Viviane Dal-Souto Frescura, Haywood Dail Laughinghouse, Thais Scotti do Canto-Dorow, Solange Bosio Tedesco
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed...
December 2012: Biocell: Official Journal of the Sociedades Latinoamericanas de Microscopía Electronica ... Et. Al