Saratram Gopalakrishnan, William Johnson, Miguel A Valderrama-Gomez, Elcin Icten, Jasmine Tat, Michael Ingram, Coral Fung Shek, Pik K Chan, Fabrice Schlegel, Pablo Rolandi, Cleo Kontoravdi, Nathan E Lewis
Metabolism governs cell performance in biomanufacturing, as it fuels growth and productivity. However, even in well-controlled culture systems, metabolism is dynamic, with shifting objectives and resources, thus limiting the predictive capability of mechanistic models for process design and optimization. Here, we present Cellular Objectives and State Modulation In bioreaCtors (COSMIC)-dFBA, a hybrid multi-scale modeling paradigm that accurately predicts cell density, antibody titer, and bioreactor metabolite concentration profiles...
February 20, 2024: Metabolic Engineering