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Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology

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https://read.qxmd.com/read/30735775/tyrosine-hydroxylase-is-crucial-for-pupal-pigmentation-in-zeugodacus-tau-walker-diptera-tephritidae
#1
He-He Zhang, Qi-Wen Zhang, Atif Idrees, Jia Lin, Xue-Sen Song, Qing-E Ji, Ying-Gang Du, Min-Lin Zheng, Jia-Hua Chen
Tyrosine hydroxylase (TH) is the initial enzyme responsible for cuticle sclerotization and pigmentation in many insect species, but to date, no direct functional studies have focused on TH in Zeugodacus tau. Here, the 3336-bp full-length cDNA of TH was isolated from Z. tau, a notorious horticultural pest infesting fruits and vegetables. qRT-polymerase chain reaction revealed that ZtTH transcripts were highly abundant at the time of pupal tanning and during adult emergence and were expressed in the midgut, integument and head of molting larvae...
February 5, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30735774/the-expression-pattern-of-heat-shock-protein-90ab-hsp90ab-and-stress-inducible-protein-1-stip1-during-spermatogenesis-of-mudskipper-boleophthalmus-pectinirostris-with-discussion-of-the-potential-environmental-biomarker
#2
Chen Du, Ying-Li Han, Cong-Cong Hou, Jun-Quan Zhu
Heat shock protein 90 (HSP90), which functions as a molecular chaperone, plays an important role in reproduction and cellular defense. Among the HSP90 family, HSP90AB is an important isoform. Stress-inducible protein 1 (Stip1) acts as a co-chaperone that mediates interactions with HSP90 and appears to be a player in spermatogenesis and stress response. However, the functions of both HSP90 and Stip1 during spermatogenesis and heat stress response in Boleophthalmus pectinirostris remain unknown. We investigated mRNA expression patterns of HSP90AB and Stip1 under heat stress conditions...
February 5, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30735773/a-functional-gene-encoding-carnitine-palmitoyltransferase-1-and-its-transcriptional-and-kinetic-regulation-during-fasting-in-large-yellow-croaker
#3
Cheng-Cheng Wang, Lan-Fang Si, Wei-Ye Li, Jia-Lang Zheng
Carnitine palmitoyltransferase 1 (CPT1) plays an essential role in maintaining energy supply via fatty acid oxidation, especially under fasting. In this study, the complete cDNA sequence of cpt1a was cloned from liver of large yellow croaker (Larimichthys crocea), with an open reading frame of 2319 bp encoding a protein of 772 amino acids. Bioinformatics analysis predicted the presence of conserved functional motifs and amino acid residues. The highest mRNA expression of cpt1a was observed in the liver. Phylogenetic tree clearly shows that CPT1A protein is a homologue of mammalian CPT1A...
February 5, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30710691/the-effects-of-intraperitoneal-administration-of-francisella-noatunensis-subsp-noatunensis-on-hepatic-intermediary-metabolism-and-indicators-of-stress-in-patagonian-blennie-eleginops-maclovinus
#4
R Oyarzún, C Vargas-Lagos, D Martínez, J L P Muñoz, L P Dantagnan, L Vargas-Chacoff
Francisellosis is a disease produced by Francisella spp. which affects farmed fish. Eleginops maclovinus specimens can be caught close to salmon farming centers, feeding on un-consumed pellet, making the transmission of pathogens such as Francisella noatunensis possible. The aim of this study was to evaluate the effect of F. noatunensis on liver intermediary metabolism in E. maclovinus. 144 fish were injected intraperitoneally with F. noatunensis at a low dose LD (1 × 101 cells/μL), medium dose MD (1 × 105 cells/μL), high dose HD (1 × 1010 cells/μL), or with culture medium C (control), and sampled at 1, 3, 7, 14, 21 and 28 days post injection (dpi)...
January 30, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30703525/a-molecular-survey-of-programmed-cell-death-in-rainbow-trout-structural-and-functional-specifications-of-apoptotic-key-molecules
#5
Marieke Verleih, Andreas Borchel, Alexander Rebl, Julia Brenmoehl, Carsten Kühn, Tom Goldammer
Apoptosis is an integral part of homeostasis and supports multiple physiological processes such as development and immune defense, thereby directly targeting damaged or unwanted cells without affecting neighbor cells. In the present study, we characterized the apoptotic key factors caspase-3, -7, and - 8 as well as regulator protein TPT1 (translationally-controlled tumor protein 1) from rainbow trout (Oncorhynchus mykiss). We identified multiple single-nucleotide changes in their coding sequences and showed that the CASP3 gene is present in at least three variants...
January 28, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30695731/exposure-to-aeromonas-hydrophila-induces-inflammation-and-increases-expression-of-the-gene-encoding-for-a-putative-dual-ctld-containing-lectin-in-milkfish-liver
#6
Lorenz Rhuel P Ragasa, Jaime Lorenzo N Dinglasan, Imee Rose E Felipe, Zubaida U Basiao, Michael C Velarde
Milkfish (Chanos chanos Forsskal) is an important aquaculture product and is the sole extant species of the family Chanidae (order Gonorynchiformes). While there are already several reports regarding milkfish aquaculture, studies on milkfish immunity and gene expression are very limited. In this study, we showed that Aeromonas hydrophila induces inflammation in milkfish liver. We identified a milkfish C-type lectin-like domain containing proteins (CTLDcps) gene, designated as CcClec, which was upregulated in respond to A...
January 26, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30690199/the-response-of-nitric-oxide-system-to-high-altitude-in-phrynocephalus-erythrurus-on-qinghai-tibetan-plateau
#7
Ying Xin, Yan Wang, Huihui Wang, Xiaolong Tang, Yucheng Bai, Songsong Lu, Ying Xin
Nitric oxide (NO), produced by nitric oxide synthases (NOS) from L-arginine, plays important roles in a wide range of physiological processes. However, little is known about ectothermic species. To investigate the response of NO/NOS system in adaptation to different altitudes in Phrynocephalus genus, the red tail toad-headed lizard Phrynocephalus erythrurus that live at 4500-5300 m on the Qinghai-Tibet Plateau and another low altitude living lizard Phrynocephalus przewalskii were selected in the present study...
January 25, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30677513/dietary-methionine-increased-the-lipid-accumulation-in-juvenile-tiger-puffer-takifugu-rubripes
#8
Houguo Xu, Qinggong Zhang, Yuliang Wei, Zhangbin Liao, Mengqing Liang
Methionine (Met) is one of the most important amino acids in fish feed. The effects of dietary Met on lipid deposition in fish varied a lot among different studies. The present study was aimed at investigating the effects of dietary Met supplementation on the lipid accumulation in tiger puffer, which have a unique lipid storage pattern. Crystalline L-Met was supplemented to a low-fishmeal control diet to obtain two experimental diets with a low (1.1% of dry weight, L-MET) or high Met level (1.6% of dry weight, H-MET)...
January 21, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30665028/molecular-cloning-of-il-6-il-10-il-11-ifn-%C3%A9-and-modulation-of-pro-and-anti-inflammatory-cytokines-in-cobia-rachycentron-canadum-after-photobacterium-damselae-subsp-piscicida-infection
#9
Hung B Tran, Shih-Chu Chen, Hso-Chi Chaung, Ta-Chih Cheng
Photobacterium damselae subsp. piscicida (P. damselae subsp. piscicida) is the agent of Photobacteriosis, a serious fish disease that produces an acute infection and high mortality in farmed cobia. It has been proved that regulation of pro- and anti-inflammatory cytokines play a central role in initiation of proper inflammatory responses against bacterial infection. Here we have analyzed the expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-8 and IFN-ɤ) and anti-inflammatory cytokines (IL-10 and IL-11) in spleen and head kidney during acute P...
January 18, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30665027/cloning-and-expression-of-the-lifespan-associated-protein-sir2-from-daphnia-pulex
#10
Chongyuan Lin, Mengdi Liu, Xiaojing Zhu, Mengmeng Zhang, Shanliang Xu, Danli Wang, Yunlong Zhao
The activity of NAD+ -dependent deacetylase Sir2, originally discovered in yeast, is known to be essential for effective longevity. The relationship between Sir2 and lifespan in Daphnia pulex was investigated by cloning and analysis the full-length 1901 bp cDNA. The Sir2 protein includes several zinc finger active sites and two readily hydrolysable low-complexity SD-rich regions. The three-domain structure of Sir2 has a distinct crevice that plays an important regulatory role in the binding of NAD+ . D. pulex Sir2 shares 90% amino acid sequence identity with Sir2 from D...
January 18, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30665026/feedback-regulation-of-17%C3%AE-estradiol-on-two-kisspeptin-genes-in-the-dabry-s-sturgeon-acipenser-dabryanus
#11
Hua-Mei Yue, Huan Ye, Rui Ruan, Hao Du, Chuang-Ju Li, Qiwei Wei
In tetrapods, kisspeptins are a group of peptides that play essential roles in the regulation of the Gonadotropin-releasing hormone secretion, and may participate in the feedback regulation of sex steroids as well. In this study, two kiss paralogs, designated as dskiss1 and dskiss2 were identified in Acipenser dabryanus. The full-length cDNA sequences of dskiss1 and dskiss2 are 1265 and 744 base pairs (bp), encoding 130 and 146 amino acids, respectively. Multiple sequence alignment indicated that both Kiss1 and Kiss2 decapeptides were highly conserved among vertebrates...
January 18, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30641133/characterization-and-in-vitro-expression-of-arginine-kinase-gene-in-the-invasive-western-flower-thrips-frankliniella-occidentalis
#12
Fan Dong, Nan Zhang, Zhijuan Xie, Xiangkun Meng, Kun Qian, Caihong Ji, Mingxing Lu, Yuzhou Du, Jianjun Wang
Arginine kinase (AK) plays a critical role in insect energy metabolism and has been proposed to be a potential insecticide target for commercial exploitation. In this study, the full length cDNA encoding a typical group 1 insect AK (FoAK) was isolated from the western flower thrips (WFT), Frankliniella occidentalis (Pergande). Sequence analysis showed that FoAK contains an open reading frame of 1068 nucleotides, which encods a protein of 355 amino acid residues including the signature sequence pattern of ATP-guanidino kinases...
January 11, 2019: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30594644/cloning-of-fatty-acid-binding-protein-2-fabp2-in-loach-misgurnus-anguillicaudatus-and-its-expression-in-response-to-dietary-oxidized-fish-oil
#13
Yin Zhang, Xiaojuan Cao, Jian Gao
Fatty acid-binding protein 2 (FABP2) is involved in the uptake of dietary fatty acids and intracellular fatty acid transport. In the present study, cDNA of fabp2 in loach (Misgurnus anguillicaudatus) was cloned and its full length was 956 bp, encoding 134 amino acids. Gene expression of fabp2 was investigated in different development stages and different tissues of loach, showing that the expression of fabp2 was recorded at 2 days after hatching (DAH), 10DAH, 20DAH and 35DAH, and higher in loach intestine, muscle and brain, compared with other tissues...
December 27, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30590176/molecular-characterization-and-expression-analysis-of-a-germ-cell-marker-vasa-in-marbled-goby-oxyeleotris-marmorata
#14
Wei Liu, Hong Zhang, Yangxi Xiang, Kuntong Jia, Mingfei Luo, Meisheng Yi
Identification of germ cell markers is important for investigating reproduction biology in fish. Vasa is one of the most studied germ cell marker in mammals and lower vertebrates including fish. Here, we cloned the vasa homologue from the freshwater fish marbled goby (Oxyeleotris marmorata), termed omvasa. The full length cDNA of omvasa was 2344 bp and encoded a deduced protein of 658 amino acids (aa), which shared high identities with vasa homologues from other vertebrates. The deduced omvasa protein contained fifteen RG/RGG repeats at N-terminus, two ATPase motifs, and C-terminal RNA unwinding and RNA binding domains...
December 24, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30590175/transcriptional-activation-of-genes-involved-in-oxidative-stress-in-salmo-salar-challenged-with-piscirickettsia-salmonis
#15
A V F Pedro, D Martinez, J P Pontigo, C Vargas-Lagos, C Hawes, S Wadsworth, F J Morera, L Vargas-Chacoff, A J Yañez
Piscirickettsiosis caused by Piscirickettsia salmonis constitutes one of the main problems in farmed salmonid and marine fishes. The objective of this study was to evaluate the modulation of genes involved in the oxidative stress in the liver and muscle of Salmo salar challenge with low dosage of P. salmonis. The treatment (in duplicate) were as follows: Control injection (culture medium) and P. salmonis injection (1 × 102 PFU/mL) with sampling (liver and muscle) at several time-points during the 42-days experimental period (dpi)...
December 24, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30557612/the-effect-of-dietary-carotenoid-increase-on-painted-turtle-spot-and-stripe-color
#16
John E Steffen, Jessica Hultberg, Stephen Drozda
Determination of how carotenoid-based color patches change with pigmentary access is important to understanding color patch function in animals. We performed a carotenoid addition experiment on male painted turtles (Chrysemys picta) to determine if an increase in dietary carotenoids led to location-specific changes in painted turtle spot and stripe color. Turtles fed large quantities of carotenoids had increased yellow chroma in chin stripes and increased red chroma in the neck and carapace stripes, as well as reduced ultraviolet (UV) chroma in the neck (orange to the human eye) and fore -limb (red to the human eye) stripes...
December 14, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30528668/silencing-of-ppar%C3%AE-bb-mrna-in-brown-trout-primary-hepatocytes-effects-on-molecular-and-morphological-targets-under-the-influence-of-an-estrogen-and-a-ppar%C3%AE-agonist
#17
Tânia Vieira Madureira, Ivone Pinheiro, Fernanda Malhão, L Filipe C Castro, Eduardo Rocha, Ralph Urbatzka
The crosstalk between peroxisome proliferator-activated receptor α (PPARα) and estrogenic pathways are shared from fish to humans. Salmonid fish had an additional genome duplication, and two PPARα isoforms (PPARαBa and PPARαBb) were previously identified. Since a negative regulation between estrogen signaling and PPARα was described, a post-transcriptional gene silencing for PPARαBb was designed in primary brown trout hepatocytes. The aims of the study were to: (i) decipher the effects of PPARαBb knock-down on peroxisome morphology and on mRNA expression of potential target genes, and (ii) to assess the cross-interferences caused by an estrogenic compound (17α-ethinylestradiol - EE2) and a PPARα agonist (Wy-14,643 - Wy) using the established knock-down model...
December 7, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30502388/naa15-knockdown-enhances-c2c12-myoblast-fusion-and-induces-defects-in-zebrafish-myotome-morphogenesis
#18
Olivier Monestier, Aurélie Landemaine, Jérôme Bugeon, Pierre-Yves Rescan, Jean-Charles Gabillard
The understanding of muscle tissue formation and regeneration is essential for the development of therapeutic approaches to treat muscle diseases or loss of muscle mass and strength during ageing or cancer. One of the critical steps in muscle formation is the fusion of muscle cells to form or regenerate muscle fibres. To identify new genes controlling myoblast fusion, we performed a siRNA screen in c2c12 myoblasts. The genes identified during this screen were then studied in vivo by knockdown in zebrafish using morpholino...
November 29, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30502389/molecular-cloning-expression-pattern-of-%C3%AE-carotene-15-15-dioxygenase-gene-and-association-analysis-with-total-carotenoid-content-in-pearl-oyster-pinctada-fucata-martensii
#19
Chao Lei, Junhui Li, Zhe Zheng, Xiaodong Du, Yuewen Deng
β-carotene-15,15-dioxygenase is an enzyme involved in carotenoid metabolism to catalyze oxidative cleavage of β-carotene at its central double bond to two molecules of retinal in intestinal cells of vertebrate. In this study, we cloned and characterized β-carotene-15,15-dioxygenase in pearl oyster Pinctada fucata martensii (PmβCDOX). The full length of PmβCDOX gene was 1802 bp, including 1554 bp of the open reading frame (ORF) that encoded 517 amino acids, a 5'UTR of 134 bp and a 3' UTR of 114 bp...
November 28, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://read.qxmd.com/read/30496816/developmental-dynamics-of-myogenesis-in-yesso-scallop-patinopecten-yessoensis
#20
Xiujun Sun, Yanxin Zheng, Tao Yu, Biao Wu, Zhihong Liu, Liqing Zhou, Jiteng Tian, Aiguo Yang
The development of bivalves has been extensively studied over the last 150 years. Despite this, the developmental dynamics of myogenesis in bivalves remains largely unknown, particularly at the early developmental stages. In the present study, we investigate the characteristics of muscle development of Yesso scallop Patinopecten yessoensis by phalloidin staining, light, electron and confocal microscopy. Myoblasts containing chaotic myofilaments are initially found in the blastocoel of trochophore, and become more organized during the transformation from trochophore into veliger...
November 26, 2018: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
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