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Phytochemical Analysis: PCA

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https://read.qxmd.com/read/30891865/use-of-ion-mobility-mass-spectrometry-to-enhance-cumulative-analytical-specificity-and-separation-to-profile-6-c-8-c-glycosylflavone-critical-isomer-pairs-and-known-unknowns-in-medicinal-plants
#1
Michael McCullagh, Cintia Alessandra Matiucci Pereira, Janete Harumi Yariwake
INTRODUCTION: Plant medicine/herbal extracts are typically complex, encompassing a wide range of flavonoid diversity and biological benefits. Combined with a lack of standards; species authentication profiling is a challenge. A non-targeted screening strategy using two-dimensional (2D) separation and specificity of ultra-high-performance liquid chromatography ion mobility collision-induced dissociation mass spectrometry (UHPLC-IM-CID-MS) has been investigated, to identify the 6-C and 8-C-glycosylflavone isomer orientin/isoorientin and vitexin/isovitexin pairs in Passiflora species...
March 19, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30859657/searching-for-calcium-antagonists-for-hypertension-disease-therapy-from-moutan-cortex-using-bioactivity-integrated-uhplc-qtof-ms
#2
Yujie Lu, Yanfang Deng, Wenjuan Liu, Min Jiang, Gang Bai
INTRODUCTION: Calcium channel blockers (CCBs) are currently the most commonly used drugs for the treatment of hypertension. Moutan Cortex (MC), a traditional Chinese herb, has been found to have an anti-hypertensive effect. However, its potential mechanisms in the regulation of intracellular calcium concentration ([Ca2+ ]i ) remain poorly understood. OBJECTIVE: The main objective of this work was to identify the potential calcium antagonists from MC and study their molecular mechanisms...
March 11, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30816611/structural-characterisation-and-discrimination-of-the-aerial-parts-of-paris-polyphylla-var-yunnanensis-and-paris-polyphylla-var-chinensis-by-uhplc-qtof-ms-coupled-with-multivariate-data-analysis
#3
Meng-Yuan Liang, Yuan-Zhong Wang, Xin Qiao, Ya-Wen Lu, Mei-Hong Chen, Ping Li, Xiao-Dong Wen, Jie Yang
INTRODUCTION: As sources of Rhizoma Paridis are facing shortages, utilising the aerial parts of Paris polyphylla has emerged as a promising additional source. However, the components in the aerial parts still need to be explored, and it is difficult to distinguish the aerial parts of P. polyphylla Smith var. yunnanensis (PPY) and P. polyphylla var. chinensis (PPC), two varieties of P. polyphylla. OBJECTIVE: This study aimed to establish a comprehensive platform to characterise steroid saponins from the aerial parts of PPY and PPC and to discriminate these two varieties...
February 28, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30779265/a-novel-similarity-search-approach-for-high-performance-thin-layer-chromatography-hptlc-fingerprinting-of-medicinal-plants
#4
Heshmatollah Ebrahimi-Najafabadi, Seyed Sina Kazemeini, Ardalan Pasdaran, Azadeh Hamedi
INTRODUCTION: In addition to the development of analytical equipment, another movement has also appeared in the field of computer assisted techniques for metabolite assessment. Although, some studies can be found in the literature there is still not available reliable and user-friendly software which is coupled with a simple chromatography method for developing a database to identify medicinal plants. OBJECTIVES: Developing a novel similarity search approach for high-performance thin-layer chromatography (HPTLC) fingerprinting...
February 19, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30762270/evaluation-of-phenolic-compounds-from-viroid-free-and-viroid-infected-apples-using-hplc-pda-esi-ms-ms
#5
Thi Phuong Duyen Vu, In Sook Cho, Kyung Tae Kim, Seo Young Yang, Young Ho Kim, Jong Seong Kang
INTRODUCTION: Apples are the most consumed fruits in Korea and have played an economically important role in Korean agriculture. However, widespread viroid infection in apples had been reported in recent years. OBJECTIVES: The objective of this study was to clarify the relationships between viroid-disease resistance and phenols content of three apple cultivars: "Fuji", "Gamhong", and "Hongro". A model for distinguishing between viroid-free and viroid-infected apples was also developed...
February 14, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30762260/hr-lc-esi-orbitrap-ms-based-metabolite-profiling-of-prunus-dulcis-mill-italian-cultivars-toritto-and-avola-husks-and-evaluation-of-antioxidant-activity
#6
Alfredo Bottone, Milena Masullo, Paola Montoro, Cosimo Pizza, Sonia Piacente
INTRODUCTION: Prunus dulcis Mill. is a plant cultivated for the production of its edible seeds, known as sweet almonds. In the last decades almond demand has highly increased due to their wide use in the industrial manufacturing of almond-based foods; this has led to a huge production of waste materials, mainly corresponding to shells and husks. OBJECTIVE: To achieve a deeper understanding of the chemical composition of almond husks with the aim of exploring these byproducts as a source of bioactives to be used in nutraceutical and cosmetic formulations...
February 14, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30762258/classification-of-barberry-genotypes-by-multivariate-analysis-of-biochemical-constituents-and-hplc-profiles
#7
Naser Gholizadeh-Moghadam, Bahman Hosseini, Abolfazl Alirezalu
INTRODUCTION: Recently, there has been a growing interest in the use of edible barberry and their extracts as a source of natural antioxidants in food and pharmaceutical industries. OBJECTIVE: The aim of this study was to evaluate the biochemical constituents of 18 samples of barberry fruits and classification of barberry genotypes by multivariate analysis. METHODS: Total phenolic, total flavonoid, total anthocyanin, total tannin, total carbohydrate contents and antioxidant activity were determined using Folin-Ciocalteu, aluminum chloride, colorimetric, vanillin, anthron and DPPH (2,2'-diphenyl-1-picrylhydrazyl) assays, respectively...
February 14, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30687967/the-use-of-a-microwave-assisted-solvent-extraction-coupled-with-hplc-uv-pad-to-assess-the-quality-of-marrubium-vulgare-l-white-horehound-herbal-raw-material
#8
Emanuela Martino, Serena Della Volpe, Valeria Cavalloro, Bedis Amri, Leila Bettaeib Been Kaab, Giorgio Marrubini, Daniela Rossi, Simona Collina
INTRODUCTION: Marrubium vulgare is a herbal remedy presents in several European Pharmacopoeias and commonly marketed as white horehound. The chemotaxonomic marker of Marrubium genus is marrubiin and its content may change in response to biotic and abiotic stress. OBJECTIVE: Development of a microwave-assisted solvent extraction (MASE) methodology suitable for exhaustively extracting marrubiin from M. vulgare leaves, easily applicable to large sets of samples. Evaluation of the influence of copper(II) on marrubiin production...
January 27, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30648323/quantitative-analysis-of-phenolic-metabolites-in-copaifera-langsdorffii-leaves-from-plants-of-different-geographic-origins-cultivated-under-the-same-environmental-conditions
#9
Erick V S Motta, Bruno L Sampaio, Juliana C Costa, Simone P Teixeira, Jairo K Bastos
INTRODUCTION: Galloylquinic acid derivatives and flavonoids are the main phenolic metabolites found in Copaifera langsdorffii leaves (Leguminosae, Detarioideae), a medicinal plant with potential therapeutic application in the treatment of kidney stones. The factors that affect metabolite production in this plant species are not well understood but may include environmental and genetic factors. OBJECTIVE: To quantify the variation in metabolite production over a 12-month period for 10 groups of C...
January 15, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30644147/wood-and-roots-of-major-grapevine-cultivars-and-rootstocks-a-comparative-analysis-of-stilbenes-by-uhplc-dad-ms-ms-and-nmr
#10
Julien Gabaston, Cécile Leborgne, Pierre Waffo-Teguo, Josep Valls, Antonio Palos Pinto, Tristan Richard, Stéphanie Cluzet, Jean-Michel Mérillon
INTRODUCTION: Grapevine wood and roots are by-products obtained during vineyard management. This plentiful biomass is known to be rich in stilbenes and can be used as a source of high-value compounds as well as active natural extracts. However, the stilbenes in grapevine wood and roots from different cultivars and rootstocks remain to be characterized. OBJECTIVE: The present study investigated the stilbene content of eight major Vitis vinifera cultivars and six different rootstocks...
January 15, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30644146/in-depth-analysis-of-crocetin-ester-glycosides-from-dried-processed-stigmas-of-crocus-sativus-l-by-hplc-esi-ms-n-n-2-3
#11
Ernst Pittenauer, Edita Rados, Anthony Tsarbopoulos, Günter Allmaier
INTRODUCTION: Saffron stigmas from Crocus sativus L. (Iridaceae) are used as a drug in folk medicine, as a food additive and as a dying agent for at least 3500 years. Despite this long-term use the chemical composition of saffron seems still to be not fully known. OBJECTIVE: An analytical strategy for detailed investigations of aqueous saffron extract is developed based on reverse-phase high-performance liquid chromatography electrospray ionisation (HPLC-ESI) multistage mass spectrometry (MSn ) for crocins...
January 15, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30625513/total-polyphenol-quantitation-using-integrated-nir-and-mir-spectroscopy-a-case-study-of-chinese-dates-ziziphus-jujuba
#12
Muhammad Arslan, Zou Xiaobo, Haroon Elrasheid Tahir, Muhammad Zareef, Hu Xuetao, Allah Rakha
INTRODUCTION: Polyphenols are the foremost measure of phytochemicals in Chinese dates due to their many potential health benefits such as averting cancers, reducing the risk of coronary artery disease, diuretic activity, myocardial stimulant, coronary dilator and muscle relaxant. OBJECTIVE: To quantitate the polyphenols in Chinese dates using a data fusion approach with near-infrared (NIR) and mid-infrared (MIR) spectroscopy. MATERIAL AND METHODS: A total of 80 Chinese dates samples were used for data acquisition from both NIR and MIR spectroscopy...
January 9, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30609101/tlc-bioautography-identification-and-gc-ms-analysis-of-antimicrobial-and-antioxidant-active-compounds-in-musa-%C3%A3-paradisiaca-l-fruit-pulp-essential-oil
#13
Mohd Fahim, Mohammad Ibrahim, Sultan Zahiruddin, Rabea Parveen, Washim Khan, Sayeed Ahmad, Birendra Shrivastava, A K Shrivastava
INTRODUCTION: The absence of microbial growth and resistance to oxidative deterioration in fruits of Musa × paradisiaca L. (bananas) is an indication of the presence of antimicrobial and antioxidant metabolites. OBJECTIVE: In order to investigate the secondary metabolomic spectrum as well as the active antimicrobial and antioxidants present in essential oils (EOs) from fruits of different geographical areas of M. × paradisiaca, gas chromatography-mass spectroscopy (GC-MS) principal component data correlation analysis is complemented with antimicrobial assays and phytochemical and bioautographic antioxidant fingerprints with thin layer chromatography (TLC)...
January 4, 2019: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30588683/characterisation-and-classification-of-citri-reticulatae-pericarpium-varieties-based-on-uhplc-q-tof-ms-ms-combined-with-multivariate-statistical-analyses
#14
Yu-Ying Zheng, Xuan Zeng, Wei Peng, Zhong Wu, Wei-Wei Su
INTRODUCTION: Citri Reticulatae Pericarpium (CRP), comprising dried pericarps of Citrus reticulata Blanco and its cultivars, is popularly used for its great medicinal and dietary values. Generally, the pericarps from C. reticulate "Chachi" ("Guangchenpi" in Chinese, GCP) is considered to have superior qualities and merit premium price compared with CRP derived from other cultivars (collectively called "Chenpi" in Chinese, CP). Since its multiple origins and derived economic adulteration, it is significant to systematically compare the chemical profiles of different CRP varieties...
December 26, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30569602/a-dereplication-strategy-for-identifying-triterpene-acid-analogues-in-poria-cocos-by-comparing-predicted-and-acquired-uplc-esi-qtof-ms-ms-data
#15
Ye-Ting Zou, Fang Long, Cheng-Ying Wu, Jing Zhou, Wei Zhang, Jin-Di Xu, Ye-Qing Zhang, Song-Lin Li
INTRODUCTION: Triterpene acids from the dried sclerotia of Poria cocos (Schw.) Wolf (poria) were recently found to possess anti-cancer activities. Identification of more triterpene acid analogues in poria is worthwhile for high throughput screening in anti-cancer drug discovery. OBJECTIVE: To establish an efficient dereplication strategy for identifying triterpene acid analogues in poria based on ultra-performance liquid chromatography with electrospray ionisation quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-QTOF-MS/MS)...
December 19, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30569488/qualitative-and-quantitative-analysis-of-porana-sinensis-hemsl-by-uhplc-q-exactive-ms-tlc-autographic-method-and-dart-ms
#16
Zhiyong Chen, Mengmeng Wang, Yuanyuan Yang, Xia Du, Zijia Zhang, Ye Li
INTRODUCTION: Erycibe obtusifolia and E. schmidtii are widely used in traditional Chinese medicine (TCM) to treat joint pain and rheumatoid arthritis. With the reduction of wild E. obtusifolia and E. schmidtii resources, Porana sinensis has been widely used as a substitute. However, few studies have been conducted on the chemical composition and quality control of P. sinensis. OBJECTIVE: To clarify the chemical composition and improve the quality control of P. sinensis...
December 19, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30548356/discovery-and-characterisation-of-lycorine-type-alkaloids-in-lycoris-spp-amaryllidaceae-using-uhplc-qtos-ms
#17
Aiqian Li, Zhifeng Du, Mei Liao, Yulin Feng, Hanli Ruan, Hongliang Jiang
INTRODUCTION: Lycorine, one of the most common alkaloids in Lycoris spp., is believed to possess pharmacological activity. OBJECTIVE: To discover and identify lycorine-type alkaloids in the crude extracts of bulbs from six Lycoris spp. by ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) detection. METHODOLOGY: A qualitative analytical method with a data mining strategy was utilised. Based on the fragmentation patterns of standards investigated in positive tandem mass spectrometry (MS/MS) mode, the fragmentation rules of lycorine-type alkaloids were summarised...
December 11, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30548354/analysis-of-condensed-tannins-in-populus-spp-using-reversed-phase-uplc-pda-esi-ms-following-thiolytic-depolymerisation
#18
Kennedy F Rubert-Nason, Richard L Lindroth
INTRODUCTION: Condensed tannins (CTs) are proanthocyanidin heteropolymers that are widely distributed among plants. Their biochemical properties are determined by molecular structure (e.g. polymer size, hydroxylation, stereochemistry). In Populus, genetically and environmentally-determined CT concentrations have been related to ecological effects, while the potential role of CT molecular structure has received little attention. OBJECTIVE: Evaluate CT polymerisation, major constituent monomers, stereochemistry and overall content in Populus tremuloides foliage using ultra-high-performance liquid chromatography with photodiode array and mass spectrometry (UPLC-PDA-(-)esi-MS) detection following thiolytic depolymerisation of the CTs...
December 11, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30511497/chemical-fingerprint-and-bioactivity-evaluation-of-globularia-orientalis-l-and-globularia-trichosantha-fisch-c-a-mey-using-non-targeted-hplc-esi-qtof-ms-approach
#19
Celia Rodríguez-Pérez, Gokhan Zengin, Antonio Segura-Carretero, Devina Lobine, Mohamad Fawzi Mahomoodally
INTRODUCTION: In the quest for new sources of biologically-active compounds, the chemical, and biological profiles of two Globularia species (G. trichosantha Fisch. & C. A. Mey and G. orientalis L.) were investigated. METHODOLOGY: Chemical profiles were evaluated by high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS), as well as by their total phenolic, flavonoids, and phenolic acids contents...
December 3, 2018: Phytochemical Analysis: PCA
https://read.qxmd.com/read/30511406/simultaneous-qualitative-and-quantitative-evaluation-of-toddalia-asiatica-root-by-using-hplc-dad-and-uplc-qtof-ms-ms
#20
Mingjuan Zhu, Pinqing Wei, Qian Peng, Shengying Qin, Yuan Zhou, Ren Zhang, Chenchen Zhu, Lei Zhang
INTRODUCTION: Coumarin and alkaloids are the major bioactive constituents of Toddalia asiatica, playing an important role in various biological activities such as anti-inflammatory, analgesic, anti-bacterial and anti-tumour. OBJECTIVE: To establish a method that will simultaneously determine the coumarins and alkaloids compounds in T. asiatica and identify their characteristic fragmentation patterns, while combining fingerprints and chemical identification with chemometrics for discrimination and quality assessment of T...
December 3, 2018: Phytochemical Analysis: PCA
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