journal
Journals Cellular and Molecular Life Sc...

Cellular and Molecular Life Sciences : CMLS

https://read.qxmd.com/read/36952129/restriction-of-access-to-the-central-cavity-is-a-major-contributor-to-substrate-selectivity-in-plant-abcg-transporters
#1
JOURNAL ARTICLE
Konrad Pakuła, Carlos Sequeiros-Borja, Wanda Biała-Leonhard, Aleksandra Pawela, Joanna Banasiak, Aurélien Bailly, Marcin Radom, Markus Geisler, Jan Brezovsky, Michał Jasiński
ABCG46 of the legume Medicago truncatula is an ABC-type transporter responsible for highly selective translocation of the phenylpropanoids, 4-coumarate, and liquiritigenin, over the plasma membrane. To investigate molecular determinants of the observed substrate selectivity, we applied a combination of phylogenetic and biochemical analyses, AlphaFold2 structure prediction, molecular dynamics simulations, and mutagenesis. We discovered an unusually narrow transient access path to the central cavity of MtABCG46 that constitutes an initial filter responsible for the selective translocation of phenylpropanoids through a lipid bilayer...
March 23, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36952018/podocyte-specific-deletion-of-ubiquitin-carboxyl-terminal-hydrolase-l1-causes-podocyte-injury-by-inducing-endoplasmic-reticulum-stress
#2
JOURNAL ARTICLE
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Adiljan Abdurusul, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a unique component of the ubiquitin-proteasome system (UPS), which has multiple activities in maintaining intracellular ubiquitin levels. We previously reported the aberrant low expression of UCHL1 in podocytes of non-immune complex-mediated glomerulonephritis, and recent studies indicate that anti-UCHL1 antibody was responsible for the refractory minimal change disease (MCD), but the specific effect of UCHL1 to the podocytopathy has not been determined. Therefore, we generated podocyte-specific UCHL1 gene knockout (UCHL1cre/cre ) rats model...
March 23, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36947256/receptor-tyrosine-kinase-inhibitors-in-cancer
#3
REVIEW
Nasim Ebrahimi, Elmira Fardi, Hajarossadat Ghaderi, Sahar Palizdar, Roya Khorram, Reza Vafadar, Masoud Ghanaatian, Fatemeh Rezaei-Tazangi, Payam Baziyar, Amirhossein Ahmadi, Michael R Hamblin, Amir Reza Aref
Targeted therapy is a new cancer treatment approach, involving drugs that particularly target specific proteins in cancer cells, such as receptor tyrosine kinases (RTKs) which are involved in promoting growth and proliferation, Therefore inhibiting these proteins could impede cancer progression. An understanding of RTKs and the relevant signaling cascades, has enabled the development of many targeted drug therapies employing RTK inhibitors (RTKIs) some of which have entered clinical application. Here we discuss RTK structures, activation mechanisms and functions...
March 22, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36941472/integrative-epigenetic-analysis-reveals-ap-1-promotes-activation-of-tumor-infiltrating-regulatory-t-cells-in-hcc
#4
JOURNAL ARTICLE
Baowen Zhuo, Qifan Zhang, Tingyan Xie, Yidan Wang, Zhengliang Chen, Daming Zuo, Bo Guo
Regulatory T (Treg) cells that infiltrate human tumors exhibit stronger immunosuppressive activity compared to peripheral blood Treg cells (PBTRs), thus hindering the induction of effective antitumor immunity. Previous transcriptome studies have identified a set of genes that are conserved in tumor-infiltrating Treg cells (TITRs). However, epigenetic profiles of TITRs have not yet been completely deciphered. Here, we employed ATAC-seq and CUT&Tag assays to integrate transcriptome profiles and identify functional regulatory elements in TITRs...
March 20, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36939886/the-forkhead-box-o3-foxo3-a-key-player-in-the-regulation-of-ischemia-and-reperfusion-injury
#5
REVIEW
Moussa Omorou, Yiwei Huang, Meng Gao, Chenxi Mu, Weijing Xu, Yuchun Han, Hui Xu
Forkhead box O3 is a protein encoded by the FOXO3 gene expressed throughout the body. FOXO3 could play a crucial role in longevity and many other pathologies, such as Alzheimer's disease, glioblastoma, and stroke. This study is a comprehensive review of the expression of FOXO3 under ischemia and reperfusion (IR) and the molecular mechanisms of its regulation and function. We found that the expression level of FOXO3 under ischemia and IR is tissue-specific. Specifically, the expression level of FOXO3 is increased in the lung and intestinal epithelial cells after IR...
March 20, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36935456/pharmacological-and-mechanistic-study-of-ps1-a-pdia4-inhibitor-in-%C3%AE-cell-pathogenesis-and-diabetes-in-db-db-mice
#6
JOURNAL ARTICLE
Hui-Ju Tseng, Wen-Chu Chen, Tien-Fen Kuo, Greta Yang, Ching-Shan Feng, Hui-Ming Chen, Tzung-Yan Chen, Tsung-Han Lee, Wen-Chin Yang, Keng-Chang Tsai, Wei-Jan Huang
Pdia4 has been characterized as a key protein that positively regulates β-cell failure and diabetes via ROS regulation. Here, we investigated the function and mechanism of PS1, a Pdia4 inhibitor, in β-cells and diabetes. We found that PS1 had an IC50 of 4 μM for Pdia4. Furthermore, PS1 alone and in combination with metformin significantly reversed diabetes in db/db mice, 6 to 7 mice per group, as evidenced by blood glucose, glycosylated hemoglobin A1c (HbA1c ), glucose tolerance test, diabetic incidence, survival and longevity (P < 0...
March 19, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36933158/lncrna-mir4458hg-modulates-hepatocellular-carcinoma-progression-by-activating-m6a-dependent-glycolysis-and-promoting-the-polarization-of-tumor-associated-macrophages
#7
JOURNAL ARTICLE
Ying Ye, Menghan Wang, Guoyu Wang, Zhongchao Mai, Borong Zhou, Yang Han, Juhua Zhuang, Wei Xia
Long non-coding RNAs (lncRNAs) play significant roles in different biological functions of cancers. However, their function in the metabolism of glucose in patients with human hepatocellular carcinoma (HCC) remains largely unknown. In this study, HCC and paired intact liver tissues were utilized to examine the miR4458HG expression using qRT-PCR and human HCC cell lines to examine cell proliferation, colony formation, and glycolysis after transfection of siRNAs targeting miR4458HG or miR4458HG vectors. The molecular mechanism of miR4458HG was clarified through in situ hybridization, Western blotting, qRT-PCR, RNA pull-down, and RNA immunoprecipitation analysis...
March 18, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36933062/loss-of-kmt2c-in-vivo-leads-to-emt-mitochondrial-dysfunction-and-improved-response-to-lapatinib-in-breast-cancer
#8
JOURNAL ARTICLE
Nikiana Simigdala, Anna Chalari, Aimilia D Sklirou, Evangelia Chavdoula, George Papafotiou, Pelagia Melissa, Aimilia Kafalidou, Nikolaos Paschalidis, Ioannis S Pateras, Emmanouil Athanasiadis, Dimitris Konstantopoulos, Ioannis P Trougakos, Apostolos Klinakis
Deep sequencing of human tumours has uncovered a previously unappreciated role for epigenetic regulators in tumorigenesis. H3K4 methyltransferase KMT2C/MLL3 is mutated in several solid malignancies, including more than 10% of breast tumours. To study the tumour suppressor role of KMT2C in breast cancer, we generated mouse models of Erbb2/Neu, Myc or PIK3CA-driven tumorigenesis, in which the Kmt2c locus is knocked out specifically in the luminal lineage of mouse mammary glands using the Cre recombinase. Kmt2c knock out mice develop tumours earlier, irrespective of the oncogene, assigning a bona fide tumour suppressor role for KMT2C in mammary tumorigenesis...
March 18, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36932186/endogenous-but-not-sensory-driven-activity-controls-migration-morphogenesis-and-survival-of-adult-born-juxtaglomerular-neurons-in-the-mouse-olfactory-bulb
#9
JOURNAL ARTICLE
Kaizhen Li, Katherine Figarella, Xin Su, Yury Kovalchuk, Jessika Gorzolka, Jonas J Neher, Nima Mojtahedi, Nicolas Casadei, Ulrike B S Hedrich, Olga Garaschuk
The development and survival of adult-born neurons are believed to be driven by sensory signaling. Here, in vivo analyses of motility, morphology and Ca2+ signaling, as well as transcriptome analyses of adult-born juxtaglomerular cells with reduced endogenous excitability (via cell-specific overexpression of either Kv1.2 or Kir2.1 K+ channels), revealed a pronounced impairment of migration, morphogenesis, survival, and functional integration of these cells into the mouse olfactory bulb, accompanied by a reduction in cytosolic Ca2+ fluctuations, phosphorylation of CREB and pCREB-mediated gene expression...
March 18, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36930354/inhibition-of-lxr-controls-the-polarization-of-human-inflammatory-macrophages-through-upregulation-of-mafb
#10
JOURNAL ARTICLE
Arturo González de la Aleja, Cristina Herrero, Mónica Torres-Torresano, María Teresa Schiaffino, Alejandro Del Castillo, Bárbara Alonso, Miguel A Vega, Amaya Puig-Kröger, Antonio Castrillo, Ángel L Corbí
Monocyte-derived macrophages contribute to pathogenesis in inflammatory diseases and their effector functions greatly depend on the prevailing extracellular milieu. Whereas M-CSF primes macrophages for acquisition of an anti-inflammatory profile, GM-CSF drives the generation of T cell-stimulatory and pro-inflammatory macrophages. Liver X Receptors (LXRα and LXRβ) are nuclear receptors that control cholesterol metabolism and regulate differentiation of tissue-resident macrophages. Macrophages from rheumatoid arthritis and other inflammatory pathologies exhibit an enriched LXR pathway, and recent reports have shown that LXR activation raises pro-inflammatory effects and impairs the acquisition of the anti-Inflammatory profile of M-CSF-dependent monocyte-derived macrophages (M-MØ)...
March 17, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36930324/correction-chip-induces-ubiquitination-and-degradation-of-hmgb1-to-regulate-glycolysis-in-ovarian-endometriosis
#11
Yujun Sun, Qian Wang, Mengxue Wang, Fangyuan Sun, Pengyun Qiao, Aifang Jiang, Chune Ren, Zhenhai Yu, Tingting Yang
No abstract text is available yet for this article.
March 17, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36930302/the-%C3%AE-secretase-derived-app-fragment-%C3%AE-ctf-is-localized-in-golgi-endosomes-and-extracellular-vesicles-and-contributes-to-a%C3%AE-production
#12
JOURNAL ARTICLE
Elissa Afram, Inger Lauritzen, Alexandre Bourgeois, Wejdane El Manaa, Eric Duplan, Mounia Chami, Audrey Valverde, Bauer Charlotte, Raphaëlle Pardossi-Piquard, Frederic Checler
The processing of the amyloid precursor protein (APP) is one of the key events contributing to Alzheimer's disease (AD) etiology. Canonical cleavages by β- and γ-secretases lead to Aβ production which accumulate in amyloid plaques. Recently, the matrix metalloprotease MT5-MMP, referred to as η-secretase, has been identified as a novel APP cleaving enzyme producing a transmembrane fragment, ηCTF that undergoes subsequent cleavages by α- and β-secretases yielding the Aηα and Aηβ peptides, respectively...
March 17, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36930291/aggregation-prone-tdp-43-sequesters-and-drives-pathological-transitions-of-free-nuclear-tdp-43
#13
JOURNAL ARTICLE
Sean S Keating, Adekunle T Bademosi, Rebecca San Gil, Adam K Walker
Aggregation of the RNA-binding protein, TDP-43, is the unifying hallmark of amyotrophic lateral sclerosis and frontotemporal dementia. TDP-43-related neurodegeneration involves multiple changes to normal physiological TDP-43, which undergoes nuclear depletion, cytoplasmic mislocalisation, post-translational modification, and aberrant liquid-liquid phase separation, preceding inclusion formation. Along with toxic cytoplasmic aggregation, concurrent depletion and dysfunction of normal nuclear TDP-43 in cells with TDP-43 pathology is likely a key potentiator of neurodegeneration, but is not well understood...
March 17, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36929461/microstructured-soft-devices-for-the-growth-and-analysis-of-populations-of-homogenous-multicellular-tumor-spheroids
#14
JOURNAL ARTICLE
Ottavia Tartagni, Alexandra Borók, Emanuela Mensà, Attila Bonyár, Barbara Monti, Johan Hofkens, Anna Maria Porcelli, Giampaolo Zuccheri
Multicellular tumor spheroids are rapidly emerging as an improved in vitro model with respect to more traditional 2D culturing. Microwell culturing is a simple and accessible method for generating a large number of uniformly sized spheroids, but commercially available systems often do not enable researchers to perform complete culturing and analysis pipelines and the mechanical properties of their culture environment are not commonly matching those of the target tissue. We herein report a simple method to obtain custom-designed self-built microwell arrays made of polydimethylsiloxane or agarose for uniform 3D cell structure generation...
March 16, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36928776/ube2t-resolves-transcription-replication-conflicts-and-protects-common-fragile-sites-in-primordial-germ-cells
#15
JOURNAL ARTICLE
Yongze Yu, Weiwei Xu, Canxin Wen, Simin Zhao, Guangyu Li, Ran Liu, Zi-Jiang Chen, Yingying Qin, Jinlong Ma, Yajuan Yang, Shidou Zhao
The proper development of primordial germ cells (PGCs) is an essential prerequisite for gametogenesis and mammalian fertility. The Fanconi anemia (FA) pathway functions in maintaining the development of PGCs. FANCT/UBE2T serves as an E2 ubiquitin-conjugating enzyme that ubiquitylates the FANCD2-FANCI complex to activate the FA pathway, but its role in the development of PGCs is not clear. In this study, we found that Ube2t knockout mice showed defects in PGC proliferation, leading to severe loss of germ cells after birth...
March 16, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36928770/kif13b-mediates-vegfr2-recycling-to-modulate-vascular-permeability
#16
JOURNAL ARTICLE
Hyun-Dong Cho, Nguyễn Thị Thanh Nhàn, Christopher Zhou, Kayeman Tu, Tara Nguyen, Nicolene A Sarich, Kaori H Yamada
Excessive vascular endothelial growth factor-A (VEGF-A) signaling induces vascular leakage and angiogenesis in diseases. VEGFR2 trafficking to the cell surface, mediated by kinesin-3 family protein KIF13B, is essential to respond to VEGF-A when inducing angiogenesis. However, the precise mechanism of how KIF13B regulates VEGF-induced signaling and its effects on endothelial permeability is largely unknown. Here we show that KIF13B-mediated recycling of internalized VEGFR2 through Rab11-positive recycling vesicle regulates endothelial permeability...
March 16, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36922433/extracellular-vesicles-from-hyperammonemic-rats-induce-neuroinflammation-in-hippocampus-and-impair-cognition-in-control-rats
#17
JOURNAL ARTICLE
Paula Izquierdo-Altarejos, Mar Martínez-García, Vicente Felipo
Patients with liver cirrhosis show hyperammonemia and peripheral inflammation and may show hepatic encephalopathy with cognitive impairment, reproduced by rats with chronic hyperammonemia. Peripheral inflammation induces neuroinflammation in hippocampus of hyperammonemic rats, altering neurotransmission and leading to cognitive impairment. Extracellular vesicles (EVs) may transmit pathological effects from the periphery to the brain. We hypothesized that EVs from peripheral blood would contribute to cognitive alterations in hyperammonemic rats...
March 15, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36920550/mesenchyme-derived-vertebrate-lonesome-kinase-controls-lung-organogenesis-by-altering-the-matrisome
#18
JOURNAL ARTICLE
Salome M Brütsch, Elizabeta Madzharova, Sophia Pantasis, Till Wüstemann, Selina Gurri, Heiko Steenbock, Amiq Gazdhar, Gisela Kuhn, Peter Angel, Saverio Bellusci, Jürgen Brinckmann, Ulrich Auf dem Keller, Sabine Werner, Mattia R Bordoli
Vertebrate lonesome kinase (VLK) is the only known secreted tyrosine kinase and responsible for the phosphorylation of a broad range of secretory pathway-resident and extracellular matrix proteins. However, its cell-type specific functions in vivo are still largely unknown. Therefore, we generated mice lacking the VLK gene (protein kinase domain containing, cytoplasmic (Pkdcc)) in mesenchymal cells. Most of the homozygous mice died shortly after birth, most likely as a consequence of their lung abnormalities and consequent respiratory failure...
March 15, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36917323/aav-net1-facilitates-the-trans-differentiation-of-supporting-cells-into-hair-cells-in-the-murine-cochlea
#19
JOURNAL ARTICLE
Liyan Zhang, Yuan Fang, Fangzhi Tan, Fangfang Guo, Ziyu Zhang, Nianci Li, Qiuhan Sun, Jieyu Qi, Renjie Chai
Mechanosensitive hair cells (HCs) in the cochlear sensory epithelium are critical for sound detection and transduction. Mammalian HCs in the cochlea undergo cytogenesis during embryonic development, and irreversible damage to hair cells postnatally is a major cause of deafness. During the development of the organ of Corti, HCs and supporting cells (SCs) originate from the same precursors. In the neonatal cochlea, damage to HCs activates adjacent SCs to act as HC precursors and to differentiate into new HCs...
March 14, 2023: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/36917314/brain-washing-and-neural-health-role-of-age-sleep-and-the-cerebrospinal-fluid-melatonin-rhythm
#20
REVIEW
Russel J Reiter, Ramaswamy Sharma, Maira Smaniotto Cucielo, Dun Xian Tan, Sergio Rosales-Corral, Giuseppe Gancitano, Luiz Gustavo de Almeida Chuffa
The brain lacks a classic lymphatic drainage system. How it is cleansed of damaged proteins, cellular debris, and molecular by-products has remained a mystery for decades. Recent discoveries have identified a hybrid system that includes cerebrospinal fluid (CSF)-filled perivascular spaces and classic lymph vessels in the dural covering of the brain and spinal cord that functionally cooperate to remove toxic and non-functional trash from the brain. These two components functioning together are referred to as the glymphatic system...
March 14, 2023: Cellular and Molecular Life Sciences: CMLS
journal
journal
32709
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.