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Journals Journal of Histochemistry and ...

Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society

https://read.qxmd.com/read/38907656/expression-of-fam159b-in-humans-rats-and-mice-a-cross-species-examination
#1
JOURNAL ARTICLE
Anna-Sophia Liselott Beyer, Daniel Kaemmerer, Jörg Sänger, Amelie Lupp
Little is known about the adaptor protein FAM159B. To determine whether FAM159B expression findings in rats or mice can be extrapolated to humans, we compared FAM159B expression in healthy tissue samples from all three species using immunohistochemistry. Despite variations in expression intensity, similar FAM159B expression patterns were observed in most organs across species. The most prominent species difference was noted in pancreatic islets; while FAM159B expression was limited to single cells on the outer edges in mice and rats, it was detectable across entire islets in humans...
June 22, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38836522/the-contribution-of-meckel-s-cartilage-derived-type-ii-collagen-positive-cells-to-the-jawbone-development-and-repair
#2
JOURNAL ARTICLE
Hongli Chen, Yunpeng Ding, Yu Wang, Yao Sun
Jawbones and long bones, despite their shared skeletal lineage, frequently exhibit distinct origins and developmental pathways. Identifying specific progenitor subsets for mandibular osteogenesis remains challenging. Type II collagen is conventionally associated with cartilaginous structures, yet our investigation has identified the presence of type II collagen positive (Col2+ ) cells within the jawbone development and regeneration. The role of Col2+ cells in jawbone morphogenesis and repair has remained enigmatic...
June 5, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38804681/immunohistochemical-expression-and-clinical-significance-of-wwp1-protein-in-nasopharyngeal-cancer
#3
JOURNAL ARTICLE
Huarong Chen, Changya Li, Shengmei He, Junjun Ling, Houyu Zhao, Xianlu Zhuo
Nasopharyngeal carcinoma (NPC) is a common malignant tumor of the head and neck. Its pathogenesis is complicated and needs further investigation. The aim of this study was to investigate the expression and clinical significance of WWP1 in NPC. Bioinformatics approaches were used to evaluate the expression and functions of WWP1 in NPC. WWP1 protein expression was then detected by immunohistochemistry on a tissue microarray in an NPC cohort and its association with clinical features and prognosis was determined...
May 28, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38804525/high-molecular-weight-hyaluronan-administration-inhibits-bone-resorption-and-promotes-bone-formation-in-young-age-osteoporosis-rats
#4
JOURNAL ARTICLE
Toshiyuki Kikuchi, Kazuhiko Udagawa, Yoshihiro Sasazaki
Osteoporosis poses a significant global health concern, affecting both the elderly and young individuals, including athletes. Despite the development of numerous antiosteoporotic drugs, addressing the unique needs of young osteoporosis patients remains challenging. This study focuses on young rats subjected to ovariectomy (OVX) to explore the impact of high-molecular-weight hyaluronan (HA) on preventing OVX-induced osteoporosis. Twenty-four rats underwent OVX, while 12 underwent sham procedures (sham control group)...
May 28, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38752478/dcm-spheroid-morphs-express-pads-and-citrullinated-cytoskeletal-proteins
#5
JOURNAL ARTICLE
Alia Sadiq, Justyna Fert-Bober
During investigating the role of peptidylarginine deiminase (PAD) enzymes in dilated cardiomyopathy (DCM), we observed unique spheroid formation in DCM-myofibroblasts that distinguished them from normal cardiac myofibroblasts. The present study aimed to assess the presence of PADs, the extracellular matrix (ECM), and citrullination in DCM spheroids using immunofluorescence staining and imaging techniques. The results revealed that spheroids derived from DCM-myofibroblasts displayed a more distinctive, tightly packed structure compared with those derived from human cardiac fibroblasts...
May 16, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38733294/new-insights-in-atp-synthesis-as-therapeutic-target-in-cancer-and-angiogenic-ocular-diseases
#6
REVIEW
Cornelis J F van Noorden, Bahar Yetkin-Arik, Paola Serrano Martinez, Noëlle Bakker, Mathilda E van Breest Smallenburg, Reinier O Schlingemann, Ingeborg Klaassen, Bernarda Majc, Anamarija Habic, Urban Bogataj, S Katrin Galun, Milos Vittori, Mateja Erdani Kreft, Metka Novak, Barbara Breznik, Vashendriya V V Hira
Lactate and ATP formation by aerobic glycolysis, the Warburg effect, is considered a hallmark of cancer. During angiogenesis in non-cancerous tissue, proliferating stalk endothelial cells (ECs) also produce lactate and ATP by aerobic glycolysis. In fact, all proliferating cells, both non-cancer and cancer cells, need lactate for the biosynthesis of building blocks for cell growth and tissue expansion. Moreover, both non-proliferating cancer stem cells in tumors and leader tip ECs during angiogenesis rely on glycolysis for pyruvate production, which is used for ATP synthesis in mitochondria through oxidative phosphorylation (OXPHOS)...
May 11, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38725415/correlation-of-trpa1-rnascope-and-agonist-responses
#7
JOURNAL ARTICLE
Natalia S Rojas-Galvan, Cosmin I Ciotu, Stefan Heber, Michael J M Fischer
The TRPA1 ion channel is a sensitive detector of reactive chemicals, found primarily on sensory neurons. The phenotype exhibited by mice lacking TRPA1 suggests its potential as a target for pharmacological intervention. Antibody-based detection for distribution analysis is a standard technique. In the case of TRPA1, however, there is no antibody with a plausible validation in knockout animals or functional studies, but many that have failed in this regard. To this end we employed the single molecule in situ hybridization technique RNAscope on sensory neurons immediately after detection of calcium responses to the TRPA1 agonist allyl isothiocyanate...
May 10, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38725414/increased-er-stress-and-unfolded-protein-response-activation-in-epithelial-and-inflammatory-cells-in-hypersensitivity-pneumonitis
#8
JOURNAL ARTICLE
Sandra Cabrera, Ángeles García-Vicente, Pamela Gutiérrez, Andrea Sánchez, Miguel Gaxiola, Carolina Rodríguez-Bobadilla, Moisés Selman, Annie Pardo
Several types of cytotoxic insults disrupt endoplasmic reticulum (ER) homeostasis, cause ER stress, and activate the unfolded protein response (UPR). The role of ER stress and UPR activation in hypersensitivity pneumonitis (HP) has not been described. HP is an immune-mediated interstitial lung disease that develops following repeated inhalation of various antigens in susceptible and sensitized individuals. The aim of this study was to investigate the lung expression and localization of the key effectors of the UPR, BiP/GRP78, CHOP, and sXBP1 in HP patients compared with control subjects...
May 10, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38725403/immunohistochemical-and-morphometric-assessment-on-the-biological-function-and-vascular-endothelial-cells-in-the-initial-process-of-cortical-porosity-in-mice-with-pth-administration
#9
JOURNAL ARTICLE
Miki Abe, Tomoka Hasegawa, Hiromi Hongo, Tomomaya Yamamoto, Yan Shi, Jiaxin Cui, Xuanyu Liu, Qi Yao, Hotaka Ishizu, Haruhi Maruoka, Hirona Yoshino, Mai Haraguchi-Kitakamae, Tomohiro Shimizu, Norio Amizuka
To clarify the cellular mechanism of cortical porosity induced by intermittent parathyroid hormone (PTH) administration, we examined the femoral cortical bone of mice that received 40 µg/kg/day (four times a day) human PTH (hPTH) (1-34). The PTH-driven cortical porosity initiated from the metaphyseal region and chronologically expanded toward the diaphysis. Alkaline phosphatase (ALP)-positive osteoblasts in the control mice covered the cortical surface, and endomucin-positive blood vessels were distant from these osteoblasts...
May 10, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38590114/transient-receptor-potential-channels-in-the-healthy-and-diseased-blood-brain-barrier
#10
REVIEW
Rita Rezzani, Gaia Favero, Marzia Gianò, Daniela Pinto, Mauro Labanca, Cornelis J F van Noorden, Fabio Rinaldi
The large family of transient receptor potential (TRP) channels are integral membrane proteins that function as environmental sensors and act as ion channels after activation by mechanical (touch), physical (heat, pain), and chemical stimuli (pungent compounds such as capsaicin). Most TRP channels are localized in the plasma membrane of cells but some of them are localized in membranes of organelles and function as intracellular Ca2+ -ion channels. TRP channels are involved in neurological disorders but their precise role(s) and relevance in these disorders are not clear...
April 8, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38553997/clearing-properties-between-coconut-oil-and-xylene-in-histological-tissue-processing
#11
JOURNAL ARTICLE
Owusu Afriyie Bright, Darko Nkansah Samuel, Musah Ayeley Adisa, Owusu Ohui Dorcas, Quartey Perez, Antwi Ama Melody, Addai Kusi Michael, Osei Sarpong Albert, Ebenezer Senu
Xylene is the commonest clearing agent even though it is hazardous and costly. This study evaluated the clearing properties of coconut oil as an alternative cost-effective clearing agent for histological processes. Ten (10) prostate samples fixed in formalin were taken and each one was cut into 4 before randomly separating them into four groups (A, B, C and D). Tissues were subjected to ascending grades of alcohol for dehydration. Group A was cleared in xylene and Groups B, C, and D were cleared at varying times of 1hr 30mins, 3hrs, and 4hrs in coconut oil respectively before embedding, sectioning, and staining were carried out...
March 30, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38544368/hmgb2-promotes-de-novo-lipogenesis-to-accelerate-hepatocyte-proliferation-during-liver-regeneration
#12
JOURNAL ARTICLE
Narantsog Choijookhuu, Koichi Yano, Baljinnyam Lkham-Erdene, Shinichiro Shirouzu, Toshiki Kubota, Fidya, Takumi Ishizuka, Kengo Kai, Etsuo Chosa, Yoshitaka Hishikawa
Liver regeneration is a well-orchestrated compensatory process that is regulated by multiple factors. We recently reported the importance of the chromatin protein, a high-mobility group box 2 (HMGB2) in mouse liver regeneration. However, the molecular mechanism remains unclear. In this study, we aimed to study how HMGB2 regulates hepatocyte proliferation during liver regeneration. Seventy-percent partial hepatectomy (PHx) was performed in wild-type (WT) and HMGB2-knockout (KO) mice, and the liver tissues were used for microarray, immunohistochemistry, quantitative polymerase chain reaction (qPCR), and Western blotting analyses...
March 27, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38454609/diabetes-as-a-pancreatic-microvascular-disease-a-pericytic-perspective
#13
REVIEW
Luciana Mateus Gonçalves, Catarina Andrade Barboza, Joana Almaça
Diabetes is not only an endocrine but also a vascular disease. Vascular defects are usually seen as consequence of diabetes. However, at the level of the pancreatic islet, vascular alterations have been described before symptom onset. Importantly, the cellular and molecular mechanisms underlying these early vascular defects have not been identified, neither how these could impact the function of islet endocrine cells. In this review, we will discuss the possibility that dysfunction of the mural cells of the microvasculature-known as pericytes-underlies vascular defects observed in islets in pre-symptomatic stages...
March 7, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38440794/relation-between-reactive-oxygen-species-production-and-transient-receptor-potential-vanilloid1-expression-in-human-skin-during-aging
#14
JOURNAL ARTICLE
Gaia Favero, Marzia Gianò, Caterina Franco, Daniela Pinto, Cornelis J F van Noorden, Fabio Rinaldi, Rita Rezzani
Skin sensitivity and impaired epidermal barrier function are associated with aging and are at least partly due to increased production of reactive oxygen species (ROS). Transient receptor potential vanilloid1 (TRPV1) is expressed in keratinocytes, fibroblasts, mast cells, and endothelial cells in skin. We investigated in skin biopsies of adult and elderly donors whether TRPV1 expression is involved in the skin aging process. We found that aging skin showed a strongly reduced epidermal thickness, strongly increased oxidative stress, protease expression, and mast cell degranulation and strongly increased TRPV1 expression both in epidermis and dermis...
March 5, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38439738/relevance-of-comet-assay-and-phosphorylated-hsp90%C3%AE-in-cancer-patients-peripheral-blood-leukocytes-as-tools-to-assess-cisplatin-based-chemotherapy-clinical-response-and-disease-outcome
#15
JOURNAL ARTICLE
Mayra L Sottile, Laura C Gómez, Analía Redondo, Jorge Ibarra, María B García, Lucía Gonzalez, Laura M Vargas-Roig, Silvina B Nadin
Cisplatin (cPt) is a commonly used treatment for solid tumors. The main target of its cytotoxicity is the DNA molecule, which makes the DNA damage response (DDR) crucial for cPt-based chemotherapy. Therefore, it is essential to identify biomarkers that can accurately predict the individual clinical response and prognosis. Our goal was to assess the usefulness of alkaline comet assay and immunocytochemical staining of phosphorylated Hsp90α (p-Hsp90α), γH2AX, and 53BP1 as predictive/prognostic markers...
March 5, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38506280/special-topics-series-special-issue-on-endothelial-cell-and-pericyte-homeostasis-and-pathogenesis
#16
EDITORIAL
Stephen M Hewitt
No abstract text is available yet for this article.
March 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38400717/imaging-of-isolated-exosomes-by-correlative-microscopy
#17
JOURNAL ARTICLE
Şeyda Demir, Esra Erdal, Hüsnü Alper Bagriyanik
Correlative microscopy is a sophisticated imaging technique that combines optical and electron microscopes, with the most common approach being the integration of light microscopy and electron microscopy, known as correlative light and electron microscopy (CLEM). While CLEM provides a comprehensive view of biological samples, it presents a significant challenge in sample preparation due to the distinct processes involved in each technique. Striking a balance between these methods is crucial. Despite numerous approaches, achieving seamless imaging with CLEM remains a complex task...
February 24, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38189179/identification-of-terminal-%C3%AE-glcnac-on-brachyspira-species-in-human-intestinal-spirochetosis
#18
JOURNAL ARTICLE
Hisanori Matoba, Mai Iwaya, Chifumi Fujii, Jun Nakayama
Human intestinal spirochetosis (HIS) is a colorectal bacterial infection caused by the Brachyspira species. Griffonia simplicifolia -II (GS-II) is a lectin specific to terminal α/βGlcNAc residues. Here, we investigated terminal βGlcNAc residues in the context of HIS infection using GS-II-horseradish peroxidase staining and HIK1083 immunostaining specific to terminal αGlcNAc residues. Fourteen of 15 HIS cases were GS-II-positive on the bacterial body. No cases showed HIK1083 positivity...
February 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38288702/acellular-extrinsic-fiber-cementum-is-invariably-present-in-the-superficial-layer-of-apical-cementum-in-mouse-molar
#19
JOURNAL ARTICLE
Hajime Iwama, Masaru Kaku, Lay Thant, Masaru Mizukoshi, Moe Arai, Yoshiki Ono, Kohei Kitami, Isao Saito, Katsumi Uoshima
The cementum is a highly mineralized tissue that covers the tooth root. The regional differences among the types of cementum, especially in the extrinsic fibers that contribute to tooth support, remain controversial. Therefore, this study used second harmonic generation imaging in conjunction with automated collagen extraction and image analysis algorithms to facilitate the quantitative examination of the fiber characteristics and the changes occurring in these fibers over time. Acellular extrinsic fiber cementum (AEFC) was invariably observed in the superficial layer of the apical cementum in mouse molars, indicating that this region of the cementum plays a crucial role in supporting the tooth...
January 30, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://read.qxmd.com/read/38264898/evaluation-of-extracellular-matrix-remodeling-in-full-thickness-skin-grafts-in-mice
#20
JOURNAL ARTICLE
Anton Erik Tjust, Urban Hellman, Antonios Giannopoulos, Annika Winsnes, Karin Strigård, Ulf Gunnarsson
Abdominal hernia is a protruding weakness in the abdominal wall. It affects abdominal strength and life quality and can lead to complications due to intestinal entrapment. Autologous full-thickness skin graft (FTSG) has recently become an alternative material for reinforcement in the surgical repair of large abdominal hernias instead of synthetic mesh. FTSG eventually integrates with the abdominal wall, but the long-term fate of the graft itself is not fully understood. This has implications as to how these grafts should be optimally used and handled intraoperatively...
January 24, 2024: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
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