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Wound Repair and Regeneration

Qinhui Liu, Shiyun Pu, Lei Chen, Jing Shen, Shihai Cheng, Jiangying Kuang, Hong Li, Tong Wu, Rui Li, Wei Jiang, Min Zou, Zhiyong Zhang, Yanping Li, Jian Li, Jinhan He
Sirtuin 6 (Sirt6) is an NAD+-dependent deacetylase that regulates central metabolic functions such as glucose homeostasis, fat metabolism and cell apoptosis. However, the tissue-specific function of Sirt6 in liver regeneration remains unknown. Here, we show that liver-specific Sirt6 knockout (Sirt6 LKO) impaired liver reconstitution after 2/3 partial hepatectomy, which was attributed to an alteration of cell cycle progression. Sirt6 LKO delayed hepatocyte transition into S phase during liver regeneration, as shown by the analysis of cell cycle-related proteins and the immunostaining of Ki-67 and 5-bromo-2-deoxyuridine (BrdU)...
January 31, 2019: Wound Repair and Regeneration
Ju Tian, Liu-Hang-Hang Cheng, Xiao Cui, Xiao-Xuan Lei, Jian-Bing Tang, Biao Cheng
In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1069-1436×109 /L...
January 28, 2019: Wound Repair and Regeneration
Hassan Afzali, Amir Jafari Kashi, Mansooreh Momen-Heravi, Reza Razzaghi, Elaheh Amirani, Fereshteh Bahmani, Hamid Reza Gilasi, Zatollah Asemi
This study was carried out to determine the effects of magnesium and vitamin E co-supplementation on wound healing and metabolic status in patients with diabetic foot ulcer (DFU). The current randomized, double-blind, placebo-controlled trial was conducted among 57 patients with grade 3 DFU. Participants were randomly divided into two groups to take either 250 mg magnesium oxide plus 400 IU vitamin E (n=29) or placebo per day (n=28) for 12 weeks. Compared with the placebo, taking magnesium plus vitamin E supplements reduced ulcer length [β (difference in the mean of outcomes measures between treatment groups) -0...
January 28, 2019: Wound Repair and Regeneration
J A Jaller, E S Darwin, J I Silverberg, H A Lev-Tov
Venous Leg Ulcers (VLU) comprise about 70% of lower leg wounds and cost more than 14 billion USD per year. Patients with VLU are often admitted to the hospital; however, epidemiological study of this important patient subset is challenged by lack of validated database search protocol. Five International Classification of Diseases (ICD) 9 codes were identified for their relationship with VLUs (454.0, 454.2, 459.11, 707.9, 707.10). The ICD9 code was considered to successfully predict a VLU if the wound was located in the gaiter area and had either relevant clinical findings, a positive venous study, and/or a diagnosis of VLU written in the physician note...
January 28, 2019: Wound Repair and Regeneration
Zjir M Rashaan, Pieta Krijnen, Kelly A A Kwa, Cornelis H van der Vlies, Inger B Schipper, Roelf S Breederveld
Although partial thickness burns are the most frequently reported burn injuries, there is no consensus on the optimal treatment. The objective of this study was to compare the clinical effectiveness and scar quality of Flaminal® Forte to silver sulfadiazine (Flamazine®) in the treatment of partial thickness burns. In this two-arm open label multi-center randomized controlled trial, adult patients with acute partial thickness burns and an affected total body surface area of less than 30% were randomized between Flaminal® Forte and Flamazine® and followed for 12 months...
January 24, 2019: Wound Repair and Regeneration
Jong-Heng Yapp, Raja Kamil, M Rozi, Norhafizah Mohtarrudin, M Y Loqman, A R Ezamin, Siti Anom Ahmad, Zuki Abu Bakar
Frequent repositioning is important to prevent pressure ulcer (PU) development, by relieving pressure and recovering damages on skin areas induced by repetitive loading. Although repositioning is the gold standard to prevent PU, there is currently no strategy for determining tissue condition under preventive approaches. In this study, the peak reactive hyperemia (RH) trends and ultrasonographic (US) features are compared with the tissue condition under histopathological examination to determine the potential use of these features in determining the tissue condition non-invasively...
January 22, 2019: Wound Repair and Regeneration
Allyson R Alfonso, Rami S Kantar, Elie P Ramly, David A Daar, William J Rifkin, Jamie P Levine, Daniel J Ceradini
The effect of diabetes on postoperative outcomes following surgical management of pressure ulcers is poorly defined despite evidence showing that patients with diabetes are at increased risk for developing pressure ulcers, as well as postoperative wound complications including delayed healing and infection. This study aimed to examine the impact of diabetes on postoperative outcomes following surgical management of pressure ulcers using the American College of Surgeons National Surgical Quality Improvement Program (ACS-NSQIP) database...
January 21, 2019: Wound Repair and Regeneration
Diana Lelli, Claudio Pedone, Elena Frezzotti, Giorgio Pennazza, Marco Santonico, Simone Grasso, Raffaele Antonelli Incalzi
The timely recognition of leg ulcers (LU) aetiology and infection is pivotal to optimize management and accelerate healing. The objective of this proof-of-concept study was to test the diagnostic performance of voltammetric analysis (VA) on ulcer exudate to identify LU aetiology, infection and predict clinical course. We enrolled 25 patients aged ≥60 years, affected by 42 venous/arterial LU. Clinical examination (Leg Ulcer Measurement Tool score, LUMT), swab culture, and VA were performed at baseline and 30 days...
January 21, 2019: Wound Repair and Regeneration
Kevin M Klifto, Amy Quan, A Lee Dellon
Mary Edwards Walker (1832-1919) was the first female surgeon in the United States. Her upbringing and unique medical training led her to practice medicine in a way that was revolutionary for the time. During the Civil War, her approach to wound care rivaled the current standard of care. During an era that predated antiseptic surgical technique, she prioritized cleanliness and hygiene. She opposed amputation for its surgical risks and decreased postoperative quality of life. She believed that many wounds, when appropriately attended to, would heal without amputation...
January 21, 2019: Wound Repair and Regeneration
Aos S Karim, Amy Yan, Edgar Ocotl, Daniel D Bennett, Ziyue Wang, Christina Kendziorski, Angela L F Gibson
The regenerative capacity of burn wounds, and the need for surgical intervention, depends on wound depth. Clinical visual assessment is considered the gold standard for burn depth assessment but it remains a subjective and inaccurate method for tissue evaluation. The purpose of this study was to compare visual assessment with microscopic and molecular techniques for human burn depth determination, and illustrate differences in the evaluation of tissue for potential regenerative capacity. Using intraoperative visual assessment, patients were identified as having deep partial thickness or full thickness burn wounds...
December 26, 2018: Wound Repair and Regeneration
Eric D Miller, Feifei Song, Jason D Smith, Ahmet S Ayan, Xiaokui Mo, Michael Weldon, Lanchun Lu, Phil G Campbell, Aashish D Bhatt, Arnab Chakravarti, Naduparambil K Jacob
Cutaneous wounds caused by an exposure to high doses of ionizing radiation remain a therapeutic challenge. While new experimental strategies for treatment are being developed, there are currently no off-the-shelf therapies for the treatment of cutaneous radiation injury that have been proven to promote repair of the damaged tissues. Plasma-based biomaterials are biologically active biomaterials made from platelet enriched plasma, which can be made into both solid and semi-solid forms, are inexpensive, and are available as off-the-shelf, nonrefrigerated products...
December 21, 2018: Wound Repair and Regeneration
Tasmania Del Pino-Sedeño, María M Trujillo-Martín, Isabel Andia, Javier Aragón-Sánchez, Estefanía Herrera-Ramos, Francisco J Iruzubieta Barragán, Pedro Serrano-Aguilar
Foot ulcer is a major complication of diabetes mellitus and often precedes leg amputation. Among the different methods to achieve ulcer healing, the use of platelet-rich plasma, which is rich in multiple growth factors and cytokines and may have similarities to the natural wound healing process, is gaining in popularity. A systematic review with meta-analyses was performed to evaluate the safety and clinical effectiveness of platelet-rich plasma for the treatment of diabetic foot ulcers compared to standard treatment or any other alternative therapy...
December 21, 2018: Wound Repair and Regeneration
Nur Azida Mohd Nasir, Ralf Paus, David M Ansell
Ex vivo wounded human skin organ culture is an invaluable tool for translationally relevant preclinical wound healing research. However, studies incorporating this system are still underutilized within the field because of the low throughput of histological analysis required for downstream assessment. In this study, we use intravital fluorescent dye to lineage trace epidermal cells, demonstrating that wound re-epithelialization of human ex vivo wounds occurs consistent with an extending shield mechanism of collective migration...
December 21, 2018: Wound Repair and Regeneration
Valerio Cozza, Marco M Pascale, Gilda Pepe, Rocco Panzera, Sabina Magalini, Daniele Gui
Surgical site infections represent one of the most common surgical complications. Negative pressure wound therapy is considered an effective wound management system, based on the principle that a negative pressure inside the wound can suction fluids and approximate wound edges. With the negative pressure wound therapy systems commercially available it is assumed that the pressure inside the wound is stable at the set values. We conducted a prospective experimental study to investigate this. The negative pressure level achieved inside the dressing was investigated at a standard distance from the pad of suction and at specific times in patients with surgical site infections...
December 12, 2018: Wound Repair and Regeneration
Julian Camill Harnoss, Qasim Mahmoud Abu Elrub, Jin-On Jung, Torsten Koburger, Ojan Assadian, Joachim Dissemond, Romy Baguhl, Roald Papke, Axel Kramer
Antiseptics are being used for prevention of infections in acute wounds and for treatment of infections in acute and chronic wounds. However, some antiseptics' high tissue toxicity might delay the healing process. The aim of this study was to investigate the tissue toxicity of preferentially used wound antiseptics and the influence of antiphlogistic additives via the hen's egg test on the chorioallantoic membrane (HET-CAM). The HET-CAM is a semi-in-vivo method testing the tissue tolerability of wound antiseptics by evaluating the blood vessel reaction of the chorioallantoic membrane in terms of hemorrhage, vessel lysis, and coagulation...
December 5, 2018: Wound Repair and Regeneration
Huaxiu Luo, Chang Cao, Jian Zhong, Junjie Chen, Ying Cen
Dressing change and physical therapy are extremely painful procedures for burn patients. Adjunctive virtual reality therapy reportedly reduces pain when added to analgesics, but a summary analysis of the data has yet to be performed. We conducted this systematic review and meta-analysis of randomized controlled trials to verify the pain-reducing efficacy of virtual reality among burn patients undergoing dressing change or physical therapy. We searched MEDLINE (via PubMed), EMBASE (via OVID), and the Cochrane Central Register of Controlled Trials (via OVID) for relevant trials based on predetermined eligibility criteria from database establishment to February 2018...
November 27, 2018: Wound Repair and Regeneration
Changsik Pak, Ji In Jeon, Hyeonwoo Kim, Jungyoon Kim, Suyeon Park, Ki-Hwan Ahn, Yeon-Joo Son, Sooyoung Yoo, Rong-Min Baek, Jae Hoon Jeong, Chan Yeong Heo
We investigated the accuracy of pressure injury evaluation using tele-devices and examined the concordance between automatically generated recommendations and primary manual recommendations. Caregivers took photos and videos of pressure injuries using smartphones with built-in cameras and uploaded the media to the application. The wound team evaluated the wound using a specially modified version of the Pressure Sore Status Tool. This was compared with the Pressure Sore Status Tool score assessed during the actual examination of the patient...
December 2018: Wound Repair and Regeneration
Rong-Fu Chen, Chih-Hau Chang, Chun-Ting Wang, Ming-Yu Yang, Ching-Jen Wang, Yur-Ren Kuo
Extracorporeal shockwave therapy (ESWT) has a significant positive effect to accelerate chronic wound healing. This study investigated whether the vascular endothelial growth factor (VEGF)-related pathway has involved in ESWT enhancement of diabetic wound healing. A dorsal skin defect (area, 6 × 5 cm) in a streptozotocin-induced diabetes rodent model was used. Thirty-two male Wistar rats were divided into four groups. Group I consisted of nondiabetic control; group II, diabetic control without treatment; group III, diabetic rats received ESWT; and group IV, rats received Avastin (a VEGF monoclonal antibody) on day 0 (post-wounding immediately) to day 7 and ESWT on day 3 and day 7...
November 5, 2018: Wound Repair and Regeneration
Kristo Nuutila, Lu Yang, Michael Broomhead, Karl Proppe, Elof Eriksson
Negative pressure wound therapy (NPWT) promotes healing in acute or chronic wounds. Conventional NPWT devices consist of a filler (such as foam or gauze) that covers the wound and of a permeable membrane and tubing that connects the space under the membrane to a suction pump. The permeable membrane increases airflow and thus increases the required pump capacity that can cause patient discomfort or even ischemia in wounds with compromised vascularity. In addition, foam or gauze may fragment and become colonized with bacteria over time...
October 31, 2018: Wound Repair and Regeneration
Ricardo Ginestal, Bárbara Perez-Köhler, Paloma Pérez-López, Marta Rodríguez, Gemma Pascual, David Cebrián, Juan M Bellón, Francisca García-Moreno
In this study, rat models of wound closure by first and second intention were developed to evaluate the influence that two immunosuppressants for treating multiple sclerosis (fingolimod, azathioprine) have on wound healing. Sixty-three Sprague-Dawley rats were daily treated with fingolimod (0.6 mg/kg), azathioprine (2.5 mg/kg) or placebo (saline). Following 6 weeks of treatment, a linear incision (1.5 cm) or a circular excisional defect (diameter 1.5 cm) was made on the dorsal skin. The treatments were uninterrupted and after 7 days (incisional) or 21 days (incisional, excisional), animals were euthanized (n = 7 per group and time-point)...
October 28, 2018: Wound Repair and Regeneration
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