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Cell Biochemistry and Biophysics | Page 3

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https://read.qxmd.com/read/29313175/a-biomolecular-network-driven-proteinic-interaction-in-hcv-clearance
#41
Pratichi Singh, Febin Prabhu Dass J
Hepatitis C virus infection causes chronic liver disease that leads to cancer-related mortality. Presently around 30% of the HCV (infected) affected population get rid of the infection through spontaneous disease clearance. This phenomenon is conducted by a set of reported immune candidate genes. Hence, this study focuses only on these immune-response related genes with aid of network approach, where the idea is to disseminate the network for better understanding of key functional genes and their transcription control activity...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29134494/expression-of-telomere-associated-proteins-is-interdependent-to-stabilize-native-telomere-structure-and-telomere-dysfunction-by-g-quadruplex-ligand-causes-terra-upregulation
#42
Ratan Sadhukhan, Priyanka Chowdhury, Sourav Ghosh, Utpal Ghosh
Telomere DNA can form specialized nucleoprotein structure with telomere-associated proteins to hide free DNA ends or G-quadruplex structures under certain conditions especially in presence of G-quadruplex ligand. Telomere DNA is transcribed to form non-coding telomere repeat-containing RNA (TERRA) whose biogenesis and function is poorly understood. Our aim was to find the role of telomere-associated proteins and telomere structures in TERRA transcription. We silenced four [two shelterin (TRF1, TRF2) and two non-shelterin (PARP-1, SLX4)] telomere-associated genes using siRNA and verified depletion in protein level...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29116599/the-class-a-gpcr-dopamine-d2-receptor-forms-transient-dimers-stabilized-by-agonists-detection-by-single-molecule-tracking
#43
Rinshi S Kasai, Shuichi V Ito, Ryo M Awane, Takahiro K Fujiwara, Akihiro Kusumi
Whether class-A G-protein coupled receptors (GPCRs) exist and work as monomers or dimers has drawn extensive attention. A class-A GPCR dopamine D2 receptor (D2R) is involved in many physiological and pathological processes and diseases, indicating its critical role in proper functioning of neuronal circuits. In particular, D2R homodimers might play key roles in schizophrenia development and amphetamine-induced psychosis. Here, using single-molecule imaging, we directly tracked single D2R molecules in the plasma membrane at a physiological temperature of 37 °C, and unequivocally determined that D2R forms transient dimers with a lifetime of 68 ms in its resting state...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29090414/liver-genes-expression-induced-by-tamoxifen-loaded-solid-lipid-nanoparticles-in-wistar-female-rats
#44
Naser Zanganeh, Nasrin Ziamajidi, Iraj Khodadadi, Massoud Saidijam, Roghayeh Abbasalipourkabir
The objective of this study was to investigate the effect of free tamoxifen and tamoxifen-loaded solid lipid nanoparticles (SLN) on cytochrome P450 (CYP3A2) and flavin-containing monooxygenase1 (FMO1) genes expression in the liver of female Wistar rats. Thirty female Wistar rats aged 7-8 weeks, were divided into six groups of six rats each. The first, second, third, and fourth groups were ovariectomized and received tamoxifen (2  mg/kg of body weight dissolved in 1  ml olive oil), tamoxifen-loaded SLN (2  mg/kg of body weight dispersed in 1  ml olive oil), SLN (10  mg/kg of body weight dispersed in 1  ml olive oil), and 1  ml olive oil, respectively...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29067585/intrinsic-response-towards-physiologic-stiffness-is-cell-type-dependent
#45
Michael Reimer, Silviya Petrova Zustiak, Saahil Sheth, Joseph Martin Schober
In the continuous search for better tissue engineering scaffolds it has become increasingly clear that the substrate properties dramatically affect cell responses. Here we compared cells from a physiologically stiff tissue, melanoma, to cells isolated from a physiologically soft tissue, brain. We measured the cell line responses to laminin immobilized onto glass or polyacrylamide hydrogels tuned to have a Young's modulus ranging from 1 to 390 kPa. Single cells were analyzed for spreading area, shape, total actin content, actin-based morphological features and modification of immobilized laminin...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29039057/the-effects-of-low-doses-of-gamma-radiation-on-growth-and-membrane-activity-of-pseudomonas-aeruginosa-grp3-and-escherichia-coli-m17
#46
D Soghomonyan, A Margaryan, K Trchounian, K Ohanyan, H Badalyan, A Trchounian
Microorganisms are part of the natural environments and reflect the effects of different physical factors of surrounding environment, such as gamma (γ) radiation. This work was devoted to the study of the influence of low doses of γ radiation with the intensity of 2.56 μW (m2  s)-1 (absorbed doses were 3.8 mGy for the radiation of 15 min and 7.2 mGy-for 30 min) on Escherichia coli M-17 and Pseudomonas aeruginosa GRP3 wild type cells. The changes of bacterial, growth, survival, morphology, and membrane activity had been studied after γ irradiation...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28975596/erratum-to-hypoxia-augments-increased-hif-1a-and-reduced-survival-protein-p-akt-in-gelsolin-gsn-dependent-cardiomyoblast-cell-apoptosis
#47
Yu-Lan Yeh, Wei-Syun Hu, Wei-Jen Ting, Chia-Yao Shen, Hsi-Hsien Hsu, Li-Chin Chung, Chuan-Chou Tu, Sheng-Huang Chang, Cecilia-Hsuan Day, Yuhsin Tsai, Chih-Yang Huang
No abstract text is available yet for this article.
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28952073/cysteine-to-serine-conversion-at-111th-position-renders-the-disaggregation-and-retains-the-stabilization-of-detrimental-sod1-a4v-mutant-against-amyotrophic-lateral-sclerosis-in-human-a-discrete-molecular-dynamics-study
#48
E Srinivasan, R Rajasekaran
Protein aggregation is a hallmark of various neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) in humans. Mutations in Cu/Zn superoxide dismutase (SOD1) protein were found to be a prominent cause behind the majority of the familial ALS cases with abnormal protein aggregates. Herein, we report the biophysical characterization of the beneficial mutation C111S that stabilizes the SOD1 harboring A4V mutation, one of the most lethal diseases causing mutant that leads to protein destabilization and aggregation...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28942575/a-contact-based-method-for-differentiation-of-human-mesenchymal-stem-cells-into-an-endothelial-cell-phenotype
#49
Binata Joddar, Shweta Anil Kumar, Alok Kumar
Adult stem cells such as mesenchymal stem cells (MSC) are known to possess the ability to augment neovascularization processes and are thus widely popular as an autologous source of progenitor cells. However there is a huge gap in our current knowledge of mechanisms involved in differentiating MSC into endothelial cells (EC), essential for lining engineered blood vessels. To fill up this gap, we attempted to differentiate human MSC into EC, by culturing the former onto chemically fixed layers of EC or its ECM, respectively...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28871484/epr-oximetry-sensor-developing-a-tam-derivative-for-in-vivo-studies
#50
Agnieszka Boś-Liedke, Magdalena Walawender, Anna Woźniak, Dorota Flak, Jacek Gapiński, Stefan Jurga, Małgorzata Kucińska, Adam Plewiński, Marek Murias, Marwa Elewa, Lisa Lampp, Peter Imming, Krzysztof Tadyszak
Oxygenation is one of the most important physiological parameters of biological systems. Low oxygen concentration (hypoxia) is associated with various pathophysiological processes in different organs. Hypoxia is of special importance in tumor therapy, causing poor response to treatment. Triaryl methyl (TAM) derivative radicals are commonly used in electron paramagnetic resonance (EPR) as sensors for quantitative spatial tissue oxygen mapping. They are also known as magnetic resonance imaging (MRI) contrast agents and fluorescence imaging compounds...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28852971/identification-of-phytochemicals-targeting-c-met-kinase-domain-using-consensus-docking-and-molecular-dynamics-simulation-studies
#51
Shima Aliebrahimi, Shideh Montasser Kouhsari, Seyed Nasser Ostad, Seyed Shahriar Arab, Leila Karami
c-Met receptor tyrosine kinase is a proto-oncogene whose aberrant activation is attributed to a lower rate of survival in most cancers. Natural product-derived inhibitors known as "fourth generation inhibitors" constitute more than 60% of anticancer drugs. Furthermore, consensus docking approach has recently been introduced to augment docking accuracy and reduce false positives during a virtual screening. In order to obtain novel small-molecule Met inhibitors, consensus docking approach was performed using Autodock Vina and Autodock 4...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28831668/fish-collagen-hydrolysates-modulate-cartilage-metabolism
#52
Kanchanit Boonmaleerat, Orawan Wanachewin, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
Osteoarthritis is a degenerative joint disease in which interleukin-1β plays a major role in the inflammatory process. Administration of collagen hydrolysate was an optional treatment of osteoarthritis. Fish has become an interesting source of collagen hydrolysate because of religious reason and there is no risk from mad cow disease. However, the effects of different sizes of fish collagen hydrolysate on cartilage and chondrocyte metabolism have not been well studied yet. This study examined the effect of different sizes of fish collagen hydrolysate on cartilage metabolism...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28822069/characterization-of-ofloxacin-interaction-with-mutated-a91v-quinolone-resistance-determining-region-of-dna-gyrase-in-mycobacterium-leprae-through-computational-simulation
#53
J Nisha, V Shanthi
Mycobacterium leprae, the causal agent of leprosy is non-cultivable in vitro. Thus, the assessment of antibiotic activity against Mycobacterium leprae depends primarily upon the time-consuming mouse footpad system. The GyrA protein of Mycobacterium leprae is the target of the antimycobacterial drug, Ofloxacin. In recent times, the GyrA mutation (A91V) has been found to be resistant to Ofloxacin. This phenomenon has necessitated the development of new, long-acting antimycobacterial compounds. The underlying mechanism of drug resistance is not completely known...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28770488/identification-and-characterization-of-functional-single-nucleotide-polymorphisms-snps-in-axin-1-gene-a-molecular-dynamics-approach
#54
Imran Khan, Irfan A Ansari, Pratichi Singh, J Febin Prabhu Dass, Fahad Khan
Wnt signaling pathway has been reported to play crucial role in intestinal crypt formation and deregulation of this pathway is responsible for colorectal cancer initiation and progression. Axin 1, a scaffold protein, play pivotal role in the regulation of Wnt/β-catenin signaling pathway and has been found to be mutated in several cancers; primarily in colon cancer. Considering its crucial role, a structural and functional analysis of missense mutations in Axin 1 gene was performed in this study. Initially, one hundred non-synonymous single nucleotide polymorphisms in the coding regions of Axin 1 gene were selected for in silico analysis...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28756483/identification-of-structure-stabilizing-interactions-in-enzymes-a-novel-mechanism-to-impact-enzyme-activity
#55
Marisol Serrano, Veronica Gonzalez, Supriyo Ray, Maria D Chavez, Mahesh Narayan
Cruzain, a cysteine protease in the cathepsin family, is pivotal to the life-cycle of Trypanosoma cruzi, the etiological agent in Chagas disease. Current inhibitors of cruzain suffer from drawbacks involving gastrointestinal and neurological side effects and as a result have spurred the search for alternative anti-trypanocidals. Through sequence alignment studies and intra-residue interaction analysis of the pro-protein of cruzain (pro-cruzain), we have identified a host of non-active site residues that are conserved among the cathepsins...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28726180/epr-characterization-of-dinitrosyl-iron-complexes-with-thiol-containing-ligands-as-an-approach-to-their-identification-in-biological-objects-an-overview
#56
REVIEW
Anatoly F Vanin
The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the paramagnetic (electron paramagnetic resonance-active) mononuclear and diamagnetic (electron paramagnetic resonance-silent) binuclear forms and control the activity of nitrogen monoxide, one of the most universal regulators of metabolic processes in the organism...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28726179/methionine-and-or-methionine-sulfoxide-alter-ectoenzymes-activities-in-lymphocytes-and-inflammatory-parameters-in-serum-from-young-rats-acute-and-chronic-effects
#57
Mayara Sandrielly Pereira Soares, Marcelo Zanusso Costa, Tatiane Morgana da Silva, Marta Gazal, Carlus Augustu Tavares do Couto, Gabriela Nogueira Debom, Rodrigo Rodrigues, Juliana Hofstätter Azambuja, Emerson André Casali, Cesar Eduardo Jacintho Moritz, Marta Frescura Duarte, Elizandra Braganhol, Francieli Moro Stefanello, Roselia Maria Spanevello
In this study we investigated the effect of acute and chronic treatment with Met and/or methionine sulfoxide (MetO) on ectonucleotidases and cholinesterases activities from lymphocytes and purine derivatives compounds, C-protein reactive, interleukin-10, interleukin-6, and tumor necrosis factor-α levels in serum of young rats. Adenosine triphosphate hydrolysis was decreased in lymphocytes 1 h after treatment by MetO and Met + MetO. However, adenosine triphosphate and adenosine diphosphate hydrolysis in lymphocytes was increased in the groups MetO and Met + MetO and adenosine deaminase activity was increased in MetO 3 h after the treatment...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28646413/integrated-in-silico-in-vitro-identification-and-characterization-of-the-sh3-mediated-interaction-between-human-pttg-and-its-cognate-partners-in-medulloblastoma
#58
Jiangang Liu, Dapeng Wang, Yanyan Li, Hui Yao, Nan Zhang, Xuewen Zhang, Fangping Zhong, Yulun Huang
The human pituitary tumor-transforming gene is an oncogenic protein which serves as a central hub in the cellular signaling network of medulloblastoma. The protein contains two vicinal PxxP motifs at its C terminus that are potential binding sites of peptide-recognition SH3 domains. Here, a synthetic protocol that integrated in silico analysis and in vitro assay was described to identify the SH3-binding partners of pituitary tumor-transforming gene in the gene expression profile of medulloblastoma. In the procedure, a variety of structurally diverse, non-redundant SH3 domains with high gene expression in medulloblastoma were compiled, and their three-dimensional structures were either manually retrieved from the protein data bank database or computationally modeled through bioinformatics technique...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28623516/erratum-to-a-simple-analytical-approximation-to-an-inhomogeneously-broadened-dispersion-spectrum-application-to-absorption-dispersion-admixtures
#59
Barney L Bales
No abstract text is available yet for this article.
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/28577243/review-on-the-biological-mechanisms-associated-with-depo-provera-and-hiv-1-risk-acquisition-in-women
#60
REVIEW
Funanani Takalani, Ndumiso N Mhlongo, Suri Moonsamy, Mahmoud E S Soliman
Women constitute more than 50% out of millions of individuals infected with HIV-1, the major causative agent of acquired immune deficiency syndrome. About 40% of HIV-1 infections have been reported to initiate in the female reproductive tract. However, the mechanisms through which these infections are spread are poorly understood; hence, there is now a major concern in women who use long acting injectable hormonal contraceptives, particularly Depo-Provera and an increase of HIV-1 risk acquisition. Based on literature, Depo-Provera has an affinity for both the glucocorticoid receptor and the progesterone receptor in the female reproductive tract...
June 2018: Cell Biochemistry and Biophysics
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