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Experimental Dermatology

Xiaoping Yang, Aliaa E M Daifallah, Shiela Shankar, Jacob Beer, Christine Marshall, Tzvete Dentchev, Francesca Seykora, Sebastian D'Armas, Jaeyi Hahn, Vivian Lee, Hanan H Sabry, Assem M Farag, John T Seykora
Actinic keratoses (AKs) and squamous cell carcinoma in situ (SCCIS) are precursor lesions for cutaneous squamous cell carcinoma (cSCC), the second most common form of cancer. Current topical therapies for AKs and SCCIS promote skin inflammation to eradicate lesions and do not directly target the biological mechanisms driving growth. We hypothesized that topical small molecule inhibitors targeting kinases promoting keratinocyte growth in AKs and SCCIS could induce regression of these lesions with less inflammation...
February 14, 2019: Experimental Dermatology
Minh Binh Nguyen, Victor Julian Valdes, Idan Cohen, Venu Pothula, Dejian Zhao, Deyou Zheng, Elena Ezhkova
Merkel cells are mechanosensory cells involved in tactile discrimination. Merkel cells have been primarily studied in the murine back skin, where they are found in specialized structures called touch domes located around primary hair follicles. Yet, little is known about the morphogenesis of Merkel cells in areas of the skin devoid of hair, such as the glabrous paw skin. Here, we describe Merkel cell formation in the glabrous paw skin during embryogenesis. We first found in the glabrous paw skin that Merkel cells were specified at E15...
February 13, 2019: Experimental Dermatology
Ryosuke Saigusa, Takashi Yamashita, Shunsuke Miura, Megumi Hirabayashi, Kouki Nakamura, Takuya Miyagawa, Yuki Fukui, Ayumi Yoshizaki, Shinichi Sato, Yoshihide Asano
BACKGROUNDS: Stratified epithelia have caught much attention as potential contributors to the development of dermal and esophageal fibrosis in systemic sclerosis (SSc). Galectin-7 is a marker of all types of stratified epithelia, which is involved in the maintenance of epidermal homeostasis. So far, the role of galectin-7 has not been studied in SSc. OBJECTIVES: To investigate the potential contribution of galectin-7 to the development of clinical manifestations in SSc...
February 9, 2019: Experimental Dermatology
Ting-Xin Jiang, Hans I-Chen Harn, Kuang-Ling Ou, Mingxing Lei, Cheng-Ming Chuong
Wound-induced hair follicle neogenesis (WIHN) has been demonstrated in laboratory mice (Mus musculus) after large (>1.5 x 1.5 cm2 ) full-thickness wounds. WIHN occurs more robustly in African Spiny mice (Acomys cahirinus), which undergo autotomy to escape predation. Yet, the non-WIHN regenerative ability of the spiny mouse skin has not been explored. To understand the regenerative ability of the spiny mouse, we characterized skin features such as hair types, hair cycling, and the response to small and large wounds...
February 8, 2019: Experimental Dermatology
Yi Shan Lim, Duane P Harland, Thomas L Dawson
Human recorded history is littered with attempts to improve the perceived appearance of scalp hair. Throughout history treatments have included both biological and chemical interventions. Hair "quality" or "perceived appearance" is regulated by multiple biological intervention opportunities: adding more hairs by flipping follicles from Telogen to Anagen, or delaying Anagen follicles transiting into Catagen; altering hair "apparent amount" by modulating shaft diameter or shape; or, in principle, altering shaft physical properties changing its synthesis...
February 1, 2019: Experimental Dermatology
Kirsten Dietze-Schwonberg, Susanna Lopez Kostka, Beate Lorenz, Tommy Regen, Ari Waisman, Esther von Stebut
Proinflammatory IL-17 plays an important role in various diseases and defense against extracellular microorganisms. Healing of leishmaniasis is promoted by Th1/Tc1 cells, whereas Th2/Treg are associated with worsened disease outcome. In addition, high expression of IL-17A in Leishmania-susceptible BALB/c and artificial overexpression of IL-17A in T cells in resistant C57BL/6 mice worsened disease outcome. Since C57BL/6 mice lacking only IL-17A exhibited no phenotype, and IL-17A and IL-17F share similar receptors, but differentially regulate chemokine secretion, we studied mice lacking both IL-17A and IL-17F (IL-17A/F-/- ) in infections with Leishmania major...
January 31, 2019: Experimental Dermatology
Seung Hwan Paik, Soon-Jin Choi, Sunhyae Jang, Seong-Jin Jo, Kyu Han Kim, Ohsang Kwon
Hair follicle reconstitution requires highly organized epithelial-mesenchymal interactions. Skin equivalents containing the epidermal and dermal cells with hair reconstitution capacity can reproduce these processes, but have not been established. This study was conducted to develop a hair follicle-producing three-dimensional (3D) skin equivalent assay using neonate mouse epidermal and dermal cells. A skin equivalent comprised of mouse dermal cells (MDCs) embedded in type I collagen and overlaid with mouse epidermal cells (MECs) was used...
January 31, 2019: Experimental Dermatology
Hyun-Sun Park, Son Hye-Youn, Min-Ha Choi, Youngsook Son, Sundong Kim, Hyun-Sook Hong, Ji-Ung Park
There is an unmet need in novel therapeutics for atopic dermatitis (AD). We examined the effects of autologous adipose-derived stem cells (ADSCs) on AD-like skin lesions induced by the application of 2,4-dinitrochlorobenzene (DNCB) in NC/Nga mice. Autologous ADSCs and ADSC-conditioned medium (ADSC-CM) were injected intralesionally three times. Clinical severity and histopathologic findings were compared in sham naïve control, saline-treated, ADSC-treated, ADSC-CM-treated, and 2.5% cortisone lotion-applied animals...
January 28, 2019: Experimental Dermatology
Erin L Weber, Thomas E Woolley, Chao-Yuan Yeh, Kuang-Ling Ou, Philip K Maini, Cheng-Ming Chuong
Human skin progenitor cells will form new hair follicles, although at a low efficiency, when injected into nude mouse skin. To better study and improve upon this regenerative process, we developed an in vitro system to analyze the morphogenetic cell behavior in detail and modulate physical-chemical parameters to more effectively generate hair primordia. In this three-dimensional culture, dissociated human neonatal foreskin keratinocytes self-assembled into a planar epidermal layer while fetal scalp dermal cells coalesced into stripes, then large clusters, and finally small clusters resembling dermal condensations...
January 25, 2019: Experimental Dermatology
Ruifeng Liu, Wenjuan Chang, Juan Li, Yueai Cheng, Erle Dang, Xiaohong Yang, Qiang Wang, Gang Wang, Xinhua Li, Kaiming Zhang
Psoriasis is an autoimmune skin disease. Our previous studies revealed abnormal immune regulation of skin mesenchymal stem cells (S-MSCs) in psoriatic lesions. Circular RNA (circRNA) molecules were recently discovered as a new class of non-coding regulatory RNAs. Their role in the pathogenesis of psoriasis has not yet been studied. To explore potential circRNA-mediated mechanisms of S-MSCs in the pathogenesis of psoriasis, we sequenced mRNAs and circRNAs of MSCs from normal skin and psoriatic lesions, followed by functional prediction and interaction analyses...
January 21, 2019: Experimental Dermatology
Changyuan Wang, Jinbao Zong, Yongxi Li, Xiaoyan Wang, Weihua Du, Linfeng Li
Psoriasis is a chronic inflammatory disease and microRNAs have been reported to regulate the pathogenesis of psoriasis. Up-regulated miR-744-3p level was identified to associate with psoriasis while the precise functions of miR-744-3p in psoriasis were not well-elucidated. We first confirmed the up-regulation of miR-744-3p in psoriasis by measuring the its expression level in psoriatic samples. We explored the roles of miR-744-3p on keratinocytes proliferation and differentiation. We searched the targets of miR-744-3p and evaluated the roles of one target, KLLN on keratinocytes proliferation and differentiation...
January 21, 2019: Experimental Dermatology
Wen-Yen Huang, Edrick Tai-Yu Lin, Ya-Chieh Hsu, Sung-Jan Lin
Anagen hair follicle repair is the regenerative scheme activated to restore the structure and hair growth following injuries to anagen hair follicles. Compared with telogen-to-anagen regeneration and hair follicle neogenesis, anagen hair follicle repair is a clinically important, yet relatively unexplored regenerative feature of hair follicles. Due to their highly proliferative character, germinative cells and matrix cells within hair bulbs are highly susceptible to injuries, such as chemotherapy and radiotherapy...
January 21, 2019: Experimental Dermatology
Markus Böhm, Thomas Luger
Cutaneous wound healing is a complex process divided into different phases, i. e. an inflammatory, proliferative and remodelling phase. During these phases a variety of resident skin cell types but also cells of the immune system orchestrate the healing process. In the last year it has been shown that the majority of cutaneous cell types express the melanocortin 1 receptor (MC1R) that binds α-melanocyte-stimulating hormone (α-MSH) with high affinity and elicits pleiotropic biological effects, e. g. modulation of inflammation and immune responses, cytoprotection, antioxidative defence, and collagen turnover...
January 20, 2019: Experimental Dermatology
Julie Despres, Yasmina Ramdani, Marine di Giovanni, Magalie Bénard, Abderrakib Zahid, Maité Montero-Hadjadje, Florent Yvergnaux, Thibaut Saguet, Azeddine Driouich, Marie-Laure Follet-Gueye
It is well recognized that the world population is aging rapidly. Therefore, it is important to understand aging processes at the cellular and molecular levels to predict the onset of age-related diseases and prevent them. Recent research has focused on the identification of aging biomarkers, including those associated with the properties of the Golgi apparatus. In this context, Golgi-mediated glycosylation of proteins has been well characterized. Additionally, other studies show that the secretion of many compounds, including pro-inflammatory cytokines and extracellular matrix degrading enzymes, is modified during aging, resulting in physical and functional skin degradation...
January 19, 2019: Experimental Dermatology
R Kjaersgaard Andersen, H C Ring, K Kallenbach, J O Eriksen, G B E Jemec
BACKGROUND: The role of bacterial biofilm in hidradenitis suppurativa (HS) is highly debated. Less biofilm is found in clinically unaffected axillary perilesional skin of HS patients compared with healthy controls. OBJECTIVE: To study the correlation between biofilm and the phenotypical characterization of the pre-clinical inflammatory infiltrate. MATERIALS AND METHODS: An exploratory comparative study of punch biopsies from unaffected axillary HS skin compared to similarly biopsies from healthy controls underwent standard staining procedures for CD4, CD8, CD25, FoxP3 and IL17...
January 18, 2019: Experimental Dermatology
Agatha Stegemann, Markus Böhm
Tropisetron is a serotonin receptor (5-HT-R)-modulating agent and approved as an antiemetic for patients undergoing chemotherapy. In the gut it acts via specific serotonin receptors, 5-HT3 -R, to elicit its beneficial effects against nausea. We investigated whether tropisetron can affect inflammatory cell responses of human primary epidermal keratinocytes (NHK) which are key cells in the regulation of skin homeostasis. Tropisetron significantly and dose-dependently suppressed tumor necrosis factor (TNF)-α-mediated mRNA expression and protein secretion of interleukin (IL)-6 and IL-8 in these cells...
January 17, 2019: Experimental Dermatology
Stefano Colombo, Necati Harmankaya, Jorrit Jeroen Water, Adam Bohr
Rosacea is a prevalent skin condition dependent on the individual genetic profile. The current pharmacological management of this condition is mostly based on small molecule drugs predominately effective in ameliorating the inflammatory condition. Emerging molecular approaches could present an opportunity for managing rosacea conditions at transcriptomic level, and in the future allow personalized approaches. RNA medicines, such as small RNA interference (siRNA), could provide a flexible and applicable tool reaching this aim...
January 16, 2019: Experimental Dermatology
Jun Chen, Ke Liu, Yang Liu, Xue Wang, Zhen Zhang
Keloid is a dermal proliferative disorder characterized by the excessive proliferation and migration of keratinocytes and fibroblasts. Over-activation of the serine/threonine protein kinase, mammalian target of rapamycin (mTOR), plays a pivotal role in the process. Here, we show that both mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) were hyper-activated in keloid-derived primary keratinocytes. Further, OSI-027, an mTOR kinase inhibitor, potently inhibited proliferation and migration of keloid keratinocytes...
January 16, 2019: Experimental Dermatology
Bruno Méhul, Corinne Ménigot, Paul Fogel, Alexia Seraidaris, Alexandre Genette, Thierry Pascual, Madeleine Duvic, Johannes J Voegel
Stratum corneum collected by tape stripping from 10 and 24 subjects with Cutaneous T-Cell Lymphomas (CTCL) or psoriasis, respectively, were compared using quantitative label-free mass spectrometry analysis. A non-supervised statistical analysis (Posneg NMF) based on 352 differentially expressed proteins in both CTCL and psoriasis samples was able to separate the two disease groups and finally able to identify a set of 112 proteins that contributed most and significantly to the separation when compared to non-lesional samples...
January 13, 2019: Experimental Dermatology
F Beretti, L Bertoni, F Farnetani, C Pellegrini, G Gorelli, A M Cesinaro, L Reggiani Bonetti, L Di Nardo, S Kaleci, J Chester, C Longo, D Massi, M C Fargnoli, G Pellacani
Cutaneous melanoma (CM) is one of the most prevalent skin cancers, which lacks both a prognostic marker and a specific and lasting treatment, due to the complexity of the disease and heterogeneity of patients. Reflectance confocal microscopy (RCM) in vivo analysis is a versatile approach offering immediate morphological information, enabling the identification of four primary cutaneous RCM CM types. Whether RCM CM types are associated with a specific protein and molecular-genetic profiles at the tissue level, remains unclear...
January 12, 2019: Experimental Dermatology
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