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International Journal of Biochemistry & Cell Biology

Yazmin Brown, Susan Hua, Pradeep S Tanwar
The identity of cancer stem cells (CSCs) remains an enigma, with the question outstanding of whether CSCs are fixed entities or plastic cell states in response to microenvironmental cues. Recent evidence highlights the power of the tumor microenvironment to dictate CSC functionality and spatiotemporal regulation that gives rise to tumor heterogeneity. This microenvironmental regulation of CSCs parallels that of normal tissues, whereby resident stem cells reside within specialized microenvironments or 'niches', which provide the cellular and molecular signals that wire every aspect of stem cell behavior and fate...
February 8, 2019: International Journal of Biochemistry & Cell Biology
Saurabh Mandal, Frank Arfuso, Gautam Sethi, Arun Dharmarajan, Sudha Warrier
Breast cancer is a leading cause of mortality in women across the globe. The major reason for its recurrence and high mortality is due to the presence of a drug refractory and self-renewing population of cells, the cancer stem cells (CSCs). Mesenchymal stem cells (MSCs) have recently emerged as a promising cell-based therapeutic agent for the treatment of different cancer types. However, the anti-tumor effect of MSCs has been indicated to be substantially reduced by their in vivo tumor-trophic migration property and direct cell-to-cell integration with tumor stromal elements...
February 5, 2019: International Journal of Biochemistry & Cell Biology
S Rajashree Nandagopalan, Subramaniam Agatheeswaran, Yellamandayya Vadlamudi, Sutapa Biswas, Ghanshyam Biswas, Naresh Chandra Pattnayak, Soumen Chakraborty
Altered or aberrant expression of several splicing factors leads to the progression of different cancers. Though there are several ongoing studies underscoring the role of the splicing regulator polypyrimidine tract binding protein 2 (PTBP2) in neuronal cells, we unveil the role of PTBP2 in chronic myeloid leukemia (CML). Different RNA binding proteins (RBP's) earlier reported in chronic myeloid leukemia blast crisis (CML-BC) cases (n=28) from Radich oncomine leukemia dataset, were compared. We observed increased expression of MSI2 followed by PTBP2 in BC cases and increased PTBP2 expression in relapsed cases (n=10) from the same dataset compared to other RBP's...
February 3, 2019: International Journal of Biochemistry & Cell Biology
Zhantao Tian, Lv Yao, Yongqing Shen, Xiaoqiang Guo, Xianglin Duan
Erythropoietin (EPO) is a secreted hormone that stimulates the production of red blood cells, and the level of EPO is increased under hypoxia. The expression of EPO is regulated not only by the hypoxia-inducible factor (HIF) but also partly through epigenetic modifications, including histone acetylation and methylation. In this study, we report that histone H3K9 demethylase JMJD1 A is regulated by HIF-2α in HepG2 cells under hypoxia. Knockdown or over-expression of JMJD1 A can decrease or increase EPO expression, respectively...
February 1, 2019: International Journal of Biochemistry & Cell Biology
Kai Yan, Yong Fu, Nan Zhu, Zhuo Wang, Jin-Ling Hong, Yao Li, Wei-Jing Li, Hai-Bin Zhang, Jing-Hai Song
BACKGROUND: Immunotherapy is a promising method for the treatment of hepatocellular carcinoma (HCC), in which CD8+ T cells play a key role. The influence of long noncoding RNA (lncRNA) nuclear-enriched autosomal transcript 1(NEAT1) on the antitumor activity of CD8+ T cells was clarified in this study. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from HCC patients, and the expressions of NEAT1 and Tim-3 were determined by qRT-PCR and western blot, respectively...
January 30, 2019: International Journal of Biochemistry & Cell Biology
Bo Liu, Lin Lin, Saima Riazuddin, Ahmed Zubair, Li Wang, Li-Jun Di, Rui Li, Ting-Ting Dong, Chu-Xia Deng, Wei-Min Tong
The role of protein phosphatase 2ACα (PP2ACα) in brain development is poorly understood. To understand the function of PP2ACα in neurogenesis, we inactivated Pp2acα gene in the central nervous system (CNS) of mice by Cre/LoxP system and generated the PP2ACα deficient mice (designated as the Pp2acα-/- mice). PP2ACα deletion results in DNA damage in neuroprogenitor cells (NPCs), which impairs memory formation and cortical neurogenesis. We first identify that PP2ACα can directly associate with Ataxia telangiectasia mutant kinase (ATM) and Ataxia telangiectasia/Rad3-related kinase (ATR) in neocortex and NPCs...
January 30, 2019: International Journal of Biochemistry & Cell Biology
Abhijeet Deshmukh, Frank Arfuso, Philip Newsholme, Arun Dharmarajan
The expression and levels of secreted frizzled-related proteins (sFRPs), important Wnt signalling antagonists, have been reported to be reduced in various cancers, and are associated with disease progression and poor prognosis. During tumour development, all sFRP (1, 2, 3, 4, and 5) genes are hypermethylated, causing transcriptional silencing. sFRPs have an ability to sensitize tumour cells to chemotherapeutic drugs, enhancing cell death. Reduced Wnt signalling is associated with loss of cancer stem cell (CSC) viability...
January 30, 2019: International Journal of Biochemistry & Cell Biology
Yudi Han, Jing Ren, Yun Bai, Xuetao Pei, Yan Han
BACKGROUND: We previously reported that co-transplantation of exosomes from hypoxia-preconditioned adipose mesenchymal stem cells (ADSCs) improves the neoangiogenesis and survival of the grafted tissue. This study aimed to investigate the molecular mechanism of this protective effect. METHODS: Exosomes were collected from normoxia-treated (nADSC-Exo) or hypoxia--treated (hypADSC-Exo) human ADSCs, and their pro-angiogenic capacity was evaluated in human umbilical vein endothelial cells (HUVECs) and a nude mouse model of subcutaneous fat grafting...
January 30, 2019: International Journal of Biochemistry & Cell Biology
Zhi-Chao Wang, Fei-Zhou Huang, Hong-Bo Xu, Ji-Chun Sun, Chang-Fa Wang
PURPOSE: Autophagy play an important role in tumor chemotherapy resistance. It has been reported that miR-137 expression was reducedand involved in the regulation of sensitivity of PC cells to chemotherapy. However, little is known about the underlying molecular mechanisms. In this study, we hypothesized that miR-137 might sensitize PC cells to chemotherapy thought regulating cell autophagy. METHODS: Cell survival was determined with MTT assay. Apoptotic cells were assessed with flow cytometric analysis...
January 30, 2019: International Journal of Biochemistry & Cell Biology
Meifang Lin, Ting Lei, Ju Zheng, Shuqin Chen, Liu Du, Hongning Xie
Dysregulation of ubiquitin-conjugating enzyme E2S (UBE2S) contributes to tumor progression. However, its clinical significance and biological function in endometrial cancer (EMC) remain unclear. Here, we show that UBE2S is upregulated in EMC and exhibits oncogenic activities via activation of SOX6/β-Catenin signaling. High expression of UBE2S is significantly associated with poor prognosis in two independent cohorts consisting of a total of 773 patients with EMC. In vitro studies demonstrate that ectopic expression of UBE2S promotes cell proliferation and migration, whereas knockdown of UBE2S results in opposite phenotypes...
January 25, 2019: International Journal of Biochemistry & Cell Biology
Yu Xing, Ling Li
BACKGROUND: The in vivo protective role of gastrodin (GSTD) in myocardial infarction (MI) has been reported. However, the underlying mechanism remains unclear. Herein, we aimed to explore the effects of GSTD on hypoxia-injured H9c2 cells as well as the downstream miRNAs and signaling cascades. METHODS: Hypoxic injury model was constructed to mimic MI. Effects of GSTD pretreatment on cell proliferation and apoptosis were measured by CCK-8 assay, Western blot analysis, and flow cytometry/Western blot analysis, respectively...
January 23, 2019: International Journal of Biochemistry & Cell Biology
Biqiang Zhu, Lei Zhao, Yang Liu, Ye Jin, Jing Feng, Fuya Zhao, Jiayu Sun, Rui Geng, Yunwei Wei
Cholangiocarcinoma (CCA) is one of the most common fatal carcinomas and is well known to be lack of effective treatment. Thus, novel therapeutic strategies are greatly needed. Evodiamine, a quinozole alkaloid isolated from evodia rutaecarpa Bentham, has been demonstrated to exhibit anti-tumor effects on many cancer cells. However, little is known in terms of the effects on cholangiocarcinoma. In this study, we studied whether this traditional Chinese Medicine could serve as new potential therapeutic drugs to treat CCA...
January 22, 2019: International Journal of Biochemistry & Cell Biology
Jingyuan Zhao, Zibo Meng, Chao Xie, Chong Yang, Zhiqiang Liu, Shihong Wu, Bo Wang, Ping Fan, Xin Jin, Heshui Wu
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies worldwide. PDAC is resistant to chemotherapy and radiotherapy which leads to the poor prognosis of PDAC patients and a 5-year survival rate of less than 5%. Exploring the mechanism of the pancreatic cancer tumorigenesis is the key to finding a novel therapeutic strategy for cancer treatment. B7-H3 belongs to the B7 family of immunoregulatory proteins, and the overexpression of B7-H3 is found in various types of cancer. The regulation of B7-H3 expression in pancreatic cancer is still unclear...
January 18, 2019: International Journal of Biochemistry & Cell Biology
Enze Li, Yi Sun, Guixiang Lv, Yongning Li, Zhiguo Zhang, Zheng Hu, Wenwu Cao
Sonodynamic therapy (SDT) is a noninvasive therapeutic method via the activation of certain chemical sensitizers using low intensity ultrasound. In this work, we evaluated the antitumor effect of sinoporphyrin sodium (DVDMS) mediated SDT (DVDMS-SDT) on Hepatocellular carcinoma (HCC) cell lines both in vitro and in vivo. The results indicated that DVDMS-SDT was significantly more efficacious than PpIX-SDT in treating hepatocellular cell line Hep-G2. DVDMS-SDT also increased the ratio of cells in the G2/M phase and decreased the CDK1 and Cyclin B1 protein level...
January 17, 2019: International Journal of Biochemistry & Cell Biology
Soraya Emamgholizadeh Minaei, Samideh Khoei, Sepideh Khoee, Mohammad Reza Karimi
In the present study, Folic Acid (FA) ligand was used to functionalize Temozolomide-loaded Poly (ethylene Glycol)-Poly (Butylene Adipate)-Poly (ethylene Glycol)-coated magnetite nanoparticles (TMZ-SPION-PEG-PBA-PEG) for targeted chemotherapy of glioma cells. Four types of nanoparticles were synthesized with the hydrodynamic diameters of 24-49 nm. Using MTT, Prussian blue, and ICP-OES assays, the cytotoxicity effect and cellular uptake of nanoparticles were evaluated in C6 cancer cells and OLN-93 normal cells...
January 17, 2019: International Journal of Biochemistry & Cell Biology
Mahnaz Jahangiri Moez, Hassan Bjeije, Bahram M Soltani
MicroRNAs are classified as small non-coding RNAs that regulate gene expression mainly through targeting the 3'UTR region of mRNAs. A great number of miRNAs play important role in the regulation of signaling pathways in normal and cancer cells. Here, we predicted hsa-miR-5195-3p (miR-5195-3p) as a potential regulator of TGFβ signaling and investigated its effect on TGFB-R1, TGFB-R2, SMAD2, SMAD3 and SMAD4 transcripts which are key players of TGFβ/SMAD signaling pathway. Overexpression of miR-5195 in HCT116 cells resulted in a significant reduction of TGFB-R1, SMAD2, SMAD3, and SMAD4 at the mRNA level which was confirmed using RT-qPCR...
January 16, 2019: International Journal of Biochemistry & Cell Biology
Inês Baptista, Magda Santos, Alisa Rudnitskaya, Jorge A Saraiva, Adelaide Almeida, Sílvia M Rocha
Staphylococcal food poisoning is a disease that originates significant health and economic losses and is caused by Staphylococcus aureus strains able to produce enterotoxins. The aim of this work is to go further on the study of the volatile exometabolome of S. aureus using an advanced gas chromatographic technique. Enterotoxic and non-enterotoxic strains were assessed. The volatile exometabolome profile comprised 240 volatiles belonging to ten chemical families. This volatiles were mainly by-products of branched-chain amino acids and methionine degradation, pyruvate metabolism, diacetyl pathway, oxidative stress and carotenoid cleavage...
January 12, 2019: International Journal of Biochemistry & Cell Biology
Maria Skrzypkowska, Bartosz Słomiński, Monika Ryba-Stanisławowska, Urszula Ławrynowicz, Piotr Gutknecht, Janusz Siebert
BACKGROUND: CCR5 is a chemokine receptor expressed by various populations including leukocytes, smooth muscle cells and endothelium. Δ32 polymorphism of CCR5 gene has been connected with, inter alia, cardiovascular disease development. The aim of our study was to evaluate impact of CCR5 variant on CD34+ and CD34+VEGFR2+ cells - populations involved in cardiovascular system homeostasis and regeneration. METHODS AND RESULTS: We have examined 170 Polish subjects from Pomeranian region...
January 12, 2019: International Journal of Biochemistry & Cell Biology
Norbert Weiss, Gerald W Zamponi
T-type calcium channels serve an essential role in the functioning of the nervous system. They exhibit unique properties among voltage-gated calcium channels, and mediate specific roles in brain function. Over the recent years, it has come to light that a number of chronic neurological disorders arise from defects in T-type channel function. The question then arises as to whether T-type channels could represent a relevant druggable target. In this review, we introduce the diversity, molecular structure, and principal electrophysiological properties of T-type channels...
January 12, 2019: International Journal of Biochemistry & Cell Biology
Claudio de Lucia, Michela Piedepalumbo, Giuseppe Paolisso, Walter J Koch
Cardiovascular diseases such as heart failure and metabolic syndrome have high prevalence in the elderly population and are leading causes of death, disability, hospitalization, driving high healthcare costs worldwide. To reduce this social and economic burden there is urgency to find effective therapeutic targets. Several studies have linked the dysfunction of the Sympathetic Nervous System and β-adrenergic receptor signaling with the pathogenesis of age-related cardiovascular diseases. Therapeutic treatments that restore their functions have been shown to be effective in subjects with cardiovascular comorbidities...
January 9, 2019: International Journal of Biochemistry & Cell Biology
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