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Journal of Fish Diseases

Nathiga Nambi Kalaiselvi Sivalingam, Abdul Majeed Seepoo, Taju Gani, Sivakumar Selvam, Sahul Hameed Azeez Sait
The goal of this study was to develop and characterize a cell line from the caudal fin tissue of zebrafish and also its application as an in vitro model to study the effect of H2 O2 in wound healing. Fibroblastic cell line was developed using explant culture method from caudal fin tissue of zebrafish and characterized. This cell line was named as DrF cell line. The DrF cells treated with 0-10 µM/ml H2 O2 were tested for viability, proliferation and motility by MTT assay, trypan blue assay and chemotaxis assay, respectively...
February 14, 2019: Journal of Fish Diseases
Yolanda Torres-Corral, Ysabel Santos
This study reports on the characterization of Vagococcus salmoninarum using phenotypic, serological, antigenic, genetic and proteomic methods. All strains of V. salmoninarum were resistant to most of the antimicrobials tested, and only 10% of strains were sensitive to florfenicol. Serological analysis demonstrated a high antigenic homogeneity within the species. No cross-reaction was detected with other fish pathogenic species causing streptococcosis (Lactococcus garvieae, Streptococcus parauberis, Streptococcus iniae, Streptococcus agalactiae, Carnobacterium maltaromaticum) using serum against V...
February 11, 2019: Journal of Fish Diseases
Noorashikin Md Noor, Tom Defoirdt, Norfarrah Alipiah, Murni Karim, Hassan Daud, Ikhsan Natrah
The link between quorum sensing in Vibrio campbellii and its virulence towards tiger grouper (Epinephelus fuscoguttatus) was investigated using V. campbellii wild type and quorum-sensing mutants with inactive quorum sensing or constitutively maximal quorum-sensing activity, and signal molecule synthase mutants. The results showed that wild-type V. campbellii is pathogenic to grouper larvae, causing more than 50% mortality after 4 days of challenge. Furthermore, the mortality of larvae challenged with the mutant with maximally active quorum sensing was significantly higher than that of larvae challenged with the wild type, whereas a higher survival was observed in the larvae challenged to the mutant with a completely inactive quorum-sensing system...
February 11, 2019: Journal of Fish Diseases
Nan Jiang, Xiaoyi Pan, Zemao Gu, Wenzhi Liu, Kaige Si, Yong Zhou, Yongze Zhou, Liwen Zhai, Yuding Fan, Lingbing Zeng
The replication profile of white spot syndrome virus (WSSV) in crayfish, Procambarus clarkii, at different water temperature was investigated in this study. The WSSV detections were negative at 15 ± 1°C, and the natural infection ratio increased at 19 ± 1°C (24.2% ± 2.25%), reached 100% at 25 ± 1°C and decreased at 30 ± 1°C (93.2% ± 3.37%). The WSSV genome copies number was much higher at 25 ± 1°C (≥5 × 106.45 ± 0.35 /mg) than at 15 ± 1°C (≤5 × 101.13 ± 0.12 /mg), 19 ± 1°C (≤5 × 102...
February 11, 2019: Journal of Fish Diseases
Krishna K Thakur, Raphaël Vanderstichel, Karia Kaukinen, Omid Nekouei, Emilie Laurin, Kristina M Miller
In response to concerns that novel infectious agents were introduced through the movement of eggs as Atlantic salmon aquaculture developed in British Columbia (BC), Canada, we estimated the prevalence of infectious agents in archived return-migrating Sockeye salmon, from before and during aquaculture expansion in BC (1985-94). Of 45 infectious agents assessed through molecular assays in 652 samples, 23 (7 bacterial, 2 viral and 14 parasitic) were detected in liver tissue from six regions in BC. Prevalence ranged from 0...
February 11, 2019: Journal of Fish Diseases
Md Javed Foysal, Farhana Momtaz, Md Hazrat Ali, Muhammad A B Siddik, Md Reaz Chaklader, Md Mahbubur Rahman, Md Shamsul Haque Prodhan, Anthony Cole
Aerolysin (aer) is one of the most important and abundant virulence factors in the infection of fish by Aeromonas veronii. A comprehensive study on the molecular characterization and pathogenicity of the aer gene from 34 A. veronii isolates from diseased carp and catfish was carried out and its interactome was analysed to observe the functional correlations between aer and other proteins within the A. veronii network. The PCR-based amplification of aer from the 34 isolates of A. veronii showed more aer-positive isolates from catfish with a high pathogenic potential in the in vivo challenge test than the carp fish...
February 7, 2019: Journal of Fish Diseases
Theeraporn Pulpipat, Kuo-Hua Lin, Ying-Hao Chen, Pei-Chi Wang, Shih-Chu Chen
Francisella noatunensis subsp. orientalis is a causative agent of systemic granulomatous disease in tilapia. The present study was designed to understand the genetic and phenotypic diversities among Taiwanese Fno isolates obtained from tilapia (n = 17) and green Texas cichlid (Herichthys cyanoguttatus) (n = 1). The enzymatic profiles of the isolates were studied using the API ZYM system. Phylogenetic tree analysis of the 16S rRNA and housekeeping gene and pulsed-field gel electrophoresis (PFGE) were carried out to determine the genotypic characters of all isolates...
February 4, 2019: Journal of Fish Diseases
Chen Li, Yanbei Ren, Xuan Dong, Chongming Wang, Jie Huang
No abstract text is available yet for this article.
February 4, 2019: Journal of Fish Diseases
Somayeh Torabi Delshad, Siyavash Soltanian, Hassan Sharifiyazdi, Peter Bossier
In this study, we evaluated the impact of the catecholamines on growth, swimming motility, biofilm formation and some virulence factors activities of pathogenic Yersinia ruckeri. Norepinephrine and dopamine (at 100 µM) significantly increased the growth of Y. ruckeri in culture media containing serum. An increase in swimming motility of the pathogen was found following the exposure to the hormones; however, no effect was seen on caseinase, phospholipase and haemolysin productions. Further, antagonists for the catecholamine receptors were observed to block some of the influences of the catecholamines...
January 29, 2019: Journal of Fish Diseases
Young Chul Kim, Woo Ju Kwon, Joon Gyu Min, Kwang Il Kim, Hyun Do Jeong
We determined the complete genomic RNA sequence of a new type of betanodavirus Korea shellfish nervous necrosis virus (KSNNV) isolated from shellfish. Compared with other isolates representing four genotypes of betanodaviruses, the identity of the whole nucleotide sequence of the virus was in the range of 76%-83% with the presence of specific genetic motifs and formed a separate new branch in the phylogenetic analysis. In pathogenic analysis by immersion method, KSNNV-KOR1 shows 100% cumulative mortality like SFRG10/2012BGGa1 (RGNNV) in newly hatched sevenband grouper and mandarin fish, which is clearly different from those found in negative control groups...
January 29, 2019: Journal of Fish Diseases
Héctor A Levipan, Rute Irgang, Diana Tapia-Cammas, Ruben Avendaño-Herrera
No abstract text is available yet for this article.
January 21, 2019: Journal of Fish Diseases
Wenjing Hao, Qi Gao, Jian Wang, Wei Gu, Wen Wang, Qingguo Meng
No abstract text is available yet for this article.
January 18, 2019: Journal of Fish Diseases
S Thamizhvanan, S Sivakumar, S Santhosh Kumar, D Vinoth Kumar, S Suryakodi, K Balaji, T Rajkumar, S Vimal, S Abdul Majeed, G Taju, A S Sahul Hameed
White leg shrimp, Penaeus vannamei, were collected on a monthly basis from grow-out ponds located at Tamil Nadu and Andhra Pradesh states along the east coast of India for screening of viral and other pathogens. Totally 240 shrimp samples randomly collected from 92 farms were screened for white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV), infectious myonecrosis virus (IMNV) and Enterocytozoon hepatopenaei (EHP). The number of shrimp collected from shrimp farms ranged from 6 to 20 based on the body weight of the shrimp...
January 18, 2019: Journal of Fish Diseases
Yangxi Xiang, Peng Jia, Wei Liu, Meisheng Yi, Kuntong Jia
Nervous necrosis virus (NNV) is one of the fish pathogens that have caused mass mortalities of many marine and freshwater fishes in the world. To better comprehend the molecular immune mechanism of sea perch (Lateolabrax japonicus) against NNV infection, the comparative transcriptome analysis of red-spotted grouper nervous necrosis virus (RGNNV)-infected or mock-infected L. japonicus brain (LJB) cells was performed via RNA sequencing technology. Here, 1,969 up-regulated genes and 9,858 down-regulated genes, which were widely implicated in immune response pathways, were identified...
January 18, 2019: Journal of Fish Diseases
Åse Helen Garseth, Torfinn Moldal, Siri Kristine Gåsnes, Monika Jankowska Hjortaas, Vegard Pedersen Sollien, Anne-Gerd Gjevre
In 2017, a PCR-based survey for Piscine orthoreovirus-3 (PRV-3) was conducted in wild anadromous and non-anadromous salmonids in Norway. In seatrout (anadromous Salmo trutta L.), the virus was present in 16.6% of the fish and in 15 of 21 investigated rivers. Four of 221 (1.8%) Atlantic salmon (Salmo salar L.) from three of 15 rivers were also PCR-positive, with Ct-values indicating low amounts of viral RNA. All anadromous Arctic char (Salvelinus alpinus L.) were PCR-negative. Neither non-anadromous trout (brown trout) nor landlocked salmon were PRV-3 positive...
January 18, 2019: Journal of Fish Diseases
Carliane Maria Guimarães Alves, Joziele Neves Nogueira, Isaac Belo Barriga, Joilson Rodrigues Dos Santos, Gracienhe Gomes Santos, Marcos Tavares-Dias
This study evaluated the efficacy of albendazole, ivermectin, levamisole, mebendazole and praziquantel on monogeneans of Colossoma macropomum, based on in vitro and in vivo assays. In vitro assays indicated that albendazole (500, 100, 1,500 and 2,000 mg/L), ivermectin (200, 250, 300 and 350 mg/L) and levamisole (50, 75, 100 and 125 mg/L) were 100% effective against Anacanthorus spatulatus, Notozothecium janauachensis, Mymarothecium boegeri and Linguadactyloides brinkmanni, while mebendazole (125, 150, 175 and 200 mg/L) and praziquantel (5, 10, 15 and 20 mg/L) were ineffective...
January 18, 2019: Journal of Fish Diseases
Ana C Luz Cunha, Vitor de A Pontinha, Maria Alcina M de Castro, Simone Sühnel, Sthefanie C Medeiros, Ângela M Moura da Luz, Ricardo Harakava, Leonardo Tachibana, Danielle F Mello, Naissa M Danielli, Alcir L Dafre, Aimê R M Magalhães, José Luiz P Mouriño
Perkinsus spp. have been detected in various bivalve species from north-east Brazil. Santa Catarina is a South Brasil state with the highest national oyster production. Considering the pathogenicity of some Perkinsus spp., a study was carried out to survey perkinsosis in two oyster species cultured in this State, the mangrove oyster Crassostrea gasar and the Pacific oyster Crassostrea gigas. Sampling involved eight sites along the state coast, and oyster sampling was collected during the period between January 2013 and December 2014...
January 18, 2019: Journal of Fish Diseases
Lixing Huang, Yanfei Zuo, Qingling Jiang, Yongquan Su, Yingxue Qin, Xiaojin Xu, Lingmin Zhao, Qingpi Yan
Pseudomonas plecoglossicida is associated with multiple fish diseases, and temperature is one of the most important environmental factors related to its outbreak. To elucidate the influence of temperature variation on the pathogen, the global metabolomics of P. plecoglossicida (NZBD9) were analysed at the virulent (18°C) and avirulent (12°C and 28°C) temperatures. The result showed that the levels of Phosphoric acid, Tyrosine, Spermidine and Sucrose were significantly reduced,while Itaconic acid, Glucaric acid and Isomaltose were increased in P...
January 18, 2019: Journal of Fish Diseases
Jorge Mancilla-Schulz, Sandra L Marín, Carlos Molinet
Caligus rogercresseyi generates the greatest losses in the salmon industry in Chile. The relationship between salmon farming and sea lice is made up of various components: the parasite, host, environment and farming practices, which make it difficult to identify patterns in parasite population dynamics to define prevention and control strategies. The objectives of this study were to analyse and compare the effect of farming, sanitary practices and environmental variables on the abundance of gravid females (GF) and juveniles (JUV) of C...
January 15, 2019: Journal of Fish Diseases
Benjamin H Beck, Mediha Yildirim-Aksoy, Craig A Shoemaker, Sidney Adam Fuller, Eric Peatman
The antimicrobial activity and mode of action of chitosan were evaluated against Streptococcus iniae, a pathogenic Gram-positive bacterium of fish worldwide. Cell proliferation kinetics were examined following exposure to varying concentrations of chitosan. The action of chitosan on S. iniae was also investigated by measuring agglutination activity, conductivity, and extracellular and intracellular bacterial adenosine triphosphate (ATP) levels. Chitosan exhibited antibacterial activity against S. iniae at concentrations of 0...
January 15, 2019: Journal of Fish Diseases
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