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Clinical Laboratory

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https://read.qxmd.com/read/30775903/determination-of-free-psa-fpsa-and-fpsa-psa-ratio-using-a-point-of-care-device
#1
Inês Anselmo da Costa, Jörg Hennenlotter, Tilman Todenhöfer, Eva Neumann, Susanne Deininger, Stefan Aufderklamm, Jens Bedke, Arnulf Stenzl, Steffen Rausch
BACKGROUND: Prostate specific antigen (PSA) and free PSA (fPSA) are important tools for diagnosing prostate cancer (PC). Efforts are continuously undertaken to provide more patient-centered healthcare. The application of point-of-care (POC) systems for laboratory analyses represents a step in this direction. Previous investigations on total PSA measurements using a POC system (concile® Ω100 POC reader) showed good concordance with standard laboratory measurements. For the same POC reader a novel system for fPSA was developed...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775902/investigation-of-fibronectin-binding-protein-fbp-and-panton-valentine-leukocidin-pvl-viulance-factors-in-clinical-methicillin-sensitive-and-resistant-staphylococcus-aureus-strains
#2
Neslihan Cihanoglu, Riza Adaleti, Yasar Nakipoglu
BACKGROUND: We aimed to investigate the frequency of fibronectin binding protein (FBP), which is part of the first step of adhesion, and Panton-Valentine leukocidin (PVL) toxin, which contributes to the destruction of host leukocytes and tissue necrosis, in clinical S. aureus strains. METHODS: One hundred S. aureus strains were included in the study and distributed as follows; 33 from skinwound swabs and catheter tips (SWCT), 33 from body fluid and secretion specimens (BSFS) such as tracheal aspirate, sputum, and pleural effusion fluid, 18 from tissue biopsy specimens (TBS), 10 specimens from blood, and related specimens (BRS) such as bone marrow, and cerebral spinal fluid, and six specimens from mucosal membrane of pharynx, nose, and vagina (MMS)...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775901/the-effect-of-different-blood-drawing-methods-on-hemolysis-and-test-results-from-intravenous-catheters-used-in-emergency-departments
#3
Cemal Kazezoglu, Erdinc Serin
BACKGROUND: Hemolysis is frequently reported in samples sent from emergency departments. In our study we aimed to compare the influence of invitro hemolysis on test results and hemolysis ratios of different blood drawing techniques (aspiration method and vacuum filling technique) used to draw blood from intravenous (IV) catheters in Emergency Department. Two techniques (aspiration vs. vacuum filling) used to draw blood into three different tubes (Sarstedt S-Monovette® 4.9 mL Serum Gel tube, BD 5 mL Vacutainer® Rapid Serum Tube (RST), and 5 mL Vacutainer® SST™II tube) and evaluated the effect of the hemolysis index of the sera on the tests analyzed...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775900/detection-of-toxoplasma-gondii-b1-gene-and-igm-in-igg-seropositive-pregnant-women
#4
K Sardarian, A H Maghsood, M Farimani, M Hajilooi, M Saidijam, Z Zarei Ghane, H Mahaki, A Zamani
BACKGROUND: The onset of acute toxoplasmosis in pregnant women may pose a risk to their growing fetuses. The timely diagnosis of infection in managing the disease and preventing its harmful consequences on the fetus is very important. Therefore, the study was conducted to identify acute toxoplasmosis in the pregnant women by detecting the specific IgM antibody and Toxoplasma gondii B1 gene. METHODS: A total of 653 serum samples of women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for IgG antibodies against Toxoplasma gondii by enzyme-linked immunosorbent as-say (ELISA)...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775899/performance-evaluation-of-allplex-respiratory-panels-1-2-and-3-for-the-detection-of-respiratory-viral-infection
#5
Jae Hee Lee, Jong Ho Lee
BACKGROUND: The Allplex Respiratory Panels 1, 2, and 3 (Allplex; Seegene, Republic of Korea) is a one-step real-time reverse transcription-PCR method based on the multiple detection temperature (MuDT) technology for the detection of respiratory viral infections. In this study, we evaluated the performance of the Allplex assay by comparing it with that of the Anyplex II RV16 detection kit (Anyplex; Seegene), a multiplex real-time PCR assay based on the tagging oligonucleotide cleavage and extension technology...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775898/elevated-plasma-d-dimer-in-adult-community-acquired-pneumonia-patients-is-associated-with-an-increased-inflammatory-reaction-and-lower-survival
#6
Yan L Ge, Cong H Liu, Nan Wang, Jing Xu, Xiao Y Zhu, Chuan S Su, Hui L Li, Hai F Zhang, Zhen Z Li, Hong L Li, Xue Zhang, Hao Chen, Hua L Yu, Ai S Fu, Hong Y Wang
BACKGROUND: Community-acquired pneumonia (CAP) is a common infectious disease. Inflammatory reaction and prognosis assessment in adult CAP patients are useful for CAP site of care decisions. Most CAP patients were diagnosed in an out-patient or emergency department, so a simple, cheap and rapidly available measurement to assess inflammatory reaction and prognosis has the prospect of broad application. The aim of this study was to investigate the usefulness of plasma D-Dimer in assessment of inflammatory reaction and prognosis in adult CAP patients...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775897/evaluation-of-the-serum-mac-2-binding-protein-glycosylation-isomer-test-used-for-diagnosis-and-monitoring-of-liver-fibrosis-and-the-correlation-of-mac-2-binding-protein-glycosylation-isomer-with-hemoglobin-a1c
#7
Rihwa Choi, Youngju Oh, Sukjung Lee, Sang Gon Lee
BACKGROUND: Our aims in this study were to evaluate the performance of the Mac-2 binding protein glycosylation isomer (M2BPGi), a liver fibrosis marker, using Sysmex HISCL following user verification guidelines before clinical use. We also evaluated the correlation between M2BPGi and hemoglobin A1c (HbA1c) because HbA1c is known as another biomarker associated with glycosylation. METHODS: The analytical performance of M2BPGi was verified following user verification guidelines by the Clinical and Laboratory Standards Institute...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775896/vascular-endothelial-growth-factor-d-vegf-d-is-elevated-in-bronchoalveolar-lavage-fluid-of-patients-with-lung-squamous-carcinoma
#8
Dan Lv, Lin Tan, Qihe Zhang, Hongying Ma, Yun Zhang, Qiaoli Zhang, Yiming Yu, Zhongbo Chen, Qunli Ding, Zaichun Deng
BACKGROUND: Previous studies have found that vascular endothelial growth factor (VEGF) is associated with lung cancer, yet little is known about vascular endothelial growth factor-D (VEGF-D) in bronchoalveolar lavage fluid (BALF) of lung cancer patients. In this study, we aim to investigate the expression and evaluation of VEGF-D in BALF for lung cancer diagnosis. METHODS: BALF samples were acquired from 81 patients: 40 with benign diseases and 41 with lung cancer...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775895/interference-rendering-capillary-electrophoresis-of-serum-proteins-uninterpretable
#9
Asmâa Biaz, Jean Uwingabiye, Elmostafa Benaissa, Emmanuel Millbank Owusu, Samira El Machtani Idrissi, Abdellah Dami, Zohra Ouzzif, Sanae Bouhsain
BACKGROUND: The authors report a clinical case where biological interference rendered the electrophoretic trace of serum proteins obtained by capillary electrophoresis as uninterpretable. METHODS: Electrophoresis of serum proteins and immunotyping immunoassay were performed on Capillary 2 Flex Piercing® Sebia. RESULTS: A 72-year-old male patient was hospitalized in the clinical hematology department for deep lymphadenopathies with hepatocellular failure...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775894/combined-laboratory-approach-to-detection-of-parvovirus-b19-and-coxiella-burnetii-in-patients-with-fever-of-unknown-origin
#10
Stefka Krumova, Adelina Pavlova, Kamelia Yotovska, Petia Genova-Kalou
BACKGROUND: Fever of unknown origin (FUO) is one of the greatest challenges for clinicians and patients. There are more than 200 etiological agents of FUO, among these the most common is the role of infection, neoplasms, and diseases of connective tissue. The aim of the present study is to investigate the role of the infectious agents parvovirus B19 (B19V) and Coxiella burnetii (C. burnetii) in the development of fever of unknown origin by a set of immunoenzymatic and molecular methods...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775893/evaluation-of-serum-alpha-fetoprotein-level-in-chronic-hepatitis-c-patients
#11
Na Yang, Zhuoran Li, Min Yan, Wende Xiao, Weihong Zhang, Youmin Long, Yingzi Cheng, Kaihua Ming, Banglao Xu
BACKGROUND: Hepatitis C virus (HCV) infection represents an important risk factor for hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a widely used biomarker for HCC. However, elevated serum AFP levels in different statuses of chronic hepatitis C (CHC) is not well defined. This study aimed to evaluate the relationship between AFP levels and HCV viral load in CHC patients. We also analyzed the correlation between three liver func-tion enzyme levels (AST, ALT, GGT) and HCV RNA viral load in CHC patients...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775892/the-association-of-d-dimer-and-spontaneous-hemorrhagic-ascites-with-outcomes-in-cirrhosis-a-retrospective-study
#12
He-Bin Fan, Xiao-Ling Tong, Xin-Yu Qian, Zhi Li
BACKGROUND: Cirrhotic patients with hemorrhagic ascites have significant morbidity and mortality. This study aims to determine the relationship between D-dimer values and hemorrhagic ascites in cirrhotic patients and analyze its predictive value. METHODS: This retrospective study screened 572 consecutive cirrhotic patients with ascites and hemorrhagic ascites (defined as red blood cells (RBC) in ascitic fluid ≥ 10,000/µL) during a 72-month period. The overall patient survival rate was measured by Kaplan-Meier analysis method...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775891/molecular-study-of-klebsiella-pneumoniae-virulence-genes-from-patients-with-hospital-acquired-sepsis
#13
Asmaa O Bakr And Maysaa E S Zaki
BACKGROUND: Klebsiella pneumoniae (K. pneumoniae) is a common pathogen associated with hospital acquired infections. The presence of virulence genes and antibiotic resistance genes are known risk factors for the infection by this bacterial species. METHODS: The aims of the present study were to i) investigate the coexistence of capsular genes of K. pneumoniae (rmpA and wcaG) with integron genes in clinical isolates of K. pneumoniae by polymerase chain reaction (PCR) from hospital acquired infections and to ii) correlate the presence of these genes with extended spectrum β-lactamase (ESBLs) and carbapenem-resistant phenotypes...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775890/comparison-of-the-genexpert%C3%A2-mtb-rif-test-and-conventional-methods-in-the-diagnosis-of-mycobacterium-tuberculosis
#14
Huseyin A Terzi, Ozlem Aydemir, Engin Karakece, Mehmet Koroglu, Mustafa Altindis
BACKGROUND: The objective of this study is to evaluate the performance of the GeneXpert MTB/RIF system in Mycobacterium tuberculosis (MTB) diagnosis and the detection of rifampicin resistance in pulmonary and extrapulmonary clinical samples. METHODS: A total of 849 samples (611 pulmonary and 238 extrapulmonary), which were sent to the laboratory of our hospital on suspicion of MTB, were included in the study. The samples cultured on Lowenstein Jensen medium and Mycobacteria Growth Indicator Tubes...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775889/an-evaluation-of-the-automated-cobas-u-701-microscopy-analyzer-for-the-routine-screening-of-urine-to-identify-negative-samples
#15
Natasha Waterhouse, Gillian Nichol, Dusanka Kasapic
BACKGROUND: Urinalysis based on microbiological culture and manual microscopy requires expertise and is labor intensive. Automated screening could save time and improve patient management in clinical settings. METHODS: We evaluated the fully automated cobas u 701 analyzer for identifying infection-negative urine samples using 2,046 anonymized samples from a routine pathology laboratory. Samples containing ≥ 40 white blood cells (WBC)/µL and/or ≥ 100 bacteria/µL were considered positive...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775888/an-improved-specimen-handling-procedure-for-pathogen-detection-of-the-cerebrospinal-fluid-by-microscope
#16
Hua-Cheng Wang, Li-Na Wang, Ya-Fan Ou-Yang, Yue-Feng Zhang
BACKGROUND: In order to increase the detection rate of pathogenic microorganisms in CSF, an improved specimen handling procedure (ISHP) was created. METHODS: This study enrolled encephalitis and control groups, both groups were handled with traditional specimen handling procedure (TSHP) and ISHP. Glutaraldehyde was added to the ISHP. Observed items included: total protein, glucose, chloride, adenosine deaminase, lactate dehydrogenase, sediment, cell and pathogen, Pandy's test...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775887/high-detection-rates-of-human-bocavirus-in-infants-and-small-children-with-lower-respiratory-tract-infection-from-croatia
#17
Sunčanica Ljubin-Sternak, Tomislav Meštrović, Irena Ivković-Jureković, Goran Tešović, Gordana Mlinarić-Galinović, Ivana Lukšić, Irena Tabain, Tatjana Tot, Maja Mijač, Jasmina Vraneš
BACKGROUND: Human bocavirus (HBoV) is known to cause lower respiratory tract infections (LRTI) in children and may result in substantial morbidity and mortality. The aim of this study was to determine HBoV prevalence among hospitalized infants and small children with acute LRTI in Zagreb, Croatia, as well as to evaluate HBoV DNA quantity in samples in relation to the patients' age and co-infection with other respiratory viruses. METHODS: During winter season 2016/2017, a total of 295 children younger than three years of age who were admitted to hospitals with LRTI were tested for the presence of HBoV, respiratory syncytial virus (RSV), adenovirus (ADV), parainfluenza virus (PIV) types 1 to 3, and human metapneumovirus (HMPV)...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775886/tuberculosis-antibody-and-tuberculin-positive-tests-initially-misdiagnosed-as-active-tuberculosis-in-a-broncholithiasis-patient-with-recurrent-hemoptysis-and-fever-proven-by-lung-lobectomy
#18
Yan L Ge, Cong H Liu, Meng H Wang, Li Q Li, Xiao Y Zhu, Zhen Z Li, Hong L Li, Hua L Yu, Qian Zhang, Zi Y Cui, Hai F Zhang, Xue Zhang, Ai-S Fu, Hong Y Wang
BACKGROUND: To report a case of broncholithiasis with recurrent hemoptysis and fever initially misdiagnosed as active tuberculosis. METHODS: The chest contrast-enhanced CT scan, electronic bronchoscope, and ultrathin bronchoscope were performed leading to the diagnosis of broncholithiasis, open lung lobectomy was done after thoracic surgery consultation. RESULTS: The chest contrast-enhanced CT scan showed a high-density intratracheal shadow and calcified lymph nodes...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775885/peripheral-blood-lymphocyte-to-monocyte-ratio-predicts-mortality-in-patients-with-hbv-related-decompensated-cirrhosis
#19
XiaoTing Qi
BACKGROUND: Recent reports suggest that the lymphocyte-to-monocyte ratio (LMR) is a potential biomarker for predicting clinical outcomes. In the present study, LMR in patients with hepatitis B virus-related decompensated cirrhosis (HBV-DeCi) was investigated to evaluate whether LMR may have utility as a new predictive marker for mortality in HBV-DeCi patients. METHODS: This was a retrospective cohort study that included 135 patients with HBV-DeCi. Logistic regression analysis and receiver operating characteristic curve were employed to assess the independent predictors for 1-month mortality rate of patients with HBV-DeCi...
January 1, 2019: Clinical Laboratory
https://read.qxmd.com/read/30775884/gestational-diabetes-mellitus-is-associated-with-plasma-amylase-in-a-chinese-pregnant-women-population
#20
Fan Yu, Wenjie Zhou, Xi Tan, Yongmei Jiang
BACKGROUND: The study aimed to investigate the relationship between plasma amylase levels and the endocrine and metabolic biomarkers in Chinese pregnant women with gestational diabetes mellitus (GDM) in the southwest of China and to compare plasma amylase with other known biomarkers in relation to their contributions to identifying GDM, with a view to establishing plasma amylase as an independent laboratory-based risk factor for GDM. METHODS: This study included 1,870 pregnant women divided into three groups: early pregnancy, middle pregnancy, and late pregnancy according to weeks of gestation, and 164 pregnant women were excluded by diseases...
January 1, 2019: Clinical Laboratory
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