journal
https://read.qxmd.com/read/37101956/a-mammary-fibroadenoma-with-terminal-end-buds-like-structures-in-a-7-week-old-male-sd-rat
#21
Yumiko Kamiya, Tsubasa Saito, Moeko Aoki, Mizuho Takagi, Kochi Kakimoto, Yuko Yamaguchi
We report a case of mammary fibroadenoma in a 7-week-old male SD rat. This case showed rapid growth within one week from the time when the nodule was detected. Histologically, the nodule was a well-circumscribed subcutaneous mass. The tumor consisted of an epithelial component with island-like proliferation (cribriform to tubular patterns) and an abundant mesenchymal component. Alpha-SMA-positive cells were arranged at the periphery of the epithelial component and showed cribriform and tubular patterns. Discontinuous basement membranes and high cell proliferative activities were observed in the cribriform area...
April 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36683728/safety-and-efficacy-of-a-novel-robotic-fractional-micro-coring-device-in-a-swine-model
#22
JOURNAL ARTICLE
Yuval Ramot, Udi Vazana, Orna Cacical, Abraham Nyska
Laser resurfacing may be accompanied by unwanted side effects. The micro coring technology, designed to remove small skin columns, was developed to avoid the thermal injury associated with lasers. However, very limited data are available on its pre-clinical efficacy and safety. The novel robotic, fractional micro-coring device, AimeTM , was tested on four pigs, each treated in 12 sites, at 6 time-points, over the course of 28 days. Macroscopic and microscopic evaluation was performed at each of the 6 time-points during the 28-day follow-up...
January 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36683727/gene-expression-analysis-of-antioxidant-and-dna-methylation-on-the-rat-liver-after-4-week-wood-preservative-chromated-copper-arsenate-exposure
#23
JOURNAL ARTICLE
Naofumi Takahashi, Satoru Yamaguchi, Ryouichi Ohtsuka, Makio Takeda, Toshinori Yoshida, Tadashi Kosaka, Takanori Harada
Our previous 4-week repeated dose toxicity study showed that wood preservative chromated copper arsenate (CCA) induced hepatocellular hypertrophy accompanied by biochemical hepatic dysfunction and an increase in oxidative stress marker, 8-hydroxydeoxyguanosine, in female rats. To further explore the molecular mechanisms of CCA hepatotoxicity, we analyzed 10%-buffered formalin-fixed liver samples from female rats for cell proliferation, apoptosis, and protein glutathionylation and conducted microarray analysis on frozen liver samples from female rats treated with 0 or 80 mg/kg/day of CCA...
January 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36683726/application-of-convolutional-neural-network-for-analyzing-hepatic-fibrosis-in-mice
#24
JOURNAL ARTICLE
Hyun-Ji Kim, Eun Bok Baek, Ji-Hee Hwang, Minyoung Lim, Won Hoon Jung, Myung Ae Bae, Hwa-Young Son, Jae-Woo Cho
Recently, with the development of computer vision using artificial intelligence (AI), clinical research on diagnosis and prediction using medical image data has increased. In this study, we applied AI methods to analyze hepatic fibrosis in mice to determine whether an AI algorithm can be used to analyze lesions. Whole slide image (WSI) Sirius Red staining was used to examine hepatic fibrosis. The Xception network, an AI algorithm, was used to train normal and fibrotic lesion identification. We compared the results from two analyses, that is, pathologists' grades and researchers' annotations, to observe whether the automated algorithm can support toxicological pathologists efficiently as a new apparatus...
January 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36683725/two-cases-of-cartilaginous-metaplasia-in-the-sclera-of-japanese-white-rabbits
#25
Kotaro Yamada, Yoshinori Yamagiwa, Miki Masatsugu, Yu Haranosono
Spontaneous cartilaginous metaplasia of the sclera has not been reported in rabbits. Herein, we report two cases of spontaneous cartilaginous metaplasia in the sclera of Japanese White (JW) rabbits. Case 1 was noted in a 14-week-old male Kbs:JW rabbit that received a single ocular instillation of 20% isoproterenol (IP) a day before necropsy, and showed no abnormalities in clinical signs, ophthalmological assessments, and necropsy. Case 2 was noted in a 38-week-old male Kbs:JW rabbit that was housed under light-emitting diode (LED) lighting for 26 weeks and showed no effects of LED on clinical signs, ophthalmological assessments, and necropsy...
January 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36683724/effects-of-siraitia-grosvenorii-extract-on-nonalcoholic-steatohepatitis-like-lesions-in-sprague-dawley-rats-fed-a-choline-deficient-methionine-lowered-l-amino-acid-defined-diet
#26
JOURNAL ARTICLE
Kinuko Uno, Katsuhiro Miyajima, Shuji Ogawa, Noriko Suzuki-Kemuriyama, Dai Nakae
Siraitia grosvenorii is the fruit of a cucurbitaceous vine endemic to China. Its extract has been used as a sweetener and exhibits various anti-inflammatory and anticarcinogenic effects mediated via its antioxidant properties. In the present study, we aimed to clarify the preventive or ameliorative effects of S. grosvenorii extract (SGE) on nonalcoholic steatohepatitis-like lesions induced in male Hsd: Sprague Dawley rats fed a choline-deficient, methionine-lowered, l-amino acid-defined diet for 13 weeks. This diet increased hepatotoxicity parameters and upregulated the expression of inflammation- and fibrosis-related genes in the liver, resulting in the progression of hepatic lesions, oxidative stress, hepatocellular apoptosis, and fibrosis...
January 2023: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406175/inflammation-of-the-cardiac-coronary-artery-in-icr-mice
#27
Kyohei Yasuno, Masako Imaoka, Tetsuya Ohsawa, Keiko Okado, Kiyonori Kai, Yoshimi Tsuchiya
Inflammation of the cardiac coronary artery in ICR mice is occasionally observed in toxicity studies; however, this has not been well explored histologically. Herein, we investigated the detailed histology of the associated lesions in 6-8-week-old ICR mice. Coronary artery inflammation in the right ventricular wall was observed in 10 of 142 mice (7.0%). Histopathological examination revealed hypertrophy of the vascular smooth muscle cells and perivascular infiltration of macrophages in mild cases. In moderate to marked cases, single-cell necrosis of vascular smooth muscle cells, hemorrhage of the tunica media, and fibrinoid necrosis of the vessel wall were observed, in addition to the changes seen in mild cases...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406174/honorary-member-michihito-takahashi-m-d-ph-d-1937-2022
#28
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406173/lack-of-hepatocarcinogenicity-of-2-2-1-2-ethanediylbis-oxymethylene-bis-oxirane-3-hydroxy-2-naphthoic-acid-and-acetoacetanilide-in-a-medium-term-rat-liver-bioassay
#29
JOURNAL ARTICLE
Hiroshi Yamagata, Tsubasa Saito, Takezo Okamoto, Kensuke Satomoto, Tatsuya Mitsumoto, Atsushi Wakita, Maki Nakamura, Takahiro Hayashi, Yuichi Kuroiwa
The carcinogenicity of 2,2'-[1,2-ethanediylbis(oxymethylene)]bis-oxirane (ethylene glycol diglycidyl ether; EGDE), 3-hydroxy-2-naphthoic acid (HNA), and acetoacetanilide (AAA) was investigated using a medium-term rat liver bioassay for an occupational safety assessment. F344 male rats were administered a single intraperitoneal injection of diethylnitrosamine (200 mg/kg body weight (bw)/day) and then starting 2 weeks later, they received EGDE at 6, 20, and 60 mg/kg bw/day, HNA at 20, 60, and 200 mg/kg bw/day, or AAA at 60, 200, and 600 mg/kg bw/day by oral gavage for 6 weeks...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406172/kidney-organoid-derived-from-renal-tissue-stem-cells-is-a-useful-tool-for-histopathological-assessment-of-nephrotoxicity-in-a-cisplatin-induced-acute-renal-tubular-injury-model
#30
JOURNAL ARTICLE
Shota Ueno, Kenji Kokura, Yasushi Kuromi, Mitsuhiko Osaki, Futoshi Okada, Shinji Kitamura, Tetsuya Ohbayashi
Organoids derived from renal tissue stem cells (KS cells) isolated from the S3 segment of adult rat nephrons have previously been developed and evaluated. However, data regarding the histopathological evaluation of these organoids are limited. Therefore, in this study, we performed histopathological examinations of the properties of these organoids and evaluated the nephrotoxicity changes induced by cisplatin treatment. We observe that the tubular structure of the organoids was generally lined by a single layer of cells, in concordance with previous findings...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406171/early-detection-of-urinary-bladder-carcinogens-in-rats-by-immunohistochemistry-for-%C3%AE-h2ax-a-review-from-analyses-of-100-chemicals
#31
REVIEW
Takeshi Toyoda, Kumiko Ogawa
In safety evaluations of chemicals, there is an urgent need to develop short-term methods to replace long-term carcinogenicity tests. We have reported that immunohistochemistry for γ-H2AX, a well-established biomarker of DNA damage, can detect bladder carcinogens at an early stage using histopathological specimens from 28-day repeated-dose oral toxicity studies in rats. Given the markedly low level of γ-H2AX formation in the bladder urothelium of untreated rats, an increase in γ-H2AX-positive cells following chemical exposure can be relatively easy to identify...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406170/changes-in-5-fluorouracil-induced-external-granular-cell-damage-during-the-time-course-of-the-developing-cerebellum-of-infant-rats
#32
JOURNAL ARTICLE
Yuko Yamaguchi, Tsubasa Saito, Mizuho Takagi, Tomomi Nakazawa, Kazutoshi Tamura
5-Fluorouracil (5-FU) is widely used as a chemotherapeutic agent that blocks DNA synthesis and replication by inhibiting thymidylate synthetase. This study aimed to elucidate 5-FU-induced changes in the external granular cells (EGCs) in the cerebellum of infant rats and the possible underlying mechanism. Six-day-old infant rats were injected subcutaneously with 40 mg/kg of 5-FU, and their cerebellums were examined at 6, 9, 12, and 24 h after treatment (HAT), and 2, 4, and 10 d after treatment (DAT). The width of the external granular layer (EGL) decreased from 24 HAT to 4 DAT in the 5-FU group compared to that in the control group...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406169/primary-epididymis-squamous-cell-carcinoma-in-a-cb6f1-tg-rash2-mouse
#33
Manabu Ikeda, Toshihisa Fujiwara, Junko Fujishima, Airo Yukawa, Kinji Kobayashi
In a 26-week carcinogenicity study in rasH2-Tg mice, squamous cell carcinoma on the epididymis was observed in a male mouse in the positive control group treated with N-methyl-N-nitrosourea. A 29-week-old male rasH2-Tg mouse that was euthanized 21 weeks after the administration of N-methyl-N-nitrosourea had a white-grayish mass on the left caput epididymis. The mass was nodular and consisted of pleomorphic tumor cells forming alveolar, sheeted, and trabecular structures suggesting epithelial tumor growth. These cells presented a cobblestone-like arrangement and formed intercellular bridges...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406168/-in-vivo-screening-of-subcutaneous-tolerability-for-the-development-of-novel-excipients
#34
JOURNAL ARTICLE
Yuichi Takai, Stephanie Powlin, Yasuyuki Awasaki, Toshiaki Yamauchi, Tomoya Sano, Haruyuki Takahashi, Abdul-Hackam Ranneh, Yuta Arai
To develop safe subcutaneous formulations and minimize the risk of local irritation, it is essential to optimize the composition of active pharmaceutical ingredients and excipients. Depending on the physicochemical properties of the active pharmaceutical ingredient, additional excipients may be required to improve the stability and solubility of the active pharmaceutical ingredient. However, some of these excipients may not have been previously used in injectable drugs. Owing to the lack of safety data for such excipients, especially those used in subcutaneous dosing, it is important to evaluate their potential for local irritation during the early stages of formulation development...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/36406167/chemical-induced-lung-tumor-in-tg-rash2-mice-a-novel-mouse-tumor-model-to-assess-immune-checkpoint-inhibitors-combined-with-a-chemotherapy-drug
#35
JOURNAL ARTICLE
Teruaki Hagiwara, Takamasa Numano, Tomomi Hara, Taiki Sugiyama, Yukinori Mera, Seiko Tamano, Hiroto Miyata
In subcutaneous tumor models, changes in the tumor microenvironment can lead to differences in therapeutic treatment responses between the subcutaneous and parent tumors. Accordingly, we generated a lung carcinogenesis model that combines genetically modified mice (Tg-rasH2 mice) with two-stage chemical carcinogenesis as an alternative to the subcutaneous tumor model. In this model, Tg-rasH2 mice were treated with 1-ethyl-1-nitrosourea, followed by butylhydroxytoluene. Mice developed lung adenomas five weeks after treatment initiation...
October 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/35832903/the-potential-of-organoids-in-toxicologic-pathology-histopathological-and-immunohistochemical-evaluation-of-a-mouse-normal-tissue-derived-organoid-based-carcinogenesis-model
#36
REVIEW
Rikako Ishigamori, Mie Naruse, Akihiro Hirata, Yoshiaki Maru, Yoshitaka Hippo, Toshio Imai
Recently, we introduced an organoid-based chemical carcinogenesis model using mouse normal tissue-derived organoids. In the present review article, the histopathological and immunohistochemical characteristics of mouse normal tissue-derived organoids and tumors derived from these organoids after their in vitro treatment with genotoxic carcinogens and injection into nude mouse are reviewed. In organoids treated in vitro with genotoxic carcinogens, we confirmed macroscopic tumorigenicity and histopathological findings, including neoplastic characteristics, such as multilayered epithelia and/or invasion of epithelia into the surrounding interstitium...
July 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/35832902/a-case-of-spontaneous-rete-testis-adenoma-in-a-sprague-dawley-rat
#37
Masako Imaoka, Tetsuya Osawa, Kiyonori Kai, Yoshimi Tsuchiya
A 104-week-old male CD (SD) rat exhibited enlargement of the left testis. Microscopically, this mass was demarcated from the testis by fibrous connective tissue and characterized by cystic dilatation with single-layered columnar cells and papillary proliferation connected to the solid growth area without clear boundaries. In the solid growth area, cells were dissected into irregular alveolar nests by scant fibrous tissue with small blood vessels. The nuclei of proliferating cells were variable in size and round- to oval-shaped, and their cytoplasm was pale or eosinophilic and sometimes contained vacuoles or eosinophilic granules...
July 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/35832901/histopathology-of-acute-colchicine-intoxication-novel-findings-and-their-association-with-clinical-manifestations
#38
Shojiro Ichimata, Yukiko Hata, Kojiro Hirota, Naoki Nishida
A 32-year-old woman attempted suicide by ingesting Gloriosa bulbs and died approximately 2 days later. Toxicological examination revealed a potentially fatal blood concentration of colchicine (0.096 mg/L). In addition to the increased mitotic figures in the gastrointestinal mucosa, a unique finding for acute colchicine intoxication, pathological examination showed microvesicular lipid droplets in the liver, kidney, heart, and conduction system. Furthermore, central chromatolysis of neurons was observed in the pontine nucleus, medial accessory olivary nucleus, nucleus of the solitary tract, and nucleus ambiguus...
July 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/35832900/failure-to-gulp-surface-air-induces-swim-bladder-adenomas-in-japanese-medaka-oryzias-latipes
#39
JOURNAL ARTICLE
Satoshi Furukawa, Yuichiro Machida, Kazuya Takeuchi, Yumiko Hoshikawa, Kota Irie
In order to elucidate the effects of swim bladder inflation failure on swim bladder carcinogenesis, we investigated the sequential histopathological changes of swim bladders at 13, 24, 35, and 53 days post-hatch (dph) in medakas with an uninflated swim bladder, which was experimentally induced by denying access to the air-water interface between 0 and 6 dph. The reactive oxygen species (ROS) levels were measured at 24 dph. An uninflated swim bladder was induced in 47.3% of the fish denied access to the air-water interface (the denied group)...
July 2022: Journal of Toxicologic Pathology
https://read.qxmd.com/read/35832899/histopathologic-effect-of-in-ovo-exposure-to-methotrexate-at-early-embryonic-stage-on-optic-tectum-of-japanese-quail-coturnix-japonica
#40
JOURNAL ARTICLE
Masakatsu Nohara, Mayo Iwasaki, Mahiro Nishio, Akihiko Sugiyama
The optic tectum of Japanese quail embryos with in ovo exposure to methotrexate 100 ng/g egg on embryonic day 4 was examined from 3 to 24 hour after treatment. At 9 hour after methotrexate exposure, several apoptotic neuroepithelial cells appeared in the ventricular zone of the optic tectum; these increased in number and were diffusely distributed throughout all layers of the ventricular zone of the optic tectum at 12 hour. At 24 hour, neuroepithelial cells in the ventricular zone of the optic tectum were eliminated and showed sparse cell density...
July 2022: Journal of Toxicologic Pathology
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