Margot A N Dode, Natalia Capobianco, Luna Nascimento Vargas, Bruna Mion, Nayara Ribeiro Kussano, José Felipe Spricigo, Mauricio Machaim Franco
PURPOSE: This study aimed to identify a marker for freezability and in vitro fertility of sperm samples before freezing. METHODS: Semen was collected from nine Nelore bulls; half of the ejaculate was used for seminal plasma cell-free DNA (cfDNA) quantification, and the other half was cryopreserved. Evaluation of sperm movement using computer-assisted semen analysis and plasma membrane integrity and stability, acrosomal integrity, apoptosis, and mitochondrial potential using flow cytometry were performed on fresh and frozen/thawed semen at 0, 3, 6, and 12 h after thawing...
March 5, 2024: Journal of Assisted Reproduction and Genetics