journal
https://read.qxmd.com/read/38214811/dads-inhibits-the-proliferation-of-mdcc-msb-1-cells-by-inducing-autophagy-via-the-mek-erk-signalling-pathway
#41
LETTER
Yanying Pan, Jianying Liu, Dongliang Wang, Pengfei Zhou, Tao Chen, Yulong Tang, Chunxiao Ji
Diallyl disulfide (DADS) is effective at suppressing tumour cell growth and proliferation. This study verified the morphology and growth activity of MDCC-MSB-1 cells by using an MTT assay to detect the effect of DADS on the proliferation of MDCC-MSB-1 cells and a CCK8 assay to detect the effect of DADS on the viability and proliferation of MDCC-MSB-1 cells. We found that the viability and proliferation of MDCC-MSB-1 cells decreased with increasing DADS concentrations. MDC staining and Western blotting were used to analyse autophagy, the associated protein LC3 and the MEK/ERK pathway proteins MEK and ERK and to investigate changes in cellular autophagy based on cell morphology and molecular biology...
January 12, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38198024/berberine-and-rnai-targeting-telomerase-reverse-transcriptase-tert-and-or-telomerase-rna-component-terc-caused-oxidation-in-colorectal-cancer-cell-line-hct-116-an-integrative-approach-using-molecular-and-metabolomic-studies
#42
JOURNAL ARTICLE
Muhammad Azizan Samad, Mohd Zuwairi Saiman, Nazia Abdul Majid, Saiful Anuar Karsani, Jamilah Syafawati Yaacob
Colorectal cancer (CRC) is the most common cancer in both men and women and is associated with increased telomerase levels and activity. The potential downstream effects of TERT and/or TERC downregulation by berberine (a telomerase inhibitor) or RNA interference (RNAi) on various target RNAs, proteins, relative telomerase activity (RTA), relative telomere length (RTL), hydrogen peroxide concentration [H2 O2 ], percentage of cell cycle distribution, cell size and granularity as well as cellular metabolites were explored in HCT 116 cell line...
January 10, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38196050/glycolytic-enzymes-in-non-glycolytic-web-functional-analysis-of-the-key-players
#43
REVIEW
Avirup Malla, Suvroma Gupta, Runa Sur
To survive in the tumour microenvironment, cancer cells undergo rapid metabolic reprograming and adaptability. One of the key characteristics of cancer is increased glycolytic selectivity and decreased oxidative phosphorylation (OXPHOS). Apart from ATP synthesis, glycolysis is also responsible for NADH regeneration and macromolecular biosynthesis, such as amino acid biosynthesis and nucleotide biosynthesis. This allows cancer cells to survive and proliferate even in low-nutrient and oxygen conditions, making glycolytic enzymes a promising target for various anti-cancer agents...
January 9, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38183602/chemotherapeutic-and-antiproliferative-effect-of-purified-protein-from-marine-catfish-tachysurus-dussumieri-on-human-colon-cancer-cell-line
#44
JOURNAL ARTICLE
Kavitha Raja, Parashuram Kallem, Arulvasu Chinnasamy
The present study aimed to investigate the purified protein from the epidermal mucus of marine catfish Tachysurus dussumieri on the human colon cancer cell line. The bioactive protein was purified with the Anion exchange chromatography and the collected fractions were then tested to assess cell viability in HT 29 cells through the MTT assay. The most responding active purified protein fraction (PPF III) was characterized with the MALDI-TOF/MS it shared a similar homology and sequence with 90% of antimicrobial peptides from external secretions of amphibians...
January 6, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38183601/curcumin-and-ferroptosis-a-promising-target-for-disease-prevention-and-treatment
#45
REVIEW
Zahra Foroutan, Alexandra E Butler, Gokhan Zengin, Amirhossein Sahebkar
Ferroptosis is a recently identified form of cell death characterized by iron accumulation and lipid peroxidation. Unlike apoptosis, necrosis, and autophagy, ferroptosis operates through a distinct molecular pathway. Curcumin, derived from turmeric rhizomes, is a natural compound with diverse therapeutic benefits, including neuroprotective, anti-metabolic syndrome, anti-inflammatory, and anti-cancer properties. Growing evidence suggests that curcumin possesses both pro-oxidant and antioxidant properties, which can vary depending on the cell type...
January 6, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38182861/multi-omics-analyses-reveal-function-of-apolipoprotein-e-in-alternative-splicing-and-tumor-immune-microenvironment-in-kidney-renal-clear-cell-carcinoma-via-pan-cancer-analysis
#46
LETTER
Xin Leng, Jianhu Liu, Anqi Jin, Hongfang Zheng, Jiulong Wu, Longfei Zhong, Qiaoxin Li, Dongfeng Li
Apolipoprotein E (APOE) regulates lipid metabolism, associated with the development of various cancers. However, its precise prognostic significance and functions in alternative splicing and the tumor immune microenvironment remain unclear. In this study, we extracted APOE expression in pan-cancer from TCGA and analyzed mRNA transcriptome, cell lines, and protein levels. Furthermore, we analyzed the alternative splicing expression of the APOE gene transcript with prognostic profiles using the OncoSplicing database...
January 6, 2024: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38133792/single-cell-sequencing-technology-and-its-application-in-the-study-of-central-nervous-system-diseases
#47
REVIEW
Yang Ding, Yu-Yuan Peng, Sen Li, Can Tang, Jie Gao, Hai-Yan Wang, Zai-Yun Long, Xiu-Min Lu, Yong-Tang Wang
The mammalian central nervous system consists of a large number of cells, which contain not only different types of neurons, but also a large number of glial cells, such as astrocytes, oligodendrocytes, and microglia. These cells are capable of performing highly refined electrophysiological activities and providing the brain with functions such as nutritional support, information transmission and pathogen defense. The diversity of cell types and individual differences between cells have brought inspiration to the study of the mechanism of central nervous system diseases...
December 22, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38133791/tubulin-regulates-plasma-membrane-ca-2-atpase-activity-in-a-lipid-environment-dependent-manner
#48
REVIEW
Noelia E Monesterolo, Verónica S Santander, Alexis N Campetelli, Juan F Rivelli Antonelli, Ayelén D Nigra, Melisa M Balach, Tamara Muhlberger, Gabriela Previtali, César H Casale
Ca2+ plays a crucial role in cell signaling, cytosolic Ca2+ can change up to 10,000-fold in concentration due to the action of Ca2+ -ATPases, including PMCA, SERCA and SCR. The regulation and balance of these enzymes are essential to maintain cytosolic Ca2+ homeostasis. Our laboratory has discovered a novel PMCA regulatory system, involving acetylated tubulin alone or in combination with membrane lipids. This regulation controls cytosolic Ca2+ levels and influences cellular properties such as erythrocyte rheology...
December 22, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38129709/hypoxia-potentiated-lung-cancer-cell-migration-and-invasion-by-up-regulating-hif1%C3%AE-jak2-stat3-axis-and-activating-mmp13-transcription
#49
JOURNAL ARTICLE
Xiaoju Cheng, Damin Liang, Xiaoqian Li, Chengmin Deng, Meng Ye, Jiao Yang, Yurui Liu, Kaifeng Wu, Jie Wu, Peng Tian
Excessive aggressive migration and invasion are important factors that increase the mortality of cancer patients. Matrix metalloproteinase 13 (MMP13) expression is positively correlated with lung cancer malignancy. However, the mechanism underlying an elevated MMP13 expression is not clearly defined. In this study, we demonstrated that hypoxia induced by CoCl2 enhanced the expression of HIF1α, JAK2, STAT3 and MMP13 in A549 cells. A positive correlation between HIF1α and MMP13 expression was observed in lung adenocarcinoma patients...
December 21, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38082173/%C3%AE-carotene-inhibits-mapks-signaling-pathways-on-rat-colonic-epithelial-cells-to-attenuate-tnf-%C3%AE-induced-intestinal-inflammation-and-injury
#50
JOURNAL ARTICLE
Yang Song, Lingyu Zhu, Xin Zheng
This experiment successfully isolated the rat colonic epithelial cells and established a TNF-α-induced intestinal inflammation model. Western Blot was used to detect the related protein expression levels of the MAPKs signaling pathway. QPCR technology was used to detect the expression of aquaporins, intestinal mucosal repair factor, and inflammatory factors. The results show that 25 μM β-carotene pretreatment at 24 h can inhibit MAPKs signaling pathway activated by TNF-α, change the relative mRNA expression of inflammatory cytokines, intestinal mucosal repair factors, and aquaporins, and the phosphorylated protein expression of p38, ERK, and NF-κB were attenuated to reduce inflammatory damage...
December 11, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38064100/cardioprotective-effect-of-2-ethyl-3-hydroxy-6-methylpyridinium-2-nitroxysuccinate-against-adrenaline-hydrocortisone-induced-myocardial-ischemia-in-mice-modulation-of-free-radical-processes-in-biomembranes-and-monoamine-oxidase-a-activity
#51
JOURNAL ARTICLE
Irina I Faingold, Anastasia V Smolina, Yulia V Soldatova, Darya A Poletaeva, Anastasia A Balakina, Tatyana E Sashenkova, Uguljan Yu Allayarova, Tatyana R Prikhodchenko, Svetlana V Blokhina, Lyudmila A Makartseva, David A Areshidze, Vladislav N Varfolomeev, Denis V Mishchenko, Raisa A Kotelnikova
Oxidative stress (OS) plays a key role in the development of cardiovascular diseases (CVD) in three major ways: reactive oxygen species (ROS)-induced reduction of nitric oxide (NO) bioavailability, ROS-induced inflammation and ROS-induced mitochondrial dysfunction. Oxidation of lipid molecules under the action of ROS leads to damage to membrane structures, changes the functioning of membrane-bound enzymes, and impairs membrane permeability and stability. An increase in OS results in the occurrence of endothelial dysfunction and drug tolerance, side effects, requiring discontinuation of drugs...
December 8, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38048024/understanding-cftr-functionality-a-comprehensive-review-of-tests-and-modulator-therapy-in-cystic-fibrosis
#52
REVIEW
Shorya Thakur, Ankita, Shubham Dash, Rupali Verma, Charanjit Kaur, Rajesh Kumar, Avijit Mazumder, Gurvinder Singh
Cystic fibrosis is a genetic disorder inherited in an autosomal recessive manner. It is caused by a mutation in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene on chromosome 7, which leads to abnormal regulation of chloride and bicarbonate ions in cells that line organs like the lungs and pancreas. The CFTR protein plays a crucial role in regulating chloride ion flow, and its absence or malfunction causes the production of thick mucus that affects several organs. There are more than 2000 identified mutations that are classified into seven categories based on their dysfunction mechanisms...
December 4, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38040891/evaluation-of-the-moonlighting-histone-h3-specific-protease-h3ase-activity-and-the-dehydrogenase-activity-of-glutamate-dehydrogenase-gdh
#53
JOURNAL ARTICLE
Jogeswar Satchidananda Purohit, Madhulika Singh, Yashankita Raghuvanshi, Saima Syeda, Madan M Chaturvedi
The N-terminus of Histone H3 is proteolytically processed in aged chicken liver. A histone H3 N-terminus specific endopeptidase (named H3ase) has been purified from the nuclear extract of aged chicken liver. By sequencing and a series of biochemical methods including the demonstration of H3ase activity in bacterially expressed GDH, it was established that the H3ase activity was a moonlighting protease activity of glutamate dehydrogenase (GDH). However, the active site for the H3ase in the GDH remains elusive...
December 1, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/38010601/correction-thumpd3-as1-facilitates-cell-growth-and-aggressiveness-by-the-mir-218-5p-skap1-axis-in-colorectal-cancer
#54
Yuwei Pu, Jinrong Wei, Yong Wu, Kui Zhao, Yongyou Wu, Shu Wu, Xiaodong Yang, Chungen Xing
No abstract text is available yet for this article.
November 27, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37995086/cholesterol-hydroperoxide-co-trafficking-in-testosterone-generating-leydig-cells-gpx4-inhibition-of-cytotoxic-and-anti-steroidogenic-effects
#55
JOURNAL ARTICLE
Pawel Pabisz, Jerzy Bazak, Michal Sabat, Albert W Girotti, Witold Korytowski
Trafficking of intracellular cholesterol (Ch) to and into mitochondria of steroidogenic cells is required for steroid hormone biosynthesis. This trafficking is typically mediated by one or more proteins of the steroidogenic acute regulatory (StAR) family. Our previous studies revealed that 7-OOH, a redox-active cholesterol hydroperoxide, could be co-trafficked with Ch to/into mitochondria of MA-10 Leydig cells, thereby inducing membrane lipid peroxidation (LPO) which impaired progesterone biosynthesis. These negative effects of 7-OOH were inhibited by endogenous selenoperoxidase GPx4, indicating that this enzyme could protect against 7-OOH-induced oxidative damage/dysfunction...
November 23, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37978103/new-perspective-on-the-interaction-behavior-between-riboflavin-and-%C3%AE-lactoglobulin-%C3%AE-casein-complex-by-biophysical-techniques
#56
JOURNAL ARTICLE
Farzaneh Samandar, Sara Malek-Mohammadi, Zahra Aram, Farangis Rastin, Shakiba Tolou-Shikhzadeh-Yazdi, Zeinab Amiri-Tehranizadeh, Mohammad Reza Saberi, Jamshidkhan Chamani
Riboflavin (RF) is a vitamin that only exists in plants and microorganisms and must be procured externally by humans. On the other hand, there are two major allergic factors in cow's milk, including β-lactoglobulin (βLG) and β-casein (βCN), while their allergic properties can be eliminated by binding to micronutrients. In this regard, we examined the binding process of RF to βLG and βCN in the binary and ternary systems by different spectroscopies such as zeta potential, electric conductivity, and molecular modeling...
November 17, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37966623/peculiarities-of-glucose-molecules-destruction-under-irradiation-at-the-m-30-microtron-12-5%C3%A2-mev-mass-spectrometric-studies
#57
JOURNAL ARTICLE
V T Maslyuk, A N Zavilopulo, N I Svatiuk, Y A Bandurin
The method of mass spectrometric studies was used to study the fragmentation of glucose in the gas phase upon collision with low-energy electrons (20-70 eV) before and after irradiation at the M-30 microtron (12.5 MeV) with doses of 14 and 164 kGy. The dose dependence of the transformation of glucose mass spectra was established. The results indicate the dominance in mass spectra of symmetric fission channels of the molecule itself and its fragments formed under the action of M-30 microtron radiation...
November 15, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37962751/an-intriguing-structural-modification-in-neutrophil-migration-across-blood-vessels-to-inflammatory-sites-progress-in-the-core-mechanisms
#58
REVIEW
Zexu Wang, Yufang Guo, Yulei Zhang, Liangquan Wu, Li Wang, Qiuqi Lin, Bing Wan
The role and function of neutrophils are well known, but we still have incomplete understanding of the mechanisms by which neutrophils migrate from blood vessels to inflammatory sites. Neutrophil migration is a complex process that involves several distinct steps. To resist the blood flow and maintain their rolling, neutrophils employ tether and sling formation. They also polarize and form pseudopods and uropods, guided by hierarchical chemotactic agents that enable precise directional movement. Meanwhile, chemotactic agents secreted by neutrophils, such as CXCL1, CXCL8, LTB4, and C5a, can recruit more neutrophils and amplify their response...
November 14, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37870699/immune-features-of-tumor-microenvironment-a-genetic-spotlight
#59
REVIEW
Milad Asadi, Habib Zarredar, Venus Zafari, Zahra Soleimani, Hossein Saeedi, Ayse Caner, Dariush Shanehbandi
A tumor represents a highly intricate tissue entity, characterized by an exceptionally complex microenvironment that starkly contrasts with the typical physiological surroundings of healthy tissues. Within this tumor microenvironment (TME), every component and factor assume paramount importance in the progression of malignancy and exerts a pivotal influence on a patient's clinical outcome. One of the remarkable aspects of the TME is its remarkable heterogeneity, not only across different types of cancers but even within the same histological category of tumors...
October 23, 2023: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/37853249/role-of-toll-like-receptor-in-the-pathogenesis-of-oral-cancer
#60
REVIEW
Ananya Bhardwaj, Divya Prasad, Sayali Mukherjee
Toll-like receptors are important molecules of innate immunity. They are known as pattern recognition receptors. They recognise certain molecules known as pathogen-associated molecular pattern on a pathogen and release chemicals that causes inflammation. Toll-like receptors (TLR) help in the removal of the infected cell and thus stop the spread of infection and are being studied for their association with cancer. Oral carcinoma has emerged as a major problem of our country today; it is found ranks first in men and third in women...
October 19, 2023: Cell Biochemistry and Biophysics
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