Yuanyuan Liu, Sanyuan Ma, Xiaogang Wang, Jiasong Chang, Jie Gao, Run Shi, Jianduo Zhang, Wei Lu, Yue Liu, Ping Zhao, Qingyou Xia
Bombyx mori is an economically important insect and a model organism for studying lepidopteran and arthropod biology. Using a highly efficient CRISPR/Cas9 system, we showed that this system could mediate highly efficient targeted genome editing of a single gene locus, large chromosomal deletion or inversion, and also multiplex genome editing of 6 genes simultaneously in BmNs cell line derived from B. mori. The simplicity and high efficiency of our system provide unprecedented possibilities for researchers to implement precise and sophisticated manipulation of a chosen B...
June 2014: Insect Biochemistry and Molecular Biology