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Fish & Shellfish Immunology

Mehdi Naderi Farsani, Seyed Hossein Hoseinifar, Ghasem Rashidian, Hamed Ghafari Farsani, Ghasem Ashouri, Hien Van Doan
This investigation was aimed to determine the efficacy of coriander seed extract (Coriandrum sativum) on physiological responses, immunity and disease resistance of rainbow trout, Oncorhynchus mykiss for eight weeks. A total number of six hundred rainbow trout (62 ± 0.81 g) were divided into four feeding groups including 0 (control), 0.5%, 1% and 2% of coriander seed extract (CSE). In the present study, rainbow trout fed with 2% of CSE showed significantly higher values of specific growth rate (SGR), final weight (FW) and condition factor (CF) in comparison with control group after eight weeks (P < 0...
May 15, 2019: Fish & Shellfish Immunology
Chang Tan, Huihui Zhou, Xuan Wang, Kangsen Mai, Gen He
Adding immunopotentiators to plant protein based diets has been a feasible way to improve fish growth performance and healthy status. In this study, an 8-week trial was carried out to explore the effects of resveratrol, a natural polyphenolic compound, on growth performance, anti-oxidative capacity and immune responses in turbot fed soybean meal based diet. As the results showed, replacement 45% fish meal with soybean meal (SBM) significantly depressed the fish growth, feed utilization and the heights of villi and microvilli in distal intestine...
May 15, 2019: Fish & Shellfish Immunology
Cuiyun Zou, Minglei Xu, Leling Chen, Qingying Liu, Yuanyuan Zhou, Zhenzhu Sun, Huaqun Ye, Ningning Su, Chaoxia Ye, Anli Wang
Excessive lipid accumulation and chemical abuse can induce fatty liver diseases in fish, but the underlying mechanism and therapies are unknown. The present study aims to evaluate the effects of Xiaochaihu Decoction (XCHD) on the growth performance, lipid metabolism and antioxidant function of hybrid grouper in vitro and in vivo, and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. In vitro, steatosis model of hybrid grouper primary hepatocytes were incubated for 48 h in control or lipid emulsion (LE)-containing medium with or without 24 h post-treatment with XCHD...
May 14, 2019: Fish & Shellfish Immunology
K Muñoz, P Flores-Herrera, A T Gonçalves, C Rojas, C Yáñez, L Mercado, K Brokordt, P Schmitt
All organisms live in close association with a variety of microorganisms called microbiota. Furthermore, several studies support a fundamental role of the microbiota on the host health and homeostasis. In this context, the aim of this work was to determine the structure and diversity of the microbiota associated with the scallop Argopecten purpuratus, and to assess changes in community composition and diversity during the host immune response. To do this, adult scallops were immune challenged and sampled after 24 and 48 h...
May 14, 2019: Fish & Shellfish Immunology
Qiao Ye, Yongyong Feng, Zhenlu Wang, Aiguo Zhou, Shaolin Xie, Yue Zhang, Qiong Xiang, Enfeng Song, Jixing Zou
The present study aim to investigate the effects of dietary Gelsemium elegans alkaloids supplementation in Megalobrama amblycephala. A basal diet supplemented with 0, 5, 10, 20 and 40 mg/kg G. elegans alkaloids were fed to M. amblycephala for 12 weeks. The study indicated that dietary 20 mg/kg and 40 mg/kg G. elegans alkaloids supplementation could significantly improve final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) (P < 0...
May 14, 2019: Fish & Shellfish Immunology
Sajal Kole, Syed Shariq Nazir Qadiri, Su-Mi Shin, Wi-Sik Kim, Jehee Lee, Sung-Ju Jung
Viral haemorrhagic septicaemia virus (VHSV), a (-) ssRNA virus belonging to the genus Novirhabdovirus of rhabdoviridae family, is the aetiological agent of viral haemorrhagic septicaemia (VHS) disease which causes huge economic losses in farmed olive flounder (Paralichthys olivaceus) and significant mortalities among several other marine fish species in Korea, Japan, and China. Previously, we developed an inactivated vaccine viz., formalin-inactivated VHSV mixed with squalene as adjuvant which was effective in conferring protective immunity (58-76% relative percentage survival) against VHSV but the mode of administration was intraperitoneal injection which is not feasible for small sized fingerling fish...
May 13, 2019: Fish & Shellfish Immunology
Liangliang Mu, Xiaoxue Yin, Hairong Wu, Kailiang Han, Liting Wu, Mingmei Ding, Xia Bian, Bingxi Li, Shengli Fu, Fang Liang, Zheng Guo, Jianmin Ye
Mannose-binding lectin-associated serine protease-1 (MASP-1), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system. In this study, a MASP-1 homolog (OnMASP-1) was identified from Nile tilapia (Oreochromis niloticus) and characterized at expression and inflammation functional levels. The open reading frame (ORF) of OnMASP-1 is 2187 bp of nucleotide sequence encoding a polypeptide of 728 amino acids...
May 13, 2019: Fish & Shellfish Immunology
Ying Huang, Ruidong Zhang, Tianheng Gao, Hui Xu, Ting Wu, Qian Ren
As a type of pattern-recognition proteins (PRRs), C-type lectins (CTLs) perform important functions in non-self recognition and clearance of pathogens in innate immunity. In this study, a unique 2-transmembrane CTL (designated as Mn-2TM-cLec) with a single carbohydrate recognition domain (CRD) was isolated from Macrobrachium nipponense. The full-length cDNA of Mn-2TM-cLec consisted of 3265 bp with an 837 bp open reading frame encoding a protein with 278 amino acids. Mn-2TM-cLec was ubiquitously distributed in various tissues of normal prawn, particularly in the hemocytes, hepatopancreas, and gills...
May 13, 2019: Fish & Shellfish Immunology
W M Gayashani Sandamalika, Thanthrige Thiunuwan Priyathilaka, Seongdo Lee, Hyerim Yang, Jehee Lee
Representing a multifunctional complex group of proteins, glutathione S- transferases (GSTs) play a major role in the phase II detoxification process in a wide range of organisms. This study focused on the potential detoxification ability of disk abalone (Haliotis discus discus) GST theta (AbGST-θ) under different stress conditions with special reference to post immune challenges. Characterization of AbGST-θ revealed with 226 amino acids, 26.6 kDa of predicted molecular mass and 8.9 of theoretical isoelectric point...
May 12, 2019: Fish & Shellfish Immunology
Peng Fei Zou, Juan Juan Shen, Ying Li, Qingpi Yan, Zhi Hua Zou, Zi Ping Zhang, Yi Lei Wang
As an adaptor in Toll-like receptor (TLR) signaling pathway, Toll/interleukin-1 receptor (TIR) domain containing adaptor inducing interferon-β (TRIF) mediates downstream signaling cascades and plays important roles in host innate immune responses. In the present study, a TRIF ortholog named Lc-TRIF was identified in large yellow croaker (Larimichthys crocea). Sequence comparison analysis revealed that Lc-TRIF has a conserved TIR domain but without TRAF6 binding motif. The genome structure of Lc-TRIF is conserved, with two exons and one intron...
May 12, 2019: Fish & Shellfish Immunology
R Jenabi Haghparast, K Sarvi Moghanlou, M Mohseni, A Imani
A feeding experiment was conducted to evaluate the effects of dietary soybean lecithin (SBL) supplementation on performance, hemato-immunological parameters, lipid biochemistry, antioxidant status, digestive enzymes activity and intestinal histomorphometry of Caspian brown trout, Salmo trutta caspius in the pre-spawning stage. The basal diet was supplemented with 0% (control), 3%, 6%, 9% and 12% of SBL to obtain five experimental diets. Fish with an average weight of 350 ± 10 g were randomly distributed among five experimental groups and fed for 90 days...
May 11, 2019: Fish & Shellfish Immunology
Mohammad Javad Jami, Abdolmohammad Abedian Kenari, Hamed Paknejad, Mahmoud Mohseni
This study investigated the effect of individual and combination of dietary pre- and probiotics (β-glucan, 3 mg/g; mannan oligosaccharide (MOS), 4 mg/g; and Lactobacillus plantarum; 108  CFU/mg diet) on growth performance, blood immune parameters, expression of immune related genes, and intestinal microbial of Caspian trout (Salmo trutta caspius). On the basis of feeding with immunostimulant diets, the fish were assigned into eight groups denoted as: control (basal diet), bβ (basal diet + β-glucan), bM (basal diet + MOS), bLp (basal diet + L...
May 11, 2019: Fish & Shellfish Immunology
Soibam Khogen Singh, V K Tiwari, N K Chadha, Sukham Munilkumar, Chandra Prakash, Nilesh A Pawar
In this study, we tried to investigate the effect of dietary Bacillus circulans PB7 (BCPB7) and fructoligosaccharide (FOS), singly or in combination for evaluation of growth, immune and physiological status of Labeo rohita (rohu) juveniles reared under low pH and normal pH for 60 days. Experimental fishes were distributed in two sets such as one set continuously exposed to low pH (5.5) and other reared under normal pH (7.0), and fed with four iso-nitrogenous diets viz. basal (control), Bacillus circulans PB7 (BCPB7, 106 cfug-1 ), 1% fructooligosaccharide (FOS) and their combination...
May 11, 2019: Fish & Shellfish Immunology
Shengli Fu, Mingmei Ding, Qingjian Liang, Yanjian Yang, Meng Chen, Xiufang Wei, Anli Wang, Shaoan Liao, Jianmin Ye
The immune mechanism elicited in pufferfish (Takifugu obscurus) against the invasion of Aeromonas hydrophila is still poorly understood. We examined the spleen of pufferfish at the transcriptome and proteome levels by using Illumina-seq and TMT coupled mass spectrometry after 12 h infection by A. hydrophila, respectively. A total of 2,339 genes (1,512 up-regulated and 827 down-regulated) and 537 (237 up-regulated and 300 down-regulated) proteins were identified. GO and KEGG analyses revealed that the responses to stimulus were the main biological processes, intestinal immune network for IgT production and calcium signaling pathway...
May 11, 2019: Fish & Shellfish Immunology
Saekil Yun, Sib Sankar Giri, Hyoun Joong Kim, Sang Geun Kim, Sang Wha Kim, Jung Woo Kang, Se Jin Han, Jun Kwon, Woo Taek Oh, Cheng Chi, Jin Woo Jun, Se Chang Park
Immunization by bath immersion is likely the simplest method of fish vaccination. Although the route of immunogenicity has not been fully identified, immersion vaccination is clearly a useful labor-saving technique. In this study, microbubble (MB) treatment was assessed for its ability to improve the efficacy of bath immersion vaccination in the cyprinid loach. MBs are commonly defined as minute particles of gas with a diameter of less than 100 μm, which generated free radicals. Here, the efficacy of MB treatment for vaccination enhancement in the cyprinid loach was assessed in direct challenge experiments using the virulent Aeromonas hydrophila JUNAH strain; assessments comprised agglutination titer assay and non-specific parameter analysis...
May 11, 2019: Fish & Shellfish Immunology
Fang Han, Wanbo Li, Xiande Liu, Dongling Zhang, Lanping Liu, Zhiyong Wang
The Rho family GTPase Rac1 acts as a molecular switch for signal transduction to regulate various cellular functions. Here, a Rac1 homolog (LcRac1) was identified in large yellow croaker (Larimichthys crocea), one of the most economically important marine fishes. The LcRac1 protein was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-LcRac1). LcRac1 was ubiquitously expressed in all 12 tissues we examined, especially it was highest expression in heart and blood and the weakest expression in head-kidney and spleen...
May 10, 2019: Fish & Shellfish Immunology
Jiaxin Liu, Youhua Huang, Xiaohong Huang, Chen Li, Song Wei Ni, Yepin Yu, Qiwei Qin
DEAD (Asp-Glu-Ala-Asp)-box polypeptide 41 (DDX41) is a member of the DEXDc family of helicases, that has recently been identified to be a crucial intracellular DNA sensor that triggers multiple signaling molecules to activate the type I interferon response. However, the precise function of DDX41 in fish during a viral infection remains unknown. In the present study, the DDX41 homolog from orange spotted grouper, Epinephelus coioides (EcDDX41), was cloned and its potential role in the immune response to a fish viral infection were investigated...
May 10, 2019: Fish & Shellfish Immunology
Jinshu Guo, Panhong Wu, Jinling Cao, Yongju Luo, Jianjie Chen, Guodong Wang, Wenjing Guo, Tianyu Wang, Xinjing He
Excessive perfluorooctane sulfonate (PFOS) in natural water ecosystem has the potential to detrimentally affect immune system, but little is known of such effects or underlying mechanisms in fish. In the present study, we evaluated the effects of PFOS on growth performance, organizational microstructure, activities of immune related-enzymes and expressions of immune related-genes in male zebrafish (Danio rerio) exposed to different concentrations of 0, 0.02, 0.04 and 0.08 mg/L of PFOS for 7, 14, and 21 days to investigate the effects of PFOS on immune system and the potential toxic mechanisms caused by PFOS...
May 10, 2019: Fish & Shellfish Immunology
Mostafa Halimi, Mojtaba Alishahi, Mohammad Reza Abbaspour, Masoud Ghorbanpoor, Mohammad Reza Tabandeh
The effectiveness of ionotropic gelation method (by combining alginate and chitosan) vaccine against Lactococcus garvieae and Streptococcus iniae was examined in rainbow trout. Fish were separated into four groups and fed the distinctive examined feeds. Our groups were included: A) fish immunized by chitosan-alginate coated vaccine, B) fish immunized by non-coated vaccine, C) fish feed by chitosan-alginate coated pellets without vaccine and D) fish feed by basic diet (non-coated and without vaccine). In groups A and B, the vaccination was carried out for 14 days...
May 10, 2019: Fish & Shellfish Immunology
Wanying Yang, Wenxiu Liu, Chungen Wen, Baoqing Hu, Shaoqing Jian, Yang Gang
Manganese superoxide dismutase (MnSOD) is a sort of important metalloenzyme that can catalyze ROS in the organisms. In this study, MnSOD cDNA of C. plicata, designated as CpMnSOD (accession no. MK465057), was cloned from hemocytes. The full-length cDNA of MnSOD was 1096 bp with a 672 bp open reading frame encoding 223 amino acids. The deduced amino acid sequence contained a mitochondrial-targeting sequence (MTS) of 18 amino acids in the N-terminus, and four conserved amino acids for manganese binding (H49 , H97 , D182 , H186 )...
May 9, 2019: Fish & Shellfish Immunology
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