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European Journal of Histochemistry: EJH

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https://read.qxmd.com/read/30739432/morphological-and-ultrastructural-analysis-of-normal-injured-and-osteoarthritic-human-knee-menisci
#1
Michela Battistelli, Marta Favero, Debora Burini, Giovanni Trisolino, Dante Dallari, Lucia De Franceschi, Steven R Goldring, Mary B Goldring, Elisa Belluzzi, Giuseppe Filardo, Brunella Grigolo, Elisabetta Falcieri, Eleonora Olivotto
The human meniscus plays a crucial role for transmission and distribution of load across the knee, as well as shock absorption, joint stability, lubrication, and congruity. The aim of this study was to compare the complex geometry, and unique ultrastructure and tissue composition of the meniscus in healthy (control) and pathological conditions to provide understanding of structural changes that could be helpful in the future design of targetted therapies and improvement of treatment indications. We analyzed meniscus samples collected from 3 healthy multi-organ donors (median age, 66 years), 5 patients with traumatic meniscal tear (median age, 41 years) and 3 patients undergoing total knee replacement (TKR) for end-stage osteoarthritis (OA) (median age, 72 years)...
February 11, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30678436/antigen-retrieval-pre-treatment-causes-a-different-expression-pattern-of-cav3-2-in-rat-and-mouse-spinal-dorsal-horn
#2
Xiao E Cheng, Long Xian Ma, Xiao Jin Feng, Meng Ye Zhu, Da Ying Zhang, Lin Lin Xu, Tao Liu
Cav3 channels consist of three isoforms, Cav3.1 (α1G), Cav3.2 (α1H), and Cav3.3 (α1I), which produce low-threshold spikes that trigger burst firings in nociceptive neurons of the spinal dorsal horn (SDH) and dorsal root ganglion (DRG). Although Cav3.2 plays a crucial role in pathological pain, its distribution in SDH still remains controversial. One study showed that Cav3.2 is ubiquitously expressed in neurons, but another study implied that Cav3.2 is expressed restricted to astrocytes. To unravel these discrepancies, we used methods of immunohistochemistry either with or without antigen retrieval (AR) pre-treatment to detect Cav3 in SDH and DRG from both rats and mice...
January 23, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30652437/sertoli-cells-methods-and-protocols
#3
CarloAlberto Redi
The mammalian somatic cell providing the physiological milieu to germ cells growth and differentiation is the Sertoli cell, named after the Italian physiologist Enrico Sertoli. Sertoli described for the first time this functionally multitasking cell in 1865 (when he was 23 years-old!) in human testicular tissues while ending up his medicine studies at the University of Pavia...
January 17, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30652436/epithelial-expression-of-the-hormone-leptin-by-bovine-skin
#4
Francesca Mercati, Cecilia Dall'Aglio, Ludovica Timperi, Paola Scocco, Elena De Felice, Margherita Maranesi
Leptin (Lep) stimulates keratinocytes to proliferate, intervenes in the wound healing and participates to hair follicle morphogenesis and cycle. While it is secreted by skin structures including epidermis and hair follicles, intradermal adipose tissue also seems to have a role in Lep secretion and accordingly in the control of hair follicle growth in mice and humans. Lep was investigated in the skin of humans and laboratory animals but there are not data regarding bovine species. The aim of this work was to study the expression of Lep and its receptor (LepR) in the skin of bovine and, at the same time, to investigate the presence and extension of intradermal adipose tissue...
January 17, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30652435/cell-migration-methods-and-protocols
#5
Manuela Monti
As the title suggests, this book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication...
January 17, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30652434/seasonal-expressions-of-prolactin-prolactin-receptor-and-stat5-in-the-scented-glands-of-the-male-muskrats-ondatra-zibethicus
#6
Wenqian Xie, Hong Liu, Qian Liu, Qiong Gao, Fuli Gao, Yingying Han, Zhengrong Yuan, Haolin Zhang, Qiang Weng
Prolactin (PRL) production in mammals has been demonstrated in extrapituitary gland, which can activate autocrine/paracrine signaling pathways to regulate physiological activity. In the current study, we characterized the gene expression profiles of PRL, prolactin receptor (PRLR) and signal transducers and activators of transcription 5 (STAT5) in the scented glandular tissues of the muskrats, to further elucidate the relationship between PRL and the scented glandular functions of the muskrats. The weight and volume of the scented glands in the breeding season were significantly higher than those of the non-breeding season...
January 17, 2019: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30572698/histochemistry-as-a-versatile-research-toolkit-in-biological-research-not-only-an-applied-discipline-in-pathology
#7
Carlo Pellicciari
The impressive progress of histochemistry over the last 50 years has led to setting up specific and sensitive techniques to describe dynamic events, through the detection of specific molecules in the very place where they exist in live cells. The scientific field where histochemistry has most largely been applied is histopathology, with the aim to identify disease-specific molecular markers or to elucidate the etiopathological mechanisms. Numerous authors did however apply histochemistry to a variety of other research fields; their interests range from the microanatomy of animal and plant organisms to the cellular mechanisms of life...
December 21, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30572697/two-histological-methods-for-recognition-and-study-of-cortical-microinfarcts-in-thick-sections
#8
Heiko Braak, Simone Feldengut, Jan Kassubek, Deniz Yilmazer-Hanke, Kelly Del Tredici
Cortical microinfarcts are the most widespread form of brain infarction but frequently remain undetected by standard neuroimaging protocols. Moreover, microinfarcts are only partially detectable in hematoxylin-eosin-stained (H and E) 4-10 µm paraffin sections at routine neuropathological examination. In this short report, we provide two staining protocols for visualizing cortical microinfarcts in 100-300 µm sections. For low-power microscopy, the first protocol combines aldehyde fuchsine staining for detection of lipofuscin granules and macrophages with Darrow red counterstaining for Nissl material...
December 20, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30572696/evolutionary-intraembryonic-origin-of-vertebrate-hematopoietic-stem-cells-in-the-elasmobranch-spleen
#9
Rosa Manca, Chester A Glomski, Alessandra Pica
The electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adulthood. Splenic development in this species was investigated and hemopoietic stem cells were detected in this organ by immunocytochemistry utilizing CD34 and CD38 antibodies. At stage I (2-cm-long embryos with external gills), the spleen contains only mesenchymal cells...
December 20, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30504933/epidermal-growth-factor-like-domain-multiple-7-egfl7-expression-and-possible-effect-on-biliary-epithelium-growth-in-cholangiocarcinoma
#10
Caterina L Mammola, Antonella Vetuschi, Luigi Pannarale, Roberta Sferra, Romina Mancinelli
Cholangiocarcinoma (CCA) is an aggressive biliary tract malignancy with limited treatment options and low survival rates. The intrahepatic subtype comprises two forms: mucin-iCCA and mixed-iCCA. Epidermal growth factor-like domain multiple (EGFL7) is overexpressed in less differentiated liver tumors. The aim of this study was to assess the presence of EGFL7 due to its possible role in the growth of CCA. Hematoxylin and Eosin and periodic acid-Schiff staining were used to evaluate the morphological aspects and glycogen deposition...
November 30, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30482005/dynamic-of-lipid-droplets-and-gene-expression-in-response-to-%C3%AE-aminoisobutyric-acid-treatment-on-3t3-l1-cells
#11
Monica Colitti, Federico Boschi, Tommaso Montanari
Research on adipobiology has recognized the browning process of white adipocytes as a potential therapeutic strategy for the treatment of obesity and related morbidities. Physical exercise stimulates the secretion of myokines, such as b-aminoisobutyric acid (BAIBA), which in turn promotes adaptive thermogenesis. White adipocyte conversion to brown cells involves dynamic changes in lipid droplet (LD) dimension and in the transcription of brown-specific marker genes. This study analyzes the effect of different doses of BAIBA and at different days of development on 3T3-L1 cells by evaluating morphological changes in LDs and the expression of browning gene markers...
November 28, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30465595/localization-of-cgrp-and-vegf-mrnas-in-the-mouse-superior-cervical-ganglion-during-pre-and-postnatal-development
#12
Kazuyuki Mitsuoka, Yoko Miwa, Takeshi Kikutani, Iwao Sato
The neuropeptide calcitonin gene-related peptide (CGRP) mediates inflammation and head pain by influencing the functional vascular blood supply. CGRP is a well-characterized mediator of receptor-regulated neurotransmitter release. However, knowledge regarding the role of CGRP during the development of the superior cervical ganglion (SCG) is limited. In the present study, we observed the localization of CGRP and vascular endothelial growth factor (VEGF-A) mRNAs during prenatal development at embryonic day 14...
November 22, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30426733/overexpression-of-kynurenic-acid-and-3-hydroxyanthranilic-acid-after-rat-traumatic-brain-injury
#13
Arturo Mangas, Margarita Heredia, Adelaida Riolobos, Antonio De la Fuente, José María Criado, Javier Yajeya, Michel Geffard, Rafael Coveñas
Using an immunohistochemical technique, we have studied the distribution of kynuneric acid (KYNA) and 3-hydroxyanthranilic acid (3-HAA) in a rat brain injury model (trauma). The study was carried out inducing a cerebral ablation of the frontal motor cortex. Two mouse monoclonal specific antibodies previously developed by our group directed against KYNA and 3-HAA were used. In control animals (sham-operated), the expression of both KYNA and 3-HAA was not observed. In animals in which the ablation was performed, the highest number of immunoreactive cells containing KYNA or 3-HAA was observed in the region surrounding the lesion and the number of these cells decreased moving away from the lesion...
November 14, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30418011/ultrastructural-histochemistry-in-biomedical-research-alive-and-kicking
#14
Manuela Malatesta
The high-resolution images provided by the electron microscopy has constituted a limitless source of information in any research field of life and materials science since the early Thirties of the last century. Browsing the scientific literature, electron microscopy was especially popular from the 1970's to 80's, whereas during the 90's, with the advent of innovative molecular techniques, electron microscopy seemed to be downgraded to a subordinate role, as a merely descriptive technique. Ultrastructural histochemistry was crucial to promote the Renaissance of electron microscopy, when it became evident that a precise localization of molecules in the biological environment was necessary to fully understand their functional role...
November 7, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30362673/regenerative-potential-of-the-bichat-fat-pad-determined-by-the-quantification-of-multilineage-differentiating-stress-enduring-cells
#15
Giamaica Conti, Dario Bertossi, Elena Dai Prè, Chiara Cavallini, Maria Teresa Scupoli, Giulia Ricciardi, Pierpaolo Parnigotto, Yves Saban, Andrea Sbarbati, Pierfrancesco Nocini
Published studies regarding Bichat fat pad focused, quite exclusively, on the implant of this adipose depot for different facial portions reconstruction. The regenerative components of Bichat fat pad were poorly investigated. The present study aimed to describe by an ultrastructural approach the Bichat fat pad, providing novel data at the ultrastructural and cellular level. This data sets improve the knowledge about the usefulness of the Bichat fat pad in regenerative and reconstructive surgery. Bichat fat pads were harvested form eight patients subjected to maxillofacial, dental and aesthetic surgeries...
October 23, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30362674/mouse-oocyte-development-methods-and-protocols
#16
Manuela Monti
The Springer Protocols series "Methods in Molecular Biology" has published its 1818th volume which is entirely devoted to the development of the female gamete: the oocyte.
September 26, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30362672/cytoplasmic-lattices-are-not-linked-to-mouse-2-cell-embryos-developmental-arrest
#17
Marianna Longo, Michele Boiani, CarloAlberto Redi, Manuela Monti
Cytoplasmic lattices are important regulators of oocyte maturation. They store components of the protein synthesis machinery including ribosomes and, among others, they are involved in the regulation of microtubule dynamics in both mouse and human. Cytoplasmic lattices undergo dramatic reorganizations at crucial stages of oocyte maturation, where they are abundantly present in the cytoplasm of developmentally competent oocytes named SN (Surrounded Nucleolus) while they are rare in the cytoplasm of 2-cell stage-arresting NSN (Not Surrounded Nucleolus) oocytes, suggestive of a requirement of cytoplasmic lattices for development past the 2-cell stage...
September 24, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30223640/study-of-liver-in-hbv-related-hepatocellular-carcinoma-stereology-shows-quantitative-differences-in-liver-structure
#18
Bita Moudi, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb
Hepatocellular carcinoma is one of the main consequences of liver chronic disease. Hepatocellular carcinoma-related changes may be seen in patients with chronic hepatitis B. The aim of the current study was to quantitate liver tissue elements by stereological technique in patients with hepatitis B-related cancer and compare the results with control and only hepatitis B group. Needle liver biopsies from 40 patients with only chronic hepatitis B infection, from 41 patients with only early hepatocellular carcinoma, from 40 patients with early hepatitis B-related cancer and 30 healthy subjects (control group) were analyzed by stereological method using systematic uniform random sampling method...
September 18, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30362671/cancer-stem-cell-markers-aldh1-and-cd44-cd24-phenotype-and-their-prognosis-impact-in-invasive-ductal-carcinoma
#19
Iris Rabinovich, Ana Paula Martins Sebastião, Rubens Silveira Lima, Cícero De Andrade Urban, Eduardo Schunemann Junior, Karina Furlan Anselmi, Selene Elifio-Esposito, Lucia De Noronha, Andréa Novais Moreno-Amaral
Breast cancer is a very heterogeneous disease. The intrinsic molecular subtypes can explain the intertumoral heterogeneity and the cancer stem cell (CSC) hypothesis can explain the intratumoral heterogeneity of this kind of tumor. CD44+/CD24- phenotype and ALDH1 expression are the major CSC markers described in invasive breast cancer. In the present study, 144 samples of invasive breast carcinoma, no special type were distributed in 15 tissue microarrays (TMA) and then evaluated for expression of the CD44+/CD24- phenotype and ALDH1 to understand the importance of these CSC markers and the clinical aspects of breast cancer...
September 3, 2018: European Journal of Histochemistry: EJH
https://read.qxmd.com/read/30176704/low-ozone-concentrations-promote-adipogenesis-in-human-adipose-derived-adult-stem-cells
#20
Manuela Costanzo, Federico Boschi, Flavia Carton, Giamaica Conti, Viviana Covi, Gabriele Tabaracci, Andrea Sbarbati, Manuela Malatesta
Ozone is a strong oxidant, highly unstable atmospheric gas. Its medical use at low concentrations has been progressively increasing as an alternative/adjuvant treatment for several diseases. In this study, we investigated the effects of mild ozonisation on human adipose-derived adult stem (hADAS) cells i.e., mesenchymal stem cells occurring in the stromal-vascular fraction of the fat tissue and involved in the tissue regeneration processes. hADAS cells were induced to differentiate into the adipoblastic lineage, and the effect of low ozone concentrations on the adipogenic process was studied by combining histochemical, morphometric and ultrastructural analyses...
September 3, 2018: European Journal of Histochemistry: EJH
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