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Journal of Industrial Microbiology & Biotechnology

Nataliia V Machushynets, Changsheng Wu, Somayah S Elsayed, Thomas Hankemeier, Gilles P van Wezel
Actinobacteria are a major source of novel bioactive natural products. A challenge in the screening of these microorganisms lies in finding the favorable growth conditions for secondary metabolite production and dereplication of known molecules. Here, we report that Streptomyces sp. MBT27 produces 4-quinazolinone alkaloids in response to elevated levels of glycerol, whereby quinazolinones A (1) and B (2) form a new sub-class of this interesting family of natural products. Global Natural Product Social molecular networking (GNPS) resulted in a quinazolinone-related network that included anthranilic acid (3), anthranilamide (4), 4(3H)-quinazolinone (5), and 2,2-dimethyl-1,2-dihydroquinazolin-4(3H)-one (6)...
February 7, 2019: Journal of Industrial Microbiology & Biotechnology
Rolf Müller, Gerard D Wright
No abstract text is available yet for this article.
February 7, 2019: Journal of Industrial Microbiology & Biotechnology
Richard H Baltz, Ikuro Abe, Eung-Soo Kim, Rolf Müller, Steven G Van Lanen, Gerry Wright
No abstract text is available yet for this article.
February 7, 2019: Journal of Industrial Microbiology & Biotechnology
Zhi-Qiang Xiong, Ling-Hui Kong, Hai-Lin Meng, Jin-Ming Cui, Yong-Jun Xia, Shi-Jie Wang, Lian-Zhong Ai
Streptococcus thermophilus is one of the most important homo-fermentative thermophilic bacteria, which is widely used as a starter culture in dairy industry. Both wild-type galactose-negative (Gal- ) S. thermophilus AR333 and galactose-positive (Gal+ ) S. thermophilus S-3 in this study were isolated from Chinese traditional dairy products. Here, to access the mechanism of the difference of galactose utilization between strains AR333 and S-3, the expression of gal-lac operons was examined using real-time qPCR in the presence of different sugars, and the gene organization of gal-lac operons was characterized using comparative genomics analysis...
February 4, 2019: Journal of Industrial Microbiology & Biotechnology
Lulu Song, Yefu Chen, Qinghuan Guo, Siyao Huang, Xuewu Guo, Dongguang Xiao
Beer foam stability, a key factor in evaluating overall beer quality, is influenced by proteinase A (PrA). Actin-severing protein cofilin and Golgi apparatus-localized Ca2+ ATPase Pmr1 are involved in protein sorting at the trans-Golgi network (TGN) in yeast Curwin et al. (Mol Biol Cell 23:2327-2338, 2012). To reduce PrA excretion into the beer fermentation broth, we regulated the Golgi apparatus sorting of PrA, thereby facilitating the delivery of more PrA to the vacuoles in the yeast cells. In the present study, the cofilin-coding gene COF1 and the Pmr1-coding gene PMR1 were overexpressed in the parental strain W303-1A and designated as W + COF1 and W + PMR1, respectively...
February 4, 2019: Journal of Industrial Microbiology & Biotechnology
Yang Zheng, Chen-Fan Sun, Yu Fu, Xin-Ai Chen, Yong-Quan Li, Xu-Ming Mao
Antibiotic production during secondary metabolism in Streptomyces spp. is elaborately controlled by multiple environmental signals and intracellular cascades. These include the two-component system PhoRP responding to phosphate starvation and a conserved signaling pathway mediated by the pleiotropic regulator AdpA. However, little information exists about how these two pathways work together for secondary metabolite production of Streptomyces. Herein, we report the dual regulation from the phosphate starvation-responsive regulator PhoP and AdpA on atrA promoter (atrAp) for the production of daptomycin, an antibiotic produced by Streptomyces roseosporus...
February 2, 2019: Journal of Industrial Microbiology & Biotechnology
Thu Thi Nguyen, In Yeub Hwang, Jeong Geol Na, Eun Yeol Lee
Propane is the main component of liquefied petroleum gas and is derived from crude oil processing. Methanotrophic bacteria can convert various alkanes using methane monooxygenase enzyme to primary alcohols. These are further oxidized to various aldehydes by alcohol dehydrogenases or methanol dehydrogenases. In this study, 2-propanol was produced from propane using the whole cells of Methylosinus trichosporium OB3b, Methylomicrobium alcaliphilum 20Z, and Methylomonas sp. DH-1 as the biocatalysts. The biocatalytic process of converting propane to 2-propanol was optimized by the use of several inhibitors and additives, such as EDTA, sodium phosphate, and sodium formate to prevent oxidation of 2-propanol to acetone and to enhance conversion of propane to propanol...
January 31, 2019: Journal of Industrial Microbiology & Biotechnology
Yanfang Zhao, Zhangqing Song, Zheng Ma, Andreas Bechthold, Xiaoping Yu
Rimocidin is a polyene macrolide that exhibits a strong inhibitory activity against a broad range of plant-pathogenic fungi. In this study, fermentation optimization and ribosome engineering technology were employed to enhance rimocidin production in Streptomyces rimosus M527. After the optimization of fermentation, rimocidin production in S. rimosus M527 increased from 0.11 ± 0.01 to 0.23 ± 0.02 g/L during shake-flask experiments and reached 0.41 ± 0.05 g/L using 5-L fermentor. Fermentation optimization was followed by the generation of mutants of S...
January 29, 2019: Journal of Industrial Microbiology & Biotechnology
Han Wang, Han-Xiang Li, Fang Fang, Jin-Song Guo, You-Peng Chen, Pen Yan, Ji-Xiang Yang
Dealing with nitrogen-rich saline wastewater produced by industries remains challenging because of the inhibition of functional microorganisms by high salinity. The underlying mechanisms of anaerobic ammonium-oxidizing bacteria (AnAOB) exposed to salinity stress should be studied to investigate the potential of anaerobic ammonium oxidation (ANAMMOX) for applications in such wastewater. In this study, the total DNA from granular sludge was extracted from an expanded granular sludge bed (EGSB) reactor operated at 0, 15 and 30 g/L salinity and subjected to high-throughput sequencing...
January 28, 2019: Journal of Industrial Microbiology & Biotechnology
Mohammad R Seyedsayamdost
Bacterial metabolism is comprised of primary metabolites, the intracellular molecules of life that enable growth and proliferation, and secondary metabolites, predominantly extracellular molecules that facilitate a microbe's interaction with its environment. While our knowledge of primary metabolism and its web of interconnected intermediates is quantitative and holistic, significant knowledge gaps remain in our understanding of the secondary metabolomes of bacteria. In this Perspective, I discuss the main challenges involved in obtaining a global, comprehensive picture of bacterial secondary metabolomes, specifically in biosynthetically "gifted" microbes...
January 25, 2019: Journal of Industrial Microbiology & Biotechnology
F P Jake Haeckl, João L Baldim, Dasha Iskakova, Kenji L Kurita, Marisi G Soares, Roger G Linington
The genus Burkholderia is an emerging source of novel natural products chemistry, yet to date few methods exist for the selective isolation of strains of this genus from the environment. More broadly, tools to efficiently design selection media for any given genus would be of significant value to the natural products and microbiology communities. Using a modification of the recently published SMART protocol, we have developed a two-stage isolation protocol for strains from the genus Burkholderia. This method uses a combination of selective agar isolation media and multiplexed PCR profiling to derive Burkholderia strains from environmental samples with 95% efficiency...
January 24, 2019: Journal of Industrial Microbiology & Biotechnology
Aureliano Claret da Cunha, Lorena Soares Gomes, Fernanda Godoy-Santos, Fábio Faria-Oliveira, Janaína Aparecida Teixeira, Geraldo Magela Santos Sampaio, Maria José Magalhães Trópia, Ieso Miranda Castro, Cândida Lucas, Rogelio Lopes Brandão
Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S...
January 24, 2019: Journal of Industrial Microbiology & Biotechnology
Matt McErlean, Jonathan Overbay, Steven Van Lanen
Nonribosomal peptide synthetases (NRPSs) are involved in the biosynthesis of numerous peptide and peptide-like natural products that have been exploited in medicine, agriculture, and biotechnology, among other fields. As a consequence, there have been considerable efforts aimed at understanding how NRPSs orchestrate the assembly of these natural products. This review highlights several recent examples that continue to expand upon the fundamental knowledge of NRPS mechanism and includes (1) the discovery of new NRPS substrates and the mechanism by which these sometimes structurally complex substrates are made, (2) the characterization of new NRPS activities and domains that function during the process of peptide assembly, and (3) the various catalytic strategies that are utilized to release the NRPS product...
January 23, 2019: Journal of Industrial Microbiology & Biotechnology
Dehu Sun, Jiuzhou Chen, Yu Wang, Mingyue Li, Deming Rao, Yanmei Guo, Ning Chen, Ping Zheng, Jibin Sun, Yanhe Ma
Corynebacterium glutamicum is an important platform strain that is wildly used in industrial production of amino acids and various other biochemicals. However, due to good genomic stability, C. glutamicum is more difficult to engineer than genetically tractable hosts. Herein, a synthetic small regulatory RNA (sRNA)-based gene knockdown strategy was developed for C. glutamicum. The RNA chaperone Hfq from Escherichia coli and a rationally designed sRNA consisting of the E. coli MicC scaffold and a target binding site were proven to be indispensable for repressing green fluorescent protein expression in C...
January 22, 2019: Journal of Industrial Microbiology & Biotechnology
Dipesh Dhakal, Vijay Rayamajhi, Ravindra Mishra, Jae Kyung Sohng
Nocardia spp. are catalase positive, aerobic, and non-motile Gram-positive filamentous bacteria. Many Nocarida spp. have been reported as unusual causes of diverse clinical diseases in both humans and animals. Therefore, they have been studied for a long time, primarily focusing on strain characterization, taxonomic classification of new isolates, and host pathophysiology. Currently, there are emerging interests in isolating bioactive molecules from diverse actinobacteria including Nocardia spp. and studying their biosynthetic mechanisms...
January 18, 2019: Journal of Industrial Microbiology & Biotechnology
Maximilian J Helf, Michael F Freeman, Jörn Piel
Polytheonamides are the most extensively modified ribosomally synthesized and post-translationally modified peptide natural products (RiPPs) currently known. In RiPP biosynthesis, the processed peptide is usually released from a larger precursor by proteolytic cleavage to generate the bioactive terminal product of the pathway. For polytheonamides, which are members of a new RiPP family termed proteusins, we have recently shown that such cleavage is catalyzed by the cysteine protease PoyH acting on the precursor PoyA, both encoded in the polytheonamide biosynthetic gene cluster...
January 9, 2019: Journal of Industrial Microbiology & Biotechnology
De-Qiang Zhu, Jian-Rong Wu, Xiao-Bei Zhan, Li Zhu, Yun Jiang
Typical N-acetyl-D-neuraminic acid (Neu5Ac) production uses N-acetyl-D-glucosamine (GlcNAc) and excess pyruvate as substrates in the enzymatic or whole-cell biocatalysis process. In a previous study, a Neu5Ac-producing biocatalytic process via engineered Escherichia coli SA-05/pDTrc-AB/pCDF-pck-ppsA was constructed without exogenous pyruvate. In this study, glycerol was found to be a good energy source compared with glucose for the catalytic system with resting cells, and Neu5Ac production increased to 13.97 ± 0...
January 8, 2019: Journal of Industrial Microbiology & Biotechnology
Jianjun Pei, Anna Chen, Ping Dong, Xuejia Shi, Linguo Zhao, Fuliang Cao, Feng Tang
Kaempferol and astragalin are used as standards to assess the quality of Ginkgo biloba extract and Radix astragali, respectively, and possess numerous biological properties. In this study, we constructed a recombinant strain with a highly efficient biosynthetic pathway of kaempferol by screening key enzyme genes, designing a synthetic fusion enzyme and increasing the gene copy number. By optimizing conversion and fed-batch fermentation conditions, maximal kaempferol production reached 1184.2 ± 16.5 mg/L, which represents the highest yield of kaempferol from naringenin reported to date...
January 7, 2019: Journal of Industrial Microbiology & Biotechnology
Rasmus Villebro, Simon Shaw, Kai Blin, Tilmann Weber
The software antiSMASH examines microbial genome data to identify and analyze biosynthetic gene clusters for a wide range of natural products. So far, type II polyketide synthase (PKS) gene clusters could only be identified, but no detailed predictions for type II PKS gene clusters could be provided. In this study, an antiSMASH module for analyzing type II PKS gene clusters has been developed. The module detects genes/proteins in the type II PKS gene cluster involved with polyketide biosynthesis and is able to make predictions about the aromatic polyketide product...
January 4, 2019: Journal of Industrial Microbiology & Biotechnology
Nigel J Mouncey, Hiroshi Otani, Daniel Udwary, Yasuo Yoshikuni
Natural products are a large family of diverse and complex chemical molecules that have roles in both primary and secondary metabolism, and over 210,000 natural products have been described. Secondary metabolite natural products are of high commercial and societal value with therapeutic uses as antibiotics, antifungals, antitumor and antiparasitic products and in agriculture as products for crop protection and animal health. There is a resurgence of activity in exploring natural products for a wide range of applications, due to not only increasing antibiotic resistance, but the advent of next-generation genome sequencing and new technologies to interrogate and investigate natural product biosynthesis...
January 4, 2019: Journal of Industrial Microbiology & Biotechnology
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