Rui Zhao, Wen-Ai Wu, Yi-Hua Huang, Xin-Kai Li, Jia-Qi Han, Wu Jiao, Yin-Na Su, He Zhao, Yang Zhou, Wu-Qiang Cao, Xun Zhang, Wei Wei, Wan-Ke Zhang, Qing-Xin Song, Xin-Jian He, Biao Ma, Shou-Yi Chen, Jian-Jun Tao, Cui-Cui Yin, Jin-Song Zhang
Gene expression is regulated at multiple levels, including RNA processing and DNA methylation/demethylation. How these regulations are controlled remains unclear. Here, through analysis of a suppressor for the OsEIN2 over-expressor, we identified an RNA recognition motif protein SUPPRESSOR OF EIN2 (SOE). SOE is localized in nuclear speckles and interacts with several components of the spliceosome. We find SOE associates with hundreds of targets and directly binds to a DNA glycosylase gene DNG701 pre-mRNA for efficient splicing and stabilization, allowing for subsequent DNG701-mediated DNA demethylation of the transgene promoter for proper gene expression...
March 20, 2024: New Phytologist