A Nafeez Ahmed, S Mithra, S Suryakodi, G Taju, M J Abdul Wazith, K Kanimozhi, V Rajkumar, A Badhusha, S Abdul Majeed, A S Sahul Hameed
A permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub-cultured more than 75 passages and showed optimal growth at 28°C using L-15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial-like cells. The SPB cells were tested for mycoplasma contamination which was found to be negative. The origin of the SPB cell line from T...
January 29, 2024: Journal of Fish Diseases
Sonia A Soto-Rodriguez, Francis I Marrujo Lopez, Karla G Aguilar-Rendon, Rafael Hernández Guzmán
The present study investigates molecular-based PCR techniques to estimate the prevalence of fish pathogens in southwest Mexico where recurrent mortality in the tilapia cultures has been observed. Sample of internal organs and lesions of Nile tilapia were taken and analysed in 2018, 2019, 2020 and 2022 to detect bacterial pathogens using PCR. No samples were taken in 2021 due to the COVID-19 pandemic. The real-time PCR conditions were optimized to allow a qualitative reliable detection of the bacteria from fixed fish tissue...
January 25, 2024: Journal of Fish Diseases
Hee-Jae Choi, Da-Yeon Choi, Ji-Hoon Lee, Jun-Hwan Kim, Yue Jai Kang
No abstract text is available yet for this article.
January 24, 2024: Journal of Fish Diseases
Irene Maja Nanni, Perla Tedesco, David Baldo, Roberta Galuppi, Marina Collina
No abstract text is available yet for this article.
January 24, 2024: Journal of Fish Diseases
Alexandra K Mason, Lance Adams, Michael M Garner
Sixteen whitespotted bamboo sharks (Chiloscyllium plagiosum) with histologically similar bacterial abscesses were submitted to Northwest ZooPath from nine zoo and aquarium institutions over a 17-year period. These abscesses were characterized by inflammatory cell infiltrates and necrosis with intralesional small, Gram-positive, acid-fast negative, cocci bacteria. The clinical presentation, histologic findings, and culture results indicate that Enterococcus faecalis is a relatively common cause of these lesions in whitespotted bamboo sharks...
January 19, 2024: Journal of Fish Diseases
Haakon Hansen, Bjørn Spilsberg, Sigmund Sevatdal, Trine Sakariassen, Christoph Hahn, Saima Nasrin Mohammad, Egil Karlsbakk
Detection of intestinal parasites in fish typically requires autopsy, resulting in the sacrifice of the fish. Here, we describe a non-lethal method for detecting the tapeworm Eubothrium crassum in fish using anal swabs and real-time PCR detection. Two assays were developed to detect cytochrome oxidase I (COI) mitochondrial DNA and 18S ribosomal DNA sequences of E. crassum, respectively. The assays were tested on swab samples from confirmed pathogen free Atlantic salmon (Salmo salar L.) and on samples from farmed Atlantic salmon, where the presence and intensity of parasites had been established through autopsy...
January 18, 2024: Journal of Fish Diseases
John Brian Jones, Cara L Brosnahan, Anjali Pande
Lobsters and crayfish in Australasia can develop a condition known as Tail Fan Necrosis (TFN syndrome). Many attempts have been made to find a primary pathogen or link the syndrome to commercial activities, but a solution remains elusive. TFN syndrome is a 'wicked problem', a problem difficult or impossible to solve because of incomplete and contradictory information forming a matrix of potential outcomes with no simple solution. Reviewing the literature shows TFN syndrome is sometimes reported to develop in association with sterile blisters on the telson and uropods which may rupture permitting invasion by environmental fungal and/or bacterial flora...
January 16, 2024: Journal of Fish Diseases
Srirengaraj Vijayaram, Hary Razafindralambo, Yun Zhang Sun, Giuseppe Piccione, Cristiana Roberta Multisanti, Caterina Faggio
Nanotechnology is an expanding and new technology that prompts production with nanoparticle-based (1-100 nm) organic and inorganic materials. Such a tool has an imperative function in different sectors like bioengineering, pharmaceuticals, electronics, energy, nuclear energy, and fuel, and its applications are helpful for human, animal, plant, and environmental health. In exacting, the nanoparticles are synthesized by top-down and bottom-up approaches through different techniques such as chemical, physical, and biological progress...
January 16, 2024: Journal of Fish Diseases
Mengmeng Chen, Mingyang Xue, Jingtao Chen, Zidong Xiao, Xiaowei Hu, Chunjie Zhang, Nan Jiang, Yuding Fan, Yan Meng, Yong Zhou
Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle (Pelodiscus sinensis), then named strain JDP-FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature-sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP-FX...
January 13, 2024: Journal of Fish Diseases
Zhicheng Li, Jingyu Zhuang, Jiaming Chen, Jizhen Cao, Qing Han, Zhi Luo, Baotun Wang, Hebing Wang, Anxing Li
Amyloodinium ocellatum is among the most devastating protozoan parasites, causing huge economic losses in the mariculture industry. However, the pathogenesis of amyloodiniosis remains unknown, hindering the development of targeted anti-parasitic drugs. The A. ocellatum in vitro model is an indispensable tool for investigating the pathogenic mechanism of amyloodiniosis at the cellular and molecular levels. The present work developed a new cell line, ALG, from the gill of yellowfin seabream (Acanthopagrus latus)...
January 13, 2024: Journal of Fish Diseases
Allison L Wise, Benjamin R LaFrentz, Anita M Kelly, Mark R Liles, Matt J Griffin, Benjamin H Beck, Timothy J Bruce
Flavobacterium covae and virulent Aeromonas hydrophila are prevalent bacterial pathogens within the US catfish industry that can cause high mortality in production ponds. An assessment of in vivo bacterial coinfection with virulent A. hydrophila (ML09-119) and F. covae (ALG-00-530) was conducted in juvenile channel catfish (Ictalurus punctatus). Catfish were divided into seven treatments: (1) mock control; (2) and (3) high and low doses of virulent A. hydrophila; (4) and (5) high and low doses of F. covae; (6) and (7) simultaneous challenge with high and low doses of virulent A...
January 12, 2024: Journal of Fish Diseases
Huanrong He, Xinzhi Huang, Caiyi Wen, Chun Liu, Biao Jiang, Yanhua Huang, Youlu Su, Wei Li
The purpose of this study was to investigate whether a defensin-like antimicrobial peptide (C-13326 peptide) identified in Hermetia illucens could possess protective effect against multidrug-resistant Aeromonas schubertii in hybrid snakehead (Channa maculate ♀ × Channa argus ♂). The cDNA of C-13326 peptide comprised 243 nucleotides encoding 80 amino acids, with six conserved cysteine residues and the classical CSαβ structure. The recombinant expression plasmid pPIC9K-C-13326 was constructed and transformed into GS115 Pichia pastoris, and the C-13326 peptide was expressed by induction with 1% methanol...
January 10, 2024: Journal of Fish Diseases
Fei Yang, Bin Yang, Kaige Song, Yingjie Jin, Gaoxue Wang, Pengfei Li, Qing Yu, Fei Ling
Micropterus salmoides rhabdovirus (MSRV) is a formidable pathogen, presenting a grave menace to juvenile largemouth bass. This viral infection frequently leads to epidemic outbreaks, resulting in substantial economic losses within the aquaculture industry. Unfortunately, at present, there are no commercially available vaccines or pharmaceutical treatments to combat this threat. In order to address the urgent need for therapeutic strategy to resist MSRV infection, the antiviral activity of natural product honokiol against MSRV was explored in this study...
January 8, 2024: Journal of Fish Diseases
Watcharapol Suyapoh, Narissara Keawchana, Peerapon Sornying, Sirikachorn Tangkawattana, Pokphon Khirilak, Sasibha Jantrakajorn
Coccidiosis is an important disease in juvenile fish because of severe intestinal injury during infection. We first reported the mixed infection of intestinal coccidia and its association with health status and pathological findings in juvenile Asian seabass (Lates calcarifer) cultured in Thailand. Two groups of Asian seabass, 60-day fish and 90-day fish, were sampled to investigate prevalence and coccidian infection intensity using morphological characterization and PCR. Phylogenetic analysis of 18S rRNA gene amplified from the intestines revealed Eimeria sp...
January 7, 2024: Journal of Fish Diseases
Caitlin E Older, Matt J Griffin, Bradley M Richardson, Geoffrey C Waldbieser, J Grant Reifers, Penelope M Goodman, Cynthia Ware, Delbert M Gatlin, David J Wise, Fernando Y Yamamoto
Enteric septicemia of catfish (ESC), caused by the gram-negative enteric bacteria Edwardsiella ictaluri, is a significant threat to catfish aquaculture in the southeastern United States. Antibiotic intervention can reduce mortality; however, antibiotic use results in an imbalance, or dysbiosis, of the gut microbiota, which may increase susceptibility of otherwise healthy fish to enteric infections. Herein, recovery of the intestinal microbiota and survivability of channel catfish in response to ESC challenge was evaluated following a 10-day course of florfenicol and subsequent probiotic or prebiotic supplementation...
December 28, 2023: Journal of Fish Diseases
Jiří Řehulka, Jaroslav Bradík
In three water-supply reservoirs in the catchment area of the Odra River (Czech Republic), a special fish stock was monitored for control of health to estimate the mutagenic effect of chemicals. The results contribute to obtaining initial information about the morphology of erythrocyte abnormalities classified in 21 categories in 17 fish species in reservoirs with abundant salmonids (the Morávka Reservoir) or with the prevalence of cyprinids (the Kružberk and Šance Reservoirs), not directly exposed to the adverse environmental effects such as industrial, urban, agricultural and intensive farming activities...
December 27, 2023: Journal of Fish Diseases
Trygve T Poppe, Anne Katrine Reed, Helene Wisløff
No abstract text is available yet for this article.
December 25, 2023: Journal of Fish Diseases
Nathan E Mertz, Rachel A Paterson, Bengt Finstad, Hege Brandsegg, Ida Pernille Øystese Andersskog, Frode Fossøy
Sea lice cause substantial economic and environmental harm to Norway's aquaculture industry and wild salmonid populations. Rapid, accurate quantification of lice larval densities in coastal waters remains the greatest bottleneck for providing empirical data on infestation risk within wild salmon habitats and aquaculture production regions. We evaluated the capability of droplet digital PCR (ddPCR) as an absolute quantification method for the planktonic stages of two parasitic louse species, Lepeophtheirus salmonis (Krøyer) and Caligus elongatus (von Nordman)...
December 25, 2023: Journal of Fish Diseases
Hiroaki Saito, Shungo Minami, Manami Yuguchi, Aiko Shitara, Hidehiro Kondo, Goshi Kato, Motohiko Sano
The live attenuated vaccine P7-P8 strain against herpesviral haematopoietic necrosis, which is caused by cyprinid herpesvirus 2 (CyHV-2), exhibits high protective efficacy in goldfish at 25°C, the predominant temperature for this disease; however, the effect of water temperature during the vaccination period on efficacy has not been determined. In this study, an in vitro experiment revealed that the vaccine strain grew between 15 and 30°C in the goldfish cell line RyuF-2. Subsequent in vivo efficacy tests were conducted with vaccination temperatures ranging from 15 to 30°C...
December 19, 2023: Journal of Fish Diseases
Xi Chen, Hua Chen, Jun-Qing Ge
Eel (Anguilla sp.) is an important freshwater-cultured species with high economic value in China. Anguillid herpesvirus 1 (AngHV-1) has been proven to be the pathogen of "mucus sloughing and haemorrhagic septicaemia disease" in eels, resulting in significant mortality and substantial losses to the eel industry. Current diagnostic methods for detecting AngHV-1 are limited to laboratory-based tests, for example, conventional end-point PCR and qPCR. Therefore, there is an urgent need to develop an accurate, rapid, and simple detection method for on-site diagnosis of AngHV-1...
December 19, 2023: Journal of Fish Diseases
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