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Faraday Discussions

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https://read.qxmd.com/read/31384874/dealing-with-complexity-general-discussion
#1
Carlos Afonso, Mark P Barrow, Nicholle G A Bell, Antony N Davies, Dumitru Duca, Cristian Focsa, Caroline Gauchotte-Lindsay, Pierre Giusti, Ruth Godfrey, Royston Goodacre, Jeffrey A Hawkes, Norbert Hertkorn, Jeroen J Jansen, Donald Jones, William Kew, Adrien Le Guennec, Anneke Lubben, Ljiljana Paša-Tolić, Ryan P Rodgers, Christopher P Rüger, Philippe Schmitt-Kopplin, Peter J Schoenmakers, Danilo Sciarrone, Stephen Summerfield, Dušan Uhrín, Fleur H M van Zelst
No abstract text is available yet for this article.
August 6, 2019: Faraday Discussions
https://read.qxmd.com/read/31380556/future-challenges-and-new-approaches-general-discussion
#2
Carlos Afonso, Caroline Chaux, Antony N Davies, Marc-André Delsuc, Francisco Fernandez Lima, Caroline Gauchotte-Lindsay, Pierre Giusti, Royston Goodacre, Jeffrey A Hawkes, Norbert Hertkorn, Jeroen J Jansen, William Kew, Stefan Kuhn, Anneke Lubben, Daniel McGill, Mathias Nilsson, John Parkinson, Ryan P Rodgers, Simon Rogers, Philippe Schmitt-Kopplin, Peter J Schoenmakers, Laetitia Shintu, Ronald Soong, Stephen Summerfield, Andrew Surman, Dušan Uhrín, Justin J J van der Hooft
No abstract text is available yet for this article.
August 5, 2019: Faraday Discussions
https://read.qxmd.com/read/31373341/data-mining-and-visualisation-general-discussion
#3
Carlos Afonso, Mark P Barrow, Antony N Davies, Marc-André Delsuc, Timothy Ebbels, Francisco Fernandez-Lima, Caroline Gauchotte-Lindsay, Pierre Giusti, Royston Goodacre, Norbert Hertkorn, Jeroen J Jansen, Donald Jones, William Kew, Stefan Kuhn, Adrien Le Guennec, Anneke Lubben, John Parkinson, Ljiljana Paša-Tolić, Simon Rogers, Timothy R Rudd, Peter J Schoenmakers, Philip Sidebottom, Stephen Summerfield, Gerjen H Tinnevelt, Gianluca Trifirò, Johan Trygg, Justin J J van der Hooft
No abstract text is available yet for this article.
August 2, 2019: Faraday Discussions
https://read.qxmd.com/read/31369012/high-resolution-techniques-general-discussion
#4
Elaine Adair, Carlos Afonso, Nicholle G A Bell, Antony N Davies, Marc-André Delsuc, Ruth Godfrey, Royston Goodacre, Jeffrey A Hawkes, Norbert Hertkorn, Donald Jones, Pedro Lameiras, Adrien Le Guennec, Anneke Lubben, Mathias Nilsson, Ljiljana Paša-Tolić, Josh Richards, Ryan P Rodgers, Christopher P Rüger, Philippe Schmitt-Kopplin, Peter J Schoenmakers, Philip Sidebottom, Dan Staerk, Stephen Summerfield, Dušan Uhrín, Pieter van Delft, Justin J J van der Hooft, Fleur H M van Zelst, Alexander Zherebker
No abstract text is available yet for this article.
August 1, 2019: Faraday Discussions
https://read.qxmd.com/read/31367716/high-throughput-protein-nanopatterning
#5
Xiangyu Liu, Mohit Kumar, Annalisa Calo', Edoardo Albisetti, Xiaouri Zheng, Kylie B Manning, Elisabeth Elacqua, Marcus Weck, Rein V Ulijn, Elisa Riedo
High-throughput and large-scale patterning of enzymes with sub-10 nm resolution, the size range of individual protein molecules, is crucial for propelling advancement in a variety of areas, from the development of chip-based biomolecular nano-devices to molecular-level studies of cell biology. Despite recent developments in bio-nanofabrication technology, combining 10 nm resolution with high-throughput and large-scale patterning of enzymes is still an open challenge. Here, we demonstrate a high resolution and high-throughput patterning method to generate enzyme nanopatterns with sub-10 nm resolution by using thermochemical scanning probe lithography (tc-SPL)...
August 1, 2019: Faraday Discussions
https://read.qxmd.com/read/31364656/multivalent-binding-of-concanavalin-a-on-variable-density-mannoside-microarrays
#6
Daniel J Valles, Yasir Naeem, Angelica Y Rozenfeld, Rawan W Aldasooky, Alexa M Wong, Carlos Carbonell, David R Mootoo, Adam B Braunschweig
Interactions between cell surface glycans and glycan binding proteins (GBPs) have a central role in the immune response, pathogen-host recognition, cell-cell communication, and a myriad other biological processes. Because of the weak association between GBPs and glycans in solution, multivalent and cooperative interactions in the dense glycocalyx have an outsized role in directing binding affinity and selectivity. However, a major challenge in glycobiology is that few experimental approaches exist for examining and understanding quantitatively how glycan density affects avidity with GBPs, and there is a need for new tools that can fabricate glycan arrays with the ability to vary their density controllably and systematically in each feature...
July 31, 2019: Faraday Discussions
https://read.qxmd.com/read/31339122/charge-tuning-of-glycosaminoglycan-based-hydrogels-to-program-cytokine-sequestration
#7
Uwe Freudenberg, Passant Atallah, Yanuar Dwi Putra Limasale, Carsten Werner
Glycosaminoglycan (GAG)-based biohybrid hydrogels of varied GAG content and GAG sulfation pattern were prepared and applied to sequester cytokines. The binding of strongly acidic and basic cytokines correlated with the integral space charge density of the hydrogel, while the binding of weakly charged cytokines was governed by the GAG sulfation pattern.
July 24, 2019: Faraday Discussions
https://read.qxmd.com/read/31338503/factors-contributing-to-variability-of-glycan-microarray-binding-profiles
#8
J Sebastian Temme, Christopher T Campbell, Jeffrey C Gildersleeve
Protein-carbohydrate interactions play significant roles in a wide variety of biological systems. Glycan microarrays are commonly utilized to interrogate the selectivity, sensitivity, and breadth of these complex protein-carbohydrate interactions. During the past two decades, numerous distinct glycan microarray platforms have been developed, each assembled from a variety of slide-surface chemistries, glycan-attachment chemistries, glycan presentations, linkers, and glycan densities. Comparative analyses of glycan microarray data have shown that while many protein-carbohydrate interactions behave predictably across microarrays, there are instances when various array formats produce different results...
July 24, 2019: Faraday Discussions
https://read.qxmd.com/read/31317169/binary-polymer-brush-patterns-from-facile-initiator-stickiness-for-cell-culturing
#9
Lina Chen, Peng Li, Xi Lu, Shutao Wang, Zijian Zheng
We report a new initiator stickiness method to fabricate micropatterned binary polymer brush surfaces, which are ideal platforms for studying cell adhesion behavior. The atom transfer radical polymerization (ATRP) initiator, ω-mercaptoundecyl bromoisobutyrate (MUDBr), is found to adsorb on several hosting polymer brushes, including poly[oligo(ethylene glycol)methyl ether methacrylate] (POEGMA), poly(2-hydroxyethyl methacrylate) (PHEMA), and poly(glycidyl methacrylate) (PGMA) brushes. Based on the initiator stickiness, micropatterned initiator molecules are printed onto a layer of homogenous hosting polymer brushes via microcontact printing (μCP), and then, vertically, a patterned second layer of polymer brushes is grown from the initiator areas...
July 18, 2019: Faraday Discussions
https://read.qxmd.com/read/31317165/systems-chemical-analytics-introduction-to-the-challenges-of-chemical-complexity-analysis
#10
Philippe Schmitt-Kopplin, Daniel Hemmler, Franco Moritz, Régis D Gougeon, Marianna Lucio, Markus Meringer, Constanze Müller, Mourad Harir, Norbert Hertkorn
Understanding complex (bio/geo)systems is a pivotal challenge in modern sciences that fuels a constant development of modern analytical technology, finding innovative solutions to resolve and analyse. In this introductory paper to the Faraday Discussion "Challenges in the analysis of complex natural systems", we aim to present concepts of complexity, and complex chemistry in systems subjected to biotic and abiotic transformations, and introduce the analytical possibilities to disentangle chemical complexity into its elementary parts (i...
July 18, 2019: Faraday Discussions
https://read.qxmd.com/read/31314021/probing-the-nanoscale-organisation-and-multivalency-of-cell-surface-receptors-dna-origami-nanoarrays-for-cellular-studies-with-single-molecule-control
#11
William Hawkes, Da Huang, Paul Reynolds, Linda Hammond, Matthew Ward, Nikolaj Gadegaard, John F Marshall, Thomas Iskratsch, Matteo Palma
Nanoscale organisation of receptor ligands has become an important approach to study the clustering behaviour of cell-surface receptors. Biomimetic substrates fabricated via different nanopatterning strategies have so far been applied to investigate specific integrins and cell types, but without multivalent control. Here we use DNA origami to surpass the limits of current approaches and fabricate nanoarrays to study different cell adhesion processes, with nanoscale spatial resolution and single-molecule control...
July 17, 2019: Faraday Discussions
https://read.qxmd.com/read/31313796/a-catch-and-release-receptor-for-the-cholera-toxin
#12
Clare S Mahon, Gemma C Wildsmith, Diksha Haksar, Eyleen de Poel, Jeffrey M Beekman, Roland J Pieters, Michael E Webb, W Bruce Turnbull
Stimuli-responsive receptors for the recognition unit of the cholera toxin (CTB) have been prepared by attaching multiple copies of its natural carbohydrate ligand, the GM1 oligosaccharide, to a thermoresponsive polymer scaffold. Below their lower critical solution temperature (LCST), polymers complex CTB with nanomolar affinity. When heated above their LCST, polymers undergo a reversible coil to globule transition which renders a proportion of the carbohydrate recognition motifs inaccessible to CTB. This thermally-modulated decrease in the avidity of the material for the protein has been used to reversibly capture CTB from solution, enabling its convenient isolation from a complex mixture...
July 17, 2019: Faraday Discussions
https://read.qxmd.com/read/31313794/biopolymer-monolith-for-protein-purification
#13
Yoshiko Miura, Hirokazu Seto, Makoto Shibuya, Yu Hoshino
Porous glycopolymers, "glycomonoliths", were prepared by radical polymerization based on polymerization-induced phase separation with an acrylamide derivative of α-mannose, acrylamide and cross-linker in order to investigate protein adsorption and separation. The porous structure was induced by a porogenic alcohol. The pore diameter and surface area were controlled by the type of alcohol. The protein adsorption was measured in both batch and continuous flow systems. The glycomonoliths showed specific interaction with the sugar recognition protein of concanavalin A, and non-specific interaction to other proteins was negligible...
July 17, 2019: Faraday Discussions
https://read.qxmd.com/read/31313786/engineering-of-spectator-glycocalyx-structures-to-evaluate-molecular-interactions-at-crowded-cellular-boundaries
#14
Daniel J Honigfort, Michelle H Zhang, Stephen Verespy, Kamil Godula
In the mucosal epithelium, the cellular glycocalyx can project tens to hundreds of nanometers into the extracellular space, erecting a physical barrier that provides protective functions, mediates the exchange of nutrients and regulates cellular interactions. Little is understood about how the physical properties of the mucosal glycocalyx influence molecular recognition at the cellular boundary. Here, we report the synthesis of PEG-based glycopolymers with tunable glycan composition, which approximate the extended architecture of mucin glycoproteins, and tether them to the plasma membranes of red blood cells (RBC) to construct an artificial mucin brush-like glycocalyx...
July 17, 2019: Faraday Discussions
https://read.qxmd.com/read/31309965/the-blind-men-and-the-elephant-challenges-in-the-analysis-of-complex-natural-mixtures
#15
Royston Goodacre
The identification of molecules from complex mixtures is difficult and full structure determination of the complete chemical milieu is yet to be achieved. Thus the comprehensive analysis of complex natural mixtures continues to challenge physical and analytical chemistry. Over the last 50 years or so, many research laboratories have strived to invent better analytical techniques with complementary physicochemical properties and improved resolving power, and to investigate upfront sample pre-treatments, which are necessary to enhance sample coverage from complex mixtures...
July 16, 2019: Faraday Discussions
https://read.qxmd.com/read/31305856/mimicking-the-endothelial-glycocalyx-through-the-supramolecular-presentation-of-hyaluronan-on-patterned-surfaces
#16
Xinqing Pang, Weiqi Li, Eliane Landwehr, Yichen Yuan, Wen Wang, Helena S Azevedo
The glycocalyx is the immediate pericellular matrix that surrounds many cell types, including endothelial cells (ECs), and is typically composed of glycans (glycosaminoglycans, proteoglycans, and glycoproteins). The endothelial glycocalyx is rich in hyaluronic acid (HA), which plays an important role in the maintenance of vascular integrity, although fundamental questions about the precise molecular regulation mechanisms remain unanswered. Here, we investigate the contribution of HA to the regulation of endothelial function using model surfaces...
July 15, 2019: Faraday Discussions
https://read.qxmd.com/read/31298259/hierarchically-patterned-striped-phases-of-polymerized-lipids-toward-controlled-carbohydrate-presentation-at-interfaces
#17
Tyson C Davis, Jeremiah O Bechtold, Tyler R Hayes, Terry A Villarreal, Shelley A Claridge
Complex biomolecules, including carbohydrates, frequently have molecular surface footprints larger than those in broadly utilized standing phase alkanethiol self-assembled monolayers, yet would benefit from structured orientation and clustering interactions promoted by ordered monolayer lattices. Striped phase monolayers, in which alkyl chains extend across the substrate, have larger, more complex lattices: nm-wide stripes of headgroups with 0.5 or 1 nm lateral periodicity along the row, separated by wider (∼5 nm) stripes of exposed alkyl chains...
July 12, 2019: Faraday Discussions
https://read.qxmd.com/read/31298252/multivalent-glycan-arrays
#18
Marco Mende, Vittorio Bordoni, Alexandra Tsouka, Felix F Loeffler, Martina Delbianco, Peter H Seeberger
Glycan microarrays have become a powerful technology to study biological processes, such as cell-cell interaction, inflammation, and infections. Yet, several challenges, especially in multivalent display, remain. In this introductory lecture we discuss the state-of-the-art glycan microarray technology, with emphasis on novel approaches to access collections of pure glycans and their immobilization on surfaces. Future directions to mimic the natural glycan presentation on an array format, as well as in situ generation of combinatorial glycan collections, are discussed...
July 12, 2019: Faraday Discussions
https://read.qxmd.com/read/31297494/magneto-and-photo-responsive-hydrogels-from-the-co-assembly-of-peptides-cyclodextrins-and-superparamagnetic-nanoparticles
#19
Benedikt P Nowak, Bart Jan Ravoo
Dual response to external non-invasive stimuli, such as light and magnetic field, is a highly desirable property in soft nanomaterials with potential applications in soft robotics, tissue engineering, and life-like materials. Within this class of materials, hydrogels obtained from the self-assembly of low molecular weight gelators (LMWGs) are of special interest due to their ease of preparation and modification. Herein, we report a modular co-assembly strategy for a magneto- and photo-responsive supramolecular hydrogel based on the arylazopyrazole (AAP) modified pentapeptide gelator Nap-GFFYS, and β-cyclodextrin vesicles (CDVs) with superparamagnetic cobalt ferrite nanoparticles embedded in their membranes...
July 12, 2019: Faraday Discussions
https://read.qxmd.com/read/31290873/going-large-r-general-discussion
#20
Musahid Ahmed, Steven Daly, Caroline Dessent, Otto Dopfer, Valérie Gabelica, Marie-Pierre Gaigeot, Michael Gatchell, Benny Gerber, Jemma Gibbard, Christopher Johnson, Mark Johnson, Kenneth Jordan, Anna Krylov, Kevin Lovelock, Martin Mayer, Laura McCaslin, Anne B McCoy, Daniel Neumark, Steen Brøndsted Nielsen, Jos Oomens, Anouk Rijs, Thomas Rizzo, Jana Roithová, Jack Simons, Mark Stockett, Adam Trevitt, Jan Verlet, Xue-Bin Wang, Roland Wester, Sotiris Xantheas
No abstract text is available yet for this article.
July 10, 2019: Faraday Discussions
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