journal
Journals Extremophiles : Life Under Ext...

Extremophiles : Life Under Extreme Conditions

https://read.qxmd.com/read/37906310/halorubrum-hochsteinianum-sp-nov-an-ancient-haloarchaeon-from-a-natural-experiment
#21
JOURNAL ARTICLE
Russell H Vreeland, Ya-Ping Sun, Bei-Bei Wang, Jing Hou, Heng-Lin Cui
A single extremely halophilic strain was isolated from salt brine produced when a fresh water lake flooded a large salt mine located beneath the lake. The water that entered this mine contained less than 0.34 M NaCl, but over time, this sealed brine became saturated by Cenozoic age salt (121-125 million-year BCE). The isolated strain requires at least 1.7 M NaCl for survival and grows optimally in 3.1 M NaCl. Therefore, it could not have survived or been present in the waters that flooded this salt mine...
October 31, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37848738/disorder-and-amino-acid-composition-in-proteins-their-potential-role-in-the-adaptation-of-extracellular-pilins-to-the-acidic-media-where-acidithiobacillus-thiooxidans-grows
#22
JOURNAL ARTICLE
Edgar D Páez-Pérez, Araceli Hernández-Sánchez, Elvia Alfaro-Saldaña, J Viridiana García-Meza
There are few biophysical studies or structural characterizations of the type IV pilin system of extremophile bacteria, such as the acidophilic Acidithiobacillus thiooxidans. We set out to analyze their pili-comprising proteins, pilins, because these extracellular proteins are in constant interaction with protons of the acidic medium in which At. thiooxidans grows. We used the web server Operon Mapper to analyze and identify the cluster codified by the minor pilin of At. thiooxidans. In addition, we carried an in-silico characterization of such pilins using the VL-XT algorithm of PONDR® server...
October 17, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37847335/production-of-poly-3-hydroxybutyrate-and-extracellular-polymeric-substances-from-glycerol-by-the-acidophile-acidiphilium-cryptum
#23
JOURNAL ARTICLE
Ernesto González, Camila Zuleta, Guiselle Zamora, Nataly Maturana, Belén Ponce, María Virginia Rivero, Alberto Rodríguez, Juan Pablo Soto, Felipe Scott, Álvaro Díaz-Barrera
Acidiphilium cryptum is an acidophilic, heterotrophic, and metallotolerant bacteria able to use dissolved oxygen or Fe(III) as an electron sink. The ability of this extremophile to accumulate poly(3-hydroxybutyrate) (PHB) and secrete extracellular polymeric substances (EPS) has also been reported. Hence, the aim of this work is to characterize the production of PHB and EPS by the wild strain DSM2389 using glycerol in shaken flasks and bioreactor. Results showed that maximum PHB accumulation (37-42% w/w) was obtained using glycerol concentrations of 9 and 15 g L-1 , where maximum dry cell weight titers reached 3...
October 17, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37847305/properties-of-the-extracellular-alkaline-inulinase-produced-by-haloalkaliphilic-phototrophic-bacteria-ectothiorodospirea-mobilis
#24
JOURNAL ARTICLE
Levon Markosyan, Arevik Vardanyan
The studies have revealed alkaline exoinulinase produced by haloalkaliphilic phototrophic bacteria Ectothiorhodospirea mobilis Al-2 for the first time. A new method for the isolation of a homogeneous exoinulinase from the culture broth was developed and the properties of this enzyme have been investigated. It was shown that specified exoinulinase in contrast to the studied exoinulinases produced by microorganisms exhibits catalytic activity at the wide range of pH (7.0-10) and a temperature (20-60 °C) with a maximum of the inulolitic activity at pH 9...
October 17, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37843723/pyrofollis-japonicus-gen-nov-sp-nov-a-novel-member-of-the-family-pyrodictiaceae-isolated-from-the-iheya-north-hydrothermal-field
#25
JOURNAL ARTICLE
Urara Miyazaki, Masaru Sanari, Akihiro Tame, Masaaki Kitajima, Akihiro Okamoto, Shigeki Sawayama, Junichi Miyazaki, Ken Takai, Satoshi Nakagawa
A novel hyperthermophilic, heterotrophic archaeon, strain YC29T , was isolated from a deep-sea hydrothermal vent in the Mid-Okinawa Trough, Japan. Cells of strain YC29T were non-motile, irregular cocci with diameters of 1.2-3.0 µm. The strain was an obligatory fermentative anaerobe capable of growth on complex proteinaceous substrates. Growth was observed between 85 and 100 °C (optimum 90-95 °C), pH 4.9-6.4 (optimum 5.1), and in the presence of 1.4-4.0% (w/v) NaCl (optimum 3.0%)...
October 16, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37839067/extremophilic-microbial-metabolism-and-radioactive-waste-disposal
#26
REVIEW
Sarah Jane Butterworth, Franky Barton, Jonathan Richard Lloyd
Decades of nuclear activities have left a legacy of hazardous radioactive waste, which must be isolated from the biosphere for over 100,000 years. The preferred option for safe waste disposal is a deep subsurface geological disposal facility (GDF). Due to the very long geological timescales required, and the complexity of materials to be disposed of (including a wide range of nutrients and electron donors/acceptors) microbial activity will likely play a pivotal role in the safe operation of these mega-facilities...
October 15, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37712998/structure-function-studies-of-the-nad-dependent-dna-ligase-from-the-poly-extremophile-deinococcus-radiodurans-reveal-importance-of-the-brct-domain-for-dna-binding
#27
JOURNAL ARTICLE
Andreia Fernandes, Adele Williamson, Pedro M Matias, Elin Moe
Bacterial NAD+ -dependent DNA ligases (LigAs) are enzymes involved in replication, recombination, and DNA-repair processes by catalyzing the formation of phosphodiester bonds in the backbone of DNA. These multidomain proteins exhibit four modular domains, that are highly conserved across species, with the BRCT (breast cancer type 1 C-terminus) domain on the C-terminus of the enzyme. In this study, we expressed and purified both recombinant full-length and a C-terminally truncated LigA from Deinococcus radiodurans (DrLigA and DrLigA∆BRCT) and characterized them using biochemical and X-ray crystallography techniques...
September 15, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37709928/genetic-engineering-of-low-temperature-polyhydroxyalkanoate-production-by-acidovorax-sp-a1169-a-psychrophile-isolated-from-a-subglacial-outflow
#28
JOURNAL ARTICLE
Jakub Grzesiak, Jan Gawor, Małgorzata Marta Rogala, Xenie Kouřilová, Stanislav Obruča
In recent years, extremophilic microorganisms have been employed as producers of the microbial bioplastics polyhydroxyalkanoates (PHA), which are of great biotechnological value. Nevertheless, cold-loving or psychrophilic (cryophilic) bacteria have been neglected in this regard. Here, we present an investigation of the Arctic glacier-derived PHA producer Acidovorax sp. A1169. Biolog GEN III Microplates were used as a screening tool to identify the most suitable carbon substrate concerning PHA synthesis. The strain produced homopolymer poly(3-hydroxybutyrate) (PHB) most efficiently (2 g/L) at a temperature of 15 °C when supplied with fructose or mannitol as carbon sources with a substantial decrease of PHB biosynthesis at 17...
September 14, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37668803/vertical-segregation-and-phylogenetic-characterization-of-archaea-and-archaeal-ammonia-monooxygenase-gene-in-the-water-column-of-the-western-arctic-ocean
#29
JOURNAL ARTICLE
Puthiya Veettil Vipindas, Thajudeen Jabir, Siddarthan Venkatachalam, Eun Jin Yang, Anand Jain, Kottekkatu Padinchati Krishnan
Archaea constitute a substantial fraction of marine microbial biomass and play critical roles in the biogeochemistry of oceans. However, studies on their distribution and ecology in the Arctic Ocean are relatively scarce. Here, we studied the distributions of archaea and archaeal ammonia monooxygenase (amoA) gene in the western Arctic Ocean, using the amplicon sequencing approach from the sea surface to deep waters up to 3040 m depth. A total of five archaeal phyla, Nitrososphaerota, "Euryarchaeota", "Halobacteriota," "Nanoarchaeota", and Candidatus Thermoplasmatota, were detected...
September 5, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37646861/correction-quantitative-evaluation-of%C3%A2-endogenous-reference-genes-for-ddpcr-under-salt-stress-using-a-moderate-halophile
#30
Qinghua Xing, Noha M Mesbah, Haisheng Wang, Jun Li, Baisuo Zhao
No abstract text is available yet for this article.
August 30, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37584877/the-genus-anoxybacillus-an-emerging-and-versatile-source-of-valuable-biotechnological-products
#31
REVIEW
Deisiane Fernanda da Rosa, Alexandre José Macedo
Thermophilic and alkaliphilic microorganisms are unique organisms that possess remarkable survival strategies, enabling them to thrive on a diverse range of substrates. Anoxybacillus, a genus of thermophilic and alkaliphilic bacteria, encompasses 24 species and 2 subspecies. In recent years, extensive research has unveiled the diverse array of thermostable enzymes within this relatively new genus, holding significant potential for industrial and environmental applications. The biomass of Anoxybacillus has demonstrated promising results in bioremediation techniques, while the recently discovered metabolites have exhibited potential in medicinal experiments...
August 16, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37548679/functional-expression-of-an-antimicrobial-peptide-belonging-to-halocin-c8-family-from-natrinema-sp-rns21-in-escherichia-coli
#32
JOURNAL ARTICLE
Chang Ho Ri, Song Ryong Li, Chon Il Paek, Yu Sin Kim
Halocins, the proteinaceous antimicrobial agents produced by haloarchaea, may be used for the preservation of salted foods and the treatment of diseases. For their application and function explanation, it is necessary to produce the active recombinants. In this work, a haloarchaeal strain producing halocin was isolated from the salt-fermented shrimp and identified as Natrinema sp. RNS21 by 16S rRNA gene sequence analysis. From 1 L of RNS21 culture, about 0.32 mg of halocin with 96% purity was obtained...
August 7, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37481762/molecular-evolution-steered-structural-adaptations-in-the-dna-polymerase-iii-%C3%AE-subunit-of-halophilic-bacterium-salinibacter-ruber
#33
JOURNAL ARTICLE
Aveepsa Sengupta, Kunwali Das, Nidhi Jha, Yusuf Akhter, Ashutosh Kumar
A significant portion of the earth has a salty environment, and the literature on bacterial survival mechanisms in salty environments is limited. During molecular evolution, halophiles increase acidic amino acid residues on their protein surfaces which leads to a negatively charged surface potential that helps them to maintain the protein integrity and protect them from denaturation by competing with salt ions. Through protein family analysis, we have investigated the molecular-level adaptive features of DNA polymerase III's catalytic subunit (alpha) and its structure-function relationship...
July 23, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37481751/genomic-basis-for-the-unique-phenotype-of-the-alkaliphilic-purple-nonsulfur-bacterium-rhodobaca-bogoriensis
#34
JOURNAL ARTICLE
Michael T Madigan, Kelly S Bender, Sophia A Sanguedolce, Mary N Parenteau, Marisa H Mayer, Yukihiro Kimura, Zheng-Yu Wang-Otomo, W Matthew Sattley
Although several species of purple sulfur bacteria inhabit soda lakes, Rhodobaca bogoriensis is the first purple nonsulfur bacterium cultured from such highly alkaline environments. Rhodobaca bogoriensis strain LBB1T was isolated from Lake Bogoria, a soda lake in the African Rift Valley. The phenotype of Rhodobaca bogoriensis is unique among purple bacteria; the organism is alkaliphilic but not halophilic, produces carotenoids absent from other purple nonsulfur bacteria, and is unable to grow autotrophically or fix molecular nitrogen...
July 23, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37428266/recombinant-expression-and-characterization-of-a-new-laccase-bioinformatically-identified-from-the-antarctic-thermophilic-bacterium-geobacillus-sp-id17
#35
JOURNAL ARTICLE
Rodrigo Cortés-Antiquera, Sebastián L Márquez, Giannina Espina, Jorge Sánchez-SanMartín, Jenny M Blamey
Geobacillus sp. ID17 is a gram-positive thermophilic bacterium isolated from Deception Island, Antarctica, which has shown to exhibit remarkable laccase activity in crude extract at high temperatures. A bioinformatic search using local databases led to the identification of three putative multicopper oxidase sequences in the genome of this microorganism. Sequence analysis revealed that one of those sequences contains the four-essential copper-binding sites present in other well characterized laccases. The gene encoding this sequence was cloned and overexpressed in Escherichia coli, partially purified and preliminary biochemically characterized...
July 10, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37418077/survival-of-icelandic-airborne-microbes-towards-simulated-atmospheric-stress-factors
#36
JOURNAL ARTICLE
Aurélien Daussin, Pauline Vannier, Émilien Mater, Tina Šantl-Temkiv, Charles Cockell, Viggó Þór Marteinsson
Surface microbes are aerosolized into the atmosphere by wind and events such as dust storms, wildland fires, and volcano eruptions. Only microbial cells that survive the various atmospheric stressors during their transportation will deposit and colonize new environments. These stressors include desiccation, oxidative stress, solar radiation, osmotic shock, and freeze-thaw cycles. In this paper, we specifically studied the survival of representative microbial model strains isolated from the atmosphere over pristine volcanic landscapes to understand their potential to successfully disperse to novel terrestrial environments...
July 7, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37410158/selection-of-antarctic-yeasts-as-gray-mold-biocontrol-agents-in-strawberry
#37
JOURNAL ARTICLE
Eskálath Morganna Silva Ferreira, Gabriela Garmendia, Vívian Nicolau Gonçalves, Juliana Fonseca Moreira da Silva, Luiz Henrique Rosa, Silvana Vero, Raphael Sanzio Pimenta
The postharvest disease popularly known as gray mold is considered one of the most limiting factors strawberry fruit production. The most effective way to control this disease is still the use of chemical fungicides. However, other alternative sources of control are being explored. Among these, psychrophilic yeasts adapted to extreme conditions, such as those found in the Antarctic region, may have great potential for use as biocontrol agents. Thus, the present study aimed to select psychrotolerant yeasts obtained from Antarctic region and to evaluate their potential for biocontrol under gray mold, caused by Botrytis cinerea in strawberries stored at low temperature...
July 6, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37400737/salinilacihabitans-rarus-gen-nov-sp-nov-natrononativus-amylolyticus-gen-nov-sp-nov-natronobeatus-ordinarius-gen-nov-sp-nov-and-halovivax-gelatinilyticus-sp-nov-halophilic-archaea-isolated-from-a-salt-lake-and-soda-lakes
#38
JOURNAL ARTICLE
Xin-Xin Li, Shun Tan, Mu Cheng, Yao Hu, Xue Ma, Jing Hou, Heng-Lin Cui
Four halophilic archaea strains, AD-4T , CGA30T , CGA73T , and WLHSJ27T , were isolated from a salt lake and two soda lakes located in different regions of China. The 16S rRNA and rpoB' gene sequence similarities among strains AD-4T , CGA30T , CGA73T , WLHSJ27T , and the current species of the family Natrialbaceae were 90.9-97.5% and 83.1-91.8%, respectively. The phylogenetic and phylogenomic analyses revealed that these four strains separated from existing genera in the family Natrialbaceae and formed distant branches...
July 3, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37354217/identification-and-genome-wide-analysis-provide-insights-into-the-genetic-diversity-and-biotechnological-potentials-of-novel-cold-adapted-acinetobacter-strain
#39
JOURNAL ARTICLE
Hajra Kayani, Muhammad Asif Rasheed, Wadi B Alonazi, Farrukh Jamil, Annam Hussain, ChangHui Yan, Raza Ahmed, Muhammad Ibrahim
Extreme cold environments, such as polar regions or high-altitude mountains, are known for their challenging conditions including low temperatures, high salinity, and limited nutrient availability. Microbes that thrive in these environments have evolved specialized strategies to survive and function under such harsh conditions. The study aims to identify, sequence the genome, perform genome assembly, and conduct a comparative genome-wide analysis of Acinetobacter sp. strain P1, which was isolated from the Batura glacier regions of Pakistan...
June 24, 2023: Extremophiles: Life Under Extreme Conditions
https://read.qxmd.com/read/37349574/polyhydroxyalkanoate-biosynthesis-and-optimisation-of-thermophilic-geobacillus-stearothermophilus-strain-k4e3_spr_npp
#40
JOURNAL ARTICLE
Seema Prabhudev Rodge, Kundalik Shivaji Shende, Niranjan Prakashrao Patil
Polyhydroxyalkanoates (PHA) can be used to combat the challenges associated with plastic because it is biodegradable and can be produced from renewable resources. Extremophiles are considered to be potential PHA producers. An initial screening for the PHA synthesizing ability of a thermophilic bacteria Geobacillus stearothermophilus strain K4E3_SPR_NPP was carried out using Sudan black B staining. Nile red viable colony staining was used to further verify that the isolates produced PHA. Crotonic acid assays were used to determine the concentrations of PHA...
June 22, 2023: Extremophiles: Life Under Extreme Conditions
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