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British Poultry Science

Yuxiang Wang, Haixia Wang, Wei Na, Feiyue Qin, Zhiwei Zhang, Jiaqiang Dong, Hui Li, Hui Zhang
1. The objective of this study was to reveal the role of chicken RB1 (Gallus gallus RB1, gRB1) in the proliferation of preadipocytes. 2. To measure gene expression of gRB1 in the proliferation of chicken preadipocyte, quantitative real-time PCR was used. The expression levels of gRB1 transiently increased during this process. 3. To detect the effect of gRB1 on the proliferation of chicken preadipocyte, MTT assay and cell-cycle analysis were performed. MTT assay showed that overexpression of gRB1 significantly suppressed (P<0...
February 20, 2019: British Poultry Science
B Hughes, C Whitehead
No abstract text is available yet for this article.
February 6, 2019: British Poultry Science
Yingxiao Fu, Yihui Wang, Xishuai Tong, Zheng Gong, Xuemin Sun, Jianchang Yuan, Tiantian Zheng, Chuang Li, Dequn Niu, Honggang Dai, Xianfu Liu, Yingji Mao, Baoding Tang, Wei Xue, Yinjiu Huang
This study determined the effects of (E)-3-(2-(4-(3-(2,4-dimethoxyphenyl)acryloyl)phenoxy)ethoxy)-5,7-dimethoxy-2-(3,4,5-trimethoxyphenyl)-4H-chromen-4-one (EDACO) on the differentiation of Gaoyou duck embryonic osteoclasts cultured in vitro. Bone marrow mononuclear cells (BM-MNC) were collected from 23-day-old Gaoyou duck embryos and induced by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL) in the presence of EDACO at different concentrations (i.e., 10, 20, 40, 80, and 160 µM)...
February 5, 2019: British Poultry Science
T K Bhattacharya, R N Chatterjee, Minakshi Dange, S K Bhanja
1. Two candidate genes, namely, Gonadotropin releasing hormone I (GnRHI) and Gonadotropin releasing hormone II (GnRHII) play pivotal roles in ovulation and egg production in chicken. The objective of this study was to explore polymorphism in these genes and to estimate the effects of polymorphism of these two genes on egg production and egg quality traits in White Leghorn layer chicken. 2. Single strand conformation polymorphism followed by sequencing was performed to detect polymorphism in these genes. 3. The coding regions of the GnRHI and GnRHII genes were found to be polymorphic...
January 28, 2019: British Poultry Science
Amin Kazemizadeh, Ahmad Zare Shahneh, Saeed Zeinoaldini, Ali Reza Yousefi, Hassan Mehrabani Yeganeh, Zarbakht Ansari Pirsaraei, Amir Akhlaghi
1. Decreased semen quality is an underlying contributor to age-related subfertility in broiler breeder roosters. This study investigated the effects of dietary curcumin (derived from turmeric) supplementation as an antioxidant source on semen quality and fertility in broiler breeder roosters. 2. Twenty-eight Ross 308 roosters were randomly allotted to four groups with seven birds in each and were fed a standard diet supplemented with different levels of curcumin at 0 (C0 ), 10 (C10 ), 20 (C20 ), and 30 (C30 ) mg/bird per day from 48 through to 61 weeks of age...
January 22, 2019: British Poultry Science
Dong XiaoFang, Tong Jian Ming
1. The objective of the study was to investigate the susceptibility of young and older laying hens to fatty liver-haemorrhagic syndrome (FLHS) and to evaluate the reliability of different blood lipid fractions for predicting or diagnosing FLHS. 2. Forty young hens and 40 older hens were caged individually. Each group of hens was randomly allotted to four treatments for 21 days: either a control, an oestradiol group, high energy-low protein diet (HELPD) or a HELPD + oestradiol group. Blood levels of oestradiol, triglyceride (TG), cholesterol (CHOL), high density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C), liver total lipids, haemorrhagic scores and productive performance were assessed...
January 18, 2019: British Poultry Science
Ding-Ping Bai, Yu-Qiong Hu, Yi-Bin Li, Zhong-Bin Huang, Ang Li
Prolactin (PRL) as a polypeptide hormone which plays a crucial role in egg production traits. Polymorphisms of the PRL gene were analysed with DNA sequencing and polymerase chain reaction-single-strand conformation polymorphism methods in two Chinese domestic laying duck breeds (Jinding n=400, Youxian n=400, respectively). The results showed that one polymorphism was detected (A-412G) in intron 1 of the PRL gene, with three genotypes: AA, AG, and GG. Association analysis showed that the ducks with the GG genotype had significantly greater egg production and egg weight than those with AG and AA genotype (P<0...
January 16, 2019: British Poultry Science
Y J Polizer Rocha, J M Lorenzo, J C Barros, J C Baldin, M A Trindade
1. Sausages were used to assess the effects of chicken meat replacement by spent laying hen meat on the quality parameters and sensorial properties. 2. Five formulations were developed: control (CON) containing 100% chicken meat and four levels of chicken meat replacement with spent laying hen meat: 25% (T25), 50% (T50), 75% (T75) and 100% (T100). 3. Appearance, texture, succulence, flavour and overall consumer acceptance were analysed. Parameters such as moisture, ash, fat and protein content, pH, cooking loss and colour parameters (L*, a*, b*) were analysed, as well as the texture properties (hardness, elasticity, cohesiveness and chewiness)...
January 16, 2019: British Poultry Science
E Aka, U Eren
The purpose of this study was to investigate the distribution of TLR4 and MHC class II molecules of the spleen in chicks treated with LPS (lipopolysaccharide) during the first two weeks of their life. A total of 225 Ross-308 commercial broiler chicks were used. Within the two-week experimental period, chicks were divided into five main groups according to the days of decapitation which were 1, 4, 7, 10, and 14 days after hatch. Each main group had 45 chicks. The main groups were further divided into three subgroups (15 chicks each), which included control chicks (no injection), and phosphate-buffered saline and lipopolysaccharide-injected chicks...
January 16, 2019: British Poultry Science
F Shao, X Wang, J Yu, K Shen, C Qi, Z Gu
1. Limiting the growth of adipose tissue in chickens is a major issue in the poultry industry. In chickens, de novo synthesis of lipids occurs primarily in the liver. Thus, it is necessary to understand how fatty acid accumulation in the liver is controlled. The miR-33 is an intronic microRNA (miRNA) of the chicken sterol regulatory element binding transcription factor 2 (SREBF2), which is a master switch in activating many genes involved in the uptake and synthesis of cholesterol, triglycerides, fatty acids and phospholipids...
January 4, 2019: British Poultry Science
Tao Huang, Jun Ma, Yanzhang Gong, Yanping Feng
Chicken ovoinhibitor has been reported to prevent contamination and affect storage for eggs. The objective of the present study was to conduct an association analysis between ovoinhibitor gene (OIH) polymorphisms and egg quality traits in the population of Xinhua E-strain chickens and explore its expression characteristics in different tissues. Three single nucleotide polymorphisms (SNPs) were identified and genotyped, in which one synonymous mutation was located in exon16 (G9810A), one in intron7 (A4363G) and one in intron14 (C8937G)...
January 4, 2019: British Poultry Science
Shu-Wen Tan, Pei-Zhou Li, Hua Li, Hui Yu, Zhang Zheng-Fen, Zheng Zeng, De-Chun Huang
1. The goal of the current study was to evaluate the genetic effects of the vascular endothelial growth factor (VEGF) and its receptor (VEGFR-2) on feather maturity in the Qingyuan partridge chicken, Guangxi Sanjuana chicken and Princesss chicken. 2. Both SSCP-PCR and qPCR were employed to detect the polymorphism and gene expression of the VEGF and VEGFR-2 genes. 3. Four SNPs were identified in the VEGFR-2 gene. Exon10-A69G was associated with feather maturity (P<0.01). Princesss chickens with the genotype EF had higher feather maturity scores (P<0...
January 3, 2019: British Poultry Science
Guo-Qin Li, Yong Tian, Li Chen, Jun-da Shen, Zheng-Rong Tao, Tao Zeng, Jian Xu, Li-Zhi Lu
1. Melanoma differentiation-associated gene 5 (MDA5) is a critical member of cytosolic pattern recognition receptors (PRRs) that recognise viral RNA and mediate type I interferon secretion in host cells. 2. The objective of the present study was to identify and characterise the structure and expression of pigeon MDA5. 3. The full-length MDA5 cDNA was cloned from pigeon spleen using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The distribution and expression level of pigeon MDA5 in different tissues were determined by quantitative real-time PCR (QRT-PCR)...
December 30, 2018: British Poultry Science
Michał Gesek, Daria Murawska, Iwona Otrocka-Domagała, Katarzyna Paździor-Czapula, Korina Michalska
Poultry researchers seek the most efficient bird line to produce capons (castrated cockerels). The previous studies did not include results from histopathological analyses of internal organs after caponisation. No data have been published on whether adipose tissue accumulates in internal organs or if caponisation changes the structure of the lymphoid organs and the accumulation of lymphoid cells. The aim of this study was to analyse the occurrence of histopathological lesions in internal organs from Leghorn (layer-type) capons and cockerels at different times of fattening...
December 30, 2018: British Poultry Science
M R Ebadi, M Sedghi, R Akbari Moghaddam Kakhki
1. This study evaluated the application of L (lightness)*a (redness) and *b (blueness) colour analysis and chemical compositions to predict the nutritional value of sorghum grain. 2. A total of 12 varieties of sorghum grain were analysed for L*a*b colours, chemical composition, energy and total and digestible amino acid content. Regression models based on the linear, non-linear and the interaction effects of inputs were applied to predict the nutritional value of sorghum grains either using L*a*b colour or chemical composition, as the model inputs...
December 30, 2018: British Poultry Science
S S Ale Saheb Fosoul, A Azarfar, A Gheisari, H Khosravinia
1. The present experiment was designed to examine arginine (Arg)-sparing effects of guanidinoacetic acid (GAA) on production performance, intestinal morphology and certain blood parameters in broiler chickens. 2. A total of 300 male broiler chicks (Ross 308) were randomly allotted to 5 dietary treatments in a completely randomised design during 1-15 and 15-35 d rearing periods. Experimental treatments consisted of a basal diet with a reduction in Arg content (CON-; starter: 12.1 g/kg and grower: 11.3 g/kg Arg), a CON- diet supplemented with synthetic Arg equal to the required Arg level (CON+; starter: 13...
December 30, 2018: British Poultry Science
Liubin Yang, Changhuan Mo, Wei Shen, Xiaoyong Du, Ali Akbar Bhuiyan, Ling Li, Na Li, Yanzhang Gong, Shijun Li
1. The c/c alleles are responsible for the white plumage colour of ducks; however, the gene corresponding to this locus is still unclear. In order to identify the locus related candidate gene associated with duck's plumage colour pattern, it was necessary to analyse the whole genome resequencing data. 2. A total of 929,465 SNPs in chromosome 13 and 1,688 SNPs in the region of the Microphthalmia Associated Transcription Factor (MITF) gene were identified from whole genome resequencing data analysis. After construction of an FST plot from chromosome 13, MITF was highlighted as a candidate gene, possessing the highest FST value (0...
December 30, 2018: British Poultry Science
Tobias Zuber, Wolfgang Siegert, Hamed Salehi, Franziska Hummel, Markus Rodehutscord
1 The objective of this study was to investigate the variations in amino acid (AA) digestibility of lupin and pea grains in caecectomised laying hens. The relationship between AA digestibility and chemical constituents of the grains was determined. 2. Twelve variants of lupins and peas were each added to a basal diet at a concentration of 300 g/kg, at the expense of maize starch. The lupin and pea variants were separately investigated in two subsequent trials. A run consisted of two 7×7 Latin squares, each comprising the basal diet and six diets with lupins or peas...
December 12, 2018: British Poultry Science
L A Westbrook, G Cherian
Flaxseed is a rich source of α-linolenic acid (ALA, 18:3 n-3). Feeding flaxseed to hens can increase n-3 fatty acids (FA) in eggs. However, non-starch polysaccharides (NSP) in flaxseed decrease nutrient digestibility and can have a negative impact on egg n-3 FA incorporation. Addition of carbohydrase enzymes to flaxseed-based diets can decrease the anti-nutritive effects of NSP. An experiment was conducted to investigate the effect of enzyme supplementation on FA composition and gastrointestinal morphology in hens fed flaxseed...
December 10, 2018: British Poultry Science
Khaled Itani, Birger Svihus
1. A 2x2 factorial design was used to test the hypothesis that impaired intestinal starch digestibility is attributable to rapid passage of digesta from the gizzard to the intestine, and that, compared to steam pelleting, increasing the availability of starch through extrusion cooking may alleviate the potential negative effect of rapid digesta flow on starch utilisation. 2. Thus, 7-d-old broilers chickens were distributed to 48 cages and given a wheat-based (WB) pelleted diet containing either coarse oat hulls (OH-Pel) or fine cellulose (Cel-Pel) until d 19 to stimulate divergent development of the gizzard...
December 5, 2018: British Poultry Science
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