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Plant Molecular Biology Reporter

Hang Yu, Muhammad Qasim Shahid, Rongbai Li, Wei Li, Wen Liu, Fozia Ghouri, Xiangdong Liu
Common wild rice ( Oryza rufipogon Griff.) is invaluable genetic resource for rice resistance breeding. Whole-genome re-sequencing was conducted to systematically analyze the variations in two new inbred lines (Huaye 3 and Huaye 4) developed from a common wild rice. A total of 4,841,127 SNPs, 1,170,479 InDels, 24,080 structural variations (SVs), and 298 copy number variations (CNVs) were identified in three materials. Approximately 16.24 and 5.64% of the total SNPs and InDels of Huaye 3 and Huaye 4 were located in genic regions, respectively...
2018: Plant Molecular Biology Reporter
Xiaohua Wang, Ping Xu, Liang Yin, Yan Ren, Shuangling Li, Yanmao Shi, Thomas D Alcock, Qing Xiong, Wei Qian, Xiaoyuan Chi, Manish K Pandey, Rajeev K Varshney, Mei Yuan
Peanut ( Arachis hypogaea ), a major source of vegetable oil in many Asian countries, has become an integral part of human diet globally due to its high nutritional properties and option to consume in different forms. In order to meet the demand of vegetable oil, many peanut breeding programs of China have intensified their efforts in increasing oil content in newly bred varieties for reducing the import of edible oils in China. In this context, transcriptome sequencing data generated on 49 peanut cultivars were analyzed to identify candidate genes and develop molecular markers for seed oil content across multiple environments...
2018: Plant Molecular Biology Reporter
R Daneshkhah, F M W Grundler, Krzysztof Wieczorek
Serendipita indica is an axenically cultivable fungus, which colonizes a broad range of plant species including the model plant Arabidopsis thaliana . Root colonization by this endophyte leads to enhanced plant fitness and performance and promotes resistance against different biotic and abiotic stresses. The involvement of MPK6 in this mutualistic interaction had been previously shown with an mpk6 A. thaliana mutant, which failed to respond to S. indica colonization. Here, we demonstrate that mpk6 roots are significantly less colonized by S...
2018: Plant Molecular Biology Reporter
Matthew J Christians, Aron Rottier, Carly Wiersma
In plants, the small protein related to ubiquitin (RUB) modifies cullin (CUL) proteins in ubiquitin E3 ligases to allow for efficient transfer of ubiquitin to substrate proteins for degradation by the 26S proteasome. At the molecular level, the conjugation of RUB to individual CUL proteins is transient in nature, which aids in the stability of the cullins and adaptor proteins. Many changes in cellular processes occur within the plant upon exposure to light, including well-documented changes in the stability of individual proteins...
2018: Plant Molecular Biology Reporter
Yi Ming Guo, Birgit Samans, Sheng Chen, Kidist B Kibret, Sarah Hatzig, Neil C Turner, Matthew N Nelson, Wallace A Cowling, Rod J Snowdon
Production of oilseed rape/canola ( Brassica napus ) is increasingly threatened by dry conditions while the demand for vegetable oil is increasing. Brassica rapa is a genetically diverse ancestor of B. napus , and is readily crossed with B. napus . Recently, we reported promising levels of drought tolerance in a wild type of B. rapa which could be a source of drought tolerance for B. napus . We analysed global gene expression by messenger RNA sequencing in seedlings of the drought-tolerant and a drought-sensitive genotype of B...
2017: Plant Molecular Biology Reporter
Aliz Novák, Ákos Boldizsár, Krisztián Gierczik, Attila Vágújfalvi, Éva Ádám, László Kozma-Bognár, Gábor Galiba
The wheat and barley CBF14 genes have been newly defined as key components of the light quality-dependent regulation of the freezing tolerance by the integration of phytochrome-mediated light and temperature signals. To further investigate the wavelength dependence of light-induced CBF14 expression in cereals, we carried out a detailed study using monochromatic light treatments at an inductive and a non-inductive temperature. Transcript levels of CBF14 gene in winter wheat Cheyenne, winter einkorn G3116 and winter barley Nure genotypes were monitored...
2017: Plant Molecular Biology Reporter
Jie Zhang, Kai Zhao, Dan Hou, Junhuo Cai, Qixiang Zhang, Tangren Cheng, Huitang Pan, Weiru Yang
Next-generation sequencing technologies provide opportunities to ascertain the genetic basis of phenotypic differences, even in the closely related cultivars via detection of large amount of DNA polymorphisms. In this study, we performed whole-genome re-sequencing of two mei cultivars with contrasting tree architecture. 75.87 million 100 bp pair-end reads were generated, with 92 % coverage of the genome. Re-sequencing data of two former upright mei cultivars were applied for detecting DNA polymorphisms, since we were more interested in variations conferring weeping trait...
2017: Plant Molecular Biology Reporter
Feifei An, Genghu Li, Qing X Li, Kaimian Li, Luiz J C B Carvalho, Wenjun Ou, Songbi Chen
Cassava ( Manihot esculenta Crantz) is a tropical root crop and sensitive to low temperature. However, it is poorly to know how cassava can modify its metabolism and growth to adapt to cold stress. An investigation aimed at a better understanding of cold-tolerant mechanism of cassava plantlets was carried out with the approaches of physiology and proteomics in the present study. The principal component analysis of seven physiological characteristics showed that electrolyte leakage (EL), chlorophyll content, and malondialdehyde (MDA) may be the most important physiological indexes for determining cold-resistant abilities of cassava...
2016: Plant Molecular Biology Reporter
Adam Rajsz, Anna Warzybok, Magdalena Migocka
Full-size members of the ABCG (ATP-binding cassette, subfamily G) subfamily of ABC transporters have been found only in plants and fungi. The plant genes encoding full-size ABCGs identified so far appeared to be differentially regulated under various environmental constraints, plant growth regulators, and microbial elicitors, indicating a broad functional role of these proteins in plant responses to abiotic and biotic stress. Nevertheless, the structure and physiological function of full-size ABCGs in many plant species are still unknown...
2016: Plant Molecular Biology Reporter
Xiao-Fang Zeng, De-Gang Zhao
The IPT gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. We introduced IPT under the control of the CaMV35S promoter into Asakura-sanshoo (Zanthoxylum piperitum (L.) DC. f. inerme Makino) via stable Agrobacterium tumefaciens-mediated transformation. Three of 3-year-old transgenic Asakura-sanshoo lines Y5, Y16, and Y17 were used to evaluate the effects of IPT expression on the morphological characteristics, leaf senescence, and essential oil composition. Introduced IPT into Asakura-sanshoo stimulated an increase in cytokinin content and a decrease in auxin level...
2016: Plant Molecular Biology Reporter
Tongbing Su, Shuancang Yu, Ruifang Yu, Fenglan Zhang, Yangjun Yu, Deshuang Zhang, Xiuyun Zhao, Weihong Wang
By cultivating tipburn-susceptible plants in modified Hoagland's medium containing of gradient exogenous calcium (Ca(2+)), we have shown that Ca(2+) deficiency is one of the main causes of tipburn in Chinese cabbage (Brassica rapa L. ssp. pekinensis). The effect of endogenous plant Ca(2+) concentrations on tipburn was also studied in a doubled haploid (DH) population consisting of 100 individuals, but no correlation was found. We then examined the expression of 12 Ca(2+) transporter genes that function in cytosolic Ca(2+) homeostasis in both tipburn-susceptible and tipburn-resistant plants under normal and tipburn-inducing conditions...
2016: Plant Molecular Biology Reporter
Tao Yang, Xing Liu
Isoetes L. is an ancient genus of heterosporous lycopsids with a unique phylogenetic position. Repeated adaptations to environmental changes over time have contributed to occupying a variety of niches in Isoetes. However, we know little about how they adapt to the environmental changes, and the sequence resources are very limited in public databases. Isoetes sinensis is an amphibious plant in this genus, alternating frequently between terrestrial and aquatic environments. In this study, I. sinensis was applied to investigate the adaptations under terrestrial (TC) and submerged (ST) conditions using Illumina RNA-sequencing technology...
2016: Plant Molecular Biology Reporter
M Targońska, H Bolibok-Brągoszewska, M Rakoczy-Trojanowska
The primary aim of this study was to estimate genetic diversity among Secale cereale L. accessions using 22 previously published simple sequence repeat (SSR) markers. The plant material included 367 rye accessions comprising historical and contemporary cultivars, cultivated materials, landraces, and breeding strains from the Polish breeding company Danko. The studied accessions represented a wide geographical diversity. Several methods were employed to analyze genetic diversity among the Secale cereale L. accessions and to determine population structure: principal coordinate analysis (PCoA), neighbor-joining (NJ), and Bayesian clustering...
2016: Plant Molecular Biology Reporter
Samir Kumar Gunjan, Dennis Trent Rogers, Jingxian Zhang, Kil-Young Yun, Deane L Falcone, John Littleton
Cell-based screening methods for nuclear receptor ligands that use transgenic plant cells expressing a single human NR may have advantages over other eukaryotic systems which express multiple NRs. For example, signal-to-noise ratio might be improved because ligands would be less likely to bind to other NRs and/or less likely to cause confounding functional changes in plant cells. As a first step toward this aim we have expressed in plants truncated human estrogen receptor (ER) constructs linked to reporters, or selective markers such as luciferase, green fluorescent protein (GFP) and hygromycin...
December 1, 2015: Plant Molecular Biology Reporter
Tomasz L Mróz, Michael J Havey, Grzegorz Bartoszewski
Alternative oxidase (AOX) is a mitochondrial terminal oxidase which is responsible for an alternative route of electron transport in the respiratory chain. This nuclear-encoded enzyme is involved in a major path of survival under adverse conditions by transfer of electrons from ubiquinol instead of the main cytochrome pathway. AOX protects against unexpected inhibition of the cytochrome c oxidase pathway and plays an important role in stress tolerance. Two AOX subfamilies (AOX1 and AOX2) exist in higher plants and are usually encoded by small gene families...
2015: Plant Molecular Biology Reporter
Ana C M Brasileiro, Carolina V Morgante, Ana C G Araujo, Soraya C M Leal-Bertioli, Amanda K Silva, Andressa C Q Martins, Christina C Vinson, Candice M R Santos, Orzenil Bonfim, Roberto C Togawa, Mario A P Saraiva, David J Bertioli, Patricia M Guimaraes
Peanut (Arachis hypogaea L.) is an important legume cultivated mostly in drought-prone areas where its productivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for peanut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil...
2015: Plant Molecular Biology Reporter
Ariel Herrera-Vásquez, Loreto Carvallo, Francisca Blanco, Mariola Tobar, Eva Villarroel-Candia, Jesús Vicente-Carbajosa, Paula Salinas, Loreto Holuigue
Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition...
2015: Plant Molecular Biology Reporter
M Awais Khan, David Saravia, Susan Munive, Flavio Lozano, Evelyn Farfan, Raul Eyzaguirre, Merideth Bonierbale
Dissection of the genetic architecture of adaptation and abiotic stress-related traits is highly desirable for developing drought-tolerant potatoes and enhancing the resilience of existing cultivars, particularly as agricultural production in rain-fed areas may be reduced by up to 50 % by 2020. The "DMDD" potato progeny was developed at International Potato Center (CIP) by crossing the sequenced double monoploid line DM and a diploid cultivar of the Solanum tuberosum diploid Andigenum Goniocalyx group. Recently, a high-density integrated genetic map based on single nucleotide polymorphism (SNP), diversity array technology (DArT), simple sequence repeats (SSRs), and amplified fragment length polymorphism (AFLP) markers was also made available for this population...
2015: Plant Molecular Biology Reporter
Mariangela Coppola, Giandomenico Corrado, Valentina Coppola, Pasquale Cascone, Rosanna Martinelli, Maria Cristina Digilio, Francesco Pennacchio, Rosa Rao
Systemin is a signal peptide that promotes the response to wounding and herbivore attack in tomato. This 18-amino acid peptide is released from a larger precursor, prosystemin. To study the role of systemin as a modulator of defense signaling, we generated tomato (Solanum lycopersicum) transgenic plants that overexpress the prosystemin cDNA. We carried out a transcriptomic analysis comparing two different transgenic events with the untransformed control. The Gene Ontology categories of the 503 differentially expressed genes indicated that several biological functions were affected...
2015: Plant Molecular Biology Reporter
Sara Cregeen, Sebastjan Radisek, Stanislav Mandelc, Boris Turk, Natasa Stajner, Jernej Jakse, Branka Javornik
Verticillium wilt has become a serious threat to hop production in Europe due to outbreaks of lethal wilt caused by a highly virulent strain of Verticillium albo-atrum. In order to enhance our understanding of resistance mechanisms, the fungal colonization patterns and interactions of resistant and susceptible hop cultivars infected with V. albo-atrum were analysed in time course experiments. Quantification of fungal DNA showed marked differences in spatial and temporal fungal colonization patterns in the two cultivars...
2015: Plant Molecular Biology Reporter
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