journal
https://read.qxmd.com/read/38185743/mixed-eimeria-and-cryptosporidium-infection-and-its-effects-on-pathology-and-clinical-outcomes-in-juvenile-asian-seabass-lates-calcarifer-cultured-in-thailand
#61
JOURNAL ARTICLE
Watcharapol Suyapoh, Narissara Keawchana, Peerapon Sornying, Sirikachorn Tangkawattana, Pokphon Khirilak, Sasibha Jantrakajorn
Coccidiosis is an important disease in juvenile fish because of severe intestinal injury during infection. We first reported the mixed infection of intestinal coccidia and its association with health status and pathological findings in juvenile Asian seabass (Lates calcarifer) cultured in Thailand. Two groups of Asian seabass, 60-day fish and 90-day fish, were sampled to investigate prevalence and coccidian infection intensity using morphological characterization and PCR. Phylogenetic analysis of 18S rRNA gene amplified from the intestines revealed Eimeria sp...
January 7, 2024: Journal of Fish Diseases
https://read.qxmd.com/read/38153008/influence-of-probiotic-and-prebiotic-supplementation-on-intestinal-microbiota-and-resistance-to-edwardsiella-ictaluri-infection-in-channel-catfish-ictalurus-punctatus-following-florfenicol-administration
#62
JOURNAL ARTICLE
Caitlin E Older, Matt J Griffin, Bradley M Richardson, Geoffrey C Waldbieser, J Grant Reifers, Penelope M Goodman, Cynthia Ware, Delbert M Gatlin, David J Wise, Fernando Y Yamamoto
Enteric septicemia of catfish (ESC), caused by the gram-negative enteric bacteria Edwardsiella ictaluri, is a significant threat to catfish aquaculture in the southeastern United States. Antibiotic intervention can reduce mortality; however, antibiotic use results in an imbalance, or dysbiosis, of the gut microbiota, which may increase susceptibility of otherwise healthy fish to enteric infections. Herein, recovery of the intestinal microbiota and survivability of channel catfish in response to ESC challenge was evaluated following a 10-day course of florfenicol and subsequent probiotic or prebiotic supplementation...
December 28, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38151724/study-of-the-frequencies-of-erythrocyte-abnormalities-as-in%C3%A2-situ-biomarkers-of-genotoxic-risk-of-chemicals-in-special-fish-stock-in-water-supply-reservoirs
#63
JOURNAL ARTICLE
Jiří Řehulka, Jaroslav Bradík
In three water-supply reservoirs in the catchment area of the Odra River (Czech Republic), a special fish stock was monitored for control of health to estimate the mutagenic effect of chemicals. The results contribute to obtaining initial information about the morphology of erythrocyte abnormalities classified in 21 categories in 17 fish species in reservoirs with abundant salmonids (the Morávka Reservoir) or with the prevalence of cyprinids (the Kružberk and Šance Reservoirs), not directly exposed to the adverse environmental effects such as industrial, urban, agricultural and intensive farming activities...
December 27, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38146082/basibranchial-structure-affecting-cardiac-morphology-in-atlantic-salmon-salmo-salar-l
#64
JOURNAL ARTICLE
Trygve T Poppe, Anne Katrine Reed, Helene Wisløff
No abstract text is available yet for this article.
December 25, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38146073/molecular-quantification-of-parasitic-sea-louse-larvae-depends-on-species-and-life-stage
#65
JOURNAL ARTICLE
Nathan E Mertz, Rachel A Paterson, Bengt Finstad, Hege Brandsegg, Ida Pernille Øystese Andersskog, Frode Fossøy
Sea lice cause substantial economic and environmental harm to Norway's aquaculture industry and wild salmonid populations. Rapid, accurate quantification of lice larval densities in coastal waters remains the greatest bottleneck for providing empirical data on infestation risk within wild salmon habitats and aquaculture production regions. We evaluated the capability of droplet digital PCR (ddPCR) as an absolute quantification method for the planktonic stages of two parasitic louse species, Lepeophtheirus salmonis (Krøyer) and Caligus elongatus (von Nordman)...
December 25, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38115621/effect-of-temperature-on-the-protective-efficacy-of-a-live-attenuated-vaccine-against-herpesviral-haematopoietic-necrosis-in-goldfish
#66
JOURNAL ARTICLE
Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
The live attenuated vaccine P7-P8 strain against herpesviral haematopoietic necrosis, which is caused by cyprinid herpesvirus 2 (CyHV-2), exhibits high protective efficacy in goldfish at 25°C, the predominant temperature for this disease; however, the effect of water temperature during the vaccination period on efficacy has not been determined. In this study, an in vitro experiment revealed that the vaccine strain grew between 15 and 30°C in the goldfish cell line RyuF-2. Subsequent in vivo efficacy tests were conducted with vaccination temperatures ranging from 15 to 30°C...
December 19, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38112174/development-and-application-of-a-recombinase-aided-amplification-combined-with-a-lateral-flow-dipstick-assay-for-rapid-visual-detection-of-anguillid-herpesvirus-1
#67
JOURNAL ARTICLE
Xi Chen, Hua Chen, Jun-Qing Ge
Eel (Anguilla sp.) is an important freshwater-cultured species with high economic value in China. Anguillid herpesvirus 1 (AngHV-1) has been proven to be the pathogen of "mucus sloughing and haemorrhagic septicaemia disease" in eels, resulting in significant mortality and substantial losses to the eel industry. Current diagnostic methods for detecting AngHV-1 are limited to laboratory-based tests, for example, conventional end-point PCR and qPCR. Therefore, there is an urgent need to develop an accurate, rapid, and simple detection method for on-site diagnosis of AngHV-1...
December 19, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38087880/diversity-of-fungus-like-stramenopilous-organisms-oomycota-in-lithuanian-freshwater-aquaculture-morphological-and-molecular-analysis-risk-to-fish-health
#68
JOURNAL ARTICLE
Svetlana Markovskaja, Reda Iršėnaitė, Audrius Kačergius, Gintarė Sauliutė, Milda Stankevičiūtė
The present work is the first comprehensive study of fungus-like stramenopilous organisms (Oomycota) diversity in Lithuanian fish farms aimed at proper identification of saprolegniasis pathogens, which is important for water quality control, monitoring infection levels and choosing more effective treatments for this disease in aquaculture. Pathogenic to fish, Saprolegnia and other potentially pathogenic water moulds were isolated from adult fish, their eggs, fry and from water samples. All detected isolates were examined morphologically and confirmed by sequence-based molecular methods...
December 12, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38073005/confirmatory-test-of-active-ihhnv-infection-in-shrimp-by-immunohistochemistry-and-ihhnv-longamp-pcr
#69
JOURNAL ARTICLE
Supapong Imsonpang, Arnon Pudgerd, Charoonroj Chotwiwatthanakun, Jiraporn Srisala, Piyachat Sanguanrut, Chanadda Kasamechotchung, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Rapeepun Vanichviriyakit
The presence of endogenous viral elements (EVE) in the penaeid shrimp genome has been recently reported and suggested to be involved in the host recognition of viral invaders. Our previous report of a search for EVE of infectious hypodermal and haematopoietic necrosis virus (IHHNV-EVE) in the Thai Penaeus monodon whole genome sequence project (GenBank accession no. JABERT000000000) confirmed the presence of three clusters of EVE derived from IHHNV in the shrimp genome. This study aimed to compare an immunohistochemistry method (IHC) and a PCR method to detect infectious IHHNV infection in shrimp...
December 10, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38069492/identification-of-a-potential-antigen-stimulating-immune-response-against-vibrio-parahaemolyticus-infection-in-hybrid-tilapia-oreochromis-aureus%C3%A2-%C3%A2-%C3%A3-%C3%A2-oreochromis-niloticus%C3%A2
#70
JOURNAL ARTICLE
Qingsong Zeng, Yunxiao Sun, Peifang Lai, Mingqin Huang, Ximing Peng, Junjie Huang, Qintao Chen, Yanxu Chen, Huaqian Wang
Vibrio parahaemolyticus (V. parahaemolyticus) is a major pathogen that causes substantial losses in the marine fishery. With the emergence of antibiotic resistance, vaccines have become the most effective approach against V. parahaemolyticus infection. Adhesion factors on the cell surface are pivotal in the colonization and pathogenesis of V. parahaemolyticus within the host, highlighting their potential as vaccine candidates. This study aims to assess the immunogenicity and potential of recombinant V. parahaemolyticus MAM7 (rMAM7) as a vaccine candidate...
December 8, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38062566/establishment-of-a-taqman-probe-based-qpcr-assay-for-detecting-microsporidia-enterospora-epinepheli-in-grouper
#71
JOURNAL ARTICLE
Quan-Lin Liu, Yuan Wang, Jie Chen, Guo-Qing Pan, Yan-Feng Yue, Ze-Yang Zhou, Wen-Hong Fang
Enterospora epinepheli is an intranuclear microsporidian parasite causing serious emaciative disease in hatchery-bred juvenile groupers (Epinephelus spp.). Rapid and sensitive detection is urgently needed as its chronic infection tends to cause emaciation as well as white faeces syndrome and results in fry mortality. This study established a TaqMan probe-based real-time quantitative PCR assays targeting the small subunit rRNA (SSU) gene of E. epinepheli. The relationship between the standard curve of cycle threshold (Ct) and the logarithmic starting quantity (SQ) was determined as Ct = -3...
December 7, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38058214/magnetic-resonance-imaging-shows-spinal-curvature-in-chinook-salmon-oncorhynchus-tshawytscha-is-associated-with-chronic-inflammation-of-peri-vertebral-soft-tissues
#72
JOURNAL ARTICLE
Bailey A Lovett, Elwyn C Firth, Matthew R Perrott, John S Munday, Beau P Pontre, Anna-Maria P Lydon, Jane E Symonds, Mark A Preece, Neill A Herbert
Chinook salmon (Oncorhynchus tshawytscha) farmed in New Zealand are known to develop abnormal spinal curvature late in seawater production. Its cause is presently unknown, but there is evidence to suggest a neuromuscular pathology. Using magnetic resonance imaging (MRI), we evaluated the relationship between soft tissue pathology and spinal curvature in farmed Chinook salmon. Regions of interest (ROIs) presenting as pathologic MRI signal hyper-intensity were identified from scans of 24 harvest-sized individuals: 13 with radiographically-detectable spinal curvature and 11 without...
December 6, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38054569/disruption-of-a-kasb-homolog-gene-kasb-causes-attenuation-of-cell-invasion-and-virulence-of-nocardia-seriolae
#73
JOURNAL ARTICLE
Su-Ming Zhou, Feng-Ling Shu, Hao Liu, Dong Qian, Jia-Song Xie, Ya-Jun Wang, Fei Yin
Nocardia seriolae is the primary aetiological agent of nocardiosis in fish, which causes mass mortality in freshwater and marine fish. β-ketoacyl-ACP synthase (KAS) is one of the essential enzymes in the synthesis of mycolic acids (MASs) in Mycobacterium spp. and has been chosen as the target for therapeutic intervention in mycobacterial diseases. In the present study, a kasB homologue gene (kasB) was identified in the genome of N. seriolae, and the gene-deficient mutant (ΔkasB) was generated based on a clinical isolate, XSYC-Ns...
December 6, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38041393/the-role-of-dietary-vitamin-a-in-mechanisms-of-cataract-development-in-the-teleost-lumpfish-cyclopterus-lumpus-l
#74
JOURNAL ARTICLE
Margret J Deering, Hélène Paradis, Raahyma Ahmad, Adil S Al-Mehiawi, Robert L Gendron
Lumpfish (Cyclopterus lumpus L) are highly prone to cataract development in the wild and in culture. There is evidence that cataract in farmed fish is related to nutrition. However, both the nutrients and the mechanisms involved in cataract development in lumpfish are not clear. Here we investigated the mechanisms involved and the role of dietary vitamin A in cataract development in a cultured lumpfish population. Cultured lumpfish were fed three diets differing only in vitamin A supplementation level (5000, 15,000 and 120,000 IU/kg) over an 18-month period, and fish weight, cataract frequencies and severities were determined...
December 1, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38041240/iron-supplementation-in-the-diets-of-hybrid-catfish-ictalurus-punctatus%C3%A2-%C3%A3-%C3%A2-i-%C3%A2-furcatus-juveniles-affected-haematocrit-levels-and-potentially-decreased-disease-resistance-to-edwardsiella-ictaluri
#75
JOURNAL ARTICLE
Fernando Y Yamamoto, Matt J Griffin, Bradley M Richardson, Justin M Stilwell, Nicholas Romano, Penelope M Goodman, J Grant Reifers, David J Wise
To prevent catfish idiopathic anaemia, diets fortified with iron have been adopted as a regular practice on commercial catfish farms to promote erythropoiesis. However, the effects of prolonged exposure of excess dietary iron on production performance and disease resistance for hybrid catfish (Ictalurus punctatus × I. furcatus) remains unknown. Four experimental diets were supplemented with ferrous monosulphate to provide 0, 500, 1000, and 1500 mg of iron per kg of diet. Groups of 16 hybrid catfish juveniles (~22...
December 1, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38038138/gonadal-neoplasia-in-chinese-gold-barbs-barbodes-semifasciolatus-from-a-home-aquarium
#76
JOURNAL ARTICLE
Justin M Stilwell, Alvin C Camus, Natalie K Stilwell
No abstract text is available yet for this article.
December 1, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38031507/a-case-study-of-epizootic-ulcerative-syndrome-eus-caused-by-aphanomyces-invadans-in-eastern-mosquitofish-gambusia%C3%A2-holbrooki-from-the-headwaters-of-charleston-harbor-south-carolina
#77
JOURNAL ARTICLE
Timothy J Bruce, Carl C Trettin, Zachary A Noel, Alex T Chow, Kylie Warden, Craig Roghair, Troy M Farmer
No abstract text is available yet for this article.
November 30, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38031399/phenotypic-and-genomic-characterization-of-a-non-pathogenic-epilithonimonas-ginsengisoli-isolated-from-diseased-farmed-rainbow-trout-oncorhynchus-mykiss-in-chile
#78
JOURNAL ARTICLE
Claudio D Miranda, Rute Irgang, Christopher Concha, Rodrigo Rojas, Ruben Avendaño-Herrera
Flavobacterial infection associated with diseased fish is caused by multiple bacterial species within the family Flavobacteriaceae. In the present study, the Chilean isolate FP99, from the gills of a diseased, farmed rainbow trout (Oncorhynchus mykiss), was characterized using phenotypic and genomic analyses. Additionally assessed was pathogenic activity. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that isolate FP99 belonged to the genus Epilithonimonas, an average nucleotide identity value of 100% was detected with the Chilean isolate identified as Epilithonimonas sp...
November 29, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38018296/influence-of-colour-background-on-anti-viral-activity-against-viral-haemorrhagic-septicaemia-virus-in-zebrafish-regulated-by-circadian-rhythm-signalling-pathway
#79
JOURNAL ARTICLE
Se-Hyun Cho, Se-Young Cho, Bipin Vaidya, Joseph Kwon, Duwoon Kim
The influence of colour background on the regulation of behavioural and physiological responses in zebrafish is widely recognized. However, its specific effect on virus infection in zebrafish remains unclear. This study aimed to explore the susceptibility of zebrafish to viral haemorrhagic septicaemia virus (VHSV) infection in relation to background colour, investigate the underlying mechanisms, and elucidate the involvement of key molecules, using proteomic and gene expression analyses. The results revealed that zebrafish housed in a blue tank exhibited higher survival rates and considerably reduced VHSV replication compared to those housed in a yellow tank...
November 28, 2023: Journal of Fish Diseases
https://read.qxmd.com/read/38014710/phenotypic-and-genomic-insights-into-the-pathogenicity-and-antimicrobial-resistance-of-an-enterobacter-roggenkampii-strain-isolated-from-diseased-silver-arowana-osteoglossum-bicirrhosum
#80
JOURNAL ARTICLE
Yuerui Li, Zhongshi Sun
Enterobacter roggenkampii is an opportunistic pathogen that causes infections in a wide range of hosts. A bacterial strain named EOBSR_19 was isolated from diseased silver arowana, Osteoglossum bicirrhosum. This bacterium was identified as E. roggenkampii based on the phenotypic characteristics and sequence analysis of the16S rDNA and gyrB genes. Average nucleotide identity and phylogenetic analysis based on the whole genome sequence further confirmed the bacterial taxonomy of EOBSR_19. Artificial experimental infection indicated that EOBSR_19 was pathogenic to fish...
November 28, 2023: Journal of Fish Diseases
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