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Current Protocols in Neuroscience

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https://read.qxmd.com/read/30901512/recombinant-viral-vectors-as-neuroscience-tools
#1
Shih-Heng Chen, Juhee Haam, Mitzie Walker, Erica Scappini, John Naughton, Negin P Martin
Recombinant viruses are highly efficient vehicles for in vivo gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans-synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno-associated viruses, retro/lentiviruses, and glycoprotein-deleted rabies viruses...
March 22, 2019: Current Protocols in Neuroscience
https://read.qxmd.com/read/30883041/production-of-viral-constructs-for-neuroanatomy-calcium-imaging-and-optogenetics
#2
Shih-Heng Chen, Juhee Haam, Mitzie Walker, Erica Scappini, John Naughton, Negin P Martin
Advances in design and use of light-sensitive and light-emitting sensors have facilitated observation, measurement, and control of neuronal activities. Viruses are effective vectors for delivery of these valuable research tools to mammalian brains. Recombinant viruses are optimized to mediate regulatable, long-term, and cell-specific gene expression. Here, we describe production methods for three of the most commonly used types of recombinant viruses in neurobiology research: adeno-associated virus (AAV), retrovirus/lentivirus, and glycoprotein-deleted rabies virus...
March 18, 2019: Current Protocols in Neuroscience
https://read.qxmd.com/read/30861320/in-vivo-electroporation-and-time-lapse-imaging-of-the-rostral-migratory-stream-in-developing-rodent-brain
#3
Zhihui Huang, Ying Wang
Interneurons in the olfactory bulb are generated from neuronal precursor cells migrating from the anterior subventricular zone (SVZa) throughout the embryonic and postnatal life of mammals. This article describes basic methods for in vivo electroporation to label SVZa cells of both embryonic and postnatal rats. In addition, it describes three methods for tracing SVZa progenitors and following their migration pathway and differentiation, including immunohistochemistry, time-lapse live imaging in slice culture, and time-lapse imaging following transplantation in slice culture...
March 12, 2019: Current Protocols in Neuroscience
https://read.qxmd.com/read/30791216/detection-and-quantification-of-multiple-rna-sequences-using-emerging-ultrasensitive-fluorescent-in-situ-hybridization-techniques
#4
Larissa Erben, Andres Buonanno
Fluorescent detection of transcripts using RNAscope has quickly become a standard in situ hybridization (ISH) approach in neuroscience with over 400 publications since its introduction in 2012. RNAscope's sensitivity and specificity allow the simultaneously detection of up to three low abundance mRNAs in single cells (i.e., multiplexing) and, in contrast to other ISH techniques, RNAscope is performed in 1 day. BaseScope, a newer ultrasensitive platform, uses improved amplification chemistry of single oligonucleotide probe pairs (∼50 bases)...
February 21, 2019: Current Protocols in Neuroscience
https://read.qxmd.com/read/30791212/analysis-of-neuro-neuronal-synapses-using-embryonic-chick-ciliary-ganglion-via-single-axon-tracing-electrophysiology-and-optogenetic-techniques
#5
Ryo Egawa, Hiromu Yawo
The calyx-type synapse is a giant synaptic structure in which a presynaptic terminal wraps around a postsynaptic neuron in a one-to-one manner. It has been used for decades as an experimental model system of the synapse due to its simplicity and high accessibility in physiological recording methods. In particular, the calyx of the embryonic chick ciliary ganglion (CG) has enormous potential for synapse science because more flexible genetic manipulations are available compared with other synapses. Here, we describe methods to study presynaptic morphology, physiology, and development using CGs and cutting-edge molecular tools...
February 21, 2019: Current Protocols in Neuroscience
https://read.qxmd.com/read/30387915/high-throughput-analysis-of-behavior-under-the-control-of-optogenetics-in-caenorhabditis-elegans
#6
Alex J Yu, Troy A McDiarmid, Evan L Ardiel, Catharine H Rankin
In this unit, we describe an inexpensive and versatile method for optogenetic stimulation of a large population of genetically engineered Caenorhabditis elegans worms while quantitatively analyzing behavior. A custom light-emitting diode light source is used to deliver blue-light stimuli, causing direct depolarization of neurons expressing the light-gated cation channel Channelrhodopsin-2, which in turn evokes behavioral responses. The behavioral responses are recorded by a high-throughput machine vision-based tracking system, the Multi-Worm Tracker, for detailed analysis...
November 2, 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30315730/miniscope-grin-lens-system-for-calcium-imaging-of-neuronal-activity-from-deep-brain-structures-in-behaving-animals
#7
Lifeng Zhang, Bo Liang, Giovanni Barbera, Sarah Hawes, Yan Zhang, Kyle Stump, Ira Baum, Yupeng Yang, Yun Li, Da-Ting Lin
Visualizing neural activity from deep brain regions in freely behaving animals through miniature fluorescent microscope (miniscope) systems is becoming more important for understanding neural encoding mechanisms underlying cognitive functions. Here we present our custom-designed miniscope GRadient INdex (GRIN) lens system that enables simultaneously recording from hundreds of neurons for months. This article includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis...
October 13, 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30285322/neocortical-microdissection-at-columnar-and-laminar-resolution-for-molecular-interrogation
#8
Koen Kole, Tansu Celikel
The heterogeneous organization of the mammalian neocortex poses a challenge for elucidating the molecular mechanisms underlying its physiological processes. Although high-throughput molecular methods are increasingly deployed in neuroscience, their anatomical specificity is often lacking. In this unit, we introduce a targeted microdissection technique that enables extraction of high-quality RNA and proteins at high anatomical resolution from acutely prepared brain slices. We exemplify its utility by isolating single cortical columns and laminae from the mouse primary somatosensory (barrel) cortex...
October 4, 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30248240/measuring-pain-avoidance-like-behavior-in-drug-dependent-rats
#9
Amanda R Pahng, Scott Edwards
In contrast to their analgesic properties, excessive use of either opioids or alcohol produces a paradoxical emergence of heightened pain sensitivity to noxious stimuli, termed hyperalgesia, which may promote increased use of opioids or alcohol drinking to manage worsening pain symptoms. Hyperalgesia has traditionally been measured in rodents via reflex-based assays, including the von Frey method. To better model the motivational and affective dimensions of pain in a state of opioid/alcohol dependence and withdrawal, this unit describes the use of a non-reflex-based method for measuring pain avoidance-like behavior in dependent rats...
October 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30265442/mouse-models-of-pain-in-sickle-cell-disease
#10
Varun Sagi, Waogwende L Song-Naba, Barbara A Benson, Sonal S Joshi, Kalpna Gupta
Sickle cell disease (SCD) is a genetic blood disorder that impacts millions of individuals worldwide. SCD is characterized by debilitating pain that can begin during infancy and may continue to increase throughout life. This pain can be both acute and chronic. A characteristic feature specific to acute pain in SCD occurs during vaso-occlusive crisis (VOC) due to the blockade of capillaries with sickle red blood cells. The acute pain of VOC is intense, unpredictable, and requires hospitalization. Chronic pain occurs in a significant population with SCD...
September 28, 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30204300/dual-channel-photostimulation-for-independent-excitation-of-two-populations
#11
Bryan M Hooks
Manipulation of defined neurons using excitatory opsins, including channelrhodopsin, enables studies of connectivity and the functional role of these circuit components in the brain. These techniques are vital in the neocortex, where diverse neurons are intermingled, and stimulation of specific cell types is difficult without the spatial, temporal, and genetic control available with optogenetic approaches. Channelrhodopsins are effective for mapping excitatory connectivity from one input type to its target...
September 11, 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/29944213/assessing-individual-neuronal-activity-across-the-intact-brain-using-hybridization-chain-reaction-hcr-to-detect-arc-mrna-localized-to-the-nucleus-in-volumes-of-cleared-brain-tissue
#12
Emily E Kramer, Patrick E Steadman, Jonathan R Epp, Paul W Frankland, Sheena A Josselyn
Arc (activity-regulated cytoskeleton-associated protein) is an immediate early gene that may be used to label recently active neurons. Arc is transcribed following neuronal activity, and its mRNA is then rapidly transported to dendrites. This feature allows nuclear-localized Arc mRNA to define ensembles of recently active neurons in systems or circuit neuroscience. However, typical in situ hybridization techniques severely constrain the thickness of the tissue specimen (typically 20-µm brain slices). Here, we describe a protocol for visualizing intranuclear Arc mRNA in large (4 × 4 × 3 mm) volumes of intact mouse brain tissue...
July 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/29944206/a-compact-head-mounted-endoscope-for-in-vivo-calcium-imaging-in-freely-behaving-mice
#13
Alexander D Jacob, Adam I Ramsaran, Andrew J Mocle, Lina M Tran, Chen Yan, Paul W Frankland, Sheena A Josselyn
Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool for investigating the relationship between behavior and population-level neuronal activity in rodents. However, commercially available miniature microscopes may be costly and, because they are closed source, may not be easily modified based on particular experimental requirements. Here, we describe how to build and use a low-cost compact head-mounted endoscope (CHEndoscope) system for in vivo calcium imaging. The CHEndoscope uses an implanted gradient index lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice...
July 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/29927097/behavioral-modulation-by-social-experiences-in-rodent-models
#14
Alexei Morozov
The lasting behavioral changes elicited by social signals provide important adaptations for survival of organisms that thrive as a group. Unlike the rapid innate responses to social cues, such adaptations have been understudied. Here, the rodent models of the lasting socially induced behavioral changes are presented as either modulations or reinforcements of the distinct forms of learning and memory or non-associative changes of affective state. The purpose of this categorization is to draw attention to the potential mechanistic links between the neuronal pathways that process social cues and the neuronal systems that mediate the well-studied forms of learning and memory...
July 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040229/functional-pituitary-tissue-generation-from-human-embryonic-stem-cells
#15
Mayuko Kano, Hidetaka Suga, Takatoshi Kasai, Chikafumi Ozone, Hiroshi Arima
The anterior pituitary gland produces several hormones essential for regulation of the somatic endocrine system. Deficiency of these hormones can cause life-threatening diseases, including adrenal crisis. Pituitary tissue generated from human pluripotent stem cells is expected to provide better treatment than current hormone replacement therapy. During early mammalian development, the pituitary anlage (Rathke's pouch) develops from non-neural ectoderm adjacent to the developing ventral hypothalamus. The close interaction between these two tissues is crucial for Rathke's pouch development and involves several signaling molecules...
April 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040221/overview-of-huntington-s-disease-models-neuropathological-molecular-and-behavioral-differences
#16
Claudia Rangel-Barajas, George V Rebec
Transgenic mouse models of Huntington's disease (HD), a neurodegenerative condition caused by a single gene mutation, have been transformative in their ability to reveal the molecular processes and pathophysiological mechanisms underlying the HD behavioral phenotype. Three model categories have been generated depending on the genetic context in which the mutation is expressed: truncated, full-length, and knock-in. No single model, however, broadly replicates the behavioral symptoms and massive neuronal loss that occur in human patients...
April 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040215/clarity-and-immunofluorescence-on-mouse-brain-tissue
#17
Tingting Yu, Dan Zhu
Tissue optical clearing techniques have provided essential tools for visualization of neural networks in unsectioned brain tissue. Here, we describe a passive optical clearing method based on hydrogel embedding, PACT (passive clarity technique), which is relatively simple. Advantages of PACT include tissue transparency, fluorescence preservation, and immunostaining compatibility for obtaining three-dimensional structures of mouse brain tissue. Additionally, it can enhance the penetration of antibodies in immunostaining and allows efficient immunolabeling of large volumes...
April 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040208/a-brief-guide-to-studying-fear-in-developing-rodents-important-considerations-and-common-pitfalls
#18
Caitlin S M Cowan, Rick Richardson
Development is a time of rapid change that sets the pathway to adult functioning across all aspects of physical and mental health. Developmental studies can therefore offer insight into the unique needs of individuals at different stages of normal development as well as the etiology of various disease states. The aim of this overview is to provide an introduction to the practical implementation of developmental studies in rats and mice, with an emphasis on the study of learned fear. We first discuss how developmental factors may influence experimental outcomes for any study...
April 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040206/fear-conditioning-by-proxy-social-transmission-of-fear-between-interacting-conspecifics
#19
Carolyn E Jones, Laura Agee, Marie-H Monfils
We describe a method of social fear transmission to a discrete auditory cue in freely behaving rats. Extending beyond traditional observer/demonstrator paradigms, rats are allowed to physically interact and integrate cues from all sensory modalities. In the protocol described in this article, "observer" rats experience social fear conditioning through a proxy cage mate that serves as a "demonstrator" during retrieval of a cued fear memory. We find that a specific auditory cue can come to elicit fear expression in an animal with no foot shock experience simply by interacting with a conspecific expressing a conditioned response in the presence of an otherwise benign stimulus...
April 2018: Current Protocols in Neuroscience
https://read.qxmd.com/read/30040200/resting-state-fmri-in-rodents
#20
Wen-Ju Pan, Jacob Billings, Maysam Nezafati, Anzar Abbas, Shella Keilholz
Resting state functional MRI (fMRI) and functional connectivity are widely applied in humans to examine the role of brain networks in normal function and dysfunction. A similar approach can be taken in rodents, either to obtain translational measures in models of brain disorders or to more carefully examine the neurophysiological underpinnings of the networks. A protocol for resting state functional connectivity in the anesthetized rat, from animal setup to data acquisition to possible pipelines for data analysis, is described...
April 2018: Current Protocols in Neuroscience
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