, Sydney D Finkelstein, Jan F Silverman
Many genomic mutations have been identified to be related to the metastasis of malignancies from various primary sites. In this study, we attempted to identify the loss of heterozygosity (LOH) that might be involved in metastasis of breast ductal carcinoma (BDC) and papillary thyroid carcinoma (PTC). We retrieved 14 BDC cases with metastasis and 19 BDC cases without metastasis as well as 12 PTC cases with metastasis and 14 PTC cases without metastasis. Analysis of 13 polymorphic microsatellite repeat markers targeting 1p34-36, 3p24-26, 9p21, 10q23, 17p13, 17q21, 21q22, and 22q13 was performed on DNA isolated from primary tumors...
January 25, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Christopher A Febres-Aldana, John Alexis
Desmoplastic malignant melanoma (DMM) is an amelanotic spindle cell proliferation that can be mistaken for a cutaneous scar. The distinction can be difficult in reexcisions because DMM is negative for conventional melanoma markers such as HMB-45 and Melan-A, and scars may be positive for S-100 protein and SOX-10. We compare a total of 12 DMM cases with 8 reexcision and 35 old non-reexcision cutaneous scars using SOX-10 immunohistochemical stains. Cell quantification was performed on captured images using ImageJ 1...
January 21, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Jon Griffin, Fawzia Tahir
We present a case of synchronous renal cell carcinoma and urothelial carcinoma. Our case was a true collision tumor and both components were high grade. Metastasis to the adrenal gland by the urothelial carcinoma was observed. The morphologic impression was confirmed with GATA3 expression by the urothelial carcinoma component and PAX8 expression by the renal cell carcinoma component. We have reviewed the contemporary literature of this unusual dual diagnosis.
January 21, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Zheng-Yi Huang, Dueng-Yuan Hueng, Wen-Chiuan Tsai
Forkhead box, class O, 1A (FOXO1A) is an important factor related to some human malignancies. We tested the association between FOXO1A cytoplasmic expression and World Health Organization grading system in primary brain tumors (PBTs). First of all, Western blot analysis was also performed in normal brain tissue and U87MG, LN229, GBM8401, and U118MG glioma cell lines protein lysates. Then, in order to realize FOXO1A gene expression in gliomas, U87MG, LN229, GBM8401 mRNA were applied to performed quantitative reverse transcription polymerase chain reaction (RT-PCR)...
January 19, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Chad M Ennis, Marc R Rohrbach, Michael Schwalbe, Aparna Mahajan, Gregory K Hartig
The purpose of this investigation is to directly compare G175-405 and E6H4 p16-specific antibodies as immunomarkers of HPV-driven oropharyngeal carcinoma. The investigators designed a retrospective analysis using specimens from an archived tissue bank with known in situ hybridization and polymerase chain reaction status for HPV DNA. Fifty randomly selected oropharyngeal specimens were evaluated with both the G175-405 and E6H4 p16-specific monoclonal antibodies. Two pathologists, blinded to the HPV-specific testing status, evaluated p16 positivity for both antibody clones...
January 19, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Omer Saeed, Shanxiang Zhang, Liang Cheng, Jingmei Lin, Fatimah Alruwaii, Shaoxiong Chen
STAT6 stain has proved to be a good surrogate marker for the genetic alteration (NAB2-STAT6 gene fusion) in solitary fibrous tumor (SFT). This study aims to validate the use of STAT6 rabbit monoclonal antibody in differentiating SFT from its histologic mimics. Forty-five cases of SFT and 110 cases from 9 other spindle cell tumors were collected for STAT6 immunostaining. Positive nuclear STAT6 staining was present in all 45 SFT cases (100% sensitivity). No nuclear staining was identified in other spindle cell neoplasms (0/13, dedifferentiated liposarcoma; 0/17, synovial sarcoma; 0/16, malignant peripheral nerve sheath tumors; 0/25, undifferentiated pleomorphic sarcoma; 0/10, dermatofibrosarcoma protuberans; 0/9, low-grade fibromyxoid sarcoma; 0/6, angiofibroma; 0/5, deep fibromatosis; 0/9, gastrointestinal tumor)...
January 19, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Mahra Nourbakhsh, Parham Minoo
There is increasing body of evidence to suggest that some colonic serrated polyps do not fit morphologically with any of the proposed categories for serrated polyps recommended by the World Health Organization. Most of these polyps have morphologic features of traditional serrated adenoma (TSA) admixed with areas resembling sessile serrated adenoma (SSA) or hyperplastic polyp (HP). Based on these findings it has been suggested that at least some TSAs may arise in association with precursor HP or SSA lesions, particularly those that develop in right colon...
January 15, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Rasmus Røge, Heidi L Kristoffersen, Michael Bzorek, Ole Nielsen, Mogens Vyberg
This paper is number 6 in a series developed through a partnership between ISIMM and Nordic IHC Quality Control with the purpose of reporting research assessing the performance characteristics of immunoassays in an external proficiency testing program.
January 15, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Chih-Hung Lin, Chien-Hui Wen, Chia-Hsing Liu, Ching-Hsiu Yang
CONTEXT: Human epidermal growth factor receptor 2 (HER2) status of breast carcinomas is usually determined by immunohistochemical (IHC) staining and, if the IHC results are equivocal, in situ hybridization (ISH). Multiple ISH tests are sometimes required for multiple primary or metastatic tumors. A method for multiplex ISH test on tissues from multiple blocks is helpful in these situations. OBJECT: To evaluate the clinical application of transferred-tissue microarray (TTM) followed by a dual-probe HER2 fluorescence in situ hybridization (FISH)...
January 15, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Chih-Ying Wu, Ren-Ching Wang, Bo-Jung Chen, Wei-Yu Chen, Jie-Yang Jhuang, Ming-Chih Chang, Yu-Hung Wu, Norihiro Nakada, Kennosuke Karube, Shih-Sung Chuang
Granulomatous reaction is not uncommon in histopathology, with various etiologies in different organs and geographic regions. Lymphoma is one of the underlying causes of granuloma; and sometimes the neoplastic cells may be masked by the granulomatous reaction. In this report, we present our experience with 7 lymphoma cases of various histologic types with coexisting granuloma to show the diagnostic challenges. In all cases, a granulomatous reaction was simultaneously present with the neoplastic cells. The 7 cases included 3 cases of adult T-cell leukemia/lymphoma in the lymph node or skin including one coexisting with mycobacterial infection, 2 cases of classical Hodgkin lymphoma involving the liver, and 1 case each of systemic Epstein-Barr virus-positive peripheral T-cell lymphoma and a hepatic inflammatory pseudotumor-like follicular dendritic cell sarcoma...
January 15, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Ramona Erber, Arne Warth, Thomas Muley, Arndt Hartmann, Esther Herpel, Abbas Agaimy
Malignant mesothelioma (MM) can show areas closely mimicking reactive mesothelial proliferations or recapitulating benign adenomatoid tumors (ATs) making distinction on occasion impossible on morphologic ground alone, particularly in limited biopsy material. Recently, loss of BAP1 by immunohistochemistry (IHC) has been suggested as a potential marker for identifying MM, but data is still limited. We studied 264 MM cases (257 using tissue microarrays; 7 on conventional slides) and 42 genital ATs for BAP1 immunohistochemical expression...
January 11, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Clive R Taylor, Anagha P Jadhav, Abhi Gholap, Gurunath Kamble, Jiaoti Huang, Allen Gown, Isha Doshi, David L Rimm
Assessment of programmed death-ligand 1 (PD-L1) expression is a critical part of patient management for immunotherapy. However, studies have shown that pathologist-based analysis lacks reproducibility, especially for immune cell expression. The purpose of this study was to validate reproducibility of the automated machine-based Optra image analysis for PD-L1 immunohistochemistry for both tumor cells (TCs) and immune cells. We compared conventional pathologists' scores for both tumor and immune cell positivity separately using 22c3 antibody on the Dako Link 48 platform for PD-L1 expression in non-small cell lung carcinoma...
January 11, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Vidhya Manohar, Raheem Peerani, Brent Tan, Dita Gratzinger, Yasodha Natkunam
Myeloid cell nuclear differentiation antigen (MNDA) is an immunohistochemical marker that is used to distinguish marginal zone lymphomas (MZLs) from other small B-cell lymphomas. An index case that showed MNDA staining in primary follicles prompted the current study to evaluate whether MNDA expression is widespread in primary follicles and to address whether it poses a potential diagnostic pitfall. Of the 15 cases with primary follicles identified by a search of the laboratory information system, 7 had positive MNDA staining...
January 11, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
Michael Bonert, Ihab El-Shinnawy, Mozibur Rahman, Pierre Major, Samih Salama, Bobby Shayegan, Jean-Claude Cutz, Anil Kapoor
BACKGROUND: Immunohistochemistry (IHC) use in prostate cores is not routinely determined and its value assessed. METHODS: Pathology reports for cases accessioned 2011 to 2017 at two hospitals were retrieved. IHC orders by pathologist and hospital were extracted with a custom program and tabulated. The diagnostic category (and highest grade cancer if applicable) was obtained by a hierarchical (free text) string matching algorithm. RESULTS: The study period contained 4477 biopsy sets...
January 8, 2019: Applied Immunohistochemistry & Molecular Morphology: AIMM
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