Rebecca J Haugen, Catherine Barnier, Nathan D Elrod, Hua Luo, Madeline K Jensen, Ping Ji, Craig A Smibert, Howard D Lipshitz, Eric J Wagner, P Lydia Freddolino, Aaron C Goldstrohm
The sequence-specific RNA-binding protein Pumilio controls Drosophila development; however, the network of mRNAs that it regulates remains incompletely characterized. In this study, we utilize knockdown and knockout approaches coupled with RNA-Seq to measure the impact of Pumilio on the transcriptome of Drosophila cells in culture. We also use an improved RNA co-immunoprecipitation method to identify Pumilio-bound mRNAs in Drosophila embryos. Integration of these datasets with the locations of Pumilio binding motifs across the transcriptome reveal novel direct Pumilio target genes involved in neural, muscle, wing, and germ cell development, and cellular proliferation...
April 16, 2024: RNA