Jana Štundlová, Monika Hospodářská, Karolína Lukšíková, Anna Voleníková, Tomáš Pavlica, Marie Altmanová, Annekatrin Richter, Martin Reichard, Martina Dalíková, Šárka Pelikánová, Anatolie Marta, Sergey A Simanovsky, Matyáš Hiřman, Marek Jankásek, Tomáš Dvořák, Joerg Bohlen, Petr Ráb, Christoph Englert, Petr Nguyen, Alexandr Sember
Homomorphic sex chromosomes and their turnover are common in teleosts. We investigated the evolution of nascent sex chromosomes in several populations of two sister species of African annual killifishes, Nothobranchius furzeri and N. kadleci, focusing on their under-studied repetitive landscape. We combined bioinformatic analyses of the repeatome with molecular cytogenetic techniques, including comparative genomic hybridization, fluorescence in situ hybridization with satellite sequences, ribosomal RNA genes (rDNA) and bacterial artificial chromosomes (BACs), and immunostaining of SYCP3 and MLH1 proteins to mark lateral elements of synaptonemal complexes and recombination sites, respectively...
October 8, 2022: Chromosome Research