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International Journal of Molecular Medicine

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https://read.qxmd.com/read/30720050/nucleolin-promotes-ang-ii%C3%A2-induced-phenotypic-transformation-of-vascular-smooth-muscle-cells-via-interaction-with-tropoelastin-mrna
#1
Li Fang, Peng-Fei Zhang, Kang-Kai Wang, Zhi-Lin Xiao, Mei Yang, Zai-Xin Yu
The current study aimed to clarify the role of nucleolin in the phenotypic transformation of vascular smooth muscle cells (VSMCs) and to preliminarily explore its underlying mechanism. The spatial and temporal expression patterns of nucleolin, and the effects of angiotensin II (Ang II) on the expression of VSMC phenotypic transformation markers, α‑smooth muscle‑actin, calponin, smooth muscle protein 22α and osteopontin were investigated. The effects of nucleolin on VSMC phenotypic transformation and the expression of phenotypic transformation‑associated genes, tropoelastin, epiregulin and fibroblast growth factor 2 (b‑FGF), were determined...
February 4, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720058/salidroside-protects-pc-12-cells-against-amyloid-%C3%AE-induced-apoptosis-by-activation-of-the-erk1-2-and-akt-signaling-pathways
#2
Zheng-Luan Liao, Heng Su, Yun-Fei Tan, Ya-Ju Qiu, Jun-Peng Zhu, Yan Chen, Si-Si Lin, Ming-Hao Wu, Yan-Ping Mao, Jiao-Jiao Hu, En-Yan Yu
Alzheimer's disease (AD) is one of the most frequent diseases in elderly people and causes high mortality. Its incidence is increasing annually and no effective therapeutic treatment currently exists. In the present study, salidroside, a major active ingredient of Rhodiola rosea, was able to protect PC‑12 cells from the toxicity and apoptosis induced by AD inducer amyloid (A)β1‑42. Salidroside significantly protected PC‑12 cells by inhibiting Aβ1‑42‑induced cytotoxicity and mitochondria‑mediated endogenous caspase apoptotic pathways...
February 1, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720049/beclin1-inhibition-enhances-paclitaxel%C3%A2-mediated-cytotoxicity-in-breast-cancer-in-vitro-and-in-vivo
#3
Cheng-Lin Wu, Jian-Fei Liu, Yan Liu, Yu-Xiao Wang, Kai-Fei Fu, Xiao-Jie Yu, Qian Pu, Xiu-Xiu Chen, Li-Jun Zhou
Beclin1, a key regulator of autophagy, has been demonstrated to be associated with cancer cell resistance to chemotherapy. Paclitaxel is a conventional chemotherapeutic drug used in the clinical treatment of breast cancer. However, the function and mechanism of Beclin1 in paclitaxel‑mediated cytotoxicity in breast cancer are not well defined. The present study demonstrated that paclitaxel suppressed cell viability and Beclin1 expression levels in BT‑474 breast cancer cells in a dose‑ and time‑dependent fashion...
February 1, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720061/bioinformatic-analysis-of-next%C3%A2-generation-sequencing-data-to-identify-dysregulated-genes-in-fibroblasts-of-idiopathic-pulmonary-fibrosis
#4
Chau-Chyun Sheu, Wei-An Chang, Ming-Ju Tsai, Ssu-Hui Liao, Inn-Wen Chong, Po-Lin Kuo
Idiopathic pulmonary fibrosis (IPF) is a lethal fibrotic lung disease with an increasing global burden. It is hypothesized that fibroblasts have a number of functions that may affect the development and progression of IPF. However, the present understanding of cellular and molecular mechanisms associated with fibroblasts in IPF remains limited. The present study aimed to identify the dysregulated genes in IPF fibroblasts, elucidate their functions and explore potential microRNA (miRNA)‑mRNA interactions. mRNA and miRNA expression profiles were obtained from IPF fibroblasts and normal lung fibroblasts using a next‑generation sequencing platform, and bioinformatic analyses were performed in a step‑wise manner...
January 31, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720060/nr2f2-loss%C3%A2-of%C3%A2-function-mutation-is-responsible-for-congenital-bicuspid-aortic-valve
#5
Juan Wang, Pradhan Abhinav, Ying-Jia Xu, Ruo-Gu Li, Min Zhang, Xing-Biao Qiu, Ruo-Min Di, Qi Qiao, Xiu-Mei Li, Ri-Tai Huang, Song Xue, Yi-Qing Yang
Congenital bicuspid aortic valve (BAV) represents the most common type of cardiac birth defect affecting 0.4‑2% of the general population, and accounts for a markedly increased incidence of life‑threatening complications, including valvulopathy and aortopathy. Accumulating evidence has demonstrated the genetic basis of BAV. However, the genetic basis for BAV in the majority of cases remains to be elucidated. In the present study, the coding regions and splicing donors/acceptors of the nuclear receptor subfamily 2 group F member 2 (NR2F2) gene, which encodes a transcription factor essential for proper cardiovascular development, were sequenced in 176 unrelated cases of congenital BAV...
January 31, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720055/inhibition-of-acid%C3%A2-sensing-ion-channel-1a-attenuates-acid%C3%A2-induced-activation-of-autophagy-via-a-calcium-signaling-pathway-in-articular-chondrocytes
#6
Wen-Fan Gao, Ya-Yun Xu, Jin-Fang Ge, Fei-Hu Chen
Acid‑sensing ion channel 1a (ASIC1a), member of the degenerin/epithelial sodium channel protein superfamily, serves a critical role in various physiological and pathological processes. The aim of the present study was to examine the role of ASIC1a in the autophagy of rat articular chondrocytes. Autophagy was induced by acidic stimulation in rat articular chondrocytes and the extent of autophagy was evaluated via the expression levels of microtubule‑associated protein 1 light chain 3II, Beclin1 and uncoordinated‑51 like kinase1...
January 31, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720053/metformin-prevents-nephrolithiasis-formation-by-inhibiting-the-expression-of-opn-and-mcp-1-in-vitro-and-in-vivo
#7
Xiong Yang, Tong Yang, Jie Li, Rui Yang, Shiyong Qi, Yang Zhao, Liang Li, Jingjin Li, Xuening Zhang, Kuo Yang, Yong Xu, Chunyu Liu
Treatment targeting osteopontin (OPN) and monocyte chemoattractant protein 1 (MCP‑1) has been recognized as a novel approach in renal crystal formation. The present study was designed to investigate the suppressive effects of metformin on nephrolithiasis formation and its potential mechanism. The cytotoxicity of metformin on MDCK and HK‑2 cells was determined using a Cell Counting Kit‑8 assay in vitro. Subsequently, the mRNA transcription and protein expression levels of MCP‑1 and OPN were detected by reverse transcription‑quantitative‑polymerase chain reaction analysis, western blot analysis and ELISA...
January 30, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720059/microrna%C3%A2-199a%C3%A2-5p-functions-as-a-tumor-suppressor-in-oral-squamous-cell-carcinoma-via-targeting-the-ikk%C3%AE-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway
#8
Dongyi Wei, Baohong Shen, Weixin Wang, Yanjun Zhou, Xiaodong Yang, Guangjian Lu, Jianbin Yang, Yuebao Shao
MicroRNAs (miRNAs) have been shown to have a significant role in the progression of several types of cancer, including oral squamous cell carcinoma (OSCC). However, the biological function and regulatory mechanisms of miRNAs in OSCC remain to be fully elucidated. The aim of the present study was to investigate the role of miRNAs in OSCC and the relevant mechanism. Using a microarray, it was found that miRNA (miR)‑199a‑5p was one of the most downregulated miRNAs in OSCC tissues. A low expression of miR‑199a‑5p was closely associated with tumor differentiation, lymph node metastasis, tumor‑node‑metastasis stage, and overall survival rate...
January 29, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720057/mir-183-modulated-cell-proliferation-and-apoptosis-in-ovarian-cancer-through-the-tgf-%C3%AE-smad4-signaling-pathway
#9
Junhui Zhou, Caixia Zhang, Bo Zhou, Daqiong Jiang
An increasing body of evidence has revealed that the aberrant expression of microRNAs (miRNAs/miRs) is involved in the development and progression of ovarian cancer (OC). miR‑183 has been demonstrated to act as a tumor suppressor and oncogene in various types of human cancers. However, the biological role of miR‑183 in OC still remains unclear. The aim of the present study was to investigate the role of miR‑183 and evaluate its underlying mechanism in OC. In the present study, miR‑183 was observed to be upregulated in OC tissues and cell lines as determined by reverse transcription‑quantitative polymerase chain reaction...
January 29, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720046/mir-135-regulated-breast-cancer-proliferation-and-epithelial-mesenchymal-transition-acts-by-the-wnt-%C3%AE-catenin-signaling-pathway
#10
Daqiong Jiang, Bo Zhou, Yan Xiong, Hongbing Cai
Breast cancer (BC) is the most common cancer in women around the world. microRNAs (miRNAs/miRs) have been proved to be associated with the development and progression of breast cancer. In the present study, to elucidate the effects of dysregulated miR‑135 on cells and underlying mechanisms in BC, in vitro and in vivo experiments were conducted. The biological functions of miR‑135 were studied using MTT, colony formation, wound healing, transwell assays as well as tumorigenicity analysis. Gain‑ and loss‑ of function of miR‑135 studies revealed that ectopic expression of miR‑135 in MDA‑MB‑468 and MCF‑7 cells significantly inhibited cell growth, migration, invasion and EMT, at least in part through inhibiting the activation of the Wnt/β‑catenin pathway...
January 29, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720051/nrdp1-is-involved-in-hippocampus-apoptosis-in-cardiopulmonary-bypass-induced-cognitive-dysfunction-via-the-regulation-of-erbb3-protein-levels
#11
Jie Zheng, Su Min, Bin Hu, Qin Liu, Yunqiang Wan
The cardiopulmonary bypass (CPB) is an important risk factor for the development of postoperative cognitive dysfunction (POCD). The pathological mechanism of the neuro‑modulation receptor degradation protein ring finger protein 41 (Nrdp1) in CPB‑induced cognitive dysfunction remains unclear. In the present study, aged Sprague‑Dawley male rats and CPB treatment were selected to duplicate the POCD model. A hypoxia/reoxygeneration (H/R) model was established to evaluate the effect of Nrdp1 in vitro. Apoptosis in the hippocampus regions were measured using a terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling assay, the viability and apoptosis level of the cells were measured via an MTT assay and flow cytometry, respectively, and the expression levels of Nrdp1, erb‑b2 receptor tyrosine kinase 3 (ErbB3), phosphorylated‑protein kinase B (p‑AKT) and cleaved (c‑) caspase‑3 were detected using western blot analysis...
January 28, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720047/-corrigendum-methanol-extract-of-guettarda-speciosa-linn-inhibits-the-production-of-inflammatory-mediators-through-the-inactivation-of-syk-and-jnk-in-macrophages
#12
Hien Thi Thu Le, Young-Chang Cho, Sayeon Cho
After the acceptance of the article and during the pre‑publication stages, the author listed second on the paper, Young‑Chang Cho, moved to a new affiliation (College of Pharmacy, Chonnam National University, Gwangju 61186, Korea). Therefore, the published paper should also have included details of the new affiliation as a 'Present address'. The author and affiliation information should have been shown as follows: HIEN THI THU LE1*, YOUNG‑CHANG CHO1,2* and SAYEON CHO1. 1Laboratory of Molecular Pharmacological Cell Biology, College of Pharmacy, Chung‑Ang University, Seoul 06974, Republic of Korea...
January 28, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30720064/anti%C3%A2-inflammatory-effects-of-6%C3%A2-formyl-umbelliferone-via-the-nf%C3%A2-%C3%AE%C2%BAb-and-erk-mapk-pathway-on-lps%C3%A2-stimulated-raw-264-7-cells
#13
Sang-Bo Kim, Min-Jae Kang, Chang-Won Kang, Nan-Hee Kim, Hyung Wook Choi, Hyun Ah Jung, Jae Sue Choi, Gun-Do Kim
Inhibition of over‑activated inflammation has been demonstrated as one of the most efficient strategies for treating inflammatory diseases. In the present study, 6‑formyl umbelliferone (6FU) was used to evaluate its anti‑inflammatory effects on lipopolysaccharide (LPS)‑stimulated RAW 264.7 macrophages. 6FU inhibited chronic inflammatory processes, including increasing nitric oxide levels, and the expression of pro‑inflammatory genes and producing cytokines was investigated by a nitrite assay and reverse transcription‑polymerase chain reaction, respectively...
January 25, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30747215/microrna-101-inhibits-autophagy-to-alleviate-liver-ischemia-reperfusion-injury-via-regulating-the-mtor-signaling-pathway
#14
Hu Song, Chenyang Du, Xingxing Wang, Jianjun Zhang, Zhongyang Shen
Liver ischemia/reperfusion injury (LIRI) is a common complication of liver surgery, and affects liver function post‑transplantation. However, the precise mechanism underlying LIRI has not yet been completely elucidated. Previous studies have demonstrated the involvement of a number of microRNAs (miRNAs/miRs) in liver pathophysiology. The objective of the present study was to determine the potential function and mechanism of miR‑101‑mediated regulation of autophagy in LIRI. Compared with the sham‑treated group, a significant decrease in miR‑101 and mechanistic target of rapamycin (mTOR) expression levels following ischemia/reperfusion (IR) were observed, along with an increased number of autophagosomes (P<0...
January 24, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30747229/microrna%C3%A2-223-attenuates-lps%C3%A2-induced-inflammation-in-an-acute-lung-injury-model-via-the-nlrp3-inflammasome-and-tlr4-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway-via-rhob
#15
Yurong Yan, Kexin Lu, Ting Ye, Zongwang Zhang
Acute lung injury (ALI) and the more severe acute respiratory distress syndrome are common and complex inflammatory lung diseases. MicroRNAs (miRs) have emerged as novel gene regulatory molecules, serving a crucial role in a variety of complex diseases, including ALI. In the present study, the anti‑inflammatory action of miR‑223 on inflammation in ALI was demonstrated and the possible mechanism was further examined. In lipopolysaccharide‑induced ALI, the expression of miR‑223 was reduced compared with that in the control normal group...
January 23, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30747228/rapamycin%C3%A2-induced-mir%C3%A2-30a-downregulation-inhibits-senescence-of-vsmcs-by-targeting-beclin1
#16
Pan Tan, Haiqin Wang, Junkun Zhan, Xinyu Ma, Xingjun Cui, Yanjiao Wang, Yi Wang, Jiayu Zhong, Youshuo Liu
Vascular senescence is considered to be an independent risk factor for cardiovascular diseases. The present study aimed to investigate the effects of rapamycin on miR‑30a and its relationship with autophagy and senescence in vascular smooth muscle cells (VSMCs). Young and aging VSMCs were treated with rapamycin or transfected with miR‑30a mimics. Measurement of cellular senescence was conducted using senescence‑associated (SA)‑β‑Galactosidase (gal) staining. Dual luciferase reporter assay was used to confirm binding for miR‑30a and Beclin1...
January 23, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30747216/protective-effect-of-a-hydrogen-sulfide-donor-on-balloon-injury-induced-restenosis-via-the-nrf2-hif-1%C3%AE-signaling-pathway
#17
Ken Ling, Ancong Xu, Yunfei Chen, Xueyin Chen, Yiqing Li, Weici Wang
Restenosis is liable to occur following treatment with endovascular interventional therapy. Increasing evidence indicates that hydrogen sulfide (H2S) exhibits numerous physiological properties, including antioxidative and cardioprotective disease properties. Thus, the present study aimed to investigate the anti‑restenosis effects of H2S and its protective mechanisms. A balloon dilatation restenosis model was used, in which model Sprague‑Dawley rats were treated with sodium hydrosulfide (NaHS: A donor of H2S, 30 µmol/kg) by intraperitoneal injection for 4 weeks...
January 23, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30747210/mir%C3%A2-335-promotes-stress-granule-formation-to-inhibit-apoptosis-by-targeting-rock2-in-acute-ischemic-stroke
#18
Wenwen Si, Shanyu Ye, Zhenxing Ren, Xin Liu, Zimei Wu, Yi Li, Jianhong Zhou, Saixia Zhang, Yiwei Li, Rudong Deng, Dongfeng Chen
Under harmful environmental conditions, stress granules (SGs), macromolecular aggregates that are associated with cell survival and death, are produced in the eukaryotic cytoplasm. However, whether and how microRNAs (miRNAs/miRs) modulate SG formation induced by acute ischemic stroke has not been investigated. In the present study, a rat model of middle cerebral artery occlusion (MCAO) was utilized and miRNA array profiling and reverse transcription‑quantitative polymerase chain reaction were performed. The results revealed that miR‑335 was downregulated during acute ischemic stroke, which was concomitant with reduced SG formation, enhanced apoptosis levels and increased Rho associated protein kinase 2 (ROCK2) expression...
January 22, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30664197/fibroblast-growth-factor-21-alleviates-hypoxia-reoxygenation-injury-in-h9c2-cardiomyocytes-by-promoting-autophagic-flux
#19
Zhong Ren, Weijin Xiao, Yun Zeng, Mi-Hua Liu, Guo-Hua Li, Zhi-Han Tang, Shun-Lin Qu, Ya-Meng Hao, Hou-Qin Yuan, Zhi-Sheng Jiang
Fibroblast growth factor (FGF)‑21, a member of the family of FGFs, exhibits protective effects against myocardial ischemia and ischemia/reperfusion injury; it is also an enhancer of autophagy. However, the mechanisms underlying the protective role of FGF‑21 against cardiomyocyte hypoxia/reoxygenation (H/R) injury remain unclear. The present study aimed to investigate the effect of FGF‑21 on H9c2 cardiomyocyte injury induced by H/R and the mechanism associated with changes in autophagy. Cultured H9c2 cardiomyocytes subjected to hypoxia were treated with a vehicle or FGF‑21 during reoxygenation...
January 21, 2019: International Journal of Molecular Medicine
https://read.qxmd.com/read/30664190/adiponectin-protects-against-uric-acid%C3%A2-induced-renal-tubular-epithelial-inflammatory-responses-via-the-adipor1-ampk-signaling-pathway
#20
Qingmei Yang, Chensheng Fu, Xiaoli Zhang, Zhenxing Zhang, Jianan Zou, Jing Xiao, Zhibin Ye
Adiponectin (APN) exerts anti‑inflammatory effects in various cells. Uric acid (UA) induces inflammation in proximal renal tubular epithelial cells (PTECs). It remains unknown whether APN protects against UA‑induced inflammation. In the present study, human PTECs were incubated with 100 µg/ml soluble (S) UA in the presence or absence of globular (g) APN, APN receptor 1 (AdipoR1)‑short hairpin RNA lentivirus or compound C. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assays were performed to assess APN mRNA expression...
January 21, 2019: International Journal of Molecular Medicine
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