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Cellular and Molecular Biology

Li-Xin Wu, Xiao-Feng Li, Hua-Fei Chen, You-Cai Zhu, Wen-Xian Wang, Chun-Wei Xu, Dong-Fang Xie, Yang Wan, Kai-Qi Du
This study aimed to systematically evaluate the value of combined detection of serum CEA and CA125 concentrations for the diagnosis of lung cancer. Related studies regarding the diagnosis of lung cancer were searched in PubMed, Embase, CNKI, and Wanfang using a computer. The number of patients who were true-positive, false-positive, false-negative, and true-negative were extracted from each study. Meta-analysis was performed using the Meta-Disc l.4, RevMan 5.3. Seven studies involving 2,216 cases were finally included...
December 31, 2018: Cellular and Molecular Biology
Ammad Ahmad Farooqi, Rukset Attar, Muhammad Zahid Qureshi, Sundas Fayyaz, Muhammad Imran Sohail, Uteuliyev Yerzhan Sabitaliyevich, Sadykov Bolat Nurmurzayevich, Armida Yelekenova, Ilhan Yaylim, Nada Alaaeddine
Based on the exciting insights gleaned from decades of ground-breaking research, it has become evident that deregulated signaling pathways play instrumental role in cancer development and progression. Interestingly discovery of non-coding RNAs has revolutionized our understanding related to transcription, post-transcription and translation. Modern era has witnessed landmark discoveries in the field of molecular cancer and non-coding RNA biology has undergone tremendous broadening. There has been an exponential growth in the list of publications related to non-coding RNAs and overwhelmingly increasing classes of non-coding RNAs are adding new layers of complexity to already complicated nature of cancer...
December 31, 2018: Cellular and Molecular Biology
Haoning Wang, Guanglin Cui, Xiaolong Wang, Minghai Zhang, Jimin Tan, Xingguang Li
To investigate the codon usage patterns of all available VP1 gene sequences of the GII.2 genotype, to determine the factors that affect these patterns, and to provide comprehensive details of the characteristics and evolution of the gene. Complete 519 sequences of VP1 gene of the HuNoV GII.2 genotype with known sampling dates and geographic locations from 1971 - 2017 were retrieved from the GenBank nucleotide database of the National Center for Biotechnology Information (NCBI) and analyzed. The percentage composition of T, C, A, and G nucleotides were 24...
December 31, 2018: Cellular and Molecular Biology
Fei Zhao, Chu-Wen Chen, Wen-Wen Yang, Li-Hua Xu, Zhi-Hao Du, Xi-Yuan Ge, Sheng-Lin Li
Circular RNAs(circRNA) are recently demonstrated to have a close relationship with tumors.To investigate the role of circular RNA in the pathogenesis of salivary adenoid cystic carcinoma(SACC), ten SACC tissues and paired normal submandibular gland(SMG) tissues were collected as the tumor group and the control group. Total RNA was extracted and then measured using ceRNA microarray (including mRNA, lncRNA, and circRNA) and miRNA microarray. Gene Ontology(GO) analysis and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathway analysis were performed in order to investigate the function of the differential expressing genes...
December 31, 2018: Cellular and Molecular Biology
Hakan Avci, Elif Sinem Iplik, Levent Aydemir, Senol Acar, Erkan Kiyak, Meral Unur, Bedia Cakmakoglu
Oral cavity cancers have anatomically a big part of the body system and include several types of cancer. The aim of the study is to investigate the relation between XPG and XPD gene variants in the DNA repair system and oral squamous cell cancers. A total of 111 patients with a pathologic diagnosis of oral squamous cell carcinoma and a control group of 148 healthy volunteers who presented to Istanbul Faculty of Medicine, Department of Otolaryngology & Head and Neck Surgery and Dentistry Faculty were included in the study...
December 31, 2018: Cellular and Molecular Biology
İbrahim Halil Yildirim, Nadir Koçak
Prevalence of neural tube defect (NTD) has reduced after folic acid intake. However; which mechanisms are effective in NTD are not known exactly. In this study; due to the possible effects on hypoxic pathway and embryonic development, particularly on extracellular matrix components, Hif-1α Pro582Ser and TGF-β3 IVS5+104 A/G SfaN1 polymorphisms were studied by PCR-RFLP method both on children with NTDs and mothers. Statistical differences were seen for Hif-1α and TGF-β3 IVS5+104 A/G SfaN1 polymorphisms in children with NTDs but no difference was seen in mothers...
December 31, 2018: Cellular and Molecular Biology
Yu Wang, Lan Zhao, Bohong Kan, Huiyan Shi, Jingxian Han
To investigate the expression of miR-22 in the hippocampus of amyloid β (1-42)-induced alzheimic rats, and to assess the underlying mechanism. A total of 60 male Sprague Dawley rats weighing between 274.65 and 293.97 g (mean weight = 284.31 ± 9.66 g) and aged 12 to 14 weeks were randomly assigned to three groups: control group (n = 20), Alzheimer's disease group (AD group; n = 20) and AD + miR-22 mimic group (ADMM group; n = 22). Rat AD model was established by injecting a solution of Aβ1-42 into the hippocampal CA1 regions...
December 31, 2018: Cellular and Molecular Biology
Elham Seidmohammadi, Saeed Abbasi, Mohammad Reza Asef
Twenty-five specimens of coprinoid fungi were collected during an ongoing survey of agaric fungi in Kermanshah Province, western Iran. The specimens were identified based on morphological characteristics and molecular analysis of internal transcribed spacer sequences. Five species of Coprinellus viz C. disseminates, C. flocculosus, C. micaceus, C. radians and C. xanthothrix, three species of Coprinopsis viz C. atramentaria, C. insignis and C. semitalis and two species of Coprinus viz C. pinetorum and C. sterquilinus were identified...
December 31, 2018: Cellular and Molecular Biology
Ozlem Ergul Erkec, Okan Arihan, Mehmet Kara, Ersin Karatas, Remzi Erten, Halit Demir, Ismail Meral, Muzaffer Mukemre, Fevzi Ozgokce
We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABAA) receptor positive cell number, cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats. Wistar albino rats were divided into 4 groups; Control, PTZ treated (PTZ), PTZ+L. leontopetalum extract treated (PTZ+LLE) and PTZ+B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ injection...
December 31, 2018: Cellular and Molecular Biology
Qiu-Ping Peng, De-Bing Du, Quan Ming, Fang Hu, Zhen-Bao Wu, Shaoqin Qiu
Acquired drug resistance is one of the main limitations in pharmacological therapy of malignancies including gastric cancer. MicroRNAs (miRNAs) are a class of small noncoding RNAs that suppress their targets by binding to the 3'UTR region of genes. In this study, we explored investigate the target gene of miR-494 and its roles in chemoresistance of gastric cancer. We found that miR-494 was significantly down-regulated in gastric cancer cells lines compared to the normal gastric epithelial cell line. Exogenous overexpression of miR-494 increased the chemosensitivity of gastric cancer cells to doxorubicin...
December 31, 2018: Cellular and Molecular Biology
Fang Han, Guoqiang Liu, Caifeng Sun, Jienan Wei
Multidrug resistance (MDR) poses a great impediment to cancer treatment. Excessive expression of ATP-binding cassette transport protein AC-1 (P-glycoprotein, P-GLP) is usually involved in MDR. In this study, ailanthone (AIL), a natural compound extracted from the whole seedlings of Ailanthus altissima (Simaroubaceae) was shown to mediate the reversal of P-GLP-induced MDR and restore the susceptibility of K562/A02 cells to doxorubicin (DOX). Further mechanistic studies revealed that AIL increased intracellular DOX accumulation and interrupted Rh123 efflux through suppression of P-GLP, and also suppressed P-GLP ATPase activity...
December 31, 2018: Cellular and Molecular Biology
Enes Akyüz, Pınar Mega Tiber, Merve Beker, Fahri Akbaş
Clinical and experimental studies show that epilepsy affects cardiac function; however, the underlying molecular mechanism has not been fully elucidated. Inwardly-rectifying potassium (Kir) channels transport K+ ions into excitable cells such as neurons and cardiomyocytes; they control the cell excitability by acting towards the repolarization phase of cardiac action potential. Kir channel expression has been previously shown to vary in epileptic brains, at the same time seizures are known to affect the autonomic nervous system...
December 31, 2018: Cellular and Molecular Biology
Elif Kaval Oğuz
Neurotrophic factors are growth factors that promote neuronal survival, regulate synaptic function and neurotransmitter release, and promote the plasticity and growth of axons in the peripheral and central nervous systems. This study focused on the roles of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in the survival of adult dorsal root ganglion (DRG) neurons following axotomy. To investigate this, we cultured adult mouse DRG neurons and administered NGF or BDNF to the culture medium at different doses before transection...
December 31, 2018: Cellular and Molecular Biology
Nadjet G Mestar, Malika N Boudiaf, Souad Lahcene, Hocine Abbaci, Ghenima I Aiche, Boussad Metna, Noria S Saadoun, Faiza Taibi
Many plant species produce phenolic compounds in their various organs and their use in crop protection. These plant secondary metabolites may serve as toxins against the insect pests. The objective of this study was to evaluate in vitro the bio-insecticidal effect of an aqueous extract of wild Olive leaves on Psylla larvae (Euphyllura olivina), a primary pest of the cultivated Olive tree (Olea europaea L. subsp sativa). Two concentrations of 0.05g/ml and 0.1g/ml leaves grinding powder in distilled water were sprayed on branches infested with Psylla larvae...
December 31, 2018: Cellular and Molecular Biology
Reza Hatami Moghadam, Amirhoushang Alvandi, Neda Akbari, Parvaneh Jafari, Ramin Abiri
Burn wound infection by A. baumannii is one of the predominant cause of mortality worldwide. The present investigation aimed at determination of antimicrobial resistance profile and expression of the biofilm-related genes in A. baumannii isolated from hospitalized patients with burn wound infection in Kermanshah hospitals. Sixty four isolates of A. baumannii were recovered from burn wound of hospitalized patients at hospitals in Kermanshah. The antimicrobial susceptibility testing (AST) was performed. Biofilm formation was measured and antibiotic resistance was compared between before and after of biofilm formation...
December 31, 2018: Cellular and Molecular Biology
Samira Ahmadipour, Isa Arji, Ali Ebadi, Vahid Abdossi
Water stress is one of the important abiotic environmental stresses that threaten the agricultural -products in the world. This experiment was carried out to determine the effect of water stress on physiological and biochemical characteristics of three commercial olive cultivars. A factorial pot experiment was conducted in the field conditions using completely randomized design in Gilan-Gharb city, Kermanshah province during 2015. One-year-old rooted cuttings of Zard, Amigdalolia and Konservolia olive cultivars were planted in 12-liter pots and subjected to three irrigation treatments...
December 31, 2018: Cellular and Molecular Biology
Hamzeh Mafakheri, Sayid Mahdi Mirghazanfari
The present study was conducted to assess the antifungal activity of essential oils of medicinal plants Mentha piperita (peppermint), Foeniculum vulgare, Satureja hortensis (Savory), Ferula asafoetida and Cuminum cyminum against Alternaria sp., Bipolaris sorokiniana and Acremonium sclerotigenum. The antifungal activity was evaluated by Broth Microdilution Method. Minimum Inhibitory Concentration (MICs) and Minimum Fungicidal Concentration (MFCs) of the essential oils were compared with Amphotricin B and Captan as standard drug...
December 31, 2018: Cellular and Molecular Biology
Nilufer Celik, Suleyman Aydin, Kader Ugur, Meltem Yardim, Mustafa Acet, Seyda Yavuzkir, İbrahim Sahin, Onder Celik
This study was planned to test whether follicular fluid (FF) levels of patatin-like phospholipase domain containing 3-gene (PNPLA3:adiponutrin), preptin, kisspeptin, and amylin change in polycystic ovarian syndrome (PCOS). A total of 40 infertile volunteers undergoing IVF/ICSI were included in the study. They were divided into two groups as PCOS (n=20) and control group without PCOS (n=20). The controls were recruited from subjects with a poor ovarian response. The PCOS and control participants were matched according to their body mass index (BMI)...
December 31, 2018: Cellular and Molecular Biology
Wancong Zhang, Linwang Tan, Yue Xing, Hanxing Zhao, Lungang Shi, Jianda Zhou, Xing Lang, Jianxiong Cai, Shijie Tang
To characterize the associations between the cleft palate (CPO) and single nucleotide polymorphisms (SNPs) of special AT-rich sequence-binding protein 2 (SATB2). We recruited 241 CPO and performed a case-control study with 242 controls. Concurrently, 103 of the patients and their normal parents were recruited to perform a case-parent trio study. Sixteen selected SNPs were genotyped. Furthermore, A meta-analysis was used to enhance the robustness of our conclusions. The case-control study provided no support for the hypothesis that any of the 16 selected SNPs played a significant role in CPO...
November 30, 2018: Cellular and Molecular Biology
Rimsha Farooq, Khadim Hussain, Shahid Nazir, Muhammad Rizwan Javed, Nazish Masood
CRISPR/Cas9 is a technology evolved from modified type II immune system of bacteria and archaea. Exploitation of this bacterial immune system in all eukaryotes including plants may lead to site-specific targeted genome engineering. Genome engineering is objectively utilized to express/silence a trait harbouring gene in the plant genome. In this review, different genetic engineering techniques including classical breeding, RNAi and genetic transformation and synthetic sequence-specific nucleases (zinc finger nucleases; ZFNs and transcription activator-like effector nuclease; TALENs) techniques have been described and compared with advanced genome editing technique CRISPR/Cas9, on the basis of their merits and drawbacks...
November 30, 2018: Cellular and Molecular Biology
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