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JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology

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https://read.qxmd.com/read/30630335/-is-bc-gn-applicable-for-analysis-of-strains-and-urine-sputum-specimens
#1
Tomoko Ohno, Hiroyuki Suematsu, Yuka Yamagishi, Narimi Miyazaki, Atsuko Yamada, Yuzuka Kawamoto, Daisuke Sakanashi, Isao Koita, Hiroshige Mikamo
As the reagent that can simultaneously detect bacterial nucleic acid/drug-resistant genes from the culture-positive liquid of blood cultures, Verigene® system includes the Verigene® Gram-Positive Blood Culture test (BC-GP) and the Verigene® Gram Negative Blood Culture test (BC-GN). This study used BC-GN to identify the names of bacteria from stock strains, urine samples, and sputum specimens and detect drug-resistant genes. The stock strains included 28 clinical isolates, 9 urine samples in which the target bacterial strain grew to 106CFU/ml or more in culture, and 9 sputum specimens in which the target bacterial strain grew to 105CFU/ml or more in culture...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30630334/-evaluation-of-novel-nucleic-acid-detection-kit-for-mycoplasma-pneumoniae
#2
Kazuhiro Shinto, Kyohei Kato, Taeko Narita, Hiroki Hanaiwa, Tetsuhiro Harada, Keisuke Miyako, Yumiko Funashima, Kenichi Sato, Zenzo Nagasawa, Tsukuru Umemura
For diagnosis of Mycoplasma pneumoniae infection, highly sensitive and rapid diagnosis is important. Because antibiotics are limited for the treatment of M. pneumoniae infection. In this study, we evaluated new rapid nucleic acid detection kit for M. pneumoniae . This kit does not require excessive pretreatment of specimens and molecular diagnosis of M. pneumoniae is possible within 40 min. Using 120 nasopharyngeal specimens, we compared this kit with a commercially available molecular diagnostic reagent (LAMP)...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30630333/-a-trial-of-simple-and-rapid-carbapenemase-big-five-gene-analysis-using-lamp-method
#3
Yumiko Funashima, Kazuyuki Sugahara, Yuya Hirata, Kyohei Kato, Kenichi Sato, Yasuharu Sasaki, Zenzo Nagasawa, Tsukuru Umemura
Reliable detection and typing of carbapenemase is important in the treatment of infectious diseases. In this study we newly designed LAMP primer based on the latest information, and established a detection method for Carbapenemase Big five gene. For DNA extraction from strains, alkaline boiling method and commercial kit were used. The reaction temperatures of the LAMP method was VIM: 65°C, NDM: 63°C, KPC: 65°C, OXA-48-like: 65°C, IMP: 61°C. And simultaneous LAMP method was at 63°C, for 60 min. It was possible to detect up to 103 copies/ml...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30630332/-on-the-performance-evaluation-of-versatrek-introduced-in-blood-culture
#4
Taeko Narita, Kyohei Kato, Hiroki Hanaiwa, Tetsuhiro Harada, Yukari Sano, Yumiko Funashima, Zenzo Nagasawa, Tsukuru Umemura
We evaluated performance of Versa TREK, blood culture system used in our hospital. Compared with BacT/ALERT 3D, the detection time of bacteria in the VersaTREK was shorter in most of strains. Compared with BacT/ALERT Virtuo, there was little difference in the detection time of bacteria. In addition, VersaTREK was able to detect Helicobacter cinaedi which could not be detected by other equipment, and H. cinaedi was detected in clinical specimens within 2 days. There were 147 bottles judged to be false positives at our facility, of which 7,290 were 2,0% of the total...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30630331/-on-the-identification-accuracy-of-maldi-tof-ms-influence-of-pretreatment-methods-types-of-medium-coating-skills-and-preservation-methods
#5
Hiroki Hanaiwa, Keisuke Miyako, Taeko Narita, Kyohei Kato, Kazuhiro Shinto, Yumiko Funashima, Kenichi Sato, Tetsuhiro Harada, Zenzo Nagasawa, Tsukuru Umemura
In bacterial identification by MALDI-TOF MS, there are many reports of usefulness concerning direct identification from blood culture and identification of bacteria which cannot be identified with automatic analysis equipment. On the other hand, there are very few studies that investigate how various conditions influence on identification accuracy, such as the type of medium used for bacterial isolation and pure culture, the pretreatment methods, the difference in coating technique, and preservation methods...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30630330/-bacteria-that-eat-human-lurking-in-the-ariake-sea-vibrio-vulnificus-infection
#6
Zenzo Nagasawa, Kouichi Matsumoto, Hirotaka Oishi
There are currently 76 species of bacteria in the genus Vibrio , which is a halophilic gram-negative bacillus, 12 of which are pathogenic in humans. It is usually known as a foodborn infectious bacterium related to gastrointestinal tract. Vibrio vulnificus develops muscle tissue necrosis of limb and septic shock in 1 to 3 days when infected in patients with liver injury or immune function deterioration and many die from multiple organ dysfunction. Since V. vulnificus is suitable for inhabitation and proliferation in the warm brackish water area, many infection of V...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/29562745/-detection-of-%C3%AE-lactamase-produced-by-klebsiella-pneumoniae-using-high-performance-liquid-chromatography
#7
Rika Narashima, Yuya Hasunuma, Yoshikazu Tokuoka
In order to develop a new method to detect β-lactamase, we determined degradation of β-lactam antibiotics, ampicillin sodium (ABPC) and cefotaxime sodium (CTX), by β-lactamase-producing Klebsiella pneumoniae ( K. pneumoniae ) in terms of high performance liquid chromatography (HPLC). Using HPLC with an ODS column and an eluent composed of phosphate buffer and methanol, we could detect ABPC and CTX within 10 min. After cultured with K. pneumoniae , ABPC and CTX were degraded. The degradation rate corresponding to the rate of peak area incubated with and without bacteria increased with increasing McFarland No...
March 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/29562744/-rapid-decision-of-esbl-from-blood-culture-positive-bottles-using-maldi-tof-ms-and-sysmex-uf-5000
#8
Takenori Yamashita
In recent years, the appearance of MALDI-TOF MS made it possible to identify bacterial species in a short time. However, the sensitivity test has not largely shortened the time. When ESBL is suspected by routine examination, we speculate that there are many institutions conducting the inspection method conforming to the CLSI inspection procedure. When carrying out bacteria identification, susceptibility test and ESBL confirmation test from blood culture-positive specimens, the expected examination days are required for about 3 to 4 days, which is not quick...
March 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/29562743/-legionnaires-disease-update
#9
Hiroshi Miyamoto
Legionella pneumophila , the causative agent of Legionnaires' disease, was first recognized in 1977 following an epidemic of acute pneumonia in Philadelphia, USA. Since then, a total of 59 Legionella species containing 80 serogroups have been characterized. Twenty-six of the Legionella species have been reported as pathogenic in humans. This review describes the microbiological characteristics of Legionella species, their habits in the environment, the source and route of infection, symptoms and diagnosis of Legionnaires' disease, and disease outbreaks in Japan...
March 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28817943/-maldi-tof-ms-comparison-of-blood-culture-positive-bottle-pretreatment-kit
#10
COMPARATIVE STUDY
Takenori Yamashita
Identification of bacteria of sepsis or bacteremia is a useful result for treatment policy. In recent years, bacterial identification has become possible from blood culture bottles by MALDI‒TOF, but it is not as accurate as bacterial identification from agar colonies. Blood culture pretreatment kit (MALDI Sepcityper Kit) is currently on sale from Bruker. However, the current situation has not reached good accuracy. This time, a new blood culture pretreatment kit appeared, so I studied. Up to now, the blood culture pretreatment kit was only MALDI Sepcityper Kit using enzyme digestion method...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28817942/-the-current-status-of-diagnostic-tools-for-leptospirosis
#11
REVIEW
Mitsumasa Saito, Yasuhiko Nikaido, Masahiro Matsumoto, Midori Ogawa, Sharon Y A M Villanueva
Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. The severity of leptospirosis vary from mild, flu-like disease to a more severe form, Weil's disease causing jaundice, hemorrhage, renal failure, and even death. Every year, 300,000‒500,000 cases of severe leptospirosis are reported around the world, with the case fatality rate being 10‒30%. The usual diagnostic tools for leptospirosis are 1) direct observation of leptospires in blood and urine under dark-field microscope, 2) isolation of leptospires from blood, cerebrospinal fluid (CSF), or urine samples by culture, 3) microscopic agglutination test (MAT) to detect anti- Leptospira antibodies in serum, and 4) PCR to detect Leptospira DNA...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28817941/-a-case-of-severe-legionella-longbeachae-pneumonia-and-usefulness-of-lamp-assay
#12
Kumiko Matsushita, Kohei Hijikuro, Shohei Arita, Yu Kaneko, Masahiro Isozaki
Urinary antigen test is frequently used as a routine laboratory test for early diagnosis of Legionella infection , which is especially suitable for ordinary Legionella pneumophila serogroup 1, but not for other types of Legionella . We report a case of severe pneumonia caused by Legionella longbeachae , where a method of loop-mediated isothermal amplification (LAMP) assay contributed an important role for the early detection. This case involved an 83-year-old man who developed fever, dyspnea, and productive cough...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28817940/-confirming-the-utility-of-raisus-antifungal-susceptibility-testing-by-new-software
#13
Tomoko Ono, Hiroyuki Suematsu, Haruki Sawamura, Yuka Yamagishi, Hiroshige Mikamo
Clinical and Laboratory Standards Institute (CLSI) methods for susceptibility tests of yeast are used in Japan. On the other hand, the methods have some disadvantage; 1) reading at 24 and 48 h, 2) using unclear scale, approximately 50% inhibition, to determine MICs, 3) calculating trailing growth and paradoxical effects. These makes it difficult to test the susuceptibility for yeasts. Old software of RAISUS, Ver. 6.0 series, resolved problem 1) and 2) but did not resolve problem 3). Recently, new software of RAISUS, Ver...
August 15, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28274129/-utility-of-the-rapid-staining-with-the-use-of-microwave-for-detection-of-genus-mycobacterium
#14
Yumiko Funashima, Kyohei Kato, Taeko Narita, Hiroki Hanaiwa, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Kazuyuki Sugahara, Hiroshi Miyamoto
Recently, many laboratories use fluorescence microscopy for rapid screening of clinical specimens for detection of Genus Mycobacterium. The success of the stain depends on the staining temperature at which the fluorescent dye could uniformly penetrate the cell wall through waxy lipid barrier of the mycobacterial organism. Therefore, this process requires a precise heating control. In this study, to control the temperature during fluorescent auramine- rhodamine staining, we explored the potential use of microwave...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28274128/-evaluation-of-a-new-immunochromatographic-kit-for-enhanced-detection-of-influenza-b-virus
#15
Yasushi Ashikawa, Yoshio Takasaki, Shizuo Shindo, Yuji Yamashita, Keigo Shibao, Takashi Yokoyama, Takato Yokoyama, Minako Iwaya, Yumi Kiyomatsu, Hiroshi Miyamoto
We have evaluated a new immunochromatographic kit, "KBM LineCheck Flu AB", which had been developed for enhanced detection of influenza B viruses. Five strains of influenza A and B viruses were tested for reactivity and detection limits of the kit. Compared with the detection limits of commercially available kit of QuickNavi-Flu, "KBM LineCheck Flu AB" showed a nearly equal reactivity to influenza A viruses, but quadruple reactivity to 2 influenza B viruses. Also, "KBM LineCheck Flu AB" exhibited high specificity when tested in 130 influenza-negative culture specimens derived from 24 adult volunteers...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28274127/-development-and-evaluation-of-a-new-selective-culture-medium-kbm-anaero-rs-gnr-for-detection-of-anaerobic-gram-negative-rods
#16
Taeko Narita, Kyohei Kato, Hiroki Hanaiwa, Tetsuhiro Harada, Yumiko Funashima, Makoto Akiwa, Jun-Ichiro Sekiguchi, Zenzo Nagasawa, Tsukuru Umemura
The laboratory culture methods for isolating drug-resistant pathogens has been the gold standard in medical microbiology, and play pivotal roles in the overall management of infectious diseases. Recently, several reports have emphasized the development of antibiotics-resistance among anaerobic gram-negative rods, especially Genus Bacteroides and Prevotella. Therefore, a selective culture method to detect these pathogens is needed. We developed here the new selective culture medium, termed "KBM Anaero RS-GNR," for detecting anaerobic Gram-negative rods...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/28274126/-selection-of-laboratory-procedures-to-detect-toxigenic-by-the-2-step-method
#17
Yoko Tanino, Mai Kodama, Hiroomi Daicho, Yoshito Miyauchi, Towa Yasumoto, Yukiji Yamada, Noriko Kyotani, Satoko Kurahashi, Masaji Ushiyama, Takeshi Kimura, Toshiaki Komori, Yumiko Fujitomo, Masaki Nakanishi, Naohisa Fujita
The 2-step method is an algorithm to detect toxigenic Clostridium difficile. We herein compared the sensitivities and specificities of an enzyme immunoassay (toxin A/B-EIA), toxigenic culture (TC-EIA), Loop-Mediated Isothermal Amplification assay (LAMP), and Xpert C. difficile (Xpert) with the detection of the toxin B gene by a polymerase chain reaction (PCR). The results obtained showed that the sensitivities and specificities of toxin A/B-EIA, Xpert, TC-EIA, and LAMP were 30 and 100%, 87.2 and 100%, 97.5 and 89...
March 22, 2017: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/26437475/-abstracts-of-the-27th-annual-meeting-of-the-japanese-society-of-clinical-toxicology-july-4-2015-kanazawa-japan
#18
(no author information available yet)
No abstract text is available yet for this article.
July 2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/26790486/retraction-aeromonas-species-infection-with-severe-clinical-manifestation-in-okinawa-japan-association-with-gas-gangrene
#19
(no author information available yet)
Following a strict, extensive, and judicious review, it has become clear that the paper by Nakasone et al deviates from the ethical standard of authorship. Therefore, the Editor-in-Chief, Hiroyuki Nishiyama, has agreed to fully retract this paper from publication and from the published record.
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/26635004/-rapid-identification-method-positive-blood-culture-bottles-with-maldi-biotyper
#20
Mina Fukuoka, Hitoshi Miyamoto, Shinobu Murakami, Yuri Tanaka, Takuya Kondo, Tatsuya Nishimiya, Haruhiko Osawa
Identification method positive blood culture bottles with MALDI Biotyper is the most important test on precisely and rapidly, for detamination the bacterial name in sepsis and bacteremia is very significant for decision a cure. This time, we devised a new method "blend" to identify the mixture hypostasis that were come into being by centrifuging blood culture broths and 70% formic acid with MALDI Biotyper (Bruker). This time, we identified 65 samples rapidly with MALDI biotyper by "on plate" and "blend," and verified their effectivity...
2015: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
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