journal
https://read.qxmd.com/read/38871815/optimal-design-of-synthetic-circular-rnas
#21
REVIEW
Seo-Won Choi, Jin-Wu Nam
Circular RNAs are an unusual class of single-stranded RNAs whose ends are covalently linked via back-splicing. Due to their versatility, the need to express circular RNAs in vivo and in vitro has increased. Efforts have been made to efficiently and precisely synthesize circular RNAs. However, a review on the optimization of the processes of circular RNA design, synthesis, and delivery is lacking. Our review highlights the multifaceted aspects considered when producing optimal circular RNAs and summarizes the available options for each step of exogenous circular RNA design and synthesis, including circularization strategies...
June 14, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38871814/inverted-alu-repeats-friends-or-foes-in-the-human-transcriptome
#22
REVIEW
Keonyong Lee, Jayoung Ku, Doyeong Ku, Yoosik Kim
Alu elements are highly abundant primate-specific short interspersed nuclear elements that account for ~10% of the human genome. Due to their preferential location in gene-rich regions, especially in introns and 3' UTRs, Alu elements can exert regulatory effects on the expression of both host and neighboring genes. When two Alu elements with inverse orientations are positioned in close proximity, their transcription results in the generation of distinct double-stranded RNAs (dsRNAs), known as inverted Alu repeats (IRAlus)...
June 14, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38871813/regulatory-rna-from-molecular-insights-to-therapeutic-frontiers
#23
EDITORIAL
TaeSoo Kim, Tae-Kyung Kim
No abstract text is available yet for this article.
June 14, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38866910/the-identification-of-effective-tumor-suppressing-neoantigens-using-a-tumor-reactive-til-tcr-pmhc-ternary-complex
#24
JOURNAL ARTICLE
Sang Hoon Kim, Bo Ryeong Lee, Sung-Min Kim, Sungsik Kim, Min-Seok Kim, Jaehyun Kim, Inkyu Lee, Hee-Soo Kim, Gi-Hoon Nam, In-San Kim, Kyuyoung Song, Yoonjoo Choi, Dong-Sup Lee, Woong-Yang Park
Neoantigens are ideal targets for cancer immunotherapy because they are expressed de novo in tumor tissue but not in healthy tissue and are therefore recognized as foreign by the immune system. Advances in next-generation sequencing and bioinformatics technologies have enabled the quick identification and prediction of tumor-specific neoantigens; however, only a small fraction of predicted neoantigens are immunogenic. To improve the predictability of immunogenic neoantigens, we developed the in silico neoantigen prediction workflows VACINUSpMHC and VACINUSTCR: VACINUSpMHC incorporates physical binding between peptides and MHCs (pMHCs), and VACINUSTCR integrates T cell reactivity to the pMHC complex through deep learning-based pairing with T cell receptors (TCRs) of putative tumor-reactive CD8 tumor-infiltrating lymphocytes (TILs)...
June 12, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38858450/author-correction-regulation-of-autophagy-by-perilysosomal-calcium-a-new-player-in-%C3%AE-cell-lipotoxicity
#25
Ha Thu Nguyen, Andreas Wiederkehr, Claes B Wollheim, Kyu-Sang Park
No abstract text is available yet for this article.
June 11, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825648/dissecting-the-muc5ac-anxa2-signaling-axis-implications-for-brain-metastasis-in-lung-adenocarcinoma
#26
JOURNAL ARTICLE
Sanjib Chaudhary, Jawed Akhtar Siddiqui, Muthamil Iniyan Appadurai, Shailendra Kumar Maurya, Swathi P Murakonda, Elizabeth Blowers, Ben J Swanson, Mohd Wasim Nasser, Surinder K Batra, Imayavaramban Lakshmanan, Apar Kishor Ganti
Non-small cell lung carcinoma (NSCLC) exhibits a heightened propensity for brain metastasis, posing a significant clinical challenge. Mucin 5ac (MUC5AC) plays a pivotal role in the development of lung adenocarcinoma (LUAD); however, its role in causing brain metastases remains unknown. In this study, we aimed to investigate the contribution of MUC5AC to brain metastasis in patients with LUAD utilizing various brain metastasis models. Our findings revealed a substantial increase in the MUC5AC level in LUAD brain metastases (LUAD-BrM) samples and brain-tropic cell lines compared to primary samples or parental control cell lines...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825647/neuron-type-specific-optogenetic-stimulation-for-differential-stroke-recovery-in-chronic-capsular-infarct
#27
JOURNAL ARTICLE
Ra Gyung Kim, Jongwook Cho, Ji-Young Park, Young Ro Kim, Min-Cheol Lee, Hyoung-Ihl Kim
Cortical neuromodulation (CNM) is widely used to promote recovery after stroke. Despite the beneficial results of CNM, the roles played by different neuron types in the effects of current CNM techniques are unable to be differentiated. Our aim was to use selective optogenetic cortical stimulation to explore how different subpopulations of neuronal cells contribute to poststroke recovery. We transduced the sensory-parietal cortex (SPC) of rats with CamKII-ChR2 (pyramidal neurons), PV-ChR2 (parvalbumin-expressing inhibitory neurons), or hSyn-ChR2 (pan-neuronal population) before inducing photothrombotic capsular infarct lesions...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825646/transcriptional-regulation-of-tfh-dynamics-and-the-formation-of-immunological-synapses
#28
REVIEW
Ye-Ji Kim, Jinyong Choi, Youn Soo Choi
Inside germinal centers (GCs), antigen-specific B cells rely on precise interactions with immune cells and strategic localization between the dark and light zones to clonally expand, undergo affinity maturation, and differentiate into long-lived plasma cells or memory B cells. Follicular helper T (Tfh) cells, the key gatekeepers of GC-dependent humoral immunity, exhibit remarkable dynamic positioning within secondary lymphoid tissues and rely on intercellular interactions with antigen-presenting cells (APCs) during their differentiation and execution of B-cell-facilitating functions within GCs...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825644/advances-and-challenges-in-modeling-inherited-peripheral-neuropathies-using-ipscs
#29
REVIEW
Jonas Van Lent, Robert Prior, Gonzalo Pérez Siles, Anthony N Cutrupi, Marina L Kennerson, Tim Vangansewinkel, Esther Wolfs, Bipasha Mukherjee-Clavin, Zachary Nevin, Luke Judge, Bruce Conklin, Henna Tyynismaa, Alex J Clark, David L Bennett, Ludo Van Den Bosch, Mario Saporta, Vincent Timmerman
Inherited peripheral neuropathies (IPNs) are a group of diseases associated with mutations in various genes with fundamental roles in the development and function of peripheral nerves. Over the past 10 years, significant advances in identifying molecular disease mechanisms underlying axonal and myelin degeneration, acquired from cellular biology studies and transgenic fly and rodent models, have facilitated the development of promising treatment strategies. However, no clinical treatment has emerged to date...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825643/the-m-6-a-writer-rbm15-drives-the-growth-of-triple-negative-breast-cancer-cells-through-the-stimulation-of-serine-and-glycine-metabolism
#30
JOURNAL ARTICLE
Su Hwan Park, Jin-Sung Ju, Hyunmin Woo, Hye Jin Yun, Su Bin Lee, Seok-Ho Kim, Balázs Győrffy, Eun-Jeong Kim, Ho Kim, Hee Dong Han, Seong-Il Eyun, Jong-Ho Lee, Yun-Yong Park
N6 -adenosine methylation (m6 A) is critical for controlling cancer cell growth and tumorigenesis. However, the function and detailed mechanism of how m6 A methyltransferases modulate m6 A levels on specific targets remain unknown. In the current study, we identified significantly elevated levels of RBM15, an m6 A writer, in basal-like breast cancer (BC) patients compared to nonbasal-like BC patients and linked this increase to worse clinical outcomes. Gene expression profiling revealed correlations between RBM15 and serine and glycine metabolic genes, including PHGDH, PSAT1, PSPH, and SHMT2...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825642/il-33-and-il-33-derived-dc-based-tumor-immunotherapy
#31
REVIEW
Myeong-Ho Kang, Yong-Soo Bae
Interleukin-33 (IL-33), a member of the IL-1 family, is a cytokine released in response to tissue damage and is recognized as an alarmin. The multifaceted roles of IL-33 in tumor progression have sparked controversy within the scientific community. However, most findings generally indicate that endogenous IL-33 has a protumor effect, while exogenous IL-33 often has an antitumor effect in most cases. This review covers the general characteristics of IL-33 and its effects on tumor growth, with detailed information on the immunological mechanisms associated with dendritic cells (DCs)...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825641/chronic-ultraviolet-irradiation-induces-memory-deficits-via-dysregulation-of-the-dopamine-pathway
#32
JOURNAL ARTICLE
Kyeong-No Yoon, Sun Yong Kim, Jungeun Ji, Yidan Cui, Qing-Ling Quan, Gunhyuk Park, Jang-Hee Oh, Ji Su Lee, Joon-Yong An, Jin Ho Chung, Yong-Seok Lee, Dong Hun Lee
The effects of ultraviolet (UV) radiation on brain function have previously been investigated; however, the specific neurotransmitter-mediated mechanisms responsible for UV radiation-induced neurobehavioral changes remain elusive. In this study, we aimed to explore the mechanisms underlying UV radiation-induced neurobehavioral changes. In a mouse model, we observed that UV irradiation of the skin induces deficits in hippocampal memory, synaptic plasticity, and adult neurogenesis, as well as increased dopamine levels in the skin, adrenal glands, and brain...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825640/rgs19-activates-the-myh9-%C3%AE-catenin-c-myc-positive-feedback-loop-in-hepatocellular-carcinoma
#33
JOURNAL ARTICLE
Shanjia Ke, Shounan Lu, Yanan Xu, Miaoyu Bai, Hongjun Yu, Bing Yin, Chaoqun Wang, Zhigang Feng, Zihao Li, Jingjing Huang, Xinglong Li, Baolin Qian, Yongliang Hua, Yao Fu, Bei Sun, Yaohua Wu, Yong Ma
Hepatocellular carcinoma (HCC) is one of the most common fatal cancers worldwide, and the identification of novel treatment targets and prognostic biomarkers is urgently needed because of its unsatisfactory prognosis. Regulator of G-protein signaling 19 (RGS19) is a multifunctional protein that regulates the progression of various cancers. However, the specific function of RGS19 in HCC remains unclear. The expression of RGS19 was determined in clinical HCC samples. Functional and molecular biology experiments involving RGS19 were performed to explore the potential mechanisms of RGS19 in HCC...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825639/catching-up-but-still-miles-behind-a-patient-registry-for-otoferlin
#34
LETTER
Barbara Vona, Bernd Wollnik, Nicola Strenzke, Tobias Moser
No abstract text is available yet for this article.
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825638/mettl3-mediated-pre-mir-665-dlx3-m-6-a-methylation-facilitates-the-committed-differentiation-of-stem-cells-from-apical-papilla
#35
JOURNAL ARTICLE
Tingjie Gu, Rong Guo, Yuxin Fang, Ya Xiao, Luyao Chen, Na Li, Xingyun Kelesy Ge, Yijia Shi, Jintao Wu, Ming Yan, Jinhua Yu, Zehan Li
Methyltransferase-like 3 (METTL3) is a crucial element of N6-methyladenosine (m6 A) modifications and has been extensively studied for its involvement in diverse biological and pathological processes. In this study, we explored how METTL3 affects the differentiation of stem cells from the apical papilla (SCAPs) into odonto/osteoblastic lineages through gain- and loss-of-function experiments. The m6 A modification levels were assessed using m6 A dot blot and activity quantification experiments. In addition, we employed Me-RIP microarray experiments to identify specific targets modified by METTL3...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825637/locus-of-il-9-control-il9-epigenetic-regulation-in-cellular-function-and-human-disease
#36
REVIEW
Aran Son, Ishita Baral, Guido H Falduto, Daniella M Schwartz
Interleukin-9 (IL-9) is a multifunctional cytokine with roles in a broad cross-section of human diseases. Like many cytokines, IL-9 is transcriptionally regulated by a group of noncoding regulatory elements (REs) surrounding the IL9 gene. These REs modulate IL-9 transcription by forming 3D loops that recruit transcriptional machinery. IL-9-promoting transcription factors (TFs) can bind REs to increase locus accessibility and permit chromatin looping, or they can be recruited to already accessible chromatin to promote transcription...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38825636/regulation-of-metaplasia-and-dysplasia-in-the-stomach-by-the-stromal-microenvironment
#37
REVIEW
Jared D Rhodes, James R Goldenring, Su-Hyung Lee
Research on the microenvironment associated with gastric carcinogenesis has focused on cancers of the stomach and often underestimates premalignant stages such as metaplasia and dysplasia. Since epithelial interactions with T cells, macrophages, and type 2 innate lymphoid cells (ILC2s) are indispensable for the formation of precancerous lesions in the stomach, understanding the cellular interactions that promote gastric precancer warrants further investigation. Although various types of immune cells have been shown to play important roles in gastric carcinogenesis, it remains unclear how stromal cells such as fibroblasts influence epithelial transformation in the stomach, especially during precancerous stages...
June 3, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38816566/generation-of-a-lethal-mouse-model-expressing-human-ace2-and-tmprss2-for-sars-cov-2-infection-and-pathogenesis
#38
JOURNAL ARTICLE
Gi Uk Jeong, Insu Hwang, Wooseong Lee, Ji Hyun Choi, Gun Young Yoon, Hae Soo Kim, Jeong-Sun Yang, Kyung-Chang Kim, Joo-Yeon Lee, Seong-Jun Kim, Young-Chan Kwon, Kyun-Do Kim
Mouse models expressing human ACE2 for coronavirus disease 2019 have been frequently used to understand its pathogenesis and develop therapeutic strategies against SARS-CoV-2. Given that human TMPRSS2 supports viral entry, replication, and pathogenesis, we established a double-transgenic mouse model expressing both human ACE2 and TMPRSS2 for SARS-CoV-2 infection. Co-overexpression of both genes increased viral infectivity in vitro and in vivo. Double-transgenic mice showed significant body weight loss, clinical disease symptoms, acute lung injury, lung inflammation, and lethality in response to viral infection, indicating that they were highly susceptible to SARS-CoV-2...
May 31, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38806689/lactate-exacerbates-lung-damage-induced-by-nano-microplastics-through-the-gut-microbiota-hif1a-ptbp1-pathway
#39
LETTER
Xin Chen
No abstract text is available yet for this article.
May 29, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38760513/exploiting-sweet-relief-for-preeclampsia-by-targeting-autophagy-lysosomal-machinery-and-proteinopathy
#40
JOURNAL ARTICLE
Zheping Huang, Shibin Cheng, Sukanta Jash, Jamie Fierce, Anthony Agudelo, Takanobu Higashiyama, Nazeeh Hanna, Akitoshi Nakashima, Shigeru Saito, James Padbury, Jessica Schuster, Surendra Sharma
The etiology of preeclampsia (PE), a severe complication of pregnancy with several clinical manifestations and a high incidence of maternal and fetal morbidity and mortality, remains unclear. This issue is a major hurdle for effective treatment strategies. We recently demonstrated that PE exhibits an Alzheimer-like etiology of impaired autophagy and proteinopathy in the placenta. Targeting of these pathological pathways may be a novel therapeutic strategy for PE. Stimulation of autophagy with the natural disaccharide trehalose and its lacto analog lactotrehalose in hypoxia-exposed primary human trophoblasts restored autophagy, inhibited the accumulation of toxic protein aggregates, and restored the ultrastructural features of autophagosomes and autolysosomes...
May 17, 2024: Experimental & Molecular Medicine
journal
journal
32545
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.