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Protein and Peptide Letters

Kamal U Zaidi, Firoz Naem Khan, Sharique A Ali, Kausar Parveen Khan
Melanin plays a crucial role in camouflage, social communication and protection against harmful ultraviolet radiations. Melanin is a synthesized by melanocytes through melanogenesis and several intrinsic and extrinsic factors are involved during the process. Any change occur in normal melanogenesis process can cause severe pigmentation problems of hypopigmentation or hyperpigmentation. The present study is based on evaluation of the effect of thymoquinone on melanogenesis and their possible mechanism of action using B16F10 melanoma cell line for the production via blocking signaling pathways...
May 6, 2019: Protein and Peptide Letters
Farheen Naz, Haider Anis, Ziaul Hasan, Asimul I Slam, Luqman A Khan
Fungal lipase dependent processes are important virulence attributes. We explore Aspergillus niger lipase as a direct target of eugenol through spectroscopic techniques and compare results to Bovine Serum Albumin and lysozyme to comment on selectivity of eugenol towards lipase. Eugenol IC50 of lipase was 150 μg/ml. UV-vis spectroscopy shows formation of lipase-eugenol, Bovine Serum Albumin-eugenol and lysozyme-eugenol complex at concentrations well below IC50. Eugenol binding caused blue shift with Bovine Serum Albumin and lysozyme suggestive of compaction, and red shift with lipase...
May 6, 2019: Protein and Peptide Letters
Yijie Ding, Jijun Tang, Fei Guo
The identification of Drug-Target Interactions (DTIs) is an important process in drug discovery and medicine research. However, the tradition experimental methods for DTIs identification are still time consuming, extremely expensive and challenging, even now. In the past ten years, various computational methods have been developed to identify potential DTIs. In this paper, the identification methods of DTIs are summarized. What's more, several state-of-the-art computational methods are mainly introduced, containing network-based method and machine learning-based method...
April 10, 2019: Protein and Peptide Letters
Wei Chen, Fulei Nie, Hui Ding
Phage virion proteins (PVP) are essential materials of bacteriophage, which participate in a series of biological processes. Accurate identification of phage virion proteins is helpful to understand the mechanism of interaction between the phage and its host bacteria. Since experimental method is labor intensive and time-consuming, in the past few years, many computational approaches have been proposed to identify phage virion proteins. In order to facilitate researchers to select appropriate methods, it is necessary to give a comprehensive review and comparison on existing computational methods on identifying phage virion proteins...
April 10, 2019: Protein and Peptide Letters
Norio Matsushima, Hiroki Miyashita, Shinsuke Tamaki, Robert H Kretsinger
Plant peptide hormones play a crucial role in plant growth and development. A group of these peptide hormones are signaling peptides with 5 ⎼ 23 amino acids. Flagellin peptide (flg22) also elicits an immune response in plants. The functions are expressed through recognition of the peptide hormones and flg22. This recognition relies on membrane localized receptor kinases with extracellular leucine rich repeats (LRR⎼RKs). The structures of plant peptide hormones ⎼ AtPep1, IDA, IDL1, RGFs 1⎼ 3, TDIF/CLE41 ⎼ and of flg22 complexed with LRR domains of corresponding LRR⎼RKs and co-receptors SERKs have been determined...
April 8, 2019: Protein and Peptide Letters
Deborah L Heyl, Brandon Iwaniec, Daniel Esckilsen, Deanna Price, Prathyusha Guttikonda, Jennifer Cooper, Julia Lombardi, Maria Milletti, Hedeel Guy Evans
Amyloid fibrils in Alzheimer's disease are composed of amyloid-β (Aβ) peptides of variant lengths. Humanin (HN), a 24 amino acid residue neuroprotective peptide, is known to interact with the predominant Aβ isoform in the brain, Aβ (1-40). Here, we constructed smaller segments of Aβ and HN and identified residues in HN important for both HN-HN and HN-Aβ interactions. Peptides corresponding to amino acid residues 5-15 of HN, HN (5-15), HN (5-15, L11S), where Leu11 was replaced with Ser, and residues 17-28 of Aβ, Aβ (17-28), were synthesized and tested for their ability to block formation of the complex between HN and Aβ (1-40)...
April 5, 2019: Protein and Peptide Letters
Shan Shan Hao, Man Man Zong, Ze Zhang, Jia Xi Cai, Yang Zheng, Xiu Li Feng, Chen Wang
Back: Bursa of Fabricius is the acknowledged central humoral immune organ. The bursal-derived peptides play the important roles on the immature B cell development and antibody production. OBJECTIVES: Here we explored the functions of the new isolated bursal hexapeptide and pentapeptide on the humoral, cellular immune response and antigen presentation to avian influenza virus ( AIV) vaccine in mice immunization. METHOD: The bursa extract samples were purified following RP HPLC method, and were analyzed with MS/MS to identify the amino acid sequences...
April 5, 2019: Protein and Peptide Letters
Thanh-Hoa Nguyen-Thi, Kien-Quang Huynh, Phuong-Linh Dinh-Thi, Linh-Thuoc Tran, Yong Suk Jang, Hieu Tran-Van
The number of oral vaccines is still limited due to many difficulties suffered in the intestinal environment, such as mucosal clearance, vast area, harsh conditions, deteriorative enzymes, impermeability, tolerance, etc. Numerous strategies have focused on directing antigen to the receptors of M cells, which is the main gateway to acquire and initiate specific responses to antigens in intestine. FimHrb is a receptor binding domain of type 1 of fimbriae from E. coli and Salmonella that can bind to GP2 receptor expressed exclusively on M cells...
April 5, 2019: Protein and Peptide Letters
Cadena-Cadena Francisco, Cárdenas-López José Luis, Ezquerra-Brauer Josafat Marina, Cinco-Moroyoqui Francisco Javier, López-Zavala Alonso Alexis, Santacruz-Ortega Hisila Del Carmen, Rivero-Espejel Ignacio Alfredo
Cathepsin D of the Jumbo squid (Dosidicus gigas) hepatopancreas was purified and partially characterized. The secondary structure of these enzymes is highly conserved so the role of temperature and pH in the secondary structure and in protein denaturation is of great importance in the study of enzymes. The secondary structure of cathepsin D from jumbo squid hepatopancreas was determined by means of circular dichroism spectroscopy. It was found that α helix decreases at temperatures above 50 °C and above pH 4...
April 5, 2019: Protein and Peptide Letters
Syed Sayeed Ahmad, Mohammad A Kamal
The most recent decade was described by a developing awareness about the seriousness of dementia in the field of age-related people. Among the dementias, Alzheimer's assumes a generous role as a result of its amazingly high rate and casualty. A few pharmacological procedures have been attempted yet at the same time now, Alzheimer continues being an untreatable malady. The collection of Aβ in the brain is an early poisonous occasion in the pathogenesis of Alzheimer's disease, which is the most widely recognized type of dementia correlated with plaques and tangles within the brain...
April 5, 2019: Protein and Peptide Letters
Xian Li, Long Xia, Xiaohui Ouyang, Qimuge Suyila, Liya Su, Xiulan Su
BACKGROUND: Despite new agent development and short-term benefits in patients with colorectal cancer (CRC), metastatic CRC cure rates have not improved due to high rates of oxaliplatin resistance and toxicity. There is an urgent need for effective tools to prevent and treat CRC and reduce morbidity and mortality of CRC patients. Exploring the effects of bioactive peptides on the antitumor to CRC was of vital importance to the clinical application. OBJECTIVE: This study aimed to investigate the therapeutic impact of anticancer bioactive peptides (ACBP) on anticancer effect of oxaliplatin (LOHP) in human colorectal cancer xenografts models in nude mice...
April 5, 2019: Protein and Peptide Letters
Lucas Miranda Kangussu, Tatiane Cristine S de Almeida, Thiago Ruiz R Prestes, Marilda Luz de Andrade De Maria, Roberta da Silva Filha, Maria Aparecida Ribeiro Vieira, Ana Cristina Simões E Silva, Anderson José Ferreira
BACKGROUND: Angiotensin converting enzyme (ACE) 2 is an important modulator of the renin angiotensin system (RAS) and the RAS plays a central role in renovascular hypertension. Very few studies investigated the role of components of the counterregulatory RAS axis (ACE2, Ang-(1-7) and Mas receptor) in renovascular hypertension and the results are controversial. OBJECTIVE: The aim of this study was to investigate the effects of diminazene aceturate (DIZE) administration on renal function and renal inflammation parameters in 2K1C hypertensive rats...
April 5, 2019: Protein and Peptide Letters
A R Satvik Iyengar, Abhay H Pande
Nerve agents have been used extensively in chemical warfare in the past. However, recent use of Novichok agents have reignited the debate on the threat posed by Organophosphorus Nerve Agents (OPNAs). The currently available therapy for OPNA toxicity is only symptomatic and is potentially ineffective in neutralizing OPNAs. Hence, there is a dire need to develop a prophylactic therapy for counteracting OPNA toxicity. In this regard, human paraoxonase 1 is the enzyme of choice. In this review, we have focussed upon the recent and past events of OPNA use, their mechanism of action and toxicity...
April 3, 2019: Protein and Peptide Letters
Jia Liu, Ping Song, Jie Zhang, Ziyan Nangong, Xiaobei Liu, Yue Gao, Qinying Wang
Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene encoding chitin binding protein (Xn-CBP). In order to study the characteristics and function of Xn-CBP, the 600 bp Xn-cbp gene was cloned and sequenced from X. nematophila HB310. Sequence analyses showed that the Xn-cbp gene encoded 199 amino acids with a calculated mass of 28 kDa and an isoelectric point of 8.00. Xn-CBP contained a signal peptide and a chitin binding domain. Xn-CBP was expressed with histidine tags in Escherichia coli BL21 (DE3) and purified...
March 27, 2019: Protein and Peptide Letters
Sameer Suresh Bhagyawant, Dakshita Tanaji Narvekar, Neha Gupta, Amita Bhadkaria, Ajay Kumar Gautam, Nidhi Srivastava
BACKGROUND: Diabetes and hypertension are the major health concern and is alleged to be of epidemic proportions. This has made it a numero uno subject at various levels of investigation. Glucosidase inhibitor provides the reasonable option in treatment of diabetes mellitus (DM) as it specifically target post prandial hyperglycemia. The angiotensin converting enzyme (ACE) plays an important role in hypertension. Therefore, inhibition of ACE in treatment of elevated blood pressure attracts special interest of the scientific community...
March 27, 2019: Protein and Peptide Letters
Krishna Kant, Anil Kumar Tomar, Pankaj Sharma, Bishwajit Kundu, Sarman Singh, Savita Yadav
BACKGROUND: A well-known tissue marker of ovarian cancer, human epididymis protein 4 (HE4) is the member of whey acidic four-disulfide core proteins family. Purified from human seminal plasma and characterized as a cross-class protease inhibitor, HE4 was proposed to shield spermatozoa against proteolytic factors. However, its exact biological function is unknown. Proteins usually function in conjunction with other proteins in the system and thus, identification and analysis of protein networks become essential to decode protein functions...
March 27, 2019: Protein and Peptide Letters
Priyanka Biswas, Dillip Kumar Sahu, Kalyanasis Sahu, Rajat Banerjee
Aminoacyl-tRNA synthetases (aaRSs) play an important role in catalyzing the first step in protein synthesis. Asparaginyl-tRNA synthetase (AsnRS) from Brugia malayi, Leishmania major, Thermus thermophilus, Trypanosoma brucei have been shown to play an important role in survival and pathogenesis. Entamoeba histolytica (Ehis) is an anaerobic pathogen that infects humans. Ehis-AsnRS has not been studied in detail, except the crystal structure determined at 3 Å resolution showing that it is primarily α-helical and dimeric...
March 27, 2019: Protein and Peptide Letters
Mohammed Auwal Ibrahim, June C Serem, Megan J Bester, Albert W Neitz, Anabella R M Gaspar
BACKGROUND: Peptide-based therapeutics offer a unique avenue for the development of novel agents for the treatment of diabetes mellitus including α-glucosidase inhibitors. The peptide, SQSPA, was reported to possess to α-glucosidase inhibitory activity in addition to resistance to the gastrointestinal tract (GIT) digestion. METHOD: In this study, the in silico and in vitro structure-activity analyses of the peptide was conducted using alanine scanning to identify key amino acid residues...
March 27, 2019: Protein and Peptide Letters
Ting Song, Keke Cao, Yudan Fan, Zhichao Zhang, Zongwei Guo, Minhang Zhang, Peng Liu
The significance of multi-site phosphorylation of BCL-2 protein in the flexible loop domain remains controversial, in part due to the lack of structural biology studies of phosphorylated BCL-2. We constructed a phosphomietic mutant BCL-2(62-206) (t69e, s70e and s87e) (EEE-BCL-2-EK(62-206)), in which the BH4 domain and the part of loop region was trunkated (residues 2-61). In this way,we obtained a mass of soluble and stable phosphomimetic proteins to produce a well dispersed 15N-1H heteronuclear single quantum coherence (HSQC) NMR spectroscopy...
March 27, 2019: Protein and Peptide Letters
Nousheen Bibi, Hafsa Niaz, Ted Hupp, Mohammad Amjad Kamal, Sajid Rashid
Human proteome contains a plethora of short linear peptide motifs that is crucial for signaling and other cellular processes. These motifs are difficult to identify due to lack of systematic approach for their detection. Here we demonstrate the use of peptide phage display in combination with high throughput next generation sequencing to identify enriched peptide sequences through biopanning process against polo box domain (PBD) of mitotic polo like kinase 1 (Plk1). Purified recombinant Plk1 and two unrelated controls namely B-lymphocyte antigen (CD20) and fluorescent protein (mCherry) were subjected to peptide phage display analysis...
March 17, 2019: Protein and Peptide Letters
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