Ehdaa Eltayeb Eltigani Abdelsalam, Zuzana Bláhová, Ali Asghar Baloch, Veronika Piačková
Carp oedema virus (CEV) has distinct molecularly identified genogroups of viral mutations, denoted as I, IIa, and IIb. Failure to propagate CEV in vitro limits studies towards understanding its interactions with host cells. Here, virus isolates belonging to genogroup I collected during natural outbreaks in the Czech Republic were employed for routine CEV cultivation in monolayers of carp-derived primary cells, common carp brain (CCB) cells, and epithelioma papulosum cyprinid (EPC) cells. Induction of cytopathic effects (CPEs) was observed and recorded in affected cells...
February 29, 2024: Journal of Fish Diseases
Yingyue Yu, Songyue You, Chengcheng Feng, Xiaodong Li, Yuenan Xing, Qingbiao Hu, Jie Bao, Hongbo Jiang
During breeding, some oriental river prawns (Macrobrachium nipponense, de Haan), an important aquaculture species in China, exhibit yellowish-brown body colouration, reduced appetite, and vitality. Diseased prawns revealed characteristic emulsifying disease signs, including whitened musculature, hepatopancreatic tissues, milky haemolymph, and non-coagulation. The present study investigated the causative agent of M. nipponense infection through isolation, histopathology, molecular sequencing, and infection experiments...
February 29, 2024: Journal of Fish Diseases
Thanh-Nguyen Le, Thuan-Thien Dinh, Thuy-Dung Mai-Hoang, Ebrahim Razzazi-Fazeli, Hieu Tran-Van
Acute Hepatopancreatic Necrosis Disease (AHPND) represents a significant challenge in the field of shrimp aquaculture. This disease is primarily caused by Vibrio parahaemolyticus strains harbouring the pVA1 plasmid encoding the PirAvp and PirBvp toxins. To combat this epidemic and mitigate its devastating consequences, it is crucial to identify and characterize the receptors responsible for the binding of these pathogenic toxins. Our studied discovered that Penaeus vannamei's Serine protease inhibitor 3 (PvSerpin3) derived from shrimp hepatopancreatic tissues could bind to recombinant PirAvp , confirming its role as a novel PirAvp -binding protein (PA BP)...
February 25, 2024: Journal of Fish Diseases
Ginevra Brocca, Alessandro Truant, Hana Peckova, Martina Lisnerová, Alberto Perolo, Marialetizia Fioravanti, Ivan Fiala, Gianfranco Gabai, Francesco Quaglio, Andrea Gustinelli
Nodular gill disease (NGD) is an emerging condition associated with amoeba trophozoites in freshwater salmonid farms. However, unambiguous identification of the pathogens still must be achieved. This study aimed to identify the amoeba species involved in periodic NGD outbreaks in two rainbow trout (Oncorhynchus mykiss) farms in Northeastern Italy. During four episodes (February-April 2023), 88 fish were euthanized, and their gills were evaluated by macroscopic, microscopic and histopathological examination...
February 23, 2024: Journal of Fish Diseases
Widanarni Widanarni, Muhamad Gustilatov, Julie Ekasari, Pande Gde Sasmita Julyantoro, Diana Elizabeth Waturangi, Sukenda Sukenda
This study aimed to evaluate and unveil the positive impact of biofloc culture on Vibrio parahaemolyticus infection of Pacific white shrimp by reducing quorum sensing (QS) and virulence gene expression and enhancing shrimp's immunity. The shrimp with an average body weight of 0.50 ± 0.09 g were reared in containers with a volume of 2.5 L, 21 units, and a density of 20 shrimp L-1 . The shrimp were cultured for 5 days, with each treatment including biofloc system maintenance with a C/N ratio of 10 and a control treatment without biofloc, followed by a challenge test through immersion using V...
February 19, 2024: Journal of Fish Diseases
Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
Vibrio harveyi is commonly found in salt and brackish water and is recognized as a serious bacterial pathogen in aquaculture worldwide. In this study, we cloned the ferric uptake regulator (fur) gene from V. harveyi wild-type strain HA_1, which was isolated from diseased American eels (Anguilla rostrata) and has a length of 450 bp, encoding 149 amino acids. Then, a mutant strain, HA_1-Δfur, was constructed through homologous recombination of a suicide plasmid (pCVD442). The HA_1-Δfur mutant exhibited weaker biofilm formation and swarming motility, and 18-fold decrease (5...
February 19, 2024: Journal of Fish Diseases
Xiaoru Liu, Yong Cao, Jiayin Wang, Suyuheng Cao, Liqun Lu, Yousheng Jiang
Large yellow croaker (Larimichthys crocea) is a vital marine-cultured species in China. Large yellow croaker iridovirus (LYCIV) can cause a high mortality rate in L. crocea. Rapid and convenient detection of LYCIV is an urgent demand for diagnosis. In this study, rapid and simple recombinase polymerase amplification (RPA), real-time RPA and RPA combined with lateral flow dipstick (RPA-LFD) methods were developed for the detection of LYCIV based on the conserved sequence of the LYCIV major capsid protein (MCP) gene...
February 13, 2024: Journal of Fish Diseases
Xiao-Meng Guo, Jing-Yi Xing, Anqi Li, Liang Qiu, Qing-Li Zhang, Jie Huang
No abstract text is available yet for this article.
February 1, 2024: Journal of Fish Diseases
Nasmia Nasmia, Novalina Serdiati, Akbar Marzuki Tahya, Muhammad Safir
Vibrio harveyi and Vibrio parahaemolyticus are species of the Vibrio genus that often cause disease and mass mortality in crustaceans. If not handled quickly and appropriately, these diseases can cause considerable losses to farmers. Therefore, it is necessary to find a solution with safe and environmentally friendly disease prevention technology using natural ingredients, among others from plants, namely oil palm. Some parts of oil palm, namely leaves, fronds, fibres and oil palm pulp, which are palm waste, contain antibacterial compounds...
February 1, 2024: Journal of Fish Diseases
Denis V Prazdnikov, Ekaterina A Kondakova
Teratoma is a rare tumour in fish consisting of tissues from more than one germ layer, that may be located in either the gonads or extragonadal sites. Teratomas in many fish species remain poorly understood. In this work, we performed the first histological examinations of extragonadal teratomas in Poecilia wingei and also examined the influence of a large teratoma on male sexual activity. The studied teratomas shared general organizational features, but they also had variations in both external and internal features...
January 30, 2024: Journal of Fish Diseases
A Nafeez Ahmed, S Mithra, S Suryakodi, G Taju, M J Abdul Wazith, K Kanimozhi, V Rajkumar, A Badhusha, S Abdul Majeed, A S Sahul Hameed
A permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub-cultured more than 75 passages and showed optimal growth at 28°C using L-15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial-like cells. The SPB cells were tested for mycoplasma contamination which was found to be negative. The origin of the SPB cell line from T...
January 29, 2024: Journal of Fish Diseases
Sonia A Soto-Rodriguez, Francis I Marrujo Lopez, Karla G Aguilar-Rendon, Rafael Hernández Guzmán
The present study investigates molecular-based PCR techniques to estimate the prevalence of fish pathogens in southwest Mexico where recurrent mortality in the tilapia cultures has been observed. Sample of internal organs and lesions of Nile tilapia were taken and analysed in 2018, 2019, 2020 and 2022 to detect bacterial pathogens using PCR. No samples were taken in 2021 due to the COVID-19 pandemic. The real-time PCR conditions were optimized to allow a qualitative reliable detection of the bacteria from fixed fish tissue...
January 25, 2024: Journal of Fish Diseases
Hee-Jae Choi, Da-Yeon Choi, Ji-Hoon Lee, Jun-Hwan Kim, Yue Jai Kang
No abstract text is available yet for this article.
January 24, 2024: Journal of Fish Diseases
Irene Maja Nanni, Perla Tedesco, David Baldo, Roberta Galuppi, Marina Collina
No abstract text is available yet for this article.
January 24, 2024: Journal of Fish Diseases
Alexandra K Mason, Lance Adams, Michael M Garner
Sixteen whitespotted bamboo sharks (Chiloscyllium plagiosum) with histologically similar bacterial abscesses were submitted to Northwest ZooPath from nine zoo and aquarium institutions over a 17-year period. These abscesses were characterized by inflammatory cell infiltrates and necrosis with intralesional small, Gram-positive, acid-fast negative, cocci bacteria. The clinical presentation, histologic findings, and culture results indicate that Enterococcus faecalis is a relatively common cause of these lesions in whitespotted bamboo sharks...
January 19, 2024: Journal of Fish Diseases
Fanbin Zhan, Shichun Zhou, Fei Shi, Qingqing Li, Li Lin, Zhendong Qin
The phosphoinositide-3-kinase/protein kinase b (PI3K-Akt) pathway is a signalling pathway based on protein phosphorylation and can be activated by a wide range of factors. To investigate the function of the PI3K-AKT signalling pathway in antibacterial immunity, we analysed the gene expression level of three key factors (PI3K, AKT and FoxO) and innate immune factors in immune tissues at different time points after Vibrio parahaemolyticus and Staphylococcus aureus infection. Tissues analysis showed that PI3K, AKT, and FoxO were expressed at high levels in the intestinal, hemocytes and hepatopancreas...
February 2024: Journal of Fish Diseases
Haakon Hansen, Bjørn Spilsberg, Sigmund Sevatdal, Trine Sakariassen, Christoph Hahn, Saima Nasrin Mohammad, Egil Karlsbakk
Detection of intestinal parasites in fish typically requires autopsy, resulting in the sacrifice of the fish. Here, we describe a non-lethal method for detecting the tapeworm Eubothrium crassum in fish using anal swabs and real-time PCR detection. Two assays were developed to detect cytochrome oxidase I (COI) mitochondrial DNA and 18S ribosomal DNA sequences of E. crassum, respectively. The assays were tested on swab samples from confirmed pathogen free Atlantic salmon (Salmo salar L.) and on samples from farmed Atlantic salmon, where the presence and intensity of parasites had been established through autopsy...
January 18, 2024: Journal of Fish Diseases
John Brian Jones, Cara L Brosnahan, Anjali Pande
Lobsters and crayfish in Australasia can develop a condition known as Tail Fan Necrosis (TFN syndrome). Many attempts have been made to find a primary pathogen or link the syndrome to commercial activities, but a solution remains elusive. TFN syndrome is a 'wicked problem', a problem difficult or impossible to solve because of incomplete and contradictory information forming a matrix of potential outcomes with no simple solution. Reviewing the literature shows TFN syndrome is sometimes reported to develop in association with sterile blisters on the telson and uropods which may rupture permitting invasion by environmental fungal and/or bacterial flora...
January 16, 2024: Journal of Fish Diseases
Srirengaraj Vijayaram, Hary Razafindralambo, Yun Zhang Sun, Giuseppe Piccione, Cristiana Roberta Multisanti, Caterina Faggio
Nanotechnology is an expanding and new technology that prompts production with nanoparticle-based (1-100 nm) organic and inorganic materials. Such a tool has an imperative function in different sectors like bioengineering, pharmaceuticals, electronics, energy, nuclear energy, and fuel, and its applications are helpful for human, animal, plant, and environmental health. In exacting, the nanoparticles are synthesized by top-down and bottom-up approaches through different techniques such as chemical, physical, and biological progress...
January 16, 2024: Journal of Fish Diseases
Mengmeng Chen, Mingyang Xue, Jingtao Chen, Zidong Xiao, Xiaowei Hu, Chunjie Zhang, Nan Jiang, Yuding Fan, Yan Meng, Yong Zhou
Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle (Pelodiscus sinensis), then named strain JDP-FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature-sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP-FX...
January 13, 2024: Journal of Fish Diseases
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