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Microbiological Research

Linda Höll, Maik Hilgarth, Andreas J Geissler, Jürgen Behr, Rudi F Vogel
Modified atmosphere packaging (MAP) is widely used in food industry to extend the microbiological shelf life of meat. Common CO2 -containing gas atmospheres for poultry meat packaging are either nearly O2 -free or high O2 MAPs. In this work, we compared spoilage microbiota of skinless chicken breast in CO2 /O2 (30/70%) and CO2 /N2 (30/70%) MAP, which are culturable with conventional methods and identified isolates by MALDI-TOF MS. These data were compared to metatranscriptome sequencing enabling a culture-independent overview on the composition of microbiota at species level...
May 2019: Microbiological Research
Lingzhi Li, Hongmei Meng, Dan Gu, Yang Li, Mengdie Jia
Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is mainly distributed in the seafood such as fish, shrimps and shellfish throughout the world. V. parahaemolyticus can cause diseases in marine aquaculture, leading to huge economic losses to the aquaculture industry. More importantly, it is also the leading cause of seafood-borne diarrheal disease in humans worldwide. With the development of animal model, next-generation sequencing as well as biochemical and cell biological technologies, deeper understanding of the virulence factors and pathogenic mechanisms of V...
May 2019: Microbiological Research
Emine Seydametova, Jiwon Yu, Jonghyeok Shin, Yoonjung Park, Chakhee Kim, Hooyeon Kim, Seok Hyeon Yu, Yongcheol Park, Dae-Hyuk Kweon
2'-Fucosyllactose (2'-FL) is the most abundant human milk oligosaccharide and is important for infant nutrition and health. Because 2'-FL has potential as a functional ingredient in advanced infant formula and as a prebiotic in various foods, a cost-effective method for 2'-FL production is desirable. α1,2-Fucosyltransferase (α1,2-FT) is one of the key enzymes enabling the microbial biosynthesis of this complex sugar. However, the α1,2-FTs reported so far for the whole-cell biosynthesis of 2'-FL originate from pathogens, posing a potential hurdle for approval as a food production method depending on countries...
May 2019: Microbiological Research
Malathi Bheri, Sheetal M Bhosle, Ragiba Makandar
E. pisi is an ascomycete member causing powdery mildew disease of garden pea. It is a biotrophic pathogen, requiring a living host for its survival. Our understanding of molecular mechanisms underlying its pathogenesis is limited. The identification of proteins expressed in the pathogen is required to gain an insight into the functional mechanisms of an obligate biotrophic fungal pathogen. In this study, the proteome of the anamorphic stage of E. pisi pathogen has been elucidated through the nano LC-MS/MS approach...
May 2019: Microbiological Research
Sasimaporn Khemthong, Puttamas Nuonming, Sumontha Nookabkaewb, Rojana Sukchawalit, Skorn Mongkolsuk
Analysis of the Agrobacterium tumefaciens C58 genome revealed a potential Zur (zinc uptake regulator) binding site (5'-GATATGTTATTACATTAC-3', the underlined letters are the center of symmetry of the inverted palindrome) located in the upstream region of atu3184, whose gene product is a member of the COG0523 subfamily of G3E GTPases. The specific interaction of the Zur protein with the 18-bp inverted repeat operator motif in the presence of zinc was demonstrated in vitro by a DNA band shift assay and a DNase I footprinting assay...
May 2019: Microbiological Research
Kavleen Kaur, Aarjoo Sharma, Neena Capalash, Prince Sharma
The acquisition of metal ions such as iron, copper and manganese is essential for the survival of microorganisms as these are constituents of metalloproteins including enzymes, storage proteins, structural elements, transcription factors and antimicrobial factors in various biological processes. However, excess of these metal ions is associated with significant toxicity due to spontaneous redox cycling of ions and obstruction of normal metabolic pathways. To overcome this, microbes have developed a variety of metal regulatory systems allowing them to adapt to the changing biotic and abiotic environments...
May 2019: Microbiological Research
Lifan Wei, Haoxian Qiao, Bing Liu, Kaiyu Yina, Qin Liua, Yuanxing Zhang, Yue Ma, Qiyao Wang
No abstract text is available yet for this article.
April 2019: Microbiological Research
Bruna Agrillo, Sara Mirino, Rosarita Tatè, Lorena Gratino, Marta Gogliettino, Ennio Cocca, Nassira Tabli, Elhafid Nabti, Gianna Palmieri
Bacteria isolated from different environments can be exploited for biocontrol purposes by the identification of the molecules involved in the antifungal activity. The present study was aimed at investigating antifungal protein compounds purified from a previously identified plant growth promoting bacterium, Pseudomonas protegens N isolated from agricultural land in northern Algeria. Therefore, a novel protein was purified by chromatographic and ultrafiltration steps and its antifungal activity together with growth-inhibition mechanism was evaluated against different fungi by plate-based assays...
April 2019: Microbiological Research
Meng-Jun Tang, Qiang Zhu, Feng-Min Zhang, Wei Zhang, Jie Yuan, Kai Sun, Fang-Ji Xu, Chuan-Chao Dai
The endophytic fungus Phomopsis liquidambari play a key role in habitat adaptation of rice (Oryza sativa L.) with potential multiple beneficial. However, our previous published work on this subject remains incomplete. Here, we performed a soil nutrient (nitrogen and phosphorus) transformation with related functional genes and elucidated how rhizosphere microbiota vary their response to P. liquidambari interaction throughout the plant's life cycle under field conditions by Illumina Miseq sequencing platforms in a nutrient-limited paddy soil...
April 2019: Microbiological Research
Imran Afzal, Zabta Khan Shinwari, Shomaila Sikandar, Shaheen Shahzad
Endophytic bacteria are the plant beneficial bacteria that thrive inside plants and can improve plant growth under normal and challenging conditions. They can benefit host plants directly by improving plant nutrient uptake and by modulating growth and stress related phytohormones. Indirectly, endophytic bacteria can improve plant health by targeting pests and pathogens with antibiotics, hydrolytic enzymes, nutrient limitation, and by priming plant defenses. To confer these benefits, the bacteria have to colonize the plant endosphere after colonizing the rhizosphere...
April 2019: Microbiological Research
Lorena C Pena, Gustavo H Jungklaus, Daiani C Savi, Lisandra Ferreira-Maba, André Servienski, Beatriz H L N S Maia, Vinicius Annies, Lygia V Galli-Terasawa, Chirlei Glienke, Vanessa Kava
Endophytic fungi belonging to Muscodor genus are considered as promising alternatives to be used in biological control due to the production of volatile organic compounds (VOCs). The strains LGMF1255 and LGMF1256 were isolated from the medicinal plant Schinus terebinthifolius and, by morphological data and phylogenetic analysis, identified as belonging to Muscodor genus. Phylogenetic analysis suggests that strain LGMF1256 is a new species, which is herein introduced as Muscodor brasiliensis sp. nov. The analysis of VOCs production revealed that compounds phenylethyl alcohol, α-curcumene, and E (β) farnesene until now has been reported only from M...
April 2019: Microbiological Research
Amandeep Kaur, Neena Capalash, Prince Sharma
Quorum sensing plays important roles in the regulation of physiological and virulence processes in most bacteria, but its role in extremophiles is largely unknown. Comparative genomic, phylogenic, structural and signaling pathways analyses and deletion mutant studies have suggested the presence of three major quorum sensing systems (AI-1, Peptide based and AI-2) in extremophiles. Autoinducer-1(AI-1) system was found to be most prevalent (except in thermophiles where it is autoinducer-2) while peptide based system was least prevalent in extremophiles...
April 2019: Microbiological Research
Rosella Muresu, Andrea Porceddu, Leonardo Sulas, Andrea Squartini
The culturable bacteria from root nodules of Sulla coronaria growing in spontaneous conditions in Sardinia were characterized. This plant's peculiarity is to represent a legume still found in both wild and cropped statuses. We tested whether culturable bacteria would differ from those commonly isolated from its field-cropped varieties, to date exclusively represented by Rhizobium sullae. 63 isolates from 60 surface-sterilized nodules were analyzed by ARDRA and 16S rDNA sequencing. The official nitrogen-fixing symbiont Rhizobium sullae was found only in 25 nodules out of 60...
April 2019: Microbiological Research
Lara Eisenbach, Andreas J Geissler, Matthias A Ehrmann, Rudi F Vogel
Strains of Lactobacillus sakei can be isolated from a variety of sources including meat, fermented sausages, sake, sourdough, sauerkraut or kimchi. Selected strains are widely used as starter cultures for sausage fermentation. Recently we have demonstrated that control about the lactic microbiota in fermenting sausages is achieved rather by pairs or strain sets than by single strains. In this work we characterized the pan genome of L. sakei to enable exploitation of the genomic diversity of L. sakei for the establishment of assertive starter strain sets...
April 2019: Microbiological Research
Davide Pacifico, Paolo Margaria, Luciana Galetto, Mauro Legovich, Simona Abbà, Flavio Veratti, Cristina Marzachì, Sabrina Palmano
The biological bases of recovery of two grapevine cultivars, Nebbiolo and Barbera, showing different susceptibility and recovery ability to "flavescence dorée" (FD) phytoplasma infection were investigated. The expression over one vegetative season, in FD-recovered and healthy grapevines, of 18 genes involved in defence, hydrogen peroxide and hormone production was verified at two time points. Difference (Δ) between the relative expressions of August and July were calculated for each target gene of both cultivars...
March 2019: Microbiological Research
S Rafia, S Saran
Sestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukaryote where starvation stress initiates multicellular development. The single DdSesn-like gene was expressed and its endogenous functions were characterized. Both, the knockout and constitutively expressing strains were made and their involvement in starvation-induced autophagy was analyzed...
March 2019: Microbiological Research
Chi-Hung Lin, Robyn A Peterson, Audrey Gueniche, Ségolène Adam de Beaumais, Virginie Hourblin, Lionel Breton, Maria Dalko, Nicolle H Packer
Sweat is a secretory fluid that can be a source of unpleasant body odour due to interaction of resident bacteria with sweat components. Identification of glycoproteins in sweat suggests that protein-conjugated glycans may act as binding epitopes for bacteria, as found in other secretory fluids such as human milk, tears and saliva which help to protect epithelial surfaces from infection. We conducted proteomic and glycomic analysis of sweat to reveal an abundance of glycoproteins, predominantly carrying bi-antennary sialylated N-glycans with or without fucose...
March 2019: Microbiological Research
Astha Sinha, Sajitha Lulu, Vino S, W Jabez Osborne
Contamination of soil by textile effluent is a major threat found worldwide. These pollutants have diverse range of negative effects on the ecosystem, therefore restoration through cost effective biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) using phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile effluent polluted soil and was assessed for its plant growth promoting traits (PGP) and the PGP functional genes were amplified...
March 2019: Microbiological Research
Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao Wang
The type VI secretion system (T6SS) is a versatile molecular machinery widely distributed in Gram-negative bacteria. The activity of the T6SS is tightly regulated by various mechanisms, including quorum sensing (QS), iron concentration, and transcriptional regulators. Here we demonstrated that the stringent response regulator, RelA, contributes to bacterial resistance to multiple environmental stresses in Yersinia pseudotuberculosis. We also revealed that the stress resistance function of stringent response (SR) was partially mediated by the general stress response T6SS4 system...
March 2019: Microbiological Research
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