Blanche C Ip, Samantha J Madnick, Sophia Zheng, Tessa C A van Tongeren, Susan J Hall, Hui Li, Suzanne Martin, Sandrine Spriggs, Paul Carmichael, Wei Chen, David Ames, Lori A Breitweiser, Heather E Pence, Andrew J Bowling, Kamin J Johnson, Richard Cubberley, Jeffrey R Morgan, Kim Boekelheide
Chemicals in the systemic circulation can undergo hepatic xenobiotic metabolism, generate metabolites and exhibit altered toxicity compared to their parent compounds. This paper describes a two-chamber liver-organ co-culture model in a higher-throughput 96-well format for the determination of toxicity on target tissues in the presence of physiologically relevant human liver metabolism. This two-chamber system is a hydrogel formed within each well consisting of a central well (target tissue) and an outer ring-shaped trough (human liver tissue)...
February 9, 2024: Toxicological Sciences: An Official Journal of the Society of Toxicology