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Experimental Animals

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https://read.qxmd.com/read/30760660/a-rat-model-of-serous-borderline-ovarian-tumors-induced-by-7-12-dimethylbenz-a-anthracene
#1
Song-Qi Cai, Ying Li, Yong-Ai Li, Li Wang, Jian Zhu, Shu-Hui Zhao, Xin Li, Jin-Wei Qiang
Serous borderline ovarian tumors (SBOTs) behave between benign cystadenomas and carcinomas, and the effective detection and clinical management of SBOTs remain clinical challenges. Because it is difficult to isolate and enrich borderline tumor cells, a borderline animal model is in need. 7,12- dimethylbenz[a]anthracene (DMBA) is capable of inducing the initiation, promotion, and progression of serous ovarian tumors. This study aims to investigate the proper dosage and induction time of DMBA for rat models of SBOTs, and explore their morphological features demonstrated by magnetic resonance (MR) imaging and molecular genetic characteristics...
February 14, 2019: Experimental Animals
https://read.qxmd.com/read/30760650/continuous-measurement-of-locomotor-activity-during-convalescence-and-acclimation-in-group-housed-rats
#2
Takahiro Yoshizawa, Shin Shimada, Yoshito Takizawa, Tsuyoshi Makino, Yasuhide Kanada, Yoshiharu Ito, Toshiaki Ochiai, Kiyoshi Matsumoto
Locomotor activity is affected by a range of factors in addition to experimental treatment, including the breeding environment. Appropriate convalescence and acclimation are important for animal experiments, because environmental changes and physical burden can result from surgery, transportation, and cage exchange. However, the duration that locomotor activity is affected by these factors is currently unclear, because it has traditionally been difficult to measure locomotor activity in multiple group-housed animals in any location other than the analysis room...
February 13, 2019: Experimental Animals
https://read.qxmd.com/read/30745527/species-differences-in-lipoprotein-lipase-and-hepatic-lipase-activities-comparative-studies-of-animal-models-of-lifestyle-related-diseases
#3
Norie Kimura, Akio Kikumori, Daisuke Kawase, Mitsumasa Okano, Kumiko Fukamachi, Tatsuro Ishida, Katsuyuki Nakajima, Masashi Shiomi
Lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) have an important role in lifestyle-related diseases. To evaluate species differences, we compared LPL and HTGL activities in different animal models of lifestyle-related diseases using the same assay kit. Normal animals (JW rabbits, ICR mice, and SD rats), a hypercholesterolemic animal model (WHHLMI rabbits), and obese animal models (KK-Ay mice and Zucker fatty rats) fed standard chow were used in this study. Plasma was prepared before and after an intravenous injection of heparin sodium under fasting and feeding...
February 11, 2019: Experimental Animals
https://read.qxmd.com/read/30643078/fetal-and-early-postnatal-development-of-the-porcine-tonsils-of-the-soft-palate
#4
Shunichi Suzuki, Daiichiro Fuchimoto
Tonsils are mucosa-associated lymphoid tissues located at the openings of the gastrointestinal and respiratory tracts, which play a key role in the surveillance of inhaled or ingested pathogens and can concurrently be reservoirs of infectious agents. Therefore, tonsils are important for the immunology and hygiene management of domestic animals, including pigs. However, the process of their fetal developmental has been poorly described, at least in part, because rodents lack tonsils. Therefore, we performed a histological analysis of porcine tonsils of the soft palate from 60 to 100 days of gestation (DG) and from 2 to 14 days post partum (DP)...
January 14, 2019: Experimental Animals
https://read.qxmd.com/read/30606939/protective-effects-of-sulforaphane-on-diabetic-retinopathy-activation-of-the-nrf2-pathway-and-inhibition-of-nlrp3-inflammasome-formation
#5
Sheng Li, Hongwei Yang, Xiaolong Chen
Sulforaphane (SFN) is abundant in cruciferous plants, providing significant protection against many chronic diseases. With the aim of clarifying the efficacy of sulforaphane in diabetic retinopathy (DR), a series of systematic studies were carried out in the present study. Male Sprague Dawley rats were intraperitoneally injected with streptozotocin (STZ, 65 mg/kg), and those with confirmed diabetes mellitus were given different doses of SFN (0.5 and 1 mg/kg/d) for 12 weeks. In vitro, Müller cells exposed to 25 mM glucose were treated with 2...
January 1, 2019: Experimental Animals
https://read.qxmd.com/read/30587671/mir-206-3p-alleviates-chronic-constriction-injury-induced-neuropathic-pain-through-targeting-hdac4
#6
Jing Wen, Tao He, Fangfang Qi, Hongping Chen
It was identified that microRNAs were involved in the regulation of chronic neuropathic pain. However, the role of miR-206-3p in neuropathic pain was still unclear. In the current study, the role of miR-206-3p, a type of mature miR-206, in neuropathic pain was investigated. The potential mechanisms were also explored. We found that the expression of miR-206-3p decreased in the dorsal root ganglion (DRG) of chronic constriction sciatic nerve injury (CCI) rats, whereas the While histone deacetylase 4 (HDAC4) level increased...
December 26, 2018: Experimental Animals
https://read.qxmd.com/read/30584201/effects-of-housing-conditions-on-behaviors-and-biochemical-parameters-in-juvenile-cynomolgus-monkeys-macaca-fascicularis
#7
Hironari Koyama, Yuki Tachibana, Kaoru Takaura, Shigetoshi Takemoto, Kiyoshi Morii, Sou Wada, Hayato Kaneko, Mayuko Kimura, Atsushi Toyoda
To investigate the effects of environmental enrichment on laboratory monkeys, we studied behavioral and physiological differences following changes in housing conditions. Ten male and female juvenile cynomolgus monkeys were first housed in pairs for 8 weeks after quarantine/acclimatization (singly housed) and subsequently housed alone for the next 8 weeks. Monkeys were subjected to evaluations of body weight gain, stereotypic or affiliative behaviors, cortisol, 4-ethylphenyl sulfate (4EPS) and catecholamine concentrations in biological samples, and blood chemistry tests under both housing conditions...
December 21, 2018: Experimental Animals
https://read.qxmd.com/read/30542001/genetic-diversity-of-21-experimental-chicken-lines-with-diverse-origins-and-genetic-backgrounds
#8
Mitsuo Nunome, Keiji Kinoshita, Satoshi Ishishita, Yasushige Ohmori, Atsushi Murai, Yoichi Matsuda
The genetic characteristics and diversity of 21 experimental chicken lines registered with the National BioResource Project of Japan were examined using mitochondrial D-loop sequences and 54 microsatellite DNA markers. A total of 12 haplotypes were detected in the 500-bp mitochondrial DNA sequences of the hypervariable segment I for 349 individuals of 21 lines. The 12 haplotypes belonged to three (A, D, and E) haplogroups, out of the seven (A-H) common haplogroups in domestic chickens and red junglefowls. The haplogroups A and D were widely represented in indigenous chickens in the Asian and Pacific regions, and the haplogroup E was the most prevalent in domestic chickens...
December 13, 2018: Experimental Animals
https://read.qxmd.com/read/30531118/hatano-rats-selectively-bred-for-high-and-low-avoidance-learning-an-overview
#9
Ryo Ohta, Kohich Kojima
This review compiles the results of a series of studies on Hatano high- and low-avoidance animals (HAA and LAA, respectively) established at the Hatano Research Institute, Food and Drug Safety Center, Japan. The HAA and LAA lines were selected and bred from Sprague Dawley rats for high and low avoidance learning, respectively, in a shuttlebox task since 1985. Although Hatano rats were selected only based on their behavioral traits in the active avoidance task, strain differences between the HAA and LAA lines were also observed in their stress responses and reproductive functions...
December 10, 2018: Experimental Animals
https://read.qxmd.com/read/30531117/the-acute-pulmonary-toxicity-in-mice-induced-by-staphylococcus-aureus-particulate-matter-and-their-combination
#10
Fan Wang, Ruiling Wang, Haifang Liu
Inhalation of pathogenic bacteria transported by particulate matter (PM) presents an important potential threat to human health. Therefore, the pulmonary toxicity in mice caused by Staphylococcus aureus (S. aureus) and PM as individual matter and mixtures was studied. PM and S. aureus were instilled intratracheally into Kunming mice at doses of 0.2 mg/mouse and 5.08 × 106 CFU /mouse, respectively, as individual matter and in combination two times at 5-day intervals. After the exposure period, oxidative stress markers and nitric oxide (NO) in the lung, cellular infiltration, neurotrophins, chemokines, andcytokines in bronchoalveolar lavage fluid (BALF), and immunoglobulin (Ig) in sera were examined...
December 10, 2018: Experimental Animals
https://read.qxmd.com/read/30531093/a-semi-nested-rt-pcr-assay-for-detection-of-norovirus-in-rat-fecal-samples
#11
Josélia Cristina de Oliveira Moreira, Daniele Masselli Rodrigues, Rovilson Gilioli, Fernando Ferreira Costa, Dulcinéia Martins Albuquerque
Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infection in laboratory rats has not yet been reported. In this study, we describe the development of a sensitive, semi-nested RT-PCR assay for detection of norovirus in fecal samples from Rattus norvegicus, reared in animal facilities under different sanitary barrier conditions...
December 7, 2018: Experimental Animals
https://read.qxmd.com/read/30487357/aim-deficient-mouse-fed-a-high-trans-fat-high-cholesterol-diet-a-new-animal-model-for-nonalcoholic-fatty-liver-disease
#12
Ginga Komatsu, Toru Nonomura, Mai Sasaki, Yuki Ishida, Satoko Arai, Toru Miyazaki
Owing to changes in lifestyle, nonalcoholic fatty liver disease (NAFLD) is becoming a common form of chronic liver injury. NAFLD comprises a wide variety of disease stages, from simple steatosis to nonalcoholic steatohepatitis, which is a risk factor for the development of hepatocellular carcinoma (HCC). Because animal models for NAFLD are needed to investigate the precise pathogenesis, we aimed to establish a new mouse model employing mice deficient for apoptosis inhibitor of macrophage (AIM-/- ), which exhibit accelerated lipid storage in the liver and high susceptibility to developing HCC in response to a high-fat diet (HFD)...
November 28, 2018: Experimental Animals
https://read.qxmd.com/read/30393276/chronic-restraint-stress-impairs-sociability-but-not-social-recognition-and-spatial-memory-in-c57bl-6j-mice
#13
Mohd Aizat Zain, Vijayapandi Pandy, Abu Bakar Abdul Majeed, Won Fen Wong, Zahurin Mohamed
Chronic stress has been associated with impairment of memory, learning, and social cognition. In animal studies, chronic stress has been shown to impair rodent sociability behaviour which mimics social withdrawal as observed in depression patients. The effect of chronic stress on social recognition, however, is uncertain. Moreover, with reference to spatial learning and memory, the effect of chronic stress is dependent on the type of behavioural task: an appetitively or aversively motivated tasks. The effect of chronic stress was consistent in impairing spatial learning and memory in the appetitive task; however, the effect was inconsistent in an aversive task like the Morris water maze...
November 5, 2018: Experimental Animals
https://read.qxmd.com/read/30068825/carnosol-protects-against-renal-ischemia-reperfusion-injury-in-rats
#14
Yi Zheng, Yong Zhang, Yichun Zheng, Nan Zhang
Acute kidney injury, which is caused by renal ischemia-reperfusion injury (IRI), occurs in several clinical situations and causes severe renal damage. There is no effective therapeutic agent available for renal IRI at present. In this study, we performed an experiment based on an in vivo murine model of renal IRI to examine the effect of carnosol. Thirty Sprague-Dawley rats were randomized into three groups (10 rats in each group): the sham, IRI, and carnosol groups. Rats in the carnosol group were injected intravenously with 3 mg/kg of carnosol, and those in the sham and IRI groups were injected intravenously with 10% dimethyl sulfoxide 1 h before ischemia...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/30068792/swine-model-of-in-stent-stenosis-in-the-iliac-artery-evaluating-the-serial-time-course
#15
Osamu Ishikawa, Minoru Tanaka, Kenjiro Konno, Terumitsu Hasebe, Ayumi Horikawa, Akira Iijima, Nobuhito Saito, Koki Takahashi
The aim of this study was to propose a new animal model evaluating the serial time course of in-stent stenosis by repeated carotid artery catheterization in the same animal. 16 bare-metal stents were implanted in the normal external and internal iliac artery of 8 miniature pigs. Repeated measurements were performed in the same animal every 2 weeks for 12 weeks through carotid artery catheterization. The time course and peak neointimal proliferation were evaluated by intravascular ultrasound. Health of all animals was assessed by clinical and hematological examinations...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/30012936/electroporation-mediated-genome-editing-in-vitrified-warmed-mouse-zygotes-created-by-ivf-via-ultra-superovulation
#16
Yoshiko Nakagawa, Tetsushi Sakuma, Toru Takeo, Naomi Nakagata, Takashi Yamamoto
Recently, genome editing in mouse zygotes has become convenient and scalable, in association with various technological developments and improvements such as novel nuclease tools, alternative delivery methods, and contemporary reproductive engineering techniques. We have so far demonstrated the applicability of ultra-superovulation, in vitro fertilization (IVF), and vitrification/warming of zygotes in microinjection-mediated mouse genome editing. Moreover, an electroporation-mediated method has rapidly become established for simple gene knockout and small precise modifications including single amino acid substitutions...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/29998917/measurements-of-body-surface-area-and-volume-in-laboratory-rabbits-new-zealand-white-rabbits-using-a-computed-tomography-scanner
#17
Tadashi Itoh, Mifumi Kawabe, Takahiko Nagase, Tsuneo Koike, Masafumi Miyoshi, Kazuro Miyahara
The body surface area (BSA) of an organism is one of the important parameters for evaluating physiological functions. In drug development, normalization by BSA is an appropriate method for extrapolating doses between species. The BSA of animals has generally been estimated by multiplying the k value by 2/3 of the power of the body weight (BW) (Meeh's formula). In mathematics, if it is assumed that the density and body shape of the animals are essentially constant, the BSA is proportional to BW2/3 . In this study, we measured the BSA and volume (V) of 72 laboratory rabbits (48 males and 24 females of New Zealand White rabbits [NZW]), using a computed tomography scanner...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/29973470/effect-of-catha-edulis-khat-on-pancreatic-functions-in-streptozotocin-induced-diabetes-in-male-sprague-dawley-rats
#18
Abdulsamad Alsalahi, Mohammed A Alshawsh, Zamri Chik, Zahurin Mohamed
People consume Catha edulis (khat) for its euphoric effect, and type 1 diabetics have claimed that khat could reduce elevated levels of blood sugar. However, khat has been suggested to provoke diabetes mellitus through destruction of pancreatic β-cells. This study investigated the effect of an ethanolic khat extract on pancreatic functions in type 1 diabetes (T1DM)-induced male Sprague-Dawley rats and to assess its in vitro cytotoxicity in rat pancreatic β-cells (RIN-14B). T1DM was induced in a total of 20 rats with a single intraperitoneal injection of 75 mg/kg of streptozotocin...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/29973435/mito-mice%C3%A2-and-mitochondrial-dna-mutator-mice-as-models-of-human-osteoporosis-caused-not-by-aging-but-by-hyperparathyroidism
#19
Takayuki Mito, Haruna Tani, Michiko Suzuki, Kaori Ishikawa, Kazuto Nakada, Jun-Ichi Hayashi
Mitochondrial DNA (mtDNA) mutator mice showing accelerated accumulation of mtDNA with somatic mutations are potentially useful models of human aging, whereas mito-miceΔ showing accelerated accumulation of mtDNA with a deletion mutation (ΔmtDNA) are potentially useful models of mitochondrial diseases but not human aging, even though both models express an age-associated decrease in mitochondrial respiration. Because osteoporosis is the only premature aging phenotype observed in mtDNA mutator mice with the C57BL/6J nuclear genetic background, our previous study precisely examined its expression spectra and reported that both mtDNA mutator mice and mito-miceΔ, but not aged mice, developed decreased cortical bone thickness...
November 1, 2018: Experimental Animals
https://read.qxmd.com/read/29937473/protective-effect-of-a-mechanistic-target-of-rapamycin-inhibitor-on-an-in-vivo-model-ofcisplatin-induced-ovarian-gonadotoxicity
#20
Yuji Tanaka, Fuminori Kimura, Luyi Zheng, Shoji Kaku, Akie Takebayashi, Kyoko Kasahara, Shunichiro Tsuji, Takashi Murakami
This study aimed to evaluate the protective effect of everolimus, a mechanistic target of rapamycin (mTOR) inhibitor, on cisplatin chemotherapy-induced ovarian toxicity. Eighty sexually mature, virgin, female, 7-week-old C57BL/6J mice were divided into four groups: control, cisplatin (Cis), everolimus (mTORi), and everolimus plus cisplatin (mTORi+Cis). Mice in the Cis and mTORi+Cis groups were intraperitoneally injected with 2 mg/kg of cisplatin for 15 d. Mice in the mTORi and mTORi+Cis groups were orally administered 2...
November 1, 2018: Experimental Animals
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