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Journal of Oral Science

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https://read.qxmd.com/read/30760661/prolonged-release-of-iloprost-enhances-pulpal-blood-flow-and-dentin-bridge-formation-in-a-rat-model-of-mechanical-tooth-pulp-exposure
#1
Chalida N Limjeerajarus, Seang Sonntana, Limothai Pajaree, Chansawang Kansurang, Supaphol Pitt, Thumsing Saowapa, Pavasant Prasit
The aim of this study was to investigate the effect of prolonged release of iloprost, a prostacyclin analog, on angiogenesis and dental pulp healing in a rat model of mechanical pulp exposure. The profile of iloprost release from poly (lactic-co-glycolic) acid (PLGA) microspheres was evaluated, and expression of vascular endothelial growth factor (VEGF) mRNA was determined. The molars of rats were subjected to mechanical pulp exposure and 5 different forms of treatment: Ca(OH)2 , PLGA (blank), iloprost, and iloprost/PLGA...
February 13, 2019: Journal of Oral Science
https://read.qxmd.com/read/30726799/in-vivo-antimicrobial-activity-of-silver-diammine-fluoride-on-carious-lesions-in-dentin
#2
Maribasappa Karched, Dena Ali, Hien Ngo
Minimally invasive treatment protocols may leave a residual layer of carious dentin, which requires treatment for the inhibition of bacterial growth beneath restorations. We aimed to examine the in vivo effect of silver diammine fluoride (SDF) and SDF + potassium iodide (KI) application on bacteria present in deep carious lesions. We studied the in vivo efficacy in five patients, each of which had five carious lesions. Dentin samples taken before and after treatment were subjected to microbial analyses. Following treatment with SDF, the median colony-forming unit (CFU) counts per mg of dentin reduced from 9 × 105 to 1...
February 6, 2019: Journal of Oral Science
https://read.qxmd.com/read/30713266/deionized-water-can-substitute-common-bleaching-agents-for-nonvital-tooth-bleaching-when-treated-with-non-thermal-atmospheric-plasma
#3
Burak Çelik, İsmail D Çapar, Fatma İbiş, Necdet Erdilek, Utku K Ercan
The bleaching efficacy of common bleaching agents and deionized water treated with non-thermal atmospheric pressure plasma in the pulp chamber for nonvital tooth bleaching was evaluated. A total of 120 extracted human maxillary first incisors were stained using human blood. Teeth were randomly divided into eight groups (n = 15). In the first four groups, teeth were bleached using 35% hydrogen peroxide gel, 37% carbamide peroxide gel, 2:1 (w/v) sodium perborate paste, and deionized water for 30 min. In the remaining groups, bleaching agents were treated with non-thermal atmospheric plasma for 5 min inside the pulp chamber...
February 1, 2019: Journal of Oral Science
https://read.qxmd.com/read/30713265/shaping-effectiveness-and-surface-topography-of-reciprocating-files-after-multiple-simulated-uses
#4
Valentina Spicciarelli, Giacomo Corsentino, Hani F Ounsi, Marco Ferrari, Simone Grandini
This in vitro study assessed morphological changes and efficiency of reciprocating files after multiple uses. Sixty standardized Endo Training Blocks and 10 ReciprocR25 files were selected (six blocks for each file). Each file was its own control (before use vs. after each instrumentation). The instruments were used according to the manufacturer's instructions, and scanning electron microscopy was used to observe fatigue cracks, metal strips/metal flash, pitting, fretting, debris, disruption of the cutting edge, and plastic deformations after each instrumentation...
February 1, 2019: Journal of Oral Science
https://read.qxmd.com/read/30713264/molecular-biological-and-histological-effects-of-er-yag-laser-irradiation-on-tooth-movement
#5
Yuji Tsuka, Ryo Kunimatsu, Hidemi Gunji, Kengo Nakajima, Tomoka Hiraki, Ayaka Nakatani, Kotaro Tanimoto
The aim of this study was to evaluate the effects of a fiberotomy-like procedure using Er:YAG laser irradiation on the velocity of orthodontic tooth movement. To produce experimental tooth movement in rats, orthodontic force was applied to the upper first molars with a nickel-titanium closed coil. The right molars were irradiated with an Er:YAG laser while the non-irradiated left molars were used as controls. The rats were sacrificed at 4 weeks after the start of tooth movement and the distance between the mesial side of the second molar and the distal side of the upper first molar was measured on CT images...
February 1, 2019: Journal of Oral Science
https://read.qxmd.com/read/30713263/effect-of-mechanical-strain-induced-pge-2-production-on-bone-nodule-formation-by-rat-calvarial-progenitor-cells
#6
Yasuyo Takahashi, Kotoe Mayahara, Rena Fushiki, Rieko Matsuike, Noriyoshi Shimizu
High-magnitude mechanical strain inhibits bone nodule formation by reducing expression of bone morphogenetic protein-2 (BMP-2), Runt-related transcription factor 2 (Runx2), and muscle segment homeobox 2 (Msx2). Mechanical strain also induces production of proinflammatory factor prostaglandin E2 (PGE2 ) by osteoblasts. We measured the effect of mechanical strain-induced PGE2 production on bone nodule formation and expression levels of bone formation-related factors. Osteoblast-like cells isolated from fetal rat calvariae were loaded with 18% cyclic tension force (TF) for 48 h in the presence or absence of NS-398, a selective inhibitor of cyclooxygenase-2...
February 1, 2019: Journal of Oral Science
https://read.qxmd.com/read/30568047/long-term-clinical-and-radiographic-evaluation-of-the-effectiveness-of-direct-pulp-capping-materials
#7
Takashi Matsuura, Viviane K S Kawata-Matsuura, Shizuka Yamada
The aim of this review was to assess the effectiveness of different direct pulp-capping (DPC) materials for human pulp-exposed teeth. An electronic search was performed on 20 February 2018. Long-term clinical and radiographic evaluations of the effectiveness of different DPC materials for use on human pulp-exposed teeth were included. Risk-of-bias assessment and data extraction were performed. From the 496 identified articles, 15 met the eligibility criteria. Among the studies included in those articles, a total of 1,322 teeth were treated with 12 types of DPC materials, and 1,136 teeth were evaluated at a final follow-up examination...
December 20, 2018: Journal of Oral Science
https://read.qxmd.com/read/30568046/change-in-muscle-hardness-after-trigger-point-injection-and-physiotherapy-for-myofascial-pain-syndrome
#8
Akiko Okada-Ogawa, Naohiko Sekine, Kosuke Watanabe, Ryutaro Kohashi, Sayaka Asano, Koich Iwata, Yoshiki Imamura
Assessment and treatment of masticatory myofascial pain syndrome (MPS) are not standardized and remain controversial. We examined whether muscle hardness was useful for evaluating masticatory MPS and analyzed the effectiveness of treatments such as stretching and massage (SM) and trigger point injection (TPI). Twenty healthy volunteers and 20 MPS patients were enrolled. MPS patients were divided into TPI and SM treatment groups. Hardness of masticatory muscle with a taut band (TB) and change in hardness were evaluated after SM and TPI treatments...
December 20, 2018: Journal of Oral Science
https://read.qxmd.com/read/30541990/the-effect-of-mesenchymal-stem-cells-on-osteoclast-precursor-cell-differentiation
#9
Takaharu Abe, Keisuke Sumi, Ryo Kunimatsu, Nanae Oki, Yuji Tsuka, Kengo Nakajima, Kazuyo Ando, Kotaro Tanimoto
Transplantation of mesenchymal stem cells (MSCs) has been extensively studied in the field of regenerative medicine. Bone regeneration is achieved via the interaction of osteoblasts and osteoclasts. However, the influence of MSCs on osteoclasts is unknown. The purpose of this study was to investigate the effect of MSCs on the expression of genes for osteoclast differentiation factors using qPCR after indirect co-culture of MSCs and RAW264 cells. The numbers of osteoclasts after addition of soluble receptor activator of nuclear factor kappa B (NF-κB) ligand (sRANKL) were also compared...
December 11, 2018: Journal of Oral Science
https://read.qxmd.com/read/29848890/cell-migration-capability-of-vascular-endothelial-growth-factor-into-the-root-apex-of-a-root-canal-model-in-vivo
#10
Takashi Matsuura, Kouji Sugimoto, Viviane K S Kawata-Matsuura, Kajiro Yanagiguchi, Shizuka Yamada, Yoshihiko Hayashi
Once a tooth develops deep caries and the dental pulp tissue is irreversibly infected, the infected dental pulp tissue should be removed, and filling material should be placed in the root canal. Endodontically treated teeth are prone to root fracture or periapical periodontitis; however, dental pulp tissue has the potential to prevent root fracture or periapical periodontitis. Therefore, dental pulp regeneration after pulpectomy may help prolong tooth life. In this study, a new method of dental pulp regeneration was developed...
December 29, 2018: Journal of Oral Science
https://read.qxmd.com/read/29984786/periodontal-status-of-buccally-and-palatally-impacted-maxillary-canines-after-surgical-orthodontic-treatment-with-open-technique
#11
Stefano Mummolo, Alessandro Nota, Maria Elena De Felice, Domenico Marcattili, Simona Tecco, Giuseppe Marzo
This study investigated differences in periodontal health variables between buccally impacted maxillary canines (BIMC) and palatally impacted maxillary canines (PIMC) after surgical-orthodontic treatment with open technique. Nineteen patients were enrolled: 10 with unilateral BIMC (5 men, 5 women; mean age 18.50 ± 1.96 years) and 9 with unilateral PIMC (4 men, 5 women; mean age 19.44 ± 2.40 years). Probing depth and keratinized tissue were recorded 12 months after surgical-orthodontic treatment, and the differences between the 2 sides were analyzed as primary outcomes...
December 27, 2018: Journal of Oral Science
https://read.qxmd.com/read/30429438/effects-of-xylitol-containing-chewing-gum-on-the-oral-microbiota
#12
Kenji Takeuchi, Mikari Asakawa, Takafumi Hashiba, Toru Takeshita, Youji Saeki, Yoshihisa Yamashita
In this interventional study, a randomized controlled trial was used to evaluate the short-term effects of xylitol-containing chewing gum on the salivary microbiota. In total, 70 healthy adult men recruited from the Japan Ground Self Defense Force participated in the study during a 2-day training at Yamaguchi camp, Yamaguchi Prefecture, Japan. The men were randomly divided into two groups: one group chewed two pieces of xylitol-containing chewing gum 7 times/day for 2 days (n = 34) and the other did not (n = 36)...
November 15, 2018: Journal of Oral Science
https://read.qxmd.com/read/30429437/expression-of-the-forkhead-box-transcription-factor-foxo3a-in-human-periapical-granulomas
#13
Kae Ishii, Keisuke Hatori, Osamu Takeichi, Kosuke Makino, Kazuma Himi, Hiroki Komiya, Bunnai Ogiso
It has been reported that Forkhead box transcription factor class O3a (Foxo3a) is expressed in rheumatoid arthritis, a chronic inflammatory condition accompanied by bone resorption, and plays a role in its pathology. However, it has remained unclear whether Foxo3a is involved in the pathogenesis of periapical granulomas. The present study was performed to compare the expression of Foxo3a in periapical granulomas and healthy gingival tissues. Samples were obtained surgically from patients, and subjected to hematoxylin-eosin staining for histopathologic diagnosis...
November 15, 2018: Journal of Oral Science
https://read.qxmd.com/read/30429436/differential-dendritic-cell-responses-to-cell-wall-mannan-of-candida-albicans-candida-parapsilosis-and-candida-dubliniensis
#14
Thu N Y Nguyen, Oranart Matangkasombut, Patcharee Ritprajak
Mannan (mannosylated glycoproteins) in the outermost layer of the Candida cell wall may be the first molecules that interact with host dendritic cells (DCs) and activate immune responses that determine disease outcomes. However, little is known about how different mannan structures of common oral Candida species affect DC activation. The effects of heat-inactivated (HI) yeast cells and soluble mannan of Candida albicans, Candida parapsilosis, and Candida dubliniensis on bone marrow-derived DC (BMDC) responses were compared...
November 15, 2018: Journal of Oral Science
https://read.qxmd.com/read/30429435/retrospective-clinical-study-of-296-patients-with-mass-lesions-of-the-tongue
#15
Yusuke Miyake, Keiji Shinozuka, Kosuke Ueki, Jun Teraoka, Manabu Zama, Shouhei Ogisawa, Yasuhisa Shinozaki, Junya Aoki, Keiichi Yanagawa, Osamu Shimizu, Tadayoshi Kaneko, Morio Tonogi, Hidero Ohki
To better understand the clinical features of mass lesions of the tongue, we retrospectively evaluated frequency, recurrence rate, and complications in 296 patients who had undergone surgery for such lesions. The diagnoses were fibroma (43.6%), mucous cyst (14.2%), papilloma (11.8%), hemangioma (7.8%), granuloma (6.4%), lipoma (1.4%), schwannoma (1.0%), ectopic tonsil (0.7%), and other (13.2%). Recurrence was noted in two patients (0.7%). Twenty-two patients (7.4%) developed surgical complications, including lingual nerve paralysis (6...
November 15, 2018: Journal of Oral Science
https://read.qxmd.com/read/30369560/trends-in-domiciliary-dental-care-including-the-need-for-oral-appliances-and-dental-technicians-in-japan
#16
Shingo Kamijo, Kumiko Sugimoto, Meiko Oki, Yumi Tsuchida, Tetsuya Suzuki
The need for domiciliary dental care (DDC) for people requiring long-term nursing care is increasing as the super-aged society of Japan grows still older. Dysphagia diagnosis and rehabilitation are becoming more important in DDC; thus, the need for prostheses used for dysphasia rehabilitation is presumed to be increasing. To identify DDC trends in Japan, as well as the need for prostheses and dental technicians for DDC, we sent a self-administered questionnaire to dentists providing DDC and analyzed responses from 138 dentists (valid response rate, 39...
October 26, 2018: Journal of Oral Science
https://read.qxmd.com/read/30369559/comparison-of-the-bone-augmentation-ability-of-absorbable-collagen-sponge-with-that-of-hydroxyapatite-collagen-composite
#17
Yasumasa Ozawa, Tatsuya Kubota, Takanobu Yamamoto, Naoya Tsukune, Ryosuke Koshi, Tetsuya Nishida, Masatake Asano, Shuichi Sato
The present study was designed to compare the bone augmentation ability of absorbable collagen sponge (ACS) with that of hydroxyapatite/collagen composite (HAP/Col) using a rat calvaria defect model. Bone defects were created artificially on the surface of the calvariae of 10-week-old male Fisher rats, and then cylindral plastic caps filled with ACS or HAP/Col were placed on the defects. This area was designated as the region of interest (ROI) and new bone formation was observed at 0, 4, 8, and 12 weeks after surgery using micro-CT...
October 26, 2018: Journal of Oral Science
https://read.qxmd.com/read/30369558/risk-factors-for-failure-of-orthodontic-mini-screws-placed-in-the-median-palate
#18
Michiko Ichinohe, Mitsuru Motoyoshi, Mizuki Inaba, Yasuki Uchida, Mari Kaneko, Reiko Matsuike, Noriyoshi Shimizu
This study investigated the stability of mini-screws placed in the median palate. The study included 25 patients (7 males, 18 females; mean age, 23.4 ± 5.6 years; age range, 15.0-34.5 years) who had mini-screws placed during orthodontic treatment at Nihon University School of Dentistry Dental Hospital. Mini-screws (diameter, 2.0 mm; length, 9.0 mm) were placed in the median palatal region; the first screw was inserted mesiodistally at the distal contact of the maxillary first molar, and the second screw was placed 6-9 mm mesial to the first screw...
October 26, 2018: Journal of Oral Science
https://read.qxmd.com/read/30158339/il-1%C3%AE-and-tnf-%C3%AE-regulate-mouse-amelotin-gene-transcription-in-gingival-epithelial-cells
#19
Keisuke Noda, Mizuho Yamazaki, Yasunobu Iwai, Sari Matsui, Ayako Kato, Hideki Takai, Yohei Nakayama, Yorimasa Ogata
Amelotin (AMTN) is an enamel protein expressed in maturation-stage ameloblasts and junctional epithelium. To clarify the transcriptional regulation of the AMTN gene by interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), we conducted real-time PCR, Western blotting, transient transfection analyses with luciferase constructs including various lengths of the mouse AMTN gene promoter, and gel shift and chromatin immunoprecipitation assays using mouse gingival epithelial GE1 cells. The levels of AMTN mRNA and protein in GE1 cells were increased after 6 h of stimulation with IL-1β (1 ng/mL) and TNF-α (10 ng/mL)...
September 23, 2018: Journal of Oral Science
https://read.qxmd.com/read/30146537/effect-of-a-silane-and-phosphate-functional-monomer-on-shear-bond-strength-of-a-resin-based-luting-agent-to-lithium-disilicate-ceramic-and-quartz-materials
#20
Serina Taguchi, Futoshi Komine, Kei Kubochi, Ryosuke Fushiki, Fumiaki Kimura, Hideo Matsumura
This study examined the effect of silane and phosphate functional monomer on bond strengths between a resin-based luting agent joined to a lithium disilicate ceramic (IPS e.max) and silica (quartz) materials. The e.max and quartz specimens were assigned to 6 groups with different priming/bonding agents, namely, Clearfil Porcelain Bond Activator, Clearfil Photo Bond, Clearfil Photo Bond Universal with Clearfil Porcelain Bond Activator, Clearfil Photo Bond Catalyst with Clearfil Porcelain Bond Activator, Clearfil Photo Bond with Clearfil Porcelain Bond Activator, and unprimed...
September 23, 2018: Journal of Oral Science
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