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Cryo Letters

L Wan, M J Powell-Palm, M G Clemens, B Rubinsky
BACKGROUND: Isochoric freezing systems enable ice-free preservation of biological matter at subfreezing temperatures under the increased hydrostatic pressure. OBJECTIVE: To examine the effects of pressure and exposure period on rat hearts preserved in an isochoric chamber. MATERIALS AND METHODS: Rat hearts were preserved in the UW solution in isochoric chambers at temperatures from -2°C to -8°C and pressure from the atmospheric level to 78 MPa for up to eight hours, with and without the addition of glycerol...
January 2019: Cryo Letters
G Wang, J Liu, J Luo, L Sheng
BACKGROUND: Ultrasound guidance of cryotherapy could be improved if changes of tissue characterization parameters during freezing could be used to monitor freezing profiles and estimate frostbite distributions. OBJECTIVE: To investigate if B-mode ultrasonic imaging can provide sufficient information to define the damaged zone during cryotherapy by quantifying the differences of texture features between fresh and frozen-thawed porcine liver tissue. MATERIALS AND METHODS: The B-mode ultrasonic images were obtained from ten porcine liver tissue samples before and after frozen-thawing treatment, then 20 texture parameters of the grey level histogram (GLH) and the grey level co-occurrence matrix (GLCM) from each image were extracted and analyzed statistically...
January 2019: Cryo Letters
A Angulo-Sherman
BACKGROUND: The use of isochoric containers below the freezing point was proposed for the reduction of freezing damage. OBJECTIVE: To determine mathematically the dielectric constant (k') of a sample inside an isochoric container depending on a suggested nucleated volume, and to compare the values with the reported k' for an isochoric container. METHODS: Different nucleation arrangements inside a cylindrical capacitor filled with water was considered, and the way that ice Ih changes the capacitance and the expected k' was examined...
January 2019: Cryo Letters
P B Rodríguez, A O Fernández, A Corral, M Balcerzyk, R Risco
BACKGROUND: In cryopreservation of biological material, it is very important to precise control the perfusion of cryoprotectants (CPA) inside the sample. CPA concentration inside tissues and organs during the process was measured in a few studies. The simulation of the CPA perfusion inside the organ is still necessary to understand and optimize this complex process. OBJECTIVE: This study simulates the diffusion of Me2 SO and PBS in a rabbit liver. MATERIALS AND METHODS: The software COMSOL for computational fluid-dynamics has been used...
January 2019: Cryo Letters
Y Acosta, L Hernandez, C Mazorra, N Quintana, B E Zevallos, I Cejas, EmptyYN Y Sershen, J C Lorenzo, M E Martínez-Montero, D Fontes
BACKGROUND: Teramnus labialis is an herbaceous legume that serves as a source of carbohydrates and proteins for animals and humans, and is valued for its nitrogen contribution to soil. The benefits of this species are, however, limited by low seed availability, small seed size and low in situ seed germination levels, due to physical dormancy. Cryostorage has been shown to be beneficial for both seed storage and breaking physical dormancy in seeds of various species. However, its potential effects on subsequent seedling emergence, plant growth and seed production need to be studied before large-scale implementation for T...
January 2019: Cryo Letters
D S Nair, B R Reghunath, K B Soni, S Alex
BACKGROUND: Clitoria ternatea is a brain revitalizing legume, with immense pharmacological value including antimicrobial, antioxidant and anticancer. The lack of a commercial cultivation has led to its' over collection from the wild to meet the demand of herbal pharmaceutical sector and the species is now rare in the wild. Hence, the plant needs to be conserved. Cryopreservation of the species would supplement the conventional conservation strategies in the field or seed bank. OBJECTIVE: The objective of the study was to evolve an efficient and simple protocol for the cryopreservation of Clitoria ternatea using an encapsulation-dehydration technique...
January 2019: Cryo Letters
S D Devi, S Kumaria, M C Das
BACKGROUND: Cryopreservation is the only possible process for long-term germplasm storage of species with recalcitrant behavior. Aquilaria malaccensis is a recalcitrant seeded tropical tree that produces a distinctive fragrance which has high value in the commercial market. OBJECTIVES: In the present study, we aimed to develop possible long-term storage techniques for A. malaccensis germplasm. MATERIALS AND METHODS: We used zygotic embryos and in vitro derived nodal buds as explants...
January 2019: Cryo Letters
Y Bozkurt, I Yavaş, M N Bucak, D Yeni
BACKGROUND: Cryopreservation of sperm from different fish species requires different protocols. Therefore, it is necessary to perform studies to establish reliable procedures for each species. OBJECTIVE: Experiments were designed to analyse the effect of different types of cryoprotectants on post-thaw motility, viability and fertility as well as cryoresistance of cryopreserved Nile tilapia (Oreochromis niloticus) sperm. MATERIALS AND METHODS: Sperm samples were diluted with an ionic extender containing glycerol (Gly), methanol (MeOH) and dimethyl sulfoxide (DMSO) at ratios of 5, 10 and 15 % respectively...
January 2019: Cryo Letters
H Inui, J Mizuno, E Kikuchi, K Noguchi, Y Tanji, M Hamabata, C Kotsuzumi, M Komiyama, Y Noguchi, M Tamura
BACKGROUND: Vitrification is widely used for assisted reproductive technology (ART). Most vitrification devices require the skillful placement of embryos into the carrier and aspiration of excessive vitrification solution. OBJECTIVE: To evaluate the efficacy and safety of the Cryoroom as a vitrification device. MATERIALS AND METHODS: Mouse and human embryos were vitrified with Cryoroom or Cryotop, and the developmental potency was assessed in vitro...
January 2019: Cryo Letters
F Sales, J Ferreira-Silva, J Vieira, S Basto, P Filho, J Rocha, M Moura, M Oliveira
BACKGROUND: Post-diluents could potentially increase semen cryotolerance, but remain poorly explored in horses. OBJECTIVE: The aim was to evaluate the efficiency of post-diluents on frozen-thawed semen viability of two stallions (S1-S2). MATERIALS AND METHODS: The cryopreserved semen was thawed at 50°C for 40 seconds. Semen motility and acrosin activity (AA) were determined during the thermo-resistance test (TRT). RESULTS: Progressive motility of S2 semen decreased after 60 and 90 minutes of TRT (TRT60 and TRT90) on the control compared to both post-diluents...
November 2018: Cryo Letters
A Ponomarev, V V Rodin, L Gurevich, V Melekhin, O Makeev
BACKGROUND: Xenon is an inert gas promising for the preservation of biomaterials, which forms clathrate hydrates above 0°C. OBJECTIVE: This study addresses the effect of hyperbaric xenon (P = 303 kPa) and water-xenon clathrates (P ≥ 608 kPa) on 30 days preservation of red blood cells (RBCs) at +4°C. MATERIALS AND METHODS: RBCs from healthy human donors were preserved under four different conditions: without preservatives (negative control), in CPDA-1, hyperbaric xenon, and xenon clathrate hydrates...
November 2018: Cryo Letters
H P Yadav, A Kumar, N Shah, D S Chauhan, S A Lone, D K Swain, A Saxena
BACKGROUND: The membrane and acrosomal integrity of sperm play a vital role in fertilization process; however they are compromised upon cryopreservation. OBJECTIVE: To study the effect of cholesterol-loaded cyclodextrin (CLC) on membrane and acrosome status of Hariana bull sperm during cryopreservation. MATERIALS AND METHODS: Semen samples collected from Hariana bulls with mass motility ≥ 3+ and individual progressive motility ≥ 70% were utilized in the study...
November 2018: Cryo Letters
O I Gordiyenko, S Ye Kovalenko, I F Kovalenko, V V Ogurtsova, A F Todrin
BACKGROUND: According to the two-factor theory cryodamage arises from intracellular crystallization and solution effects due to freeze concentration. OBJECTIVE: The study aims to evaluate the contribution of two types of cryodamages that are related to extra- and intracellular crystallization. METHODS: The probability of intracellular crystal formation during cooling of cell suspension in cryoprotective solution has been determined based on general thermodynamics theory...
November 2018: Cryo Letters
F Alves Pereira, C Dahl Corcini, A Cardoso da Silva, D Martins Pires, J Ribeiro Pereira, S Mari Meneghello Gheller, D Pedro Streit, A Nizio Maria, A Sergio Varela
BACKGROUND: The aim of this study was to analyze the effect of reduced glutathione (GR) and adenosine triphosphate (ATP) in cryopreserved sperm tambaqui, Colossoma macropomum.. OBJECTIVE: Semen samples were frozen at different concentrations of GR (2, 4, and 6 mM) and ATP (7.5, 15, 30 mM) evaluated separately. MATERIALS AND METHODS: Different concentrations of GR and ATP were added to the diluent Beltsville Thawing Solution 10 % of DMSO (extender control)...
November 2018: Cryo Letters
M Arguedas, A Villalobos, D Gómez, L Hernández, B E Zevallos, I Cejas, L Yabor, M Edel Martínez-Montero, J Carlos Lorenzo
BACKGROUND: Cryo-preservation of plant materials in liquid nitrogen (LN) has been described as a suitable technology to conserve genetic resources of several species. However, the potential effects of LN in the subsequent plant growth in the field should be studied before large-scale implementation of cryopreserved germplasm banks. OBJECTIVE: To describe the field performance of cryopreserved seed-derived maize adult plants. MATERIALS AND METHODS: Germination percentage and numbers of leaves and ears per plant, internodes in stems, middle - aged leaf length, plant height, ear traits and weight of 100 seeds were recorded...
November 2018: Cryo Letters
A Svoradová, L Kuželová, J Vašíček, L Olexíková, A Baláži, B Kulíková, C Hrnčár, A Ostro, M Bednarczyk, P Chrenek
BACKGROUND: Preservation of genetic resources in gene bank is necessary for conservation of endangered poultry species. OBJECTIVE: This study is to characterize Oravka rooster semen quality. MATERIALS AND METHODS: Heterospermic pool was diluted (1:1 by volume) in a freeze medium composed of a commercial diluent (Kobidil+ , K), 8% dimethyl sulphoxide (DMSO) or 8% ethylene glycol (EG) or 8% glycerol (GL), and then frozen in liquid nitrogen vapour...
November 2018: Cryo Letters
N Akyol, Ö Varışlı, S H Kızıl
BACKGROUND: The effect of the long-term storage in liquid nitrogen on semen quality has not be reported. OBJECTİVE: The study measured the spermatological parameters of bull sperm after the long-term and short-term storage. MATERIALS AND METHODS: Vintage semen (obtained from 5 Brown Swiss bulls and frozen 30 years ago) and newly frozen semen collected from 5 bulls of the same breed and prepared at the International Center for Livestock Research and Training were used...
November 2018: Cryo Letters
A Senula, D Büchner, E R J Keller, M Nagel
BACKGROUND: Vitrification approaches are widely used to cryopreserve Mentha spp. genetic resources. OBJECTIVE: Here, we compared the response of 20 different Mentha species and hybrids during cryopreservation and elucidated the efficacy of two cryoprotectants. MATERIALS AND METHODS: One hundred and fifty three Mentha spp. accessions were cryopreserved using in vitro plants maintained under slow-growth storage and PVS2 or PVS3 as cryoprotectants...
November 2018: Cryo Letters
S K Baishya, R K Biswas, K Govindasamy, B C Deka, S Sinha, M Singh
BACKGROUND: Pre-freezing treatment of boar sperm with additives improves the quality of post-thaw sperms. OBJECTIVES: The study aimed to determine the efficacy of butylated hydroxy-toluene (BHT) and cholesterol-loaded methyl-β-cyclodextrin (CLC) for the improvement of the frozen-thawed boar sperm quality. METHODS: Split samples of 30 ejaculates from six boars were cryopreserved in lactose-egg yolk-glycerol extender containing BHT (0.2 mM), CLC (5 mg/ 200-240 x 106 sperm) or BHT (0...
September 2018: Cryo Letters
K Zhu, S Mc Hossain, Y Li, F Yuan, P Hu, F Yuan
BACKGROUND: The box-in-box (BIB) has recently been developed as a reliable low-cost device with a passively controlled cooling rate. However, the typical box-in-box design is only applicable to -80°C freezer. The transfer process to liquid nitrogen may affect the quality of the sample. OBJECTIVE: To develop a smart cooling BIB that can be immersed directly into liquid nitrogen. MATERIALS AND METHODS: Different thickness and materials of the box were used to alter the cooling rate...
September 2018: Cryo Letters
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