journal
https://read.qxmd.com/read/38430031/astragaloside-iv-inhibits-idiopathic-pulmonary-fibrosis-through-activation-of-autophagy-by-mir-21-mediated-pten-pi3k-akt-mtor-pathway
#21
JOURNAL ARTICLE
Tiaohao Li, Xiaojuan Gao, Rui Jia, Yanling Sun, Yeli Ding, Fei Wang, Yujuan Wang
As the main active ingredient of Astragalus, Astragaloside IV (AS-IV) can ameliorate pulmonary fibrosis. In this experiment, we studied how AS-IV reduces idiopathic pulmonary fibrosis (IPF). Bleomycin (BLM) or TGF-β1 was treated in mice or alveolar epithelial cells to mimic IPF in vivo as well as in vitro. ASV-IV alleviated levels of inflammatory cytokines and fibrosis markers in IPF model. Through detection of autophagy-related genes, ASV-IV was observed to induce autophagy in IPF. Besides, ASV-IV inhibited miR-21 expression in IPF models, and overexpression of miR-21 could reverse the protective potential of ASV-IV on IPF...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430030/determination-of-volatile-aroma-composition-of-some-hazelnut-varieties-grown-in-ak%C3%A3-akoca-conditions
#22
JOURNAL ARTICLE
Hulya Unver, Yalcin Gucer, Ozan Haliscelik, Ebru Sakar, Zeynep Mujde Sakar, Masoumeh Khanahmadi, Sezai Ercisli
Türkiye is leading country for hazelnut production and hazelnut orchards are widely established in Black Sea belt. Akçakoca district belongs to Duzce province is accepted as one of the most important production areas. In this research, volatile aroma components in nuts (kernels) of Çakıldak, Kara, Sarı (Mincane), Tombul and Yomra hazelnut varieties grown in Akçakoca district were determined by SPME/GC-MS. The analysis revealed the presence a total of 55 different volatile aroma components...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430029/circ-ldlrad3-mir-655-3p-mapk1-axis-enhances-cell-migration-and-invasion-in-papillary-thyroid-carcinoma
#23
JOURNAL ARTICLE
Bingfei Zhang, Yiquan Peng, Lirong Zhou
Papillary thyroid carcinoma (PTC) is a prevalent histological subtype of thyroid cancer, whose occurrence and development may be related to circRNA dysregulation. This research proposed to unravel circ-LDLRAD3-related mechanisms in PTC. First, circ-LDLRAD3, miR-655-3p .and MAPK1 levels in PTC were quantitatively measured. Then, plasmid vectors or oligonucleotides that interfere with circ-LDLRAD3, miR-655-3p, or MAPK1 were transfected into PTC cells, followed by the analysis of proliferation, apoptosis, migration, and invasion...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430028/declined-circular-rna-mitofusin-2-constrains-the-deterioration-of-wilms-tumor-via-modulating-microrna-372-3p-transforming-growth-factor-%C3%AE-receptor-type-2-axis
#24
JOURNAL ARTICLE
Maolin Xiao, Xiaofeng Pu, Wei Tong, Xiao Xiao
To explore the action and mechanism in which circular RNA (circRNA) mitofusin 2 (MFN2) repressed the malignant proliferation of Wilms tumor (WT) via modulating microRNA (miR)-372-3p/transforming growth factor-β receptor type 2 (TGFBR2) axis. CircRNA MFN2 was distinctly elevated in the tissues and cells of WT patients, while miR-372-3p was silenced in the tissues and cells of WT. Test of TGFBR2, PCNA and Bax was implemented. Transfection with si-circRNA MFN2 or miR-372-3p-mimic restrained cancer cell advancement and the number of PCNA content was declined, while transfection with miR-372-3p-inhibitor was opposite, and PCNA content was augmented...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430027/effect-of-liraglutide-combined-with-jinlida-granules-on-glycolipid-metabolism-and-islet-function-of-type-2-diabetes-mellitus
#25
JOURNAL ARTICLE
Xianjuan Wang, Kun He, Jing Li, Ying Liu, Zhaokang Peng, Lijuan Zhao, Shumin Ji
To investigate whether Liraglutide combined with Jinlida granules affects glycolipid metabolism and islet function in the treatment of type 2 diabetes mellitus (T2DM), a control group and an observation group were established with 90 T2DM patients. The control group was given Jinlida treatment and the observation group was given liraglutide combined treatment for 12 weeks. The clinical efficacy, glycolipid metabolism, bone metabolism, islet function, and endothelial function. The curative effect of the observation group was better than that of the control group...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430026/circscube3-promoted-ferroptosis-to-inhibit-lung-adenocarcinoma-progression
#26
JOURNAL ARTICLE
Hongye Fu, Qiong Zhao
CircRNAs can regulate ferroptosis and affect cancer development and are promising biomarkers and therapeutic targets in lung cancer. circSCUBE3 is expressed in lung adenocarcinoma (LUAD) tissues. In this study, our purpose was to study the role and regulatory mechanism of circSCUBE3 in LUAD ferroptosis. circSCUBE3 was identified to be significantly downregulated in LUAD samples and cell lines. The expression of biomarkers related to lipid oxidation (4-HNE) and ferroptosis (Ptgs2) was both downregulated in LUAD tissues, suggesting the ferroptosis resistance in LUAD...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430025/combination-therapy-application-of-abemaciclib-with-doxorubicin-in-triple-negative-breast-cancer-cell-line-mda-mb-231
#27
JOURNAL ARTICLE
Tugce Nur Eralp, Akin Sevinc, Banu Mansuroglu
Due to lack of clinical biomarkers, Triple Negative Breast Cancer (TNBC) is more likely to have spread to other tissues at time of diagnosis and therapy planning generally involves use of cytotoxic chemotherapy agents, such as Doxorubicin. We aimed to investigate possible advantages of using combination strategy using Doxorubicin alongside Abemaciclib. After determining the IC50 values for Doxorubicin (DOX) and Abemaciclib (ABE); CompuSyn and ComBenefit software were used to reveal the effect resulting from the combination of two drugs...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430024/tetrandrine-alleviates-inflammation-and-promotes-macrophage-m2-polarization-in-gouty-arthritis-by-nf-%C3%AE%C2%BAb-mediated-lcp1
#28
JOURNAL ARTICLE
Li Fang, Rong Shen, Yao Lu, Xiangfeng Xu, Fang Huang
Gouty arthritis (GA) is an inflammatory disease caused by the deposition of monosodium urate (MSU) crystals into joints. Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from the root of Stephania tetrandra and can exert an anti-inflammatory function in different diseases. Nevertheless, the specific function of TET in GA remains unclear. We established the GA mouse model by MSU injection into joints of mice. Paw volume and gait score were detected for measuring the degree of joint swelling and the situation of joint dysfunction...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430023/mitochondria-targeted-reactive-oxygen-species-blockor-ss-31-blocks-hepatic-stellate-cell-activation-and-alleviates-hepatic-fibrosis-by-regulating-nlrp3-inflammasomes
#29
JOURNAL ARTICLE
Li Liu, YingFeng Wei, NingSheng Xie, Hong Cai, Ye Lin
This study aimed to elucidate the effect of mitochondria-targeted reactive oxygen species (ROS) blockor SS-31 on hepatic stellate cells (HSC) activation during liver fibrosis. TGF-β1 was employed to induce HSC activation, while MitoSOX Red was utilized to assess the presence of mitochondrial ROS. The mitochondrial membrane potential (MMP) was measured using the JC-1 probe, and the ATP level was determined using a specific kit. The proliferation of HSCs was assessed using CCK-8 and colony formation assays, whereas flow cytometry was employed to detect HSC apoptosis...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430022/homocysteine-modulates-cxcl10-cxcr3-axis-activity-to-induce-endothelial-dysfunction
#30
JOURNAL ARTICLE
Yanjie Xu, Yingying Xu, Zuozong Yu, Dingwen He
Elevated homocysteine (Hcy) levels have been linked to the development of cardiovascular diseases, notably endothelial dysfunction, a critical precursor to atherosclerosis. In this extensive investigation, we explore the intricate pathways through which Hcy influences endothelial dysfunction, with particular attention to the CXCL10/CXCR3 axis. Employing a dual approach encompassing both in vitro and in vivo models, we scrutinize the repercussions of Hcy exposure on endothelial functionality. Our results reveal that Hcy significantly impairs crucial endothelial processes, including cell migration, proliferation, and tube formation...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430021/dynamic-changes-and-clinical-value-of-sirt6-in-acute-coronary-syndrome-acs-patients
#31
JOURNAL ARTICLE
Gang Tian, Chunxiao Zhang
This study aimed to investigate the role of Sirt6 and inflammatory cytokines in blood samples of patients with ACS. This is a retrospective randomized controlled clinical trial, a total of 30 patients from our hospital are included and divided into following two groups: control group and experimental group, and experimental group consists of 15 patients with ACS and control group consists of 15 patients with non-acute coronary syndrome. Sirt6 protein is detected by western blotting and Sirt6 mRNA is detected by real-time PCR, then inflammatory cytokines such as IL-1β, IL-18, TnI, and CK-MB are measured by ELISA and cytokines NT-proBNP are monitored by immunofluorescence...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430020/mir-127-5p-regulates-faim2-mediated-cell-apoptosis-and-participates-in-cerebral-ischemia-reperfusion-injury
#32
JOURNAL ARTICLE
Teng Hu, Yongzhong Lin
Mechanical thrombectomy (MT) has become an effective re-airway method for cerebral ischemia-reperfusion injury (CI/RI). However, at present, there are few studies on the impact of MT therapy on the prognosis of CI/RI patients at home and abroad. Therefore, this paper aims to analyze the relevant factors affecting the prognosis of CI/RI patients after MT therapy. The main regulatory miRNAs during CI/RI in patients with MT were screened and studied. Serums were obtained from 80 patients (moderate to severe stroke) who underwent MT...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430019/effects-of-leukocyte-rich-platelet-rich-plasma-and-leukocyte-poor-platelet-rich-plasma-on-cartilage-in-a-rabbit-osteoarthritis-model
#33
JOURNAL ARTICLE
Kefan Zhang, Chenchen Zhang, Qiang Ren, Dening Wang, Li Sun, Xin Wang, Yaomin Wang, Hui Shi
Osteoarthritis is a prevalent chronic disease. One of its primary pathological processes involves the degeneration of articular cartilage. Platelet-rich plasma (PRP) contains cytokines and growth factors that can stimulate the repair and regeneration of articular cartilage tissues. PRP may also slow the progression of osteoarthritis. The purpose of this experiment is to compare the efficacy of Leukocyte poor (LP) - PRP and Leukocyte rich (LR) - PRP in treating rabbit osteoarthritis and to investigate their mechanisms of action...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430018/changes-in-biochemical-and-sperm-parameters-of-rats-drinking-energy-drinks
#34
JOURNAL ARTICLE
Sarwar N Jafar
Tiger energy drink is used in this research to see the effect on serum lipid profile, liver enzymes, and kidney function, also observing changes in sperm parameters. Sixteen male rats were divided into four groups, each consisting of four rats. The control group was given water and rat feed, while groups 1, 2, and 3 were given water with Tiger energy drink (25%, 50%, and 75%, respectively) for eight weeks. The results showed that the effect of energy drinks on lipid profile decreased cholesterol, TG, and LDL, while there was a slight increase in HDL...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430017/effects-of-exosomes-derived-from-platelet-rich-plasma-on-osteogenic-differentiation-of-dental-pulp-stem-cells
#35
JOURNAL ARTICLE
Chuzi Mo, Zhongjun Liu, Yunhe Lin, Nu Er Bi Ya A Bu Du Xi Ku, Siwei Li, Qiao Ruan, Chengxia Liu, Shuaimei Xu, Jun Wen
Platelet-rich plasma (PRP) can cause osteogenic differentiation of dental pulp stem cells (DPSCs). However, the effect of exosomes derived from PRP (PRP-Exos) on osteogenic differentiation of DPSCs remains unclear. Herein, we evaluated the impact of PRP-Exos on osteogenic differentiation of DPSCs. PRP-Exos were isolated and identified by transmission electron microscopy (TEM) and western blotting (WB). Immunofluorescence staining was performed to evaluate endocytosis of PRP-Exos by DPSCs. Alkaline phosphatase staining, alizarin red staining, western blot and qRT-PCR were carried out to evaluate the DPSCs osteogenic differentiation...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430016/effects-of-jerusalem-artichoke-enriched-diet-on-water-quality-growth-performance-feed-utilization-proximate-body-composition-and-hematology-and-biochemical-parameters-in-common-carp-fingerlings
#36
JOURNAL ARTICLE
Samad S Omar
The purpose of this research was to evaluate the impact of Jerusalem artichoke tubers (Helianthus tuberosus L.), a natural prebiotic, on the growth performance, proximate body composition, feed utilization, hematology, and biochemical parameters in common carp (Cyprinus carpio) fingerlings. Four JA-supplemented diets were formulated at 0.0% (control), 0.5%, 1%, and 2%. Fish were reared for ten weeks in cages placed in concrete ponds. Based on the results, an increase in the levels of JA supplementation led to significant improvements in growth and feed parameters (P<0...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430015/sema3a-ameliorates-the-pathological-progression-of-osteoarthritis-by-modulating-mitochondrial-damage-in-chondrocytes
#37
JOURNAL ARTICLE
Zhongkui Guo, Yong Qin, Yi Li, Peifu Tang
Osteoarthritis (OA) is a major disease that causes disability in middle-aged and elderly people. A comprehensive understanding of its pathogenesis is of great significance in finding new clinical diagnosis and treatment schemes. The role of Semaphorin 3A (Sema3A) in OS has attracted attention recently, and the purpose of this study is to analyze the mechanisms underlying its impact on OS. First, a rat model of OS was established. Hematoxylin-eosin (HE) and TUNEL staining showed that the modeled rats presented typical pathological manifestations of OS, confirming the success of the modeling...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430014/transcriptional-analysis-of-oxidative-and-nitrosative-stress-on-oral-opportunistic-candida-albicans
#38
JOURNAL ARTICLE
Rezhin Ali Mohammed, Karzan Abdulmuhsin Mohammad
The yeast Candida albicans is one of the most aggressive opportunistic pathogens in immunocompromised patients. The ability of the yeast to withstand stresses and radicals is of great concern. In the present study, four isolates of C. albicans were taken from patients with oral candidiasis and grown on RPMI for 24 hours at 37°C. Then, they were exposed to various concentrations of oxidative (H2O2) and nitrosative (HNO3) stress for two hours, and gene expression rates were measured through RT-PCR. After initial biofilm formation steps and growth validation, RNA extracted from the yeast and gene expression status were evaluated...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430013/quercetin-and-tanshinone-prevent-mitochondria-from-oxidation-and-autophagy-to-inhibit-kgn-cell-apoptosis-through-the-sirt1-sirt3-foxo3a-axis
#39
JOURNAL ARTICLE
Qingzhi Wan, Lisha Tang, Kaili Jin, Xuanyi Chen, Yinghuan Li, Xiaofeng Xu
Granulosa cells are somatic cells located inside follicles that play a crucial role in the growth and development of follicles. Quercetin and tanshinone are two key monomers in traditional Chinese medicine that have antioxidant and anti-aging properties. The KGN cell apoptosis model caused by triptolide (TP) was employed in this work to investigate granulosa cell death and medication rescue. Quercetin and tanshinone therapy suppressed KGN cell death and oxidation while also regulating the expression of critical apoptosis and oxidation-related markers such as B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax)...
February 29, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38430012/the-role-of-mir96-in-predicting-ctc-status-and-prognostic-evaluation-in-gastric-cancer-patients
#40
JOURNAL ARTICLE
Qi Zheng, Haiyan Ge
In this study, 20 patients clinically diagnosed with advanced gastric cancer were selected as subjects. Circulating tumor cells (CTCs) in the peripheral blood of gastric cancer patients were detected and counted by collecting peripheral blood samples at Ningbo No. 2 Hospital and using the Cell Rich TM system combined with a negative enrichment strategy. In addition, routine pathological examination and immunohistochemical staining were performed on surgical specimens, including HER2, EBER, E-cadherin, and vimentin indicators...
February 29, 2024: Cellular and Molecular Biology
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