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Journal of Nematology

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https://read.qxmd.com/read/30451440/first-report-of-carrot-cyst-nematode-heterodera-carotae-in-mexico-morphological-molecular-characterization-and-host-range-study
#1
Ilia Mariana Escobar-Avila, Edgar Óliver López-Villegas, Sergei A Subbotin, Alejandro Tovar-Soto
During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota . The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451439/-nothotylenchus-andrassy-n-sp-nematoda-anguinidae-from-northern-iran
#2
Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari
Nothotylenchus andrassy n. sp. is described and illustrated from moss ( Sphagnum sp.) based on morphology and molecular analyses. Morphologically, this new species is characterized by a medium body size, six incisures in the lateral fields, and a delicate stylet (8-9 µm long) with clearly defined knobs. Pharynx with fusiform, valveless, non-muscular and sometimes indistinct median bulb. Basal pharyngeal bulb elongated and offset from the intestine; a long post-vulval uterine sac (55% of vulva to anus distance); and elongate, conical tail with pointed tip...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451438/description-of-longidorus-azarbaijanensis-n-sp-dorylaimida-longidoridae-from-iran
#3
Farshad Gharibzadeh, Ebrahim Pourjam, Majid Pedram
Longidorus azarbaijanensis n. sp. is described and illustrated using morphological and molecular data. It was recovered in West Azarbaijan province, northwestern Iran, from the rhizospheric soil of foxtail weed. The new species is characterized by having 5.4 to 6.8 mm long females, offset, anteriorly flat lip region and separated from the rest of the body by a shallow constriction, funnel-shaped amphidial pouches, the guiding ring at 23 to 27 µm from the anterior end, 73 to 81 and 44 to 50 µm long odontostyle and odontophore, respectively, 95...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451436/molecular-characterization-and-phylogeny-of-ditylenchus-weischeri-from-cirsium-arvense-in-the-prairie-provinces-of-canada
#4
Mehrdad Madani, Mario Tenuta
Ditylenchus weischeri that parasitizes the weed Cirsium arvense (L.) Scop., 1772, (creeping thistle) was described in 2011 from Russia based on their morphology, ITS-RFLP analysis, and Hsp 90 gene sequence of a few individuals and one field collection of the plant. More recently, we found C. arvense parasitized by D. weischeri in the Prairie Provinces of Canada. Plant host preference for D. weischeri was also distinct from D. dipsaci (Kühn) Filipjev, 1936. In the current study, a comprehensive molecular analysis of many D...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451435/activity-of-vetiver-extracts-and-essential-oil-against-meloidogyne-incognita
#5
Kansiree Jindapunnapat, Nathan D Reetz, Margaret H MacDonald, Ganga Bhagavathy, Buncha Chinnasri, Noppamas Soonthornchareonnon, Anongnuch Sasnarukkit, Kamlesh R Chauhan, David J Chitwood, Susan L F Meyer
Vetiver, a nonhost grass for certain nematodes, was studied for the production of compounds active against the southern root-knot nematode, Meloidogyne incognita . In laboratory assays studying the effects on second-stage juvenile (J2) activity and viability, crude vetiver root and shoot extracts were nematotoxic, resulting in 40% to 70% J2 mortality, and were also repellent to J2. Vetiver oil did not exhibit activity against J2 in these assays. Gas chromatography-mass spectrometry analyses of three crude vetiver root ethanol extracts and a commercial vetiver oil determined that two of the major components in each sample were the sesquiterpene acid 3,3,8,8-tetramethyltricyclo[5...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451434/-stauratostoma-shelleyi-n-gen-n-sp-nematoda-rhabditida-thelastomatidae-from-appalachian-polydesmid-millipedes-polydesmida-xystodesmidae
#6
Gary Phillips, Robert J Pivar, Xiocaun Sun, John K Moulton, Ernest C Bernard
Stauratostoma shelleyi n. gen., n. sp. is described from the midgut and hindgut of nine species of the millipede family Xystodesmidae collected in the southern Appalachian regions of North Carolina, Tennessee and Alabama. Specimens of S. shelleyi were morphologically examined with differential interference contrast, phase contrast, and scanning electron microscopy. The head of S. shelleyi differs from other thelastomatid nematodes in having a head region mushroom-shaped in profile; cruciform stomatal opening formed from four flaps; greatly expanded labial disc; and eight-sectored annule-like column supporting the labial disc...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451433/morphological-re-description-and-18-s-rdna-sequence-confirmation-of-the-pinworm-aspiculuris-tetraptera-nematoda-heteroxynematidae-infecting-the-laboratory-mice-mus-musculus
#7
Rewaida Abdel-Gaber, Fathy Abdel-Ghaffar, Saleh Al Quraishy, Kareem Morsy, Rehab Saleh, Heinz Mehlhorn
Aspiculuris tetraptera is a heteroxynematid nematoda infecting most of the laboratory animals, occasionally mice which represent the mostly used animal for biological, medical, and pharmacological studies. The present study aimed to investigate the prevalence of nematode parasites infection in the laboratory mice Mus musculus in Egypt. Morphologically, this oxyurid possessed four distinct cephalic papillae on a cephalic plate, with three small rudimental lips carrying two sessile poorly developed labial papillae and one pair of amphidial pores...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451432/nematode-genome-announcement-a-draft-genome-for-rice-root-knot-nematode-meloidogyne-graminicola
#8
Vishal Singh Somvanshi, Madhura Tathode, Rohit Nandan Shukla, Uma Rao
The rice root-knot nematode Meloidogyne graminicola has emerged as a devastating pest of rice in South-East Asian countries. Here we present a draft genome sequence for M. graminicola , assembled using data from short and long insert libraries sequenced on Illumina GAIIx sequencing platform.
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451431/molecular-identification-of-entomopathogenic-nematode-isolates-from-the-philippines-and-their-biological-control-potential-against-lepidopteran-pests-of-corn
#9
Barbara L Caoili, Romnick A Latina, Regina Faye C Sandoval, Joey I Orajay
In search for local entomopathogenic nematode (EPN) species as a biological control agent of lepidopterous insect pests of corn, a survey for EPN in the major islands in the Philippines was conducted. Seven EPN populations from 279 soil samples were isolated using Ostrinia furnacalis, the key target insect pest of corn in the country, as bait. Analysis of the ITS1-5.8S-ITS2 ribosomal DNA sequence revealed the presence of Steinernema abbasi, Steinernema minutum , Steinernema tami , and Heterorhabditis indica ...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451430/revisiting-the-life-cycle-of-pasteuria-penetrans-infecting-meloidogyne-incognita-under-soil-less-medium-and-effect-of-streptomycin-sulfate-on-its-development
#10
Victor Phani, Uma Rao
Pasteuria penetrans is a Gram-positive, endospore forming soil bacterium, infecting root-knot nematodes, Meloidogyne spp. Being obligate in nature, the bacterium is not easily grown in vitro, and the in vivo culturing technique is relied on the soil-based microcosm since long. Hence, culturing of P. penetrans using CYG germination pouches as a soil-less medium for plant growth, promises to provide a contamination free environment along with ease in isolation of infected females from the plant roots. Additionally, this method increases the percentage of P...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30451429/an-agent-based-metapopulation-model-simulating-virus-based-biocontrol-of-heterodera-glycines
#11
Safyre Anderson, Chinmay Soman, Sadia Bekal, Leslie Domier, Kris Lambert, Kaustubh Bhalerao
With recently discovered soybean cyst nematode (SCN) viruses, biological control of the nematodes is a theoretical possibility. This study explores the question of what kinds of viruses would make useful biocontrol agents, taking into account evolutionary and population dynamics. An agent-based model, Soybean Cyst Nematode Simulation (SCNSim), was developed to simulate within-host virulence evolution in a virus-nematode-soybean ecosystem. SCNSim was used to predict nematode suppression under a range of viral mutation rates, initial virulences, and release strategies...
September 3, 2018: Journal of Nematology
https://read.qxmd.com/read/30335914/first-report-of-the-gastropod-killing-nematode-phasmarhabditis-hermaphrodita-in-oregon-u-s-a
#12
Rory J Mc Donnell, Marisa S Lutz, Dana K Howe, Dee R Denver
No abstract text is available yet for this article.
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335913/delatylus-andersoni-n-gen-n-sp-nematoda-neotylenchidae-isolated-from-white-pine-pinus-monticola-lumber-from-usa-and-intercepted-in-ningbo-china
#13
Qing Yu, Maria Munawar, Jianfeng Gu, Weimin Ye
Three populations of neotylenchid nematodes were isolated in Ningbo, P. R. China, from white pine lumber (Pinus monticola) imported from the USA. The nematodes were morphologically intermediate between Hexatylus and Deladenus. The nematodes were molecularly characterized based on sequences of the rDNA small subunit 18S, large subunit 28S D2/D3, and internal transcribed spacer sequences. The phylogenetic inferences placed the nematodes with other neotylenchid nematodes, i.e., Fergusobia and Rubzovinema. Based on the morphology and phylogenetic analysis, this nematode is described herein as Delatylus andersoni n...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335912/list-of-type-specimens-deposited-since-1998-in-the-united-states-department-of-agriculture-nematode-collection-beltsville-maryland
#14
Zafar A Handoo, Mihail R Kantor, Lynn K Carta, David J Chitwood
The United States Department of Agriculture Nematode Collection (USDANC) is one of the largest and most valuable in existence and includes millions of specimens housed in over 39,800 permanent slides and 9,300 vials. This collection preserves type specimens of nematodes to serve as a reference for identifications and future taxonomic revisions. Also, the collection provides useful information on nematode hosts, occurrence, and distribution. The present list includes type specimens added to the USDANC since 1998...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335911/identification-of-hg-types-of-soybean-cyst-nematode-heterodera-glycines-and-resistance-screening-on-soybean-genotypes-in-northeast-china
#15
Cui Hua, Chunjie Li, Yanfeng Hu, Yanzhi Mao, Jia You, Mingze Wang, Jingsheng Chen, Zhongyan Tian, Congli Wang
Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is a serious soybean pathogen worldwide. HG Type 0 had been a predominant SCN in Heilongjiang province, the largest soybean (Glycine max L.) producing region in China. Recently, increased virulence on resistant cultivars originally developed for resistance to HG Type 0 was observed in fields. In order to identify new cultivars resistant to local SCN populations, two soil samples were collected from two counties (Anda and Wuchang) in which increased virulence on resistant cultivars occurred, and single-cyst cultures from each soil sample were maintained for more than five generations...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335910/influence-of-temperature-on-susceptibility-of-cvs-tifguard-and-georgia-06g-peanut-to-meloidogyne-arenaria
#16
Weimin Yuan, C C Holbrook, Y Chu, P Ozias-Akins, D W Dickson
Tifguard was released in 2008 as a peanut cultivar with a high level of resistance to Meloidogyne arenaria. Our objective was to determine the role of temperature on infection and development of M. arenaria in Tifguard compared to that in the nematode susceptible cultivar, Georgia-06G. Temperature affected the rate of nematode infection and development in both Tifguard and Georgia-06G (P ≤ 0.05). In Georgia-06G, egg-laying females were observed 25, 20 or 25 days after inoculation at 28°C, 31°C, and 34°C, respectively...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335909/postembryonic-ventral-nerve-cord-development-and-gonad-migration-in-steinernema-carpocapsae
#17
Hung Xuan Bui, Nathan E Schroeder
Steinernema carpocapsae is an entomopathogenic nematode widely studied for its properties as a biocontrol agent in insect pest management and as a model for understanding bacterial symbioses. Less attention has been given to the development of specific anatomical structures within S. carpocapsae. A better understanding of entomopathogenic nematode development and anatomy may lead to improved biocontrol efficacy. We recently demonstrated that the neuroanatomy of S. carpocapsae IJs differs from the dauer stage of Caenorhabditis elegans...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335908/incidence-of-oscheius-onirici-nematoda-rhabditidae-a-potentially-entomopathogenic-nematode-from-the-marshlands-of-wisconsin-usa
#18
Weimin Ye, Shane Foye, Ann E MacGuidwin, Shawn Steffan
In a search for an entomopathogenic nematode to control cranberry insect pests, three Oscheius populations (Rhabditidae) were recovered through the Galleria-bait method from one sample taken in a wild cranberry marsh in Jackson County, Wisconsin, USA. Morphological studies with light microscopy and scanning electron microscopy, as well as molecular analyses of the near-full-length small subunit rDNA gene, D2/D3 expansion segments of the large subunit rDNA gene, internal transcribed spacer, and mitochondrial cytochrome oxidase subunit 1 (CoxI) genes revealed this as Oscheius onirici, a species recently described from a karst cave soil of central Italy...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30335907/chemotaxis-of-caenorhabditis-elegans-toward-volatile-organic-compounds-from-stropharia-rugosoannulata-induced-by-amino-acids
#19
Jing Bian, Haili Zhang, Shuai Meng, Yajun Liu
A variety of natural substances including both volatile organic compounds and water-soluble compounds play a significant role in the chemotactic behaviors of the model nematode Caenorhabditis elegans. We observed chemotactic behaviors of C. elegans with respect to response to attractants produced by nematode parasitic fungus Stropharia rugosoannulata, which were partially induced by specific amino acids. The results of gas chromatography-mass spectrometer analysis suggested that 1-octen-3-ol was produced and benzaldehyde concentrations increased when L-phenylalanine was added to water agar plate...
May 31, 2018: Journal of Nematology
https://read.qxmd.com/read/30451428/first-report-of-the-yellow-nutsedge-cyst-nematode-heterodera-cyperi-in-georgia-u-s-a
#20
Abolfazl Hajihassani, Bhabesh Dutta, Ganpati B Jagdale, Sergei A Subbotin
Soil samples collected during a survey for plant-parasitic nematodes in Tift County GA in summer 2017 were submitted for routine diagnosis of nematodes to the Extension Nematology Lab at the Department of Plant Pathology, University of Georgia, Athens, Georgia. Cyst nematodes recovered by centrifugal flotation technique were discovered in the samples from two research sites in a field with a history of tobacco and vegetable production. Cyst nematodes from tobacco (10 cysts/100 cm 3 of soil) and vegetable (2 cysts/100 cm 3 of soil) sites had similar morphological features...
2018: Journal of Nematology
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