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Journals Clinical and Diagnostic Labora...

Clinical and Diagnostic Laboratory Immunology

https://read.qxmd.com/read/16210491/immunogenicity-of-an-intranasally-administered-modified-live-canine-parvovirus-type-2b-vaccine-in-pups-with-maternally-derived-antibodies
#41
JOURNAL ARTICLE
Vito Martella, Alessandra Cavalli, Nicola Decaro, Gabriella Elia, Costantina Desario, Marco Campolo, Giancarlo Bozzo, Elvira Tarsitano, Canio Buonavoglia
The ability of a modified live canine parvovirus type 2b vaccine to elicit active immunization in pups with maternally derived antibodies (MDA) by intranasal administration was evaluated. The vaccine induced seroconversion in 100% of pups with MDA titers of < or = 80 and in 51.6% of pups with titers between 160 and 320.
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210490/fluorescent-multivalent-opsonophagocytic-assay-for-measurement-of-functional-antibodies-to-streptococcus-pneumoniae
#42
JOURNAL ARTICLE
Kathryn T Bieging, Gowrisankar Rajam, Patricia Holder, Ross Udoff, George M Carlone, Sandra Romero-Steiner
We developed fluorescent mono- and multivalent opsonophagocytic assays (fOPA and fmOPA, respectively) specific for seven Streptococcus pneumoniae serotypes (4, 6B, 9V, 14, 18C, 19F, and 23F). Bacterial survival was quantitated with alamar blue, a fluorescent metabolic indicator. Both fOPA and fmOPA allow for determination of viability endpoints for up to seven serotypes with high levels of agreement to the reference method. The fmOPA eliminates colony counting, reduces serum volume, and produces results in 1 day...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210489/immunoglobulin-a-antibody-responses-in-dengue-patients-a-useful-marker-for-serodiagnosis-of-dengue-virus-infection
#43
JOURNAL ARTICLE
M Nawa, T Takasaki, M Ito, S Inoue, K Morita, I Kurane
We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210488/evaluation-of-liaison-treponema-screen-a-novel-recombinant-antigen-based-chemiluminescence-immunoassay-for-laboratory-diagnosis-of-syphilis
#44
COMPARATIVE STUDY
Antonella Marangoni, Vittorio Sambri, Silvia Accardo, Francesca Cavrini, Antonietta D'Antuono, Alessandra Moroni, Elisa Storni, Roberto Cevenini
The purpose of this study was to evaluate the diagnostic performance of LIAISON Treponema Screen (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with that of rapid plasma reagin (RPR) and the following currently used treponemal tests: hemagglutination test (TPHA), immunoenzymatic assay (EIA), and Western blot (WB). First, a retrospective study was performed with a panel of 2,494 blood donor sera, a panel of 131 clinical and serologically characterized syphilitic sera, and 96 samples obtained from subjects with potentially interfering diseases or conditions...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210487/lassa-fever-virus-peptides-predicted-by-computational-analysis-induce-epitope-specific-cytotoxic-t-lymphocyte-responses-in-hla-a2-1-transgenic-mice
#45
JOURNAL ARTICLE
Agnieszka Boesen, Krishnan Sundar, Richard Coico
Lassa fever is a hemorrhagic disease caused by Lassa fever virus (LV). Although the precise host defense mechanism(s) that affords protection against LV is not completely understood, cellular immunity mediated by cytotoxic T lymphocytes (CTLs) plays a pivotal role in controlling viral replication and LV infection. To date, there have been no reports mapping major histocompatibility complex (MHC) class I-binding CTL epitopes for LV. Using computer-assisted algorithms, we identified five HLA-A2.1-binding peptides of LV glycoprotein (GP) and two peptides from LV nucleoprotein (NP)...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210486/priming-of-immunological-memory-by-pneumococcal-conjugate-vaccine-in-children-unresponsive-to-23-valent-polysaccharide-pneumococcal-vaccine
#46
RANDOMIZED CONTROLLED TRIAL
Markus A Rose, Ralf Schubert, Nicola Strnad, Stefan Zielen
Pneumococcal polysaccharide vaccine (PPV) is of limited immunogenicity in infants and immunocompromised patients. Our prospective randomized controlled trial investigated whether priming with pneumococcal conjugate vaccine (PCV) induced specific immunological memory in previously nonresponders to PPV. Of a total of 33 children (2 to 18 years) with polysaccharide-specific immunodeficiency (PSI), group A (n = 16) received two doses of 7-valent PCV in a 4- to 6-week interval, and a booster dose of 23-valent PPV after one year...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210485/feline-coronavirus-serotypes-1-and-2-seroprevalence-and-association-with-disease-in-switzerland
#47
JOURNAL ARTICLE
Maya Kummrow, Marina L Meli, Michael Haessig, Enikoe Goenczi, Amy Poland, Niels C Pedersen, Regina Hofmann-Lehmann, Hans Lutz
To determine the prevalence of antibodies to feline coronavirus (FCoV) serotypes 1 and 2 in Switzerland and their association with different disease manifestations, a serological study based on immunofluorescence tests was conducted with Swiss field cats using transmissible gastroenteritis virus (TGEV), FCoV type 1 and FCoV type 2 as antigens. A total of 639 serum samples collected in the context of different studies from naturally infected cats were tested. The current study revealed that, with an apparent prevalence of 83%, FCoV serotype 1 is the most prevalent serotype in Switzerland...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210484/generation-of-feline-dendritic-cells-derived-from-peripheral-blood-monocytes-for-in-vivo-use
#48
JOURNAL ARTICLE
Giulia Freer, Donatella Matteucci, Paola Mazzetti, Leonia Bozzacco, Mauro Bendinelli
Dendritic cells (DCs) are professional antigen-presenting cells that can prime T cells and polarize the cellular immune response. Because Th1-type immune responses have been connected to success in combating viral infection, a promising therapeutic application of DCs would be their differentiation in vitro and injection back into the host to boost an immune response in infected animals. This study was aimed both at developing a protocol to cultivate feline DCs in the absence of exogenous proteins for their use in vivo and at investigating what might be the most appropriate stimulus to induce their maturation in vitro and finding correlates of maturation...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210483/diagnosis-of-invasive-pneumococcal-disease-among-children-in-kenya-with-enzyme-linked-immunosorbent-assay-for-immunoglobulin-g-antibodies-to-pneumococcal-surface-adhesin-a
#49
JOURNAL ARTICLE
J Anthony G Scott, Zena Mlacha, Joyce Nyiro, Salome Njenga, Pole Lewa, Jacktone Obiero, Hanningtone Otieno, Jacquelyn S Sampson, George M Carlone
Diagnostic techniques for invasive pneumococcal disease (IPD) in children are insensitive and underestimate both the burden of disease and the cost-effectiveness of pneumococcal conjugate vaccination (PCV). Consequently, there is little demand for the highly effective PCV outside the United States and Europe. In Kenya, diagnosis of pneumococcal pneumonia in adults was achieved with a sensitivity of 0.70 and a specificity of 0.98 using enzyme-linked immunosorbent assays (ELISAs) of paired plasma samples for immunoglobulin G (IgG) to pneumococcal surface adhesin A (PsaA)...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210482/potential-of-direct-agglutination-test-based-on-promastigote-and-amastigote-antigens-for-serodiagnosis-of-post-kala-azar-dermal-leishmaniasis
#50
JOURNAL ARTICLE
Ruchi Singh, B V Subba Raju, R K Jain, Poonam Salotra
Post-kala-azar dermal leishmaniasis (PKDL) is a dermal complication, a sequel to kala-azar. Diagnosis of PKDL presents a challenge due to the low parasite burden in the lesions. The direct agglutination test (DAT) based on promastigote and amastigote antigens of Leishmania donovani of indigenous isolates was developed to diagnose PKDL, and the results were compared with those of the rk39 strip test. The sensitivities of DAT for antileishmanial antibody detection, based on promastigote and amastigote antigens at a cutoff titer of 1:800 were 98...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210481/clonal-diversity-and-turnover-of-streptococcus-mitis-bv-1-on-shedding-and-nonshedding-oral-surfaces-of-human-infants-during-the-first-year-of-life
#51
JOURNAL ARTICLE
Jennifer L Kirchherr, George H Bowden, Dorothy A Richmond, Michael J Sheridan, Katherine A Wirth, Michael F Cole
Streptococcus mitis bv. 1 is a pioneer colonizer of the human oral cavity. Studies of its population dynamics within parents and their infants and within neonates have shown extensive diversity within and between subjects. We examined the genetic diversity and clonal turnover of S. mitis bv. 1 isolated from the cheeks, tongue, and primary incisors of four infants from birth to 1 year of age. In addition, we compared the clonotypes of S. mitis bv. 1 isolated from their mothers' saliva collected in parallel to determine whether the mother was the origin of the clones colonizing her infant...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210480/serologic-cross-reactivity-between-anaplasma-marginale-and-anaplasma-phagocytophilum
#52
JOURNAL ARTICLE
U M Dreher, J de la Fuente, R Hofmann-Lehmann, M L Meli, N Pusterla, K M Kocan, Z Woldehiwet, U Braun, G Regula, K D C Staerk, H Lutz
In the context of a serosurvey conducted on the Anaplasma marginale prevalence in Swiss cattle, we suspected that a serological cross-reactivity between A. marginale and A. phagocytophilum might exist. In the present study we demonstrate that cattle, sheep and horses experimentally infected with A. phagocytophilum not only develop antibodies to A. phagocytophilum (detected by immunofluorescent-antibody assay) but also to A. marginale (detected by a competitive enzyme-linked immunosorbent assay). Conversely, calves experimentally infected with A...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210479/cryptococcus-neoformans-reactive-and-total-immunoglobulin-profiles-of-human-immunodeficiency-virus-infected-and-uninfected-ugandans
#53
JOURNAL ARTICLE
Krishanthi Subramaniam, Neil French, Liise-Anne Pirofski
We determined total and Cryptococcus neoformans glucuronoxylomannan (GXM)-reactive antibody repertoires of human immunodeficiency virus (HIV)-infected and HIV-uninfected Ugandans in a retrospective, case-control study of participants in a randomized controlled trial of pneumococcal vaccination. The study included 192 adults: 48 who subsequently developed cryptococcal meningitis (CM); (HIV+ CM+); 2 individuals who matched them in CD4+ T-cell level, stage of HIV disease, and age but did not develop CM (HIV+ CM-); and 48 HIV-uninfected individuals...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210478/recombinant-leishmania-antigens-for-serodiagnosis-of-visceral-leishmaniasis
#54
JOURNAL ARTICLE
S Passos, L P Carvalho, G Orge, S M Jerônimo, G Bezerra, M Soto, C Alonso, E M Carvalho
Serological tests with crude or recombinant Leishmania antigens are important tools for the diagnosis of leishmania infection. However, these tests are not markers of active visceral leishmaniasis (VL), since antibodies to these markers are often observed in individuals with subclinical L. chagasi infection and they do not fall shortly after therapy. In this study, levels of immunoglobulin G (IgG) against three recombinant Leishmania antigens (rH2A, KMP11, and the "Q" protein) were evaluated in sera from individuals with subclinical L...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210477/evaluation-of-serum-antibody-responses-against-the-rotavirus-nonstructural-protein-nsp4-in-children-after-rhesus-rotavirus-tetravalent-vaccination-or-natural-infection
#55
JOURNAL ARTICLE
Esmeralda Vizzi, Eva Calviño, Rosabel González, Irene Pérez-Schael, Max Ciarlet, Gagandeep Kang, Mary K Estes, Ferdinando Liprandi, Juan E Ludert
The immune response elicited by the rotavirus nonstructural protein NSP4 and its potential role in protection against rotavirus disease are not well understood. We investigated the serological response to NSP4 and its correlation with disease protection in sera from 110 children suffering acute diarrhea, associated or not with rotavirus, and from 26 children who were recipients of the rhesus rotavirus tetravalent (RRV-TV) vaccine. We used, as antigens in an enzyme-linked immunosorbent assay (ELISA), affinity-purified recombinant NSP4 (residues 85 to 175) from strains SA11, Wa, and RRV (genotypes A, B, and C, respectively) fused to glutathione S-transferase...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210476/evaluation-of-immunoglobulin-g-subclass-antibodies-against-recombinant-fasciola-gigantica-cathepsin-l1-in-an-enzyme-linked-immunosorbent-assay-for-serodiagnosis-of-human-fasciolosis
#56
JOURNAL ARTICLE
Chaisiri Wongkham, Chairat Tantrawatpan, Pewpan M Intapan, Wanchai Maleewong, Sopit Wongkham, Kunio Nakashima
A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic infections, 32 cholangiocarcinoma patients, and 42 healthy controls, the IgG4-ELISA gave the highest diagnostic values...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210475/development-of-a-microsphere-based-serologic-multiplexed-fluorescent-immunoassay-and-a-reverse-transcriptase-pcr-assay-to-detect-murine-norovirus-1-infection-in-mice
#57
JOURNAL ARTICLE
Charlie C Hsu, Christiane E Wobus, Earl K Steffen, Lela K Riley, Robert S Livingston
Murine norovirus 1 (MNV-1) is a newly recognized pathogen of mice that causes lethal infection in mice deficient in components of the innate immune response but not in wild-type 129 mice. In this study, in vitro-propagated MNV-1 was used as antigen to develop a multiplexed fluorescent immunoassay (MFI) to detect antibodies to MNV-1 in infected mice. The MNV-1 MFI was 100% specific and 100% sensitive in detecting anti-MNV-1 antibody in sera from experimentally infected mice. Testing of a large number of mouse serum samples (n = 12,639) submitted from contemporary laboratory mouse colonies in the United States and Canada revealed that 22...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16210474/sensitivity-and-specificity-of-a-monoclonal-antibody-based-fluorescence-assay-for-detecting-enterocytozoon-bieneusi-spores-in-feces-of-simian-immunodeficiency-virus-infected-macaques
#58
JOURNAL ARTICLE
Inderpal Singh, Abhineet S Sheoran, Quanshun Zhang, Angela Carville, Saul Tzipori
Enterocytozoon bieneusi is clinically the most significant among the microsporidia causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. Microscopy with either calcofluor or modified trichrome stains is the standard diagnostic test for microsporidiosis and does not allow species identification. Detection of E. bieneusi infection based on PCR is limited to a few reference laboratories, and thus it is not the standard diagnostic assay. We have recently reported the development and characterization of a panel of monoclonal antibodies against E...
October 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16148186/patients-with-pulmonary-tuberculosis-develop-a-strong-humoral-response-against-methylated-heparin-binding-hemagglutinin
#59
JOURNAL ARTICLE
Stefania Zanetti, Alessandra Bua, Giovanni Delogu, Cinzia Pusceddu, Maristella Mura, Franca Saba, Piero Pirina, Carlo Garzelli, Cono Vertuccio, Leonardo A Sechi, Giovanni Fadda
Reactivities of human sera against selected recombinant Mycobacterium tuberculosis antigens were assessed by enzyme-linked immunosorbent assay. The results obtained indicate that patients with tuberculosis (TB) do not develop a strong humoral response against PE_PGRS and PPE proteins or against the Ag85B and heparin-binding hemagglutinin (HBHA) recombinant antigens. Conversely, purified methylated HBHA was strongly recognized by sera obtained from TB patients compared to controls.
September 2005: Clinical and Diagnostic Laboratory Immunology
https://read.qxmd.com/read/16148185/difference-in-cytokine-production-and-cell-activation-between-adenoidal-lymphocytes-and-peripheral-blood-lymphocytes-of-children-with-otitis-media
#60
JOURNAL ARTICLE
Atsushi Harimaya, Jussi Tarkkanen, Petri Mattila, Nobuhiro Fujii, Jukka Ylikoski, Tetsuo Himi
We evaluated the immunological potential of adenoidal lymphocytes from children with recurrent otitis media. Interleukin-4 release and CD69 expression were lower in adenoidal lymphocytes than in peripheral blood lymphocytes (PBL). Our results suggest that there may be a difference between the immunological potential of adenoidal lymphocytes and that of PBL in children with otitis.
September 2005: Clinical and Diagnostic Laboratory Immunology
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