Marilou Vandenhole, Xueping Lu, Dimitra Tsakireli, Catherine Mermans, Sander De Rouck, Berdien De Beer, Eba Simma, Spiros A Pergantis, Wim Jonckheere, John Vontas, Thomas Van Leeuwen
The molecular mechanisms of amitraz and chlorfenapyr resistance remain only poorly understood for major agricultural pests and vectors of human diseases. This study focusses on a multi-resistant field strain of the crop pest Tetranychus urticae, which could be readily selected in the laboratory to high levels of amitraz and chlorfenapyr resistance. Toxicity experiments using tralopyril, the active toxophore of chlorfenapyr, suggested decreased activation as a likely mechanism underlying resistance. Starting from the same parental strain, transcriptome profiling revealed that a cluster of detoxifying genes was upregulated after amitraz selection, but unexpectedly downregulated after chlorfenapyr selection...
November 20, 2023: Insect Biochemistry and Molecular Biology