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Cell Biochemistry and Biophysics | Page 2

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https://read.qxmd.com/read/30191452/the-iron-state-in-spleen-and-liver-tissues-from-patients-with-hematological-malignancies-studied-using-magnetization-measurements-and-m%C3%A3-ssbauer-spectroscopy
#21
I V Alenkina, A V Vinogradov, I Felner, T S Konstantinova, E Kuzmann, V A Semionkin, M I Oshtrakh
In this overview, we present the results of the study of spleen and liver tissues taken from healthy donors in comparison with those from patients with (i) non-Hodgkin B-cell lymphomas, namely, mantle cell lymphoma and marginal zone B-cell lymphoma, (ii) acute myeloid leukemia, and (iii) primary myelofibrosis. The study was carried out using Mössbauer spectroscopy and magnetization measurements for the analysis of ferritin-like iron in spleen and liver tissues. Magnetization measurements demonstrated small differences in the saturation magnetic moments and revealed additional paramagnetic components...
September 7, 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/30073572/induced-mutation-proves-a-potential-target-for-tb-therapy-a-molecular-dynamics-study-on-lprg
#22
Kgothatso E Machaba, Ndumiso N Mhlongo, Mahmoud E S Soliman
Molecular dynamics (MD) simulations of wild-type and V91W mutant Mycobacterium tuberculosis-LprG (Mtb-LprG) were performed with the goal to provide a comprehensive understanding of the Mtb-LprG as a potential antimycobacterial target. A long-range MD simulations and post-MD analyzes led us to various results that plainly explained the impact of V91W mutation on Mtb-LprG. Herein, the results revealed that the wild-type is less stable compared to V91W mutant. This was further supported by root mean square fluctuation, where the V91W mutant showed a higher degree of flexibility compared to the wild-type...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/30062659/quantum-chemical-calculations-and-statistical-analysis-structural-cytotoxicity-relationships-of-some-synthesized-2-thiophen-naphtho-benzo-oxazinone-derivatives
#23
Mohammed B Alshammari, Mohammed H Geesi, El Hassane Anouar, Rashad Al-Salahi, Abdulrahman I Alharthi, Yasser Elnakady, Mohamed Marzouk
Twenty-two 2-thiophen-naphtho(benzo)oxazinone derivatives are prepared using 3-amino-2-naphthoic and 5-nitroanthranilic acids as building blocks. The target compounds (1-22) were evaluated quantitatively for their cytotoxic effects in vitro against three cancer cell lines, including the lung A549, the hepatocyte HepG2, and the breast MCF-7 carcinoma cells. Compounds 1, 12, 14, and 21 were found to exhibit remarkable cytotoxicity against the tested cancer cell lines. Compound 21 has shown the highest activity against A549 and MCF-7 (IC50 : 9...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/30022374/endocytosis-of-g-protein-coupled-receptors-and-their-ligands-is-there-a-role-in-metal-trafficking
#24
REVIEW
Katerina Christofides, Resmi Menon, Christopher E Jones
The prevalence of metal dysregulation in many neurodegenerative and neurocognitive disorders has compelled many studying such diseases to investigate the mechanisms underlying metal regulation in the central nervous system. Metal homoeostasis is often complex, with sophisticated, multilayered pathways in operation. G protein-coupled receptors are omnipresent on cell membranes and have intriguing mechanisms of endocytosis and trafficking that may be useful in metal homoeostasis. Indeed, many receptors and/or their cognate ligands are able to bind metals, and in many cases metals are considered to have neuromodulatory roles as a result of receptor binding...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29956081/p47phox-dependent-reactive-oxygen-species-stimulate-nuclear-translocation-of-the-foxo1-transcription-factor-during-metabolic-inhibition-in-cardiomyoblasts
#25
Ellis N Ter Horst, Nynke E Hahn, Dirk Geerts, René J P Musters, Walter J Paulus, Albert C van Rossum, Christof Meischl, Jan J Piek, Hans W M Niessen, Paul A J Krijnen
Reactive oxygen species (ROS) control forkhead box O (FOXO) transcription factor activity by influencing their nuclear translocation. However, knowledge of the ROS cellular source(s) involved herein remains scarce. Recently, we have shown p47phox -dependent activation of ROS-producing NADPH oxidase (NOX) at the nuclear pore in H9c2 rat cardiomyoblasts in response to ischemia. This localizes NOX perfectly to affect protein nuclear translocation, including that of transcription factors. In the current study, involvement of p47phox -dependent production of ROS in the nuclear translocation of FOXO1 was analyzed in H9c2 cells following 4 h of metabolic inhibition (MI), which mimics the effects of ischemia...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29687225/hyphenated-3d-qsar-statistical-model-drug-repurposing-analysis-for-the-identification-of-potent-neuraminidase-inhibitor
#26
K Rohini, V Shanthi
The Influenza A virus is one of the principle causes of respiratory illness in human. The surface glycoprotein of the influenza virus, neuraminidase (NA), has a vital role in the release of new viral particle and spreads infection in the respiratory tract. It has been long recognized as a valid drug target for influenza A virus infection. Oseltamivir is used as a standard drug of choice for the treatment of influenza. However, the emergence of mutants with novel mutations has increased the resistance to potent NA inhibitor...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29556953/correction-to-isolation-and-characterization-of-a-microrna-size-secretable-small-rna-in-streptococcus-sanguinis
#27
Ji-Woong Choi, Tae-Yub Kwon, Su-Hyung Hong, Heon-Jin Lee
The original version of this article unfortunately contains a mistake in the fund number under Acknowledgment section. The funding number should read as "2017R1A5A2015391 and 2015R1D1A3A01019684".
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29511990/on-the-regularities-of-the-polar-profiles-of-proteins-related-to-ebola-virus-infection-and-their-functional-domains
#28
Carlos Polanco, José Lino Samaniego Mendoza, Thomas Buhse, Vladimir N Uversky, Ingrid Paola Bañuelos Chao, Marcela Angola Bañuelos Cedano, Fernando Michel Tavera, Daniel Michel Tavera, Manuel Falconi, Abelardo Vela Ponce de León
The number of fatalities and economic losses caused by the Ebola virus infection across the planet culminated in the havoc that occurred between August and November 2014. However, little is known about the molecular protein profile of this devastating virus. This work represents a thorough bioinformatics analysis of the regularities of charge distribution (polar profiles) in two groups of proteins and their functional domains associated with Ebola virus disease: Ebola virus proteins and Human proteins interacting with Ebola virus...
September 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/30047043/conformational-changes-and-membrane-interaction-of-the-bacterial-phospholipase-exou-characterization-by-site-directed-spin-labeling
#29
Jimmy B Feix, Samantha Kohn, Maxx H Tessmer, David M Anderson, Dara W Frank
Numerous pathogenic bacteria produce proteins evolved to facilitate their survival and dissemination by modifying the host environment. These proteins, termed effectors, often play a significant role in determining the virulence of the infection. Consequently, bacterial effectors constitute an important class of targets for the development of novel antibiotics. ExoU is a potent phospholipase effector produced by the opportunistic pathogen Pseudomonas aeruginosa. Previous studies have established that the phospholipase activity of ExoU requires non-covalent interaction with ubiquitin, however the molecular details of the mechanism of activation and the manner in which ExoU associates with a target lipid bilayer are not understood...
July 25, 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/30003432/transforming-growth-factor-beta-tfg-%C3%AE-concentration-isoforms-are-diminished-in-acute-coronary-syndrome
#30
Jorge Ramón Padilla-Gutiérrez, Emmanuel Valdés-Alvarado, Saraí Citlalic Rodríguez-Reyes, Juan Arellano-Martin, Héctor Enrique Flores-Salinas, José Francisco Muñoz Valle, Yeminia Valle
Acute coronary syndrome (ACS) is the leading cause of death in elderly patients worldwide. Due its participation in apoptosis, fibrosis, and angiogenesis, transforming growth factor-β (TGF-β) isoforms had been categorized as risk factors for cardiovascular diseases. However, due their contradictory activities, a cardioprotective role has been suggested. The aim was to measure the plasma levels of TGF-β1, 2, and 3 proteins in patients with ACS. This was a case-control study including 225 subjects. The three activated isoforms were measured in serum using the Bio-Plex Pro TGF-β assay by means of magnetic beads; the fluorescence intensity of reporter signal was read in a Bio-Plex Magpix instrument...
July 12, 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29943362/uniform-field-resonators-for-epr-spectroscopy-a-review
#31
James S Hyde, Jason W Sidabras, Richard R Mett
Cavity resonators are often used for electron paramagnetic resonance (EPR). Rectangular TE102 and cylindrical TE011 are common modes at X-band even though the field varies cosinusoidally along the Z-axis. The authors found a way to create a uniform field (UF) in these modes. A length of waveguide at cut-off was introduced for the sample region, and tailored end sections were developed that supported the microwave resonant mode. This work is reviewed here. The radio frequency (RF) magnetic field in loop-gap resonators (LGR) at X-band is uniform along the Z-axis of the sample, which is a benefit of LGR technology...
June 25, 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29926429/targeting-outer-membrane-protein-component-adec-for-the-discovery-of-efflux-pump-inhibitor-against-adeabc-efflux-pump-of-multidrug-resistant-acinetobacter-baumannii
#32
Privita Verma, Vishvanath Tiwari
The structure and functioning of multidrug efflux systems provide us with a better understanding of the transport of various antibiotics, thus giving a path for the discovery of effective compounds for combating the multidrug resistance in Acinetobacter baumannii. In the present study, a number of computational techniques have been used to search for an inhibitor for the RND efflux pump, AdeABC, of A. baumannii targeting specifically its outermost component, i.e., AdeC. We have prepared the three-dimensional structure for AdeC using MODELLER v9...
June 20, 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29572698/2018-letter-from-the-editor-in-chief
#33
EDITORIAL
Lawrence Berliner
No abstract text is available yet for this article.
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29380245/screening-of-potential-inhibitor-against-coat-protein-of-apple-chlorotic-leaf-spot-virus
#34
Rituraj Purohit, Sachin Kumar, Vipin Hallan
In this study, we analyzed Coat protein (CP) of Apple chlorotic leaf spot virus (ACLSV), an important latent virus on Apple. Incidence of the virus is upto 60% in various apple cultivars, affecting yield losses of the order of 10-40% (depending upon the cultivar). CP plays an important role as the sole building block of the viral capsid. Homology approach was used to model 193 amino acid sequence of the coat protein. We used various servers such as ConSurf, TargetS, OSML, COACH, COFACTOR for the prediction of active site residues in coat protein...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29322360/highly-conserved-arg-residue-of-erfnin-motif-of-pro-domain-is-important-for-ph-induced-zymogen-activation-process-in-cysteine-cathepsins-k-and-l
#35
Pulakesh Aich, Sampa Biswas
Pro-domain of a cysteine cathepsin contains a highly conserved Ex2 Rx2 Fx2 Nx3 Ix3 N (ERFNIN) motif. The zymogen structure of cathepsins revealed that the Arg(R) residue of the motif is a central residue of a salt-bridge/H-bond network, stabilizing the scaffold of the pro-domain. Importance of the arginine is also demonstrated in studies where a single mutation (Arg → Trp) in human lysosomal cathepsin K (hCTSK) is linked to a bone-related genetic disorder "Pycnodysostosis". In the present study, we have characterized in vitro Arg → Trp mutant of hCTSK and the same mutant of hCTSL...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29313175/a-biomolecular-network-driven-proteinic-interaction-in-hcv-clearance
#36
Pratichi Singh, Febin Prabhu Dass J
Hepatitis C virus infection causes chronic liver disease that leads to cancer-related mortality. Presently around 30% of the HCV (infected) affected population get rid of the infection through spontaneous disease clearance. This phenomenon is conducted by a set of reported immune candidate genes. Hence, this study focuses only on these immune-response related genes with aid of network approach, where the idea is to disseminate the network for better understanding of key functional genes and their transcription control activity...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29134494/expression-of-telomere-associated-proteins-is-interdependent-to-stabilize-native-telomere-structure-and-telomere-dysfunction-by-g-quadruplex-ligand-causes-terra-upregulation
#37
Ratan Sadhukhan, Priyanka Chowdhury, Sourav Ghosh, Utpal Ghosh
Telomere DNA can form specialized nucleoprotein structure with telomere-associated proteins to hide free DNA ends or G-quadruplex structures under certain conditions especially in presence of G-quadruplex ligand. Telomere DNA is transcribed to form non-coding telomere repeat-containing RNA (TERRA) whose biogenesis and function is poorly understood. Our aim was to find the role of telomere-associated proteins and telomere structures in TERRA transcription. We silenced four [two shelterin (TRF1, TRF2) and two non-shelterin (PARP-1, SLX4)] telomere-associated genes using siRNA and verified depletion in protein level...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29116599/the-class-a-gpcr-dopamine-d2-receptor-forms-transient-dimers-stabilized-by-agonists-detection-by-single-molecule-tracking
#38
Rinshi S Kasai, Shuichi V Ito, Ryo M Awane, Takahiro K Fujiwara, Akihiro Kusumi
Whether class-A G-protein coupled receptors (GPCRs) exist and work as monomers or dimers has drawn extensive attention. A class-A GPCR dopamine D2 receptor (D2R) is involved in many physiological and pathological processes and diseases, indicating its critical role in proper functioning of neuronal circuits. In particular, D2R homodimers might play key roles in schizophrenia development and amphetamine-induced psychosis. Here, using single-molecule imaging, we directly tracked single D2R molecules in the plasma membrane at a physiological temperature of 37 °C, and unequivocally determined that D2R forms transient dimers with a lifetime of 68 ms in its resting state...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29090414/liver-genes-expression-induced-by-tamoxifen-loaded-solid-lipid-nanoparticles-in-wistar-female-rats
#39
Naser Zanganeh, Nasrin Ziamajidi, Iraj Khodadadi, Massoud Saidijam, Roghayeh Abbasalipourkabir
The objective of this study was to investigate the effect of free tamoxifen and tamoxifen-loaded solid lipid nanoparticles (SLN) on cytochrome P450 (CYP3A2) and flavin-containing monooxygenase1 (FMO1) genes expression in the liver of female Wistar rats. Thirty female Wistar rats aged 7-8 weeks, were divided into six groups of six rats each. The first, second, third, and fourth groups were ovariectomized and received tamoxifen (2  mg/kg of body weight dissolved in 1  ml olive oil), tamoxifen-loaded SLN (2  mg/kg of body weight dispersed in 1  ml olive oil), SLN (10  mg/kg of body weight dispersed in 1  ml olive oil), and 1  ml olive oil, respectively...
June 2018: Cell Biochemistry and Biophysics
https://read.qxmd.com/read/29067585/intrinsic-response-towards-physiologic-stiffness-is-cell-type-dependent
#40
Michael Reimer, Silviya Petrova Zustiak, Saahil Sheth, Joseph Martin Schober
In the continuous search for better tissue engineering scaffolds it has become increasingly clear that the substrate properties dramatically affect cell responses. Here we compared cells from a physiologically stiff tissue, melanoma, to cells isolated from a physiologically soft tissue, brain. We measured the cell line responses to laminin immobilized onto glass or polyacrylamide hydrogels tuned to have a Young's modulus ranging from 1 to 390 kPa. Single cells were analyzed for spreading area, shape, total actin content, actin-based morphological features and modification of immobilized laminin...
June 2018: Cell Biochemistry and Biophysics
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