Takeyuki Goto, Tomoki Sasaki, Yong Chong, Masahiro Taniguchi, Jae Man Lee, Akitsu Masuda, Takeru Ebihara, Kenichiro Shiraishi, Naoki Tani, Akiko Yonekawa, Kei Gondo, Hiroyuki Kuwano, Nobuyuki Shimono, Hideyuki Ikematsu, Koichi Akashi, Takahiro Kusakabe
BACKGROUND: A cost-effective and eco-friendly method is needed for the assessment of humoral immunity against SARS-CoV-2 in large populations. OBJECTIVE: We investigated the performance of an ELISA that uses silkworm-produced proteins to quantify the strain-specific anti-Spike IgG (anti-S IgG) titer. METHODS: The OD values for the anti-His-tag antibody, a standard material of ELISA quantification, were measured. Correlations between the ELISA for each strain and the Abbott SARS-CoV-2 IgG II Quant assay for the wild type were evaluated with serum samples from nine participants with various infection and vaccination statuses...
July 1, 2023: Human Antibodies