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Cell Biology International

Yinan Zhou, Xin Li, Xueying Yuan, Lirong Hao
Peritoneal dialysis (PD) is often used to treat patients with end stage renal disease, and its long-term complications include excessive inflammation and oxidative stress. Allograft inflammatory factor 1 (AIF-1), as a cytoplasmic protein, is originally identified from infiltrating macrophages, and it was associated with inflammation in the cells other than macrophages, such as endothelial cells and vascular smooth muscle cells. To clarify the molecular mechanisms of AIF-1-modulated pathological changes in the peritoneum during PD, we first detected the AIF-1 expression in peritoneal tissues from PD mice...
February 13, 2019: Cell Biology International
Naguib Salleh, Abu Sadat Md Sayem, Nelli Giribabu, Si Lay Khaing
Hypothyroidism has been linked to infertility, but the mechanisms underlying infertility-related hypothyroidism have yet to be fully elucidated. Therefore, in this study, effects of hypothyroidism on expression of the proteins related to thyroid hormone function in the uterus, which were thought to play a role implantation, including thyroid hormone receptor (TR), thyroid stimulating hormone receptor (TSHR), retinoic acid receptor (RAR) and extracellular kinase (ERK) were identified. Pregnant female rats were rendered hypothyroid by giving methimazole (MMI), orally...
February 13, 2019: Cell Biology International
Panita Panchamanon, Prasit Pavasant, Chidchanok Leethanakul
Mechanical stimuli have been shown to play an important role in directing stem cell fate and maintenance of tissue homeostasis. One of the functions of the mechanoresponsive tissue periodontal ligament (PDL) is to withstand the functional forces within the oral cavity. Periodontal ligament stem cells (PDLSCs) derived from periodontal tissue have been demonstrated to be able to respond directly to mechanical forces. However, the mechanisms of action of mechanical force on PDLSCs are not totally understood. The aim of this study was to investigate the mechanisms by which compressive force affects PDLSCs, especially their stemness properties...
February 13, 2019: Cell Biology International
Zahra Shabani Azandaryani, Nahid Davoodian, Afshin Samiei, Sahere Rouzbehan
There are numerous studies which provide support for the use of human adipose tissue-derived stem cells (hASCs) to generate hepatocyte-like cells. However, the produced cells exhibit only a certain level of differentiation, mainly due to inefficient induction conditions. Therefore, based on the important role of insulin-like growth factor (IGF-I) in hepatic function and development, in the current study we evaluated the differentiation efficacy of the mentioned factor to induce hASCs into functional hepatocyte-like cells...
February 13, 2019: Cell Biology International
Mariana Soares Pena Ribeiro, Danielle Pereira Silva, Isadora Akemi Uehara, Erivan Schnaider Ramos-Junior, Paulo Vinicius Gil Alabarse, Sandra Yasuyo Fukada Alves, Felipe Cordero da Luz, Leda Quercia Vieira, Ana Paula Lima Oliveira, Marcelo José Barbosa Silva
Reactive oxygen species (ROS) are produced by NADPH oxidase (NOX), an enzyme that reduces oxygen by using NADPH as a substrate. Apocynin (APO) is a catechol that is used as a NOX inhibitor, and N-acetyl-cysteine (NAC) can reduce intracellular ROS levels. In this work, the effect of APO and NAC on osteoclast formation were evaluated. APO and NAC significantly decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive cells and the osteoclast area. We analyzed bone-marrow derived monocyte-macrophages (BMMs) that differentiated into osteoclasts after RANKL stimulation...
February 13, 2019: Cell Biology International
Mariya M Mikhailova, Andrey A Panteleyev, Mikhail A Paltsev, Andrey A Panteleyev
It is a well-known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g., co-culture of neural and endothelial cells) to in vivo imaging of central neural system recovery in laboratory animals after artificially induced trauma. Nevertheless, most of these models have serious limitations. Here, we describe an ex vivo model, representing an organotypic co-culture of aortic fragments (AF) with longitudinal slices of mouse neonatal spinal cord (SC) or dorsal root ganglia (DRG)...
February 13, 2019: Cell Biology International
Scheilla Teixeira Strumillo, Marli Ferreira Curcio, Fábio Ferreira de Carvalho, MariaCecília Araripe Sucupira, Ricardo Sobhie Diaz, Hugo Pequeno Monteiro, Luiz Mário Ramos Janini
Although interleukin-24 (IL-24) has been extensively explored in the immunopathologies of autoimmune diseases, neoplasms, and infections, its role in HIV-1 infection has not been thoroughly elucidated to date. Therefore, the objective of this study was to evaluate the gene and protein expressions of IL-24 at the initial moments of HIV infection in PBMCs. Due to the pro-apoptotic role of IL-24, we evaluated the protein expression of caspase-3, as well as Annexin V/ Propidium Iodide flow cytometry and phosphorylation of ERK, which may induce an apoptotic signal block when phosphorylated...
February 13, 2019: Cell Biology International
Monika Buhl, Tomasz Kloskowski, Arkadiusz Jundzill, Maciej Gagat, Daria Balcerczyk, Jan Adamowicz, Alina Grzanka, Maciej Nowacki, Gerard Drewa, Dorota Olszewska, Tomasz Drewa, Marta Pokrywczynska
Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative and self-renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone-, meroclone-and paraclone-like colonies were identified based on their morphology...
February 6, 2019: Cell Biology International
Yiyu Sun, Bolun Lu, Jingcheng Deng, Zhaohua Jiang, Weigang Cao, Tingting Dai, Shengli Li
Our study was designed to investigate the effects of IL-7 during the differentiation process of adipose-derived stem cells (ADSCs) toward lymphatic endothelial cells (LECs). IL-7 was added to the traditional induced medium, which was called the IL-7 (+) group, while the group that used traditional induced medium was called the IL-7 (-) group. After 7 days of induction of ADSCs, a comprehensive analysis was conducted between these two groups. We examined the changes in Prox1, LYVE-1, Podoplanin and VEGFR-3 on the RNA and protein level and found that the expression of LEC markers in the IL-7 (+) group was higher than in the IL-7 (-) group...
January 28, 2019: Cell Biology International
Claudio L Q Bastos, Marcelo Estrella Josende, Shana Pires Ferreira, Mariana Torquato Quezado de Magalhães, Adriano Monteiro Castro de Pimenta, Juliane Ventura Lima, Robert Tew Boyle
In this study we provide new evidence that the columnar vesicles of the sea anemone Bunodosoma cangicum are toxic in vivo and contain at least two active polypeptides, a neurotoxic and an apoptosis inducing polypeptide. Here we show that it is also an effective inducer of apoptosis in vivo in the nematode Caenorhabditis elegans. In addition, the anemone peptides rapidly paralyze C. elegans, and set in motion a sequence of events that result in the complete dissolution of the internal organs in adult animals within 60 minutes...
January 23, 2019: Cell Biology International
Azam Bolhassani
The successful intracellular delivery of biologically active proteins and peptides plays an important role for therapeutic applications. Indeed, protein/ peptide delivery could overcome some problems of gene therapy, e.g., controlling the expression levels and the integration of transgene into the host cell genome. Thus, protein/ peptide drug delivery showed a promising and safe approach for treatment of cancer and infectious diseases. Due to the unique physical and chemical properties of proteins, their production (e...
January 23, 2019: Cell Biology International
Long Yan, Zhanchuan Liu, Haoyuan Yin, Zhenjie Guo, Qi Luo
Atherosclerosis (AS) is one of the most common chronic inflammatory diseases and a major cause of morbidity and mortality. However, the underlying molecular mechanisms of the progression of AS are still largely unknown. Increasing evidence has demonstrated that long noncoding RNAs (lncRNAs) play important roles in a variety of biological processes and the physiological and pathological progression of human diseases. In this study, we aimed to explore the role and underlying molecular mechanism of lncRNA MEG3 in Raw264...
January 23, 2019: Cell Biology International
Jun Ma, Li Chen, Xiao-Xiao He, Ya-Jun Wang, Hua-Li Yu, Zi-Xuan He, Lu-Qing Zhang, Yao-Wu Zheng, Xiao-Juan Zhu
Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in acetyl-coenzyme A (acetyl-CoA) synthesis. We then used the most characterized member, disconnected interacting protein 2 homolog A (DIP2A), for further study. DIP2A is a cytoplasmic protein that is preferentially localized to mitochondria, and its acetyl-CoA synthetase activity has been demonstrated in vitro...
January 23, 2019: Cell Biology International
Guoqiang Huang, Xiaopeng Liu, Xiaoying Zhao, Jing Zhao, Jinxia Hao, Juan Ren, Ying Chen
MiR-9 has been reported to play a pivotal role in multiple human cancers through acting as an oncogene or tumor suppressor. In this study, we explored the possible role and molecular mechanism of miR-9 in multiple myeloma (MM). MiR-9 expression was examined by qRT-PCR assay. Transfection with miR-9-mimics, miR-9-inhibitor, pcDNA-TRIM56 or si-TRIM56 into cells was used to change the expression levels of miR-9 and TRIM56. Western blot analysis was used to detect the expression of TRIM56, p65, p-p65, IκBα and p-IκBα...
January 14, 2019: Cell Biology International
Monika Gasiūnienė, Aistė Zentelytė, Gražina Treigytė, Sandra Baronaitė, Jūratė Savickienė, Algirdas Utkus, Rūta Navakauskienė
Amniotic fluid-derived mesenchymal stem cells (AF-MSCs) are autologous to the fetus and represent a potential alternative source for the regenerative medicine and treatment of perinatal disorders. To date, AF-MSCs differentiation capacity to non-mesodermal lineages and epigenetic regulation are still poorly characterized. The present study investigated the differentiation potential of AF-MSCs towards neural-like cells in comparison to the mesodermal myogenic lineage and assessed epigenetic factors involved in tissue-specific differentiation...
January 11, 2019: Cell Biology International
Chen Hu, Jianhui Sun, Juan Du, Dalin Wen, Hongxiang Lu, Huacai Zhang, Yuqi Xue, Anqiang Zhang, Ce Yang, Ling Zeng, Jianxin Jiang
Regeneration of pulmonary epithelial cells plays an important role in the recovery of acute lung injury (ALI) which is defined by pulmonary epithelial cell death. However, the mechanism of the regenerative capacity of alveolar epithelial cells is unknown. Using a lung injury mouse model induced by hemorrhagic shock and LPS, a protein mass spectrometry-based high-throughput screening and linage tracing technology to mark alveolar epithelial type 2 cells (AEC2s), we analyzed the mechanism of alveolar epithelial cells proliferation...
January 11, 2019: Cell Biology International
Jae-Nyoung Heo, Dong-Yeon Kim, Su-Geun Lim, Kiboo Lee, Kyungho Suk, Won-Ha Lee
The functional and physical interaction between mitochondria and the endoplasmic reticulum (ER) has been the subject of intense study. To test the effect of this interaction on macrophage inflammatory activation, the human macrophage-like monocytic leukemia cell line THP-1 was treated with oligomycin, rotenone, or sodium azide, which induce mitochondrial dysfunction (MD) by blocking the electron transport chain (ETC). MD induced by these agents triggered activation of various sensors and markers of ER stress...
January 11, 2019: Cell Biology International
Miao Hu, Xiangping Li, Junli Zhang, Zengyan Yuan, Yuping Fu, Xiaojuan Ma, Nannan Ren
Prolonged inflammation and deregulated cytokine production are associated with diversified inflammatory diseases. Genistein (GEN), the active and predominant isoflavonoid in dietary soybean, possesses anti-inflammatory activity. Our study aimed to assess the anti-inflammatory effects of GEN-27, a derivative of GEN, as well as explore the potential molecular mechanisms using lipopolysaccharide (LPS)-induced RAW264.7 cells. In our study, we demonstrated that GEN-27 administration (1, 5, or 10 µM) dose-dependently inhibited nitrite and nitric oxide (NO) levels in LPS-stimulated RAW264...
January 11, 2019: Cell Biology International
Xiaoyong Lu, Mo Chen, Jingfang Shen, Yujia Xu, Huaxiang Wu
Long-standing untreated hyperuricemia could lead to gout. Several recent studies have demonstrated a significant decrease of serum urate during acute gout attack, which is an aseptic inflammation process focusing on IL-1β. However, how IL-1β, by itself, alters the expression and the functional activity of urate transporters in renal tubular epithelial cells is still unclear. Herein, we revealed that IL-1β could attenuate the mRNA and protein levels of ABCG2, a major urate efflux pump, in HK-2 cells by real-time PCR and western-blot assays...
January 11, 2019: Cell Biology International
Yaoyao Fang, Zhiyuan Xue, Lianggong Zhao, Xiuyan Yang, Yafei Yang, Xianglin Zhou, Shilan Feng, Keming Chen
Calycosin has been reported to have a strong osteogenic activity and a positive correlation with anti-osteoporosis effects. However, its precise mechanism of action remains unclear. Since insulin-like growth factor 1 receptor (IGF1R) signaling and phosphatidylinositol 3-kinase/ Akt (PI3K/Akt) signaling have been shown to play a pivotal role in regulating osteogenesis, we hypothesized that the osteogenic activity of calycosin is mediated by these signaling pathways. Rat calvarial osteoblasts (ROBs) were cultured in osteogenic medium containing calycosin with or without GSK1904529A (GSK) or LY294002 (LY) (inhibitors of IGF1R and PI3K, respectively)...
January 11, 2019: Cell Biology International
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