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Journal of Gene Medicine

Ayman Abd-Elrahman M N, Azza H Abd-El Fatah, Hanan S Ahmed
INTRODUCTION: A common polymorphism (677C to T; Ala to Val) in the methylenetetrahydrofolate reductase (MTHFR) gene is associated with decreased specific MTHFR activity and elevation of the homocysteine. AIM: to investigate the association between single nucleotide polymorphism (SNP) in MTHFR 677C>T gene and insulin resistance in women with polycystic ovary syndrome (PCOS). Subject& Methods Two-hundred patients with PCOS were included in this case- control study...
February 11, 2019: Journal of Gene Medicine
Desheng Sun, Jieyu Zhong, Wei Wei, Xiangmei Chen, Jun Liu, Zhengming Hu
PURPOSE: Sentinel lymph node (SLN) property assessment (with or without metastasis) is important while deciding the surgery for breast cancer; however, the current diagnosis of SLN metastasis remains to be studied. microRNAs (miRNAs) have been previously confirmed as a molecular marker for the diagnosis, development, and prognosis of tumors. However, the detailed role of miRNAs in the diagnosis of SLN metastasis has not been reported. METHODS: In this study, to explore the potential use of miRNAs in the diagnosis of SLN, RNA sequencing (RNA-seq) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to compare the expression profiles of miRNAs in patients with breast cancer with or without SLN metastasis...
February 4, 2019: Journal of Gene Medicine
Lina Zhang, Yanfei Cao, Xiaonan Kou, Lu Che, Xiaona Zhou, Gang Chen, Jiang Zhao
PURPOSE: Glioma is a kind of malignant tumor which occurs in the central nervous system of adults. Long non-coding RNAs (lncRNAs) that potentially participate in the initiation and progression of glioma have been widely reported. As a now-found lncRNA, HLA complex group 11 (HCG11) has not been studied in glioma. The aim of this study is to determine the role of HCG11 in the tumorigenesis of glioma. METHOD: Quantitative real time polymerase chain reaction (qRT-PCR) assay was performed to examine the expression pattern of HCG11 in 84 glioma tissues and cell lines...
February 1, 2019: Journal of Gene Medicine
Liang-Liang Fan, Ji-Shi Liu, Hao Huang, Ran Du, Rong Xiang
BACKGROUND: Hereditary spherocytosis (HS) is an inherited disorder of erythrocyte. The typical feature of HS is the presence of spherical-shaped erythrocytes on the peripheral blood smear. According to previous studies, more than five candidate genes such as ANK1, SPTB, SPTA1, SLC4A1, and EPB42 have been identified in HS patients. METHODS: In this study, we have collected a Chinese HS family. The proband suffered from pathologic jaundice and splenomegaly. Blood test and peripheral blood smear experiment further confirmed the diagnosis of HS...
January 28, 2019: Journal of Gene Medicine
Yongchao Yang, Yu Xia, Yueheng Wu, Shufang Huang, Yun Teng, Xiaobing Liu, Ping Li, Jimei Chen, Jian Zhuang
INTRODUCTION: Cardiac septal defects account for more than 50% of congenital heart defects. Ankyrin repeat domain 1 (ANKRD1) is an important transcription factor that is mutated in multiple cardiac diseases; however, a relationship between ANKRD1 mutation and cardiac septal defects has not been described. METHODS: We examined genetic mutations in a large family with three cardiac septal defect patients. Whole exome sequencing, bioinformatics and conservation analysis were utilized to predict the pathogenicity of candidate mutations...
January 18, 2019: Journal of Gene Medicine
Shuo Yang, Rui-Xing Yin, Liu Miao, Qing-Hui Zhang, Yong-Gang Zhou, Jie Wu
BACKGROUD: Maonan nationality is a relatively isolated minority in China. Little is known about the endothelial lipase gene (LIPG) single nucleotide polymorphisms (SNPs) and serum lipid levels in the Chinese populations. The present study was undertaken to detect the association of several LIPG SNPs and environmental factors with serum lipid levels in the Chinese Maonan and Han populations. METHODS: A total of 773 subjects of Maonan nationality and 710 participants of Han nationality were randomly selected from our previous stratified randomized samples...
January 18, 2019: Journal of Gene Medicine
Guozhu Yin, Hanghang Liu, Jiayang Li, Yao Liu, Xian Liu, En Luo
BACKGROUND: Adiponectin (APN) has been reported to promote bone formation. However, it is difficult to utilize conventional method to administer sufficient APN to the implant site. This study investigated the efficacy of APN transgene to accelerate the implant osseointegration in ovariectomized (OVX) rats. METHODS: In vitro, bone marrow stromal cells (BMSCs) were transduced with reconstructed adenovirus (Ad-APN-EGFP) and osteoclast precursor RAW264.7 cells were co-cultured with the conditioned medium secreted by transduced BMSCs...
January 3, 2019: Journal of Gene Medicine
Shu Uin Gan, Zhenying Fu, Kian Chuan Sia, Oi Lian Kon, Roy Calne, Kok Onn Lee
BACKGROUND: Diabetes mellitus is caused by a partial or complete lack of insulin production in the body. We have previously shown that a single injection of an adeno- associated virus serotype 8 (AAV8) vector carrying a modified and codon optimized human insulin gene induced hepatic production of insulin and corrected streptozotocin (STZ)-induced diabetes in mice for over one year. Insulin production was constitutive, analogous to long-acting insulin therapy. METHODS: We have developed a single AAV8 vector with a Tet-Off regulatable system as a safety mechanism to turn off insulin secretion should hypoglycaemia develop in vector-treated diabetic mice...
December 28, 2018: Journal of Gene Medicine
Giridhara R Jayandharan
No abstract text is available yet for this article.
December 28, 2018: Journal of Gene Medicine
Zhaoliang Lu, Yufen Xu, Lanyan Fu, Yaqian Tan, Di Che, Ping Huang, Lei Pi, Huazhong Zhou, Xiaoyun Liang, Li Zhang, Xiaoqiong Gu
INTRODUCTION: Activated-platelet increases the risk of thrombosis in Kawasaki disease (KD) patients with coronary artery aneurysm (CAA). The ADP pathway is one of the platelet activation and aggregation pathways. The P2RY12 gene encodes the ADP receptor highly concentrated on platelets. However few articles reported P2RY12 relation to KD susceptibility with or without CAA. AIMS: We recruited 1335 healthy controls and 776 KD patients, including 103 with CAA, selected five P2RY12 polymorphisms with SAS statistical analysis research: rs9859538, rs1491974, rs7637803, rs6809699 and rs2046934...
December 21, 2018: Journal of Gene Medicine
Jaqueline Carvalho de Oliveira, Luana Caroline Oliveira, Carolina Mathias, Gabrielle Araújo Pedroso, Debora Souza Lemos, Amanda Salviano-Silva, Tayana Schultz Jucoski, Sara Cristina Lobo-Alves, Erika Pereira Zambalde, Gabriel Adelman Cipolla, Daniela Fiori Gradia
AIMS: This article aims to review the most well characterized lncRNAs with important roles in hallmarks of cancer, additionally including lncRNAs with higher potential for clinical application. DISCUSSION: LncRNAs are transcripts larger than 200 nucleotides in length that do not appear to have protein-coding potential, although some of those may produce small functional peptides. These transcripts have attracted significant attention of researchers due to their role in genetic regulation including epigenetic, transcriptional, and post-transcriptional regulation, being involved in numerous biological processes as well as being associated with multifactorial diseases, including tumorigenesis...
December 14, 2018: Journal of Gene Medicine
Katsuyuki Hamada, Soichi Takagi, Hajime Kuboshima, Hideaki Shimada, Kazuko Takagi, Toshiaki Yasuoka, Keiichi Matsubara, Yukiko Sassa, Tetsuya Furuya, Kazuhiko Suzuki, Tsuyoshi Uchide, Tetsuya Mizutani, Kenzaburo Tani, Hiroshi Itoh, Takashi Sugiyama
BACKGROUND: A549 carrier cells infected with oncolytic adenovirus can induce complete tumor reduction of subcutaneous ovarian tumors but not intraperitoneal disseminated ovarian tumors. This seems to be due to the insufficient antitumor effect of A549 carrier cells. Therefore, here we cloned a novel carrier cell with the aim of improving antitumor effects. METHODS: Carrier cells infected with oncolytic adenovirus AdE3-midkine with a midkine promoter were cloned by limiting dilution...
December 13, 2018: Journal of Gene Medicine
Katarzyna Skonieczna, Arkadiusz Jawień, Andrzej Marszałek, Tomasz Grzybowski
BACKGROUND: p53 is a tumour suppressor protein that is involved in many cancer-related processes. Growing evidence suggests that p53 also plays an important role in mtDNA maintenance. Somatic mitogenome mutations are frequently observed in colorectal cancer cells. Thus, it was important to verify whether somatic mtDNA changes are associated with TP53 mutational status. METHODS: Here, we analysed the TP53 gene in 67 colorectal cancer patients, for whom mitogenome haplotypes were previously described...
December 10, 2018: Journal of Gene Medicine
Xiaoguang Han, Yong Chen, Yajun Liu, Zhuo Wang, Guoqing Tang, Wei Tian
INTRODUCTION: Spinal cord injury (SCI) is a severe worldwide health problem, and efficacious strategies to properly repair SCI have not yet been developed. Recently, use of gene and cell therapy as alternative treatments for SCI have been proposed as safe and promising strategies. AIMS: This study investigated the therapeutic effects and underlying mechanisms of hypoxia-inducible factor-1α carried in recombinant adenovirus (Adv-HIF-1α) administered immediately following spinal cord injury (SCI) in adult rats...
November 9, 2018: Journal of Gene Medicine
Yipeng Ding, Xiaoman Zhou, Cibing Wu, Quanni Li, Juan Sun, Huan Niu, Daobo Lin, Dingwei Sun, Pingdong Xie, Duoyi Wu, Jie Zhao, Ping He
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a disease characterized by airflow limitation. It is not completely reversible and progressive development. ZNF208 rs8105767 affects telomere length, the impact of telomere on COPD is still controversial. So, we want to explore impact of the ZNF208 gene polymorphism on telomere length and telomere length on the COPD in Hainan Li population. MATERIALS AND METHODS: 270 COPD patients and 288 controls were recruited...
November 5, 2018: Journal of Gene Medicine
Jimeen Yoo, Erik Kohlbrenner, Okkil Kim, Roger J Hajjar, Dongtak Jeong
BACKGROUND: Cardiac gene therapy using the adeno-associated virus serotype 9 vector is widely used due to its efficient transduction. However, the promoters used to drive expression often cause off-target localization. To overcome this, former studies have applied cardiac specific promoters but the expression is debilitated compared to that of ubiquitous promoters. To address these issues in the context of atrial specific gene expression, an enhancer calsequestrin cis-regulatory module 4 (CRM4) and the highly atrial specific promoter sarcolipin were combined to enhance expression and minimize off tissue expression...
November 4, 2018: Journal of Gene Medicine
José Luis Royo, Emilio Alarcón-Martín, Jesús Díaz-Fuentes, Juan D Colmenero, María José Bravo
No abstract text is available yet for this article.
October 2018: Journal of Gene Medicine
Guoxia Ren, Qi Dong, Baojuan Huyan, Pu Jin, Mingwei Chen
BACKGROUND: Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family. However, few studies have reported on the polymorphisms of IL1R1 and IL1R2 in TB patients. METHODS: We investigated nine single-nucleotide polymorphisms (SNPs) in IL1R1 and IL1R2 in 300 TB patients and 300 controls, aiming to evaluate their association with TB risk. Odds ratios and 95% confidence intervals were calculated using unconditional logistic regression after adjusting for age and gender...
October 2018: Journal of Gene Medicine
Nejat Düzgüneş, Jennifer Cheung, Krystyna Konopka
BACKGROUND: Cervical cancer is the third most common cause of cancer in women. The 5-year survival rate in oropharyngeal squamous cell carcinomas is approximately 50% and this rate has not improved in recent decades. These cancers are accessible to direct intervention. We examined the ability of a highly efficient non-viral vector, TransfeX (ATCC, Manassas, VA, USA), to deliver the suicide gene HSV-tk to cervical, oral and pharyngeal cancer cells and to induce cytotoxicity following the administration of the prodrug, ganciclovir...
October 2018: Journal of Gene Medicine
Mahdi Noureddini, Narges Mobasseri, Mohammad Karimian, Mohaddeseh Behjati, Hossein Nikzad
BACKGROUND: The Arg399Gln polymorphism in the X-ray repair cross-complementing group 1 gene (XRCC1) may alter the risk of prostate cancer (PCa). The present study aimed to investigate the association of the XRCC1-Arg399Gln polymorphism with PCa risk in an Iranian population, as followed by a meta-analysis and an in silico analysis. METHODS: In a case-control study, 360 subjects were included (180 men with PCa and 180 healthy controls). XRCC1-Arg399Gln genotyping was performed using the polymerase chain reaction-restriction fragment length polymorphism method...
October 2018: Journal of Gene Medicine
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