Jochen Weile, Gabrielle Ferra, Gabriel Boyle, Sriram Pendyala, Clara Amorosi, Chiann-Ling Yeh, Atina G Cote, Nishka Kishore, Daniel Tabet, Warren van Loggerenberg, Ashyad Rayhan, Douglas M Fowler, Maitreya J Dunham, Frederick P Roth
MOTIVATION: Long read sequencing technologies, an attractive solution for many applications, often suffer from higher error rates. Alignment of multiple reads can improve base-calling accuracy, but some applications, e.g. sequencing mutagenized libraries where multiple distinct clones differ by one or few variants, require the use of barcodes or unique molecular identifiers. Unfortunately, sequencing errors can interfere with correct barcode identification, and a given barcode sequence may be linked to multiple independent clones within a given library...
April 3, 2024: Bioinformatics